Arseny S. Kaprelyants

A N Bach Institute of Biochemistry

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Margarita O. Shleeva

Russian Academy of Sciences

Elena G. Salina

A N Bach Institute of Biochemistry

Douglas B. Kell

Technical University of Denmark

Galina V. Mukamolova

University of Leicester

Galina R. Demina

A N Bach Institute of Biochemistry

Tatyana Azhikina

Institute of Bioorganic Chemistry

Vadim Nikitushkin

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)

Michael Young

New York Academy of Sciences

Alexander Apt

Central Scientific Research Institute of Tuberculosis Russian Academy of Medical Sciences

Dmitriy Ignatov

Max Planck Unit for the Science of Pathogens

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Russian Academy of Sciences

103

A N Bach Institute of Biochemistry

Federal Center Research Fundamentals of Biotechnology

Lomonosov Moscow State University

Central Scientific Research Institute of Tuberculosis Russian Academy of Medical Sciences

Institute of Bioorganic Chemistry

Aberystwyth University

Ministry of Health of the Russian Federation

G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms

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Effect of Photodynamic Inactivation against Dormant Forms and Active Growing Cells of Mycobacterium smegmatis

Applied Biochemistry and Microbiology (2020)

Margarita O. Shleeva Alexander P. Savitsky Vadim Nikitushkin I. D. Soloviev К. А. Трутнева

Mycobacterium smegmatis

Bacterial growth

10.1134/s000368382003014x

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Citations (13)

Role of Small Noncoding RNAs in Bacterial Metabolism

Biochemistry (Moscow) (2015)

Tatyana Azhikina Dmitriy Ignatov Elena G. Salina Mikhail V. Fursov Arseny S. Kaprelyants

Transcription

Riboswitch

10.1134/s0006297915130015

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Citations (10)

P2000 The muralitic activity of Rpf proteins and their ability to resuscitate dormant mycobacterial cells

International Journal of Antimicrobial Agents (2007)

Galina R. Demina Galina V. Mukamolova Arseny S. Kaprelyants

10.1016/s0924-8579(07)71839-3

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Citations (0)

Additional file 6: Table S6. of Dormant non-culturable Mycobacterium tuberculosis retains stable low-abundant mRNA

Figshare (2015)

Dmitriy Ignatov Elena G. Salina Mikhail Fursov Timofey Skvortsov Tatyana Azhikina

Functional enrichments analysis. Functional enrichment analysis was performed using the GOseq package [76] and functional categories from the TubercuList and PATRIC databases [33, 34]. The lists of differentially expressed (up- and down-regulated) genes were generated by the comparison of the following samples: DE vs. Log, DM vs. DE, and DL vs. DM. For each functional category, the number of differentially expressed genes, the total number of genes in the category, and the adjusted p-value of overrepresentation are shown. (XLSX 38 kb)

Table (database)

10.6084/m9.figshare.c.3613415_d7

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Rpf proteins are the factors of reactivation of the dormant forms of actinobacteria

Biochemistry (Moscow) (2016)

Vadim Nikitushkin Galina R. Demina Arseny S. Kaprelyants

10.1134/s0006297916130095

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Citations (33)

PREVENTION OF TUBERCULOSIS: CURRENT APPROACHES TO DEVELOPMENT OF VACCINES

Annals of the Russian academy of medical sciences (2012)

Marina Stukova Natalia Zabolotnykh Т. И. Виноградова V. Ya. Gergert Alexander Apt

This review is focused on recent advances in development of new vaccines for the prevention of tuberculosis. The main reasons for lack of BCG vaccine efficacy in different populations and geographic regions are presented. Design of new vaccines based on live modified strains of Mycobacterium bovis BCG, attenuated strains of Mycobacterium tuberculosis, recombinant proteins and viral vectors is considered in the specific examples. The usage of the heterologous «prime-boost» vaccination strategy against tuberculosis is discussed.

