Liang Xu

Zhujiang Hospital

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i-10 index

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Hong Qiu

Tongji Hospital

Pengbo Hu

Tongji Hospital

Yang Long

Huazhong University of Science and Technology

Wen‐Qing Lu

Shanghai University

Xiuyuan Zhang

Hebei North University

Qiang Zeng

Huazhong University of Science and Technology

Hao Yu

National University of Singapore

Yongyi Ma

Nantong University

Yanmei Luo

Army Medical University

Y. Li

Sichuan University

Cooperative Institutions

Huazhong University of Science and Technology

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Sciences

Shanghai Jiao Tong University

Zhejiang University

Army Medical University

Capital Medical University

Peking University

Southwest Hospital

Sun Yat-sen University

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High‐throughput 3D imaging of sperm

Molecular Reproduction and Development (2013)

Justin Su Liang Xu Derek Tseng Aydogan Özcan

Locomotion is an indispensable feature for animal sperm to fulfi ll their ultimate mission—delivering genetic material to eggs. Despite its signifi cance, we currently have limited knowledge about how human sperm move in three-dimensional (3D) space. To provide a high-throughput patform for tracking individual sperm, we developed a lensfree on-chip imaging technique (2012; PNAS. 109:16018) that can dynamically track the 3D trajectories of >1,500 individual human sperm inside an observation volume of 8–17 µL with submicron 3D positioning accuracy. The large statistics provided by this computational holographic imaging platform revealed that only 4–5% of the motile human sperm swim along 3D helices, and that this swimming pattern can be signifi cantly suppressed with seminal plasma. Further, a signifi cant majority (∼90%) of these helical human sperm, preferred right-handed helices over lefthanded ones, exhibiting a helix radius of 0.5–3 µm and a helical rotation speed of 3–20 rotations/sec. This high-throughput computational imaging platform could provide a valuable toolset for characterizing the statistical swimming patterns of various microorganisms, and might reveal new biophysics underlying their 3D motion.

10.1002/mrd.22159

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Clinical application of noninvasive prenatal testing in twin pregnancies: a single-center experience

Expert Review of Molecular Diagnostics (2023)

Yanmei Luo Bin Hu Yang Long Yan Pan Lupin Jiang

To evaluate the clinical efficiency of noninvasive prenatal testing (NIPT) for fetal chromosomal aneuploidy screening in twin pregnancies.A total of 1650 women with twin pregnancies were enrolled in the study, which underwent NIPT at the Southwest Hospital, Army Medical University, Chongqing, China from January 2013 to June 2022. Fetal karyotyping analysis was conducted in high-risk patients, with subsequent follow-up on pregnancy outcomes.In 1650 pregnancies, NIPT results showed ten cases of the fetal chromosome aneuploidy, of which six cases were true positive and four cases were false positive. The sensitivity, specificity, positive predictive value (PPV), and false-positive rate (FPR) of trisomy 21 were 100%, 99.79%, 57.14%, and 0.18%, respectively. Sensitivity, specificity, PPV, and FPR of trisomy 18 were 100%, 99.94%, 50%, and 0.06%, respectively. The sensitivity, specificity, PPV, and FPR of trisomy 13 were 100%, 100%, 100%, and 0%, respectively. No false negatives were detected and the negative predictive value (NPV) was 100% of the total. Eleven pregnancies failed the NIPT test with no-call due to the low fetal fraction (< 4%).NIPT is a high-performing routine primary prenatal screening test in twin pregnancies, with high sensitivity and specificity in screening for fetal aneuploidy.

Single Center

Twin Pregnancy

10.1080/14737159.2023.2193291

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Citations (1)

Sestrin2 induced by hypoxia inducible factor1 alpha protects the blood-brain barrier via inhibiting VEGF after severe hypoxic-ischemic injury in neonatal rats

Neurobiology of Disease (2016)

Xudan Shi Desislava Doycheva Liang Xu Jiping Tang Min Yan

Hypoxia

10.1016/j.nbd.2016.07.016

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Citations (55)

Association between the first and second trimester cell free DNA fetal fraction and spontaneous preterm birth

Expert Review of Molecular Diagnostics (2023)

Yanmei Luo Liang Xu Yongyi Ma X F Yan Renke Hou

To evaluate whether the fetal fraction of cell-free DNA at the first and second trimesters is associated with spontaneous preterm birth.

