The mode of action of probiotics is still incompletely understood. To study the interactions between probiotic micro-organisms and the host their effects on morphology and mucins of the intestinal mucosa were investigated. Fifteen clinically healthy weaned pigs were divided into three groups and received either Saccharomyces boulardii or Bacillus cereus var. toyoi or were left untreated. Sections of duodenum, proximal and mid jejunum, ileum, caecum, and colon were examined. An increase of villus length in the small intestine and a decrease in the number of goblet cells with 2.6-sialylated mucins in the large intestine were observed in both treatment groups. There were no differences in crypt morphology, number of Ki67-positive cells, total number of goblet cells and number of goblet cells with acidic, neutral, sulphated, or 2.3-sialylated mucins between groups. The results indicate an effect of Saccharomyces boulardii and Bacillus cereus var. toyoi on the intestinal architecture of pigs.

Saccharomyces boulardii

Jejunum

Mucin 2

Caecum

Large intestine

Goblet cell

Cecum

Intestinal mucosa

10.1055/s-2002-30116

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Citations (75)

Biochemical and histochemical study on the intestinal mucosa of the common carp Cyprinus carpio L., with special consideration of mucin glycoproteins

Journal of Fish Biology (2007)

H. Neuhaus M. van der Marel N Caspari Wieland Meyer M.‐L. Enss

The biochemical and histochemical properties of intestinal mucin glycoproteins of virus and parasite‐free common carp Cyprinus carpio were investigated. The presence of carbohydrates in mucin glycoproteins could be demonstrated by histochemical methods, but generally, no obvious differences in specific staining for mucin glycoproteins were observed in contrast to biochemical techniques. Biochemical staining methods displayed differences in structure and composition of intestinal glycoproteins. Released intestinal glycoproteins contained two types of mucin glycoproteins: type 1 mucins displayed a size of >2000 kDa, and were highly glycosylated, while type 2 mucins ranged between 700 and 70 kDa, and were weakly glycosylated. In epithelial (intracellular) glycoproteins, mainly N‐acetyl‐α‐galactosamine and mannose were found, while in luminal (extracellular) glycoproteins in addition sialic acid was evident. Fucose was not detected. Thus, structure and composition of intestinal glycoproteins of common carp were similar to those found in mammals.

Common carp

10.1111/j.1095-8649.2007.01438.x

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Citations (56)

Effects of PGE2 on amount and composition of high molecular weight glycoproteins released by human gastric mucous cells in primary culture

Prostaglandins Leukotrienes and Essential Fatty Acids (1997)

M.‐L. Enss Siegfried Wagner U. Schmidt-Wittig H.‐K. Heim Winfried Beil

10.1016/s0952-3278(97)90503-2

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Citations (10)

Differenzielle Muzinexpression im Gastrointestinaltrakt

Zeitschrift für Gastroenterologie (2001)

Markus Cornberg M.‐L. Enss S A Wagner

Under physiological conditions mucins display a tissue specific expression. MUC2, MUC3, MUC5AC and MUC6 are the major mucins in the gastrointestinal tract. In the intestinal tissue MUC2 and MUC3 are the predominant mucins, whereas MUC5AC and MUC6 are the most important mucins in the stomach. Pathophysiological conditions are characterized by an aberrant gene expression. This includes the expression of non-tissue specific mucins as well as a reduced expression of the specific mucins. During inflammation a co-expression of non-tissue specific mucins was observed. Neoplastic transformations are also associated with an aberrant mucin expression. Obviously, an increased expression of non-specific mucins is related with a more favorable prognosis, while a decreased mucin expression is indicative for an increased cellular dedifferentiation and a poor prognosis. This modified mucin expression may result from a modified gene expression as well as from modifications in the posttranscriptional processing. A modified mucin expression reflects pathophysiological conditions and may support the pathohistological diagnosis.

Mucin 2

Pathophysiology

10.1055/s-2001-13710

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Citations (1)

Prostaglandin E2 alters terminal glycosylation of high molecular weight glycoproteins, released by pig gastric mucous cells in vitro

Prostaglandins Leukotrienes and Essential Fatty Acids (1995)

M.‐L. Enss U. Schmidt-Wittig H.‐K. Heim K.‐Fr. Sewing

10.1016/0952-3278(95)90035-7

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Citations (13)