Background: The function of tropomyosin 2 (TPM2) in breast cancer is still far understudied. In this study, we aim to explore the roles of TPM2 in breast cancer progression. Methods: This research included 155 breast cancer tissues. The expression of TPM2 was analyzed by immunohistochemical staining and grading. The mRNA expression of TPM2 in pan-cancer was analyzed with The Cancer Genome Atlas (TCGA) data plate form. The differential expression of TPM2 protein and the differential promoter methylation level of TPM2 between breast cancer tissues and normal breast tissues were analyzed by the UALCAN online database. The relationship between TPM2 and signaling pathways was interpreted by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) pathway enrichment analyses. The survival curve of TPM2 was analyzed across the Kaplan–Meier plotter online database. Furthermore, the relationship between TPM2 expression and infiltrating macrophages was validated through in vitro co-culture experiments. Results: TPM2 expression was significantly down-regulated in breast cancer samples. In addition, TPM2 expression was correlated with lymph node metastasis and high-grade histopathological morphology. The receiver operating characteristic (ROC) curve indicated that TPM2 expression could well distinguish between normal breast tissue and breast cancer tissue. TPM2 may have potential value in breast cancer diagnosis. Bioinformatics analysis illustrated that TPM2 was mainly involved in extracellular matrix organization, collagen fibril organization, cell junction assembly, focal adhesion, cAMP signaling pathway, estrogen signaling pathway, Wnt signaling pathway, and adaptive immune system. TPM2 expression was correlated with immune infiltrating cells and immune checkpoint molecules. Our in vitro co-culture experiments showed that the M2 macrophages could upregulate the expression of TPM2. Conclusion: TPM2 may play key roles in breast cancer occurrence and development, especially in cancer metastasis. TPM2 may be a potential biomarker for breast cancer diagnosis. Keywords: breast cancer, TPM2, bioinformatics, cytoskeleton
Purpose: While previous cross-sectional studies have investigated the relationship between problematic online video watching and mental health issues, longitudinal studies exploring their bidirectional relationship remain relatively scarce. This study aimed to fill this gap by conducting a two-wave longitudinal design. Participants and Methods: Six hundred and ninety-nine first-year undergraduates voluntarily completed two rounds of questionnaire surveys assessing problematic online video watching, boredom proneness and loneliness with an interval of 4 months. To test the research hypotheses, cross-lagged panel models were performed using AMOS 24.0. Results: Our results revealed the that (1) Boredom proneness and problematic online video watching were mutually influenced. (2) Loneliness at T1 positively predicted the level of problematic online video watching at T2 but not vice versa. (3) Boredom proneness mediated the link between loneliness and problematic online video watching. (4) Chinese first-year undergraduates' levels of problematic online video watching and boredom proneness significantly increased during their educational transition period from high school to university. Conclusion: The present study enhances our understanding of the dynamic relationships between problematic online video watching, boredom proneness and loneliness, highlighting the targeted prevention and interventions for first-year undergraduates. Keywords: problematic online video watching, boredom proneness, loneliness, cross-lagged panel model
The assessment of inflammation activity in ulcerative colitis (UC) includes endoscopy and histology. Confocal laser endomicroscopy (CLE) combines real-time endoscopy and histology. This study was aimed at evaluating the application of CLE in the assessment of inflammation activity in UC.In total, 73 consecutive patients with UC who visited Qilu Hospital for colonoscopy surveillance underwent CLE. Inflammation activity was first assessed by the colonoscopy Baron score, then by CLE with a 4-grade classification of crypt architecture, as well as by analysis of microvascular alterations and fluorescein leakage. Targeted biopsy samples were taken for histological analysis. Stored CLE images were subjected to post-CLE objective assessment.Both assessment of crypt architecture and fluorescein leakage with CLE showed good correlations with histological results (Spearman's rho, both P<0.001). CLE seemed to be more accurate than conventional white-light endoscopy for evaluating macroscopical normal mucosa. More than half of the patients with normal mucosa seen on conventional white-light endoscopy showed acute inflammation on histology, whereas no patients with normal mucosa or with chronic inflammation seen on CLE showed acute inflammation on histology. Assessment of microvascular alterations by CLE showed good correlation with histological findings (P<0.001). On post-CLE objective assessment, subjective architectural classifications were supported by the number of crypts per image (P<0.001) but not fluorescein leakage results by gray scale (P=0.194).CLE is reliable for real-time assessment of inflammation activity in UC. Crypt architecture, microvascular alterations, and fluorescein leakage are promising markers in CLE evaluation.
In this paper,the characteristic of CAPP process resource data is analyzed,its model is established;the enterprise's requirement for its management is researched,combining with the requirement and characteristic of its management,the structure of applied CAPP process resource database is put forword,its management is carried out by using ODBC,and the speediness multi-condition query for process data is offered,efficiency management and using for process data is achieved.
This paper investigated the structure and content of job insecurity, as well as the antecedents on it and inner mechanism. Using a sample of 468 manufacturing employees from Suzhou, Nanjing, Shenzhen, Xi'an and other cities in China, we found: The construct of job insecurity is consisted of three factors, including quantitative job insecurity, salary promotion insecurity and interpersonal insecurity. On some dimensions of job insecurity, there are significant differences in gender, age educational level, etc. The "cooperation and results-oriented", one dimension of Person-Organization Fit, had direct negative effect on quantitative job insecurity; The "contribution", one dimension of Leader-Member Exchange, has played a moderating role between them. Some suggestions were provided behind.
Objective To explore expression of villin in normal gastric mucosa tissues,intestinal metaplasia(IM),dysplasia,and gastric carcinoma and its clinical significance.Methods An immunohistochemical method was used to determine villin expression.Results Villin was expressed in dysplasia cells but not in gastric mucosa.The positive rates of its expression in intestinal metaplasia,dysplasia,and gastric carcinoma were 85.0%(17/20),66.7%(20/30),and 48.3%(29/60),respectively.It was also found that expression of villin had no relationship to tumor size,invasiveness,and clinical stage and metastasis to lymphnodes(P0.05),however,expression of villin was significantly higher in well moderately differentiated cancers than in poorly differentiated ones(P0.05).Conclusion Expression of villin in precancerous lesions and gastric carcinoma is down-regulated and is closely related to tumor differentiation degrees.Villin is a good marker for screening different types of gastric carcinoma and has a certain guiding sense to early diagnosis and clinical treatment.
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