Andreas Ruppel

To detect the infection of Schistosoma japonicum in mice with a novel test based on agglutination of hybridoma cells and to study the mechanism of the hybridoma cells agglutination.The procedure was developed with a murine cell line H226 producing a monoclonal antibody specific to schistosome 31/32 kDa antigen and sera collected from mice infected with different numbers (10, 30, 50) of S. japonicum cercariae in different period. Immunofluorescent test was carried out with the hybridoma cells and schistosome-infected sera.The circulating antigen was detected by the test as early as 2 weeks after a heavy infection and all mice showed positive results in the test by 5 weeks after infection. The titers of antigen rose along with the time post infection, and the titers of sera from heavy infection were statistically higher than that from the mice receiving a lower number of cercariae. Specific yellowish green fluorescence appeared on the membrane of the hybridoma cells; no signal was detected inside.Hybridoma cell agglutination test (HCAT) may become useful to diagnose schistosomiasis.

Direct agglutination test

SCHISTOSOMIASIS JAPONICA

Agglutination (biology)

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Diagnostic Mr31/32 000 Schistosoma mansoni proteins (Sm31/32): Reaction with sera from Sudanese patients infected with S. mansoni or S. haematobium

Journal of Helminthology (1988)

Mohamed A. Idris Andreas Ruppel

ABSTRACT Sera of Sudanese patients with active infections of Schistosoma mansoni or S. haematobium were tested in immunoblots for their reactivity with Mr31/32 000 proteins of adult S. mansoni (Sm31/32). All sera from patients with intestinal (n=123) and all but one from those with urinary schistosomiasis (n=35) had antibodies against Sm31/32. These and additional data suggest that both specificity and sensitivity of Sm31/32 to detect schistosome infections are close to 100%. Antibodies against these proteins developed also in monkeys after experimental infection with S. haematobium . Sm31/32 antigens reacted in immunoblots as a doublet with most S. haematobium -patient sera and as a broad band with many S. mansoni -sera suggesting that at least two components are present in the molecular weight region of Mr31/32 000. The data demonstrate the potential use of Sm31/32 from adult worms to diagnose patients with intestinal or urinary schistosomiasis in endemic areas.

Schistosoma

10.1017/s0022149x00011305

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Effizientes Lernen von ICD-Klassifikationen anhand von Diagnosetexten

Informatik aktuell (1992)

Wolfram Pietsch Andreas Ruppel Bernd Schneider

10.1007/978-3-642-77447-8_21

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Schistosoma mansoni and Schistosoma haematobium: Identification and Characterization of Glycoconjugate Antigens in the Hemolymph of Infected Vector Snails

Journal of Parasitology (2002)