Yui TAKEYAMA

Obihiro University of Agriculture and Veterinary Medicine

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Ruby Mojica SILVA

Institute of Biomedical Science

Ana Yimiko KOJIMA

Universidade Federal de Mato Grosso do Sul

Kiyoshi MIYAZAWA

Unicharm (Japan)

Kunitada SATO

Kurume University

Tomas Javier ACOSTA AYALA

Obihiro University of Agriculture and Veterinary Medicine

Mitsuhiro TAKAGI

Obihiro University of Agriculture and Veterinary Medicine

Missaka Priyadarsana Bandara WIJAYAGUNAWARDANE

University of Peradeniya

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Obihiro University of Agriculture and Veterinary Medicine

Universidade Federal de Mato Grosso do Sul

Gifu University

Cambridge Life Sciences (United Kingdom)

University of Peradeniya

Institute of Biomedical Science

Universidade de São Paulo

Unicharm (Japan)

Life Science Institute

Shiseido Group (Japan)

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Estimating Insemination Time in Mares on the Basis of the Urinary Concentration of Estrone-Sulphate.

Journal of Equine Science (1998)

Ruby Mojica SILVA Yui TAKEYAMA Tomas Javier ACOSTA AYALA Missaka Priyadarsana Bandara WIJAYAGUNAWARDANE Ana Yimiko KOJIMA

The present study aimed to estimate the ovulation time in the mare on the basis of changes in urinary estrone sulphate (E1S) concentrations by using an EIA kit, originally used for plasma. Urine samples were collected from mares during the preovulatory period until ovulation. The urinary E1S concentrations showed a gradual increase from 153 ± 45 ng/ml urine and 210 ± 22 ng/mg creatinine (Cr) at D-5 and then reached a peak of 316 ± 43 ng/ml urine and 394 ± 35 ng/mgCr at D-2 in 81% of the mares in both groups. These findings indicate that daily determination of urinary E1S concentrations allows us to estimate the ovulation day and subsequently decide the optimum mating service time in mares.

10.1294/jes.9.125

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