Heterologous

Tuberculosis vaccines

BCG vaccine

Attenuated vaccine

10.15690/vramn.v67i11.471

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Proteins of the Rpf (resuscitation promoting factor) family are peptidoglycan hydrolases

Biochemistry (Moscow) (2006)

M. V. Telkov Galina R. Demina S. A. Voloshin Elena G. Salina T. V. Dudik

Micrococcus luteus

10.1134/s0006297906040092

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Quantitative Analysis of the Physiological Heterogeneity within Starved Cultures of Micrococcus luteus by Flow Cytometry and Cell Sorting

Applied and Environmental Microbiology (1996)

Arseny S. Kaprelyants Galina V. Mukamolova Hazel M. Davey Douglas B. Kell

A high proportion of Micrococcus luteus cells in cultures which had been starved for 3 to 6 months lost the ability to grow and form colonies on agar plates but could be resuscitated from their dormancy by incubation in an appropriate liquid medium (A. S. Kaprelyants and D. B. Kell, Appl. Environ. Microbiol. 59:3187-3196, 1993). We used flow cytometry and cell sorting to study populations of bacteria that had been starved for 5 months. These cells could be stained by the fluorescent lipophilic cation rhodamine 123, but such staining was almost independent of metabolically generated energy in that it was not affected by uncouplers. Two populations could be distinguished, one with a lower degree of rhodamine fluorescence (a degree of fluorescence referred to as region A and containing approximately 80% of the cells) and one with a more elevated degree of fluorescence (region B, approximately 20% of the cells). Subsequent incubation of starved cells in fresh medium in the presence of the antibiotic chloramphenicol (to which M. luteus is sensitive) resulted in the transient appearance of cells actively accumulating rhodamine 123 (and fluorescing in region B) and of larger cells exhibiting a yet-greater degree of fluorescence (region C). These more fluorescent cells accounted for as much as 50% of the total population, under conditions in which the viable and total counts were constant. Thus, metabolic resuscitation of at least one-half of the cells takes place under conditions in which cryptic growth cannot play any role. Sorting experiments revealed that the great majority of the viable cells in the starved population are concentrated in regions B and C and that the extent of rhodamine staining under conditions of starvation therefore reflects the physiological state of the cells. Physical separation of these cells from cells in region A resulted in an increase (of approximately 25-fold) in the viability of cells in regions B and C and of the population as a whole. Resuscitation of dormant cells in a most-probable-number assay in the presence of supernatant taken from growing M. luteus revealed the resuscitation of cells from regions B and C but not from region A. It is suggested that initially dormant (resuscitable) cells are concentrated in regions B and C.

Micrococcus luteus

Rhodamine 123

Cell Sorting

Rhodamine

Cytometry

10.1128/aem.62.4.1311-1316.1996

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Citations (74)

Muralytic activity of Micrococcus luteus Rpf and its relationship to physiological activity in promoting bacterial growth and resuscitation

Molecular Microbiology (2005)

Galina V. Mukamolova Alexey G. Murzin Elena G. Salina Galina R. Demina Douglas B. Kell

The culturability of several actinobacteria is controlled by resuscitation-promoting factors (Rpfs). These are proteins containing a c. 70-residue domain that adopts a lysozyme-like fold. The invariant catalytic glutamate residue found in lysozyme and various bacterial lytic transglycosylases is also conserved in the Rpf proteins. Rpf from Micrococcus luteus, the founder member of this protein family, is indeed a muralytic enzyme, as revealed by its activity in zymograms containing M. luteus cell walls and its ability to (i) cause lysis of Escherichia coli when expressed and secreted into the periplasm; (ii) release fluorescent material from fluorescamine-labelled cell walls of M. luteus; and (iii) hydrolyse the artificial lysozyme substrate, 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside. Rpf activity was reduced but not completely abolished when the invariant glutamate residue was altered. Moreover, none of the other acidic residues in the Rpf domain was absolutely required for muralytic activity. Replacement of one or both of the cysteine residues that probably form a disulphide bridge within Rpf impaired but did not completely abolish muralytic activity. The muralytic activities of the Rpf mutants were correlated with their abilities to stimulate bacterial culturability and resuscitation, consistent with the view that the biological activity of Rpf results directly or indirectly from its ability to cleave bonds in bacterial peptidoglycan.

Micrococcus luteus

Micrococcus

10.1111/j.1365-2958.2005.04930.x

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Citations (219)

On the molecular mechanism of nonspecific antimicrobial action of protonated diallylammonium polymers on mycobacterial cells

European Polymer Journal (2022)

L. М. Timofeeva Г. Н. Бондаренко Vadim Nikitushkin Yulia A. Simonova Maxim A. Topchiy

Mycobacterium smegmatis

10.1016/j.eurpolymj.2022.111214

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Citations (6)