Cell-free fetal DNA

10.1080/14737159.2023.2217331

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Citations (2)

N-(4-(quinazolin-2-yl)phenyl)benzamide derivatives with potent anti-angiogenesis activities: synthesis and evaluation

Journal of the Iranian Chemical Society (2015)

Ming Sun Na Lv Zeng Li Qiru Xiong Liang Xu

Chorioallantoic membrane

Amide

Benzamide

Quinazoline

10.1007/s13738-015-0788-4

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Citations (1)

Pan-Cancer Analysis of OLFML2B Expression and Its Association With Prognosis and Immune Infiltration

Frontiers in Genetics (2022)

Pengbo Hu Xiuyuan Zhang Y. Li Liang Xu Hong Qiu

Background: The function of olfactomedin-like 2B (OLFML2B), as a member of the olfactomedin domain-containing protein family, remains ambiguous, especially in tumors. The current study explores the possible correlation between OLFML2B, prognosis, and immune infiltration in pan-cancer. Methods: We applied a number of bioinformatics techniques to probe the prospective function of OLFML2B, consisting of its association with prognosis, clinicopathology, alteration, GSEA, tumor microenvironment (TME), immune-associated genes, immune infiltration, tumor mutational burden (TMB), microsatellite instability (MSI), and drug sensitivity in several cancer types. qPCR and immunohistochemistry were used to identify OLFML2B expression in LIHC cell lines and liver cancer tissues. Results: We discovered that OLFML2B was overexpressed in 14 cancers and positively related to several cancer type prognoses. The expression of OLFML2B was further validated in the LIHC cell lines. OLFML2B expression was bound up with TMB in 13 cancers, MSI in 10 cancers, and TME in almost all cancers. Furthermore, OLFML2B was highly co-expressed with genes encoding immune activators and immune suppressors. We further found that OLFML2B played a role in infiltrating different types of immune cells, such as macrophages and cancer-associated fibroblasts. OLFML2B may influence various cancer and immune-related pathways, such as the PI3K-Akt signaling pathway, ECM-receptor interaction, focal adhesion, and leukocyte transendothelial migration. In addition, OLFML2B may increase drug resistance of binimetinib, cobimentinib, and trametinib. Conclusion: Our outcomes reveal that OLFML2B may act as a prognostic marker and a potential target in immunotherapy for diverse tumors due to its oncogenesis function and immune infiltration.

Microsatellite Instability

Immune checkpoint

10.3389/fgene.2022.882794

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Citations (9)

Drinking-Water Disinfection By-products and Semen Quality: A Cross-Sectional Study in China

Environmental Health Perspectives (2014)

Qiang Zeng Yixin Wang Shao‐Hua Xie Liang Xu Chen Yong-zhe

Background: Exposure to disinfection by-products (DBPs) has been demonstrated to impair male reproductive health in animals, but human evidence is limited and inconsistent.Objective: We examined the association between exposure to drinking-water DBPs and semen quality in a Chinese population.Methods: We recruited 2,009 men seeking semen analysis from the Reproductive Center of Tongji Hospital in Wuhan, China, between April 2011 and May 2012. Each man provided a semen sample and a urine sample. Semen samples were analyzed for sperm concentration, sperm motility, and sperm count. As a biomarker of exposure to drinking-water DBPs, trichloroacetic acid (TCAA) was measured in the urine samples.Results: The mean (median) urinary TCAA concentration was 9.58 (7.97) μg/L (interquartile range, 6.01–10.96 μg/L). Compared with men with urine TCAA in the lowest quartile, increased adjusted odds ratios (ORs) were estimated for below-reference sperm concentration in men with TCAA in the second and fourth quartiles (OR = 1.79; 95% CI: 1.19, 2.69 and OR = 1.51; 95% CI: 0.98, 2.31, respectively), for below-reference sperm motility in men with TCAA in the second and third quartiles (OR = 1.46; 95% CI: 1.12, 1.90 and OR = 1.30; 95% CI: 1.00, 1.70, respectively), and for below-reference sperm count in men with TCAA in the second quartile (OR 1.62; 95% CI: 1.04, 2.55). Nonmonotonic associations with TCAA quartiles were also estimated for semen parameters modeled as continuous outcomes, although significant negative associations were estimated for all quartiles above the reference level for sperm motility.Conclusion: Our findings suggest that exposure to drinking-water DBPs may contribute to decreased semen quality in humans.Citation: Zeng Q, Wang YX, Xie SH, Xu L, Chen YZ, Li M, Yue J, Li YF, Liu AL, Lu WQ. 2014. Drinking-water disinfection by-products and semen quality: a cross-sectional study in China. Environ Health Perspect 122:741–746; http://dx.doi.org/10.1289/ehp.1307067

