U.J. Lewis

Scripps Whittier Diabetes Institute

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W. P. VANDERLAAN

Scripps Whittier Diabetes Institute

B.K. Seavey

University of Manitoba

R. N. P. Singh

Scripps Whittier Diabetes Institute

Y. N. Sinha

Scripps Whittier Diabetes Institute

E. V. CHEEVER

Aetion (United States)

L. J. Lewis

Scripps Whittier Diabetes Institute

C.A. Elvehjem

University of Wisconsin–Madison

U. D. Register

Loma Linda University

Donald V. Tappan

United States Navy

Edith Markoff

Cincinnati Children's Hospital Medical Center

Cooperative Institutions

Scripps Health

Scripps Whittier Diabetes Institute

UCLA Health

Advertising Educational Foundation

New York University

Columbia University Irving Medical Center

Manhattan College

Scripps Clinic

American Standard (United States)

St. John's University

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Glycosylated ovine prolactin.

Proceedings of the National Academy of Sciences (1984)

U.J. Lewis Ravindra Singh L. J. Lewis B.K. Seavey Y. N. Sinha

A modification of prolactin, in which the asparagine at position 31 carries a carbohydrate unit, was isolated from ovine pituitary glands. Sequence and amino acid analyses identified the point of linkage of the carbohydrate. Glucosamine was found in acid hydrolysates, an indication that the carbohydrate is attached through N-acetylglucosamine. The glycosylated prolactin binds to concanavalin A and lentil lectin and is eluted with methyl alpha-D-mannopyranoside. During gel electrophoresis in sodium dodecyl sulfate, the glycosylated hormone migrates as a Mr 25,000 protein; prolactin has a Mr of 23,000. The modified prolactin had a potency of 20 international units/mg, approximately equal to 60% the potency of a reference prolactin preparation when measured by the pigeon crop sac assay. In a radioimmunoassay, the glycosylated form had only 34% the immunoreactivity of ovine prolactin.

Glycoconjugate

10.1073/pnas.81.2.385

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Citations (99)

A naturally occurring structural variant of human growth hormone.

Journal of Biological Chemistry (1978)

U.J. Lewis Joseph T. Dunn Lynda F. Bonewald B.K. Seavey W. P. VANDERLAAN

Human growth hormone

10.1016/s0021-9258(17)40874-x

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Citations (197)

Disk Electrophoresis of Basic Proteins and Peptides on Polyacrylamide Gels

Nature (1962)

R. Reisfeld U.J. Lewis Donald E. Williams

Polyacrylamide

10.1038/195281a0

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Citations (2,952)

Glycosylated Human Growth Hormone (hGH): A Novel 24 kDa hGH-N Variant

Biochemical and Biophysical Research Communications (1996)

Luis S. Haro U.J. Lewis Myrna Garcia Juan J. Bustamante Andrew Martinez

Sephadex

Glycoconjugate

10.1006/bbrc.1996.1697

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Citations (31)

PRACTICAL HUMAN GROWTH HORMONE. PREPARATION AND CLINICAL USE.

PubMed (1964)

W. P. VANDERLAAN U.J. Lewis A. E. Jones Jennifer Fisher

Human growth hormone was prepared from acetone-dried pituitary powder by hot glacial acetic acid extraction and subsequent precipitation by sodium chloride and cold acetone. The yield was 13 per cent and the preparation was called practical growth hormone in recognition of its complement of corticotropin. Treatment of two dwarfs with practical growth hormone in aqueous solution, 1 or 2 mg intramuscularly on alternate days, accelerated the growth rate and there were no physical signs or laboratory indications of adrenal stimulation or other adverse effects. The preparation is recommended for its safety, simplicity and relatively good yield.

Human growth hormone

Source

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Citations (1)

Variant forms of growth hormone and control of hormonal action

Springer eBooks (1988)

U.J. Lewis

Proteolysis

Deamidation

10.1007/978-94-009-1395-0_4

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Citations (1)

Studies of GH Secretion in Mice by a Homologous Radioimmunoassay for Mouse GH1

Endocrinology (1972)

Y. N. Sinha F. W. SELBY U.J. Lewis W. P. VANDERLAAN

A sensitive homologous radioimmunoassay for mouse GH capable of measuring concentrations of GH as low as 1 ng/ml of mouse serum has been developed. This assay has been used to study GH secretion in C3H mice under several physiological conditions. Newborn mice possessed very high concentration of GH in serum which persisted for two weeks. Adult male mice had more GH in their pituitary (66.9 μg/mg) and serum (12.0 ng/ml) than comparable females (48.9 μg/mg; 3.4 ng/ml). Ovariectomy in female mice increased pituitary and serum concentrations of GH to male levels whereas stilbestrol administration decreased it. Orchiectomy or stilbestrol treatment of male mice resulted in decreased GH levels. During the estrous cycle, maximum levels of GH were attained during proestrus. Insulininduced hypoglycemia or treatment with L—dopa failed to induce rise in serum concentrations of GH in mice.(Endocrinology91: 784, 1972)

Stilbestrol

10.1210/endo-91-3-784

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Citations (140)

Antithyroid Agents

Elsevier eBooks (1964)

W. P. VANDERLAAN A. E. Jones U.J. Lewis

10.1016/b978-1-4832-2846-4.50019-1

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Citations (0)

Human Growth Hormone: Preparation from Acetone Powder1

Endocrinology (1962)

Ralph A. Reisfeld U.J. Lewis Norman G. Brink S. L. Steelman

A simple method is described for the preparation of human growth hormone from acetone powders of pituitary glands. Growth hormone preparations were obtained in high yield and with biological activity equal to that of material made by published methods. The material obtained by this procedure proved entirely satisfactory in clinical studies. The only other hormonal activity observed in any concentration is that of prolactin. Human gonadotropins were also obtained in good yield from one of the side fractions.

Human growth hormone

10.1210/endo-71-4-559

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Citations (41)

Enzymatic Transformations of Growth Hormone and Prolactin

Journal of Biological Chemistry (1962)

U.J. Lewis

10.1016/s0021-9258(18)50134-4

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Citations (50)