Adissa Tran

Ecologie Microbienne Lyon

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Christopher Payan

Inserm

Arnaud Gagneur

Centre Hospitalier Universitaire de Sherbrooke

Philippe Descamps

École Nationale Supérieure d'Ingénieurs de Caen

Lucie Auzanneau

Roche (France)

Laurent Andréoletti

Université de Reims Champagne-Ardenne

Julien Caer

Institut de Virologie

Marie‐Christine Legrand‐Quillien

Centre Hospitalier Régional Universitaire de Brest

Hélène Ansquer

Centre Hospitalier Régional Universitaire de Brest

E. Postec

Laboratoire des Biomolécules

Françoise Tourmen

Service de la Santé Publique

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Centre Hospitalier Régional Universitaire de Brest

Inserm

Institut de Virologie

Université de Bretagne Occidentale

Université de Reims Champagne-Ardenne

Centre Hospitalier Universitaire d'Angers

Centre National de la Recherche Scientifique

Centre Hospitalier Universitaire de Reims

Hôpital Robert-Debré

Université Bretagne Loire

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Human papillomaviruses : origin, oncogenic factors and markers for cervical cancer screening

Virologie (2024)

A. Grange Victor Mouton Mael Roojee S. Rosec Adissa Tran

The Human papillomaviruses (HPV) have existed in the human population since the archaic hominids. Over the course of human migration and evolution, HPVs have co-evolved with humans on all continents to become today the leading cause of cervical cancer. HPVs are classified by genera, species, genotype, lineage, sub-lineage and variants. Among more than 200 HPV genotypes, HPV16 is the most common and the most oncogenic at high-risk (HPV-HR). If viral oncogenesis is governed by numerous factors and mechanisms involving the virus and its host, the E6/E7 oncogenic proteins of HR-HPV play a central role and are always expressed in cervical cancers. Those of HPV16 have the greatest affinity for cellular proteins involved in cellular control, p53/E6 and pRb/E7. Some E6/E7 HPV16 mutants are associated with persistent infection, correlating with viral lineages and their ethnic and geographical origin. If the splicing of viral mRNAs encoding E6/E7 allows the overexpression of the E7 protein, which is an essential condition for the establishment of HR-HPV oncogenesis, other mechanisms contribute to strengthening it. On the one hand, the accidental integration of the viral genome, in particular the E1/E2 coding region, participates in the transformation of the infected cell. On the other hand, hypermethylation of the viral L1/L2 genomic regions is observed in the advanced stages of infection. Different methods for analyzing mutations, splice sites, integrations and methylations of the viral genome have been described. These virological markers could complete the detection of HR-HPV in the context of cervical cancer screening, currently recommended in France.

Papillomaviridae

10.1684/vir.2024.1069

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Transmission du cytomégalovirus chez les enfants prématurés par le lait maternel cru

Cahiers de Nutrition et de Diététique (2006)

Séverine Croly-Labourdette Arnaud Gagneur Sophie Vallet G. Gremmo-Féger Marie‐Christine Legrand‐Quillien

10.1016/s0007-9960(06)70625-3

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Human Papillomavirus Quantification in Urine and Cervical Samples by Using the Mx4000 and LightCycler General Real-Time PCR Systems

Journal of Clinical Microbiology (2007)

Christopher Payan Alexandra Ducancelle Mohamed Houmed Aboubaker Julien Caer Malena Tapia

ABSTRACT During the last decade, growing efforts have focused on human papillomavirus (HPV) detection using liquid hybridization, conventional PCR, and real-time PCR-based methods to increase the overall proportion of patients participating in cervical cancer screening procedures. We proposed a new general HPV DNA real-time PCR on the Mx4000 (Stratagene) and LightCycler (Roche Diagnostics) systems usable for both cervical scrape specimens and urine samples. A linear range was obtained from 5 DNA copies to 8 log 10 DNA copies/ml, and intra- and interassay variations were between 1.8 and 4%. Cervical carcinoma and HPV DNA screening was performed in 333 individual women referred for gynecological examination at the university hospitals of Angers and Brest and enrolled in the PapU study. Among cervical specimens ( n = 333), 45% were positive for HPV DNA, with a mean viral load at 5.00 log/ml (± 1.73). Among urine samples ( n = 177), 37% were positive with a significant 50-fold-lower mean viral load (3.77 ± 1.32 log/ml; P < 0.0001). Kappa agreement for HPV DNA between cervical and urine specimens was excellent (93%). Thus, we developed a highly sensitive and quantitative general HPV DNA real-time PCR method that allows mass screening of patients with HPV infection. The ongoing longitudinal and prospective multicenter PapU study should give us the opportunity to validate this method adapted to HPV DNA screening in urine samples in a larger population.

Papillomaviridae

10.1128/jcm.02022-06

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Citations (74)

Prevalence of Rotavirus, Adenovirus, Norovirus, and Astrovirus Infections and Coinfections among Hospitalized Children in Northern France

Journal of Clinical Microbiology (2010)

Adissa Tran Déborah Talmud B. Lejeune Nicolas Jovenin Fanny Renois

From January to December 2007, 973 stool specimens were prospectively collected from children hospitalized for gastroenteritis signs or from neonates and premature cases who were born in two French hospital settings in the north of France. They were tested by rapid enzyme immunoassay (EIA) analyses for rotavirus and adenovirus and by two commercially available ELISA tests for the detection of norovirus and astrovirus. The overall rates of prevalence for rotavirus, norovirus, adenovirus, and astrovirus were 21, 13, 5, and 1.8%, respectively, and they did not significantly differ between the two hospital settings (P=0.12). Mixed virus infections were detected in 32 (3.3%) of the 973 study children and were associated with norovirus in 21 (66%) infants, including 5 premature cases. From fall to spring, norovirus infections accounted for 52% of documented gastroenteritidis viral infections at a time when rotavirus was epidemic, resulting in mixed norovirus and rotavirus gastrointestinal tract infections. Of the 367 documented viral gastroenteritis cases, 15 (4.1%) were identified as nosocomial infections, 5 of which occurred in premature cases. These findings highlight the need to implement norovirus and astrovirus ELISA detection assays in association with rapid EIA rotavirus and adenovirus detection assays for the clinical diagnosis and the nosocomial prevention of gastroenteritis viral infections in pediatric departments.

Astrovirus

Rotavirus vaccine

10.1128/jcm.02181-09

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Citations (150)

Impact of rotavirus vaccination on hospitalizations for rotavirus diarrhea: The IVANHOE study

Vaccine (2011)

Arnaud Gagneur Emmanuel Nowak Thomas Lemaître Jean-François Segura N. Delapérrière

Rotavirus vaccine

10.1016/j.vaccine.2011.03.035

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Citations (70)