Semen quality

Quartile

Interquartile range

Semen Analysis

10.1289/ehp.1307067

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Citations (61)

Identification of molecular pattern and prognostic risk model based on ligand-receptor pairs in liver cancer

Frontiers in Immunology (2023)

Pengbo Hu Liang Xu Yongqing Liu Xiuyuan Zhang Zhou Li

The tumor microenvironment of hepatocellular carcinoma is composed of multiple cells, and the interactive communication between cells drives tumor progression and characterizes the tumor. Communication between cells is mainly achieved through signal transduction between receptor ligands, and the rise of single-cell technology has made it possible to analyze the communication network between cells.We applied a train of bioinformatic techniques and in vitro experiments. We analyzed the composition of the microenvironment of liver cancer by combining single-cell sequencing data and transcriptome sequencing data from liver cancer to construct molecular typing and risk models for LRs. Then, we analyzed association of it with prognosis, mutation, KEGG, tumor microenvironment (TME), immune infiltration, tumor mutational burden (TMB) and drug sensitivity in liver cancer. qPCR and was used to identify SLC1A5 expression in LIHC cell lines and CCK8, transwell and cell colony formation were performed to validate the function of SLC1A5. Meanwhile, we also performed polarization of macrophages.In this experiment, we found that liver cancer tissues are rich in immune and mesenchymal cells, and there is extensive signaling between individual cells, so we constructed molecular typing and risk models for LRs. Combining clinical data revealed significant differences in clinical characteristics, prognosis and mutated genes between the molecular typing of receptor-ligand pairs, as well as in sensitivity to drugs; similarly, there were significant prognostic differences between the risk models. There were also notable differences in activated signaling pathways, infiltrating immune cells and immune subtypes. Subsequently, we used siRNA to knock down SLC1A5 in hepatocellular carcinoma cells and found that cell proliferation, migration and invasion were diminished.In conclusion, our LRs model may become a marker to guide clinical treatment and prognosis.

Liver Cancer

Immune checkpoint

10.3389/fimmu.2023.1187108

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Citations (3)

A transfer efficiency model for ultrasound mediated drug/gene transferring into cells

Ultrasonics Sonochemistry (2013)

Hao Yu Liang Xu Siping Chen

Ultrasound is a very promising technology to mediated drug/gene transferring into cells. However the relations between cell experimental conditions and results have been still unknown. It seriously impeded the development of the technology. In the article, a transfer efficiency model for ultrasound mediated drug/gene transferring into cells in stable cavitation was constructed. To testify the model, the numerical results were compared with the cell experimental data from six literatures in the entirely different experimental conditions. The numerical results fit the cell experimental data well. Despite simplifications and limitations, the model for the first time established the relationship between the cell experimental results about transfer efficiency and the conditions including ultrasound, microbubble and cells in stable cavitation.

Gene transfer

10.1016/j.ultsonch.2013.06.015

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Citations (5)

Current Progress on Predictive Biomarkers for Response to Immune Checkpoint Inhibitors in Gastric Cancer: How to Maximize the Immunotherapeutic Benefit?

Cancers (2023)

Yongqing Liu Pengbo Hu Liang Xu Xiuyuan Zhang Li Zhou

Gastric cancer is the fifth most prevalent cancer and the fourth leading cause of cancer death globally. Delayed diagnosis and pronounced histological and molecular variations increase the complexity and challenge of treatment. Pharmacotherapy, which for a long time was systemic chemotherapy based on 5-fluorouracil, is the mainstay of management for advanced gastric cancer. Trastuzumab and programmed cell death 1 (PD-1) inhibitors have altered the therapeutic landscape, contributing to noticeably prolonged survivorship in patients with metastatic gastric cancer. However, research has revealed that immunotherapy is only beneficial to some individuals. Biomarkers, such as programmed cell death ligand 1 (PD-L1), microsatellite instability (MSI), and tumor mutational load (TMB), have been shown to correlate with immune efficacy in numerous studies and are increasingly employed for the selection of patients most likely to respond to immunotherapy. Gut microorganisms, genetic mutations like POLE/POLD1 and NOTCH4, tumor lymphoid infiltrating cells (TILs), and other novel biomarkers have the potential to develop into new predictors. Prospective immunotherapy for gastric cancer should be guided by a biomarker-driven precision management paradigm, and multidimensional or dynamic marker testing could be the way to go.

Microsatellite Instability

Cancer Immunotherapy

Immune checkpoint

10.3390/cancers15082273

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Citations (9)