To investigate the changes of circulating levels of all proglucagon-derived peptides (PGDPs) in individuals with overweight or obesity receiving liraglutide (3 mg) or naltrexone/bupropion (32/360 mg), and to explore the association between induced changes in postprandial PGDP levels and body composition, as well as metabolic variables, after 3 and 6 months on treatment.
Heteropneustes fossilis were subjected to high and low doses of the latex of Euphorbia royleana for short-term and long-term exposures, respectively. The blood was analyzed for plasma calcium levels, and the ultimobranchial glands fixed and examined. Serum calcium levels declined after 48 h following short-term exposure, and this decrease continued until the end of the experiment. The ultimobranchial cells exhibited a decrease in the cytoplasmic staining response after 96 h following treatment, and the nuclear volumes slightly decreased. Chronically exposed fish also exhibited a decline in serum calcium levels, but much later, on day 7, again progressively declining until the close of the experiment. Up to day 14 following treatment there was no change in histological structure of the ultimobranchial glands, but then there was a decrease in nuclear volume and the cytoplasm displayed a weak staining response. There was some vacuolization and degeneration.Keywords: Botanicals, calcitonin, calcium, teleost
Pregnancy-associated plasma protein-A2 (PAPP-A2) is a metalloproteinase that cleaves IGFBP-3 and IGFBP-5. Human mutations in PAPPA2 result in short stature with a low percentage of free IGF-I. Little is known about PAPP-A2 levels and the regulation of free IGF-I throughout childhood. We examined PAPP-A2 and intact IGFBP-3 levels in childhood and explored associations between PAPP-A2, free and total IGF-I, and total and intact IGFBP-3 and their relationship to the percentage of free to total IGF-I and anthropometric factors.Cross-sectional study at a single center.PAPP-A2, free IGF-I, and intact IGFBP-3 levels were measured in childhood (3-18 years old) and an evaluation of the relationship between these proteins and anthropometric factors.In 838 children, PAPP-A2 consistently decreased throughout childhood. In contrast, free IGF-I increased. A pubertal peak in free IGF-I was present in females but was less evident in males. Intact and total IGFBP-3 increased throughout childhood; however, intact IGFBP-3 had a more marked rise than total IGFBP-3. Percent free IGF-I decreased with no distinct pubertal peak. PAPP-A2 levels positively correlated with the percent free IGF-I (Male, Female; r = 0.18, 0.38; P < 0.001) and negatively with intact IGFBP-3 (Male, Female; r = -0.58, -0.65; P < 0.0001).This is the first study to describe serum PAPP-A2 and intact IGFBP-3 in children between 3 and 18 years of age. Our correlative findings suggest that PAPP-A2 is an important regulator of the percent free IGF-I which can be a marker of perturbations in the GH/IGF-I axis.
Abstract Context The oocyte-secreted factors growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) play essential roles in follicle development and oocyte maturation, and aberrant regulation might contribute to the pathogenesis of polycystic ovary syndrome. Objective Are there measurable differences in concentrations of GDF9, BMP15, and the GDF9/BMP15 heterodimer in small antral follicle fluids from women with and without polycystic ovaries (PCO)? Design and Setting Follicle fluids (n = 356) were collected from 4- to 11-mm follicles in unstimulated ovaries of 87 women undergoing ovarian tissue cryopreservation for fertility preservation. Patients Twenty-seven women with PCO were identified and 60 women without PCO-like characteristics (non-PCO women) were matched according to age and follicle size. Main outcome measures Intrafollicular concentrations of GDF9, BMP15, GDF9/BMP15 heterodimer, anti-Mullerian hormone (AMH), inhibin-A and -B, total inhibin, activin-B and -AB, and follistatin were measured using enzyme-linked immunosorbent assays. Results The detectability of GDF9, BMP15, and the GDF9/BMP15 heterodimer were 100%, 94.4%, and 91.5%, respectively, and concentrations were significantly negatively correlated with increasing follicle size (P < 0.0001). GDF9 was significantly higher in women with PCO (PCO: 4230 ± 189 pg/mL [mean ± SEM], n = 188; non-PCO: 3498 ± 199 pg/mL, n = 168; P < 0.03), whereas BMP15 was lower in women with PCO (PCO: 431 ± 40 pg/mL, n = 125; non-PCO: 573 ± 55 pg/mL, n = 109; P = 0.10), leading to a significantly higher GDF9:BMP15 ratio in women with PCO (P < 0.01). Significant positive associations between BMP15 and AMH, activins, and inhibins in non-PCO women switched to negative associations in women with PCO. Conclusions Intrafollicular concentrations of GDF9 and BMP15 varied inversely in women with PCO reflecting an aberrant endocrine environment. An increased GDF9:BMP15 ratio may be a new biomarker for PCO.
Inhibin B is a glycoprotein hormone produced mainly by Sertoli cells of the testes in the adult male. It selectively suppresses the secretion of pituitary follicle-stimulating hormone (FSH) and has local paracrine actions in the testes. Its measurement is useful for investigating the role of inhibin B in male gonadal dysfunction. The objective of this study was to investigate the efficacy of serum inhibin B in men with nonobstructive azoospermia in comparison with FSH. Serum concentration of FSH was measured using microparticle enzyme immunoassay, inhibin B by specific solid phase sandwich enzyme-linked immunosorbent assay in men with nonobstructive azoospermia (n = 46) and control fertile men (n = 5). Mean inhibin B and FSH level was 104.6 pg ml−1 and 4.0 mIU ml−1 in control men whereas the value for nonobstructive azoospermic men was 17.06 pg ml−1 and 31.1 mIU ml−1 respectively. Inhibin B and FSH levels were significantly different in azoospermia than controls (P < 0.0001). There were six cases of nonobstructive azoospermia with normal inhibin B. Testicular histology did not find any evidence of spermatogenesis in three cases with normal inhibin B. This demonstrated that inhibin B was not a superior predictor for testicular function in our study.
Education has been started to be stored in the computer away from our minds. : It is aimed through the paper to specify the need of University in correct context through the case study of Madan Bhandari Memorial Academy Nepal. It is conducted using content analysis through consultation. It has worked on 7 projects out of 8 projects within the year 2021-2022. Most of the projects were under Dr. A.K. Mishra who was key scientist. Excessive pressure to attain regular class. Regular absenteeism of teachers was common issue. The management found guilty for hiding weakness of MBMAN by asking to do self-library study, unit test and group study in absence of teacher. This university will be solution for developing sustainable economy focusing self-employment, employee in the nation overcoming high foreign deployment and it will also promote local technology and skills covering school drop outs at various levels. Research based performance evaluation indicator should be introduced in Teachers performance evaluation as proposed. Regularity of faculty should be assured.
Anti-Müllerian Hormone (AMH) is a quantitative marker for ovarian reserve and is used to predict response during ovarian stimulation. Streamlining testing to the clinic or even to the physician's office would reduce inconvenience, turnaround time, patient stress and potentially also the total cost of testing, allowing for more frequent monitoring. In this paper, AMH is used as a model biomarker to describe the rational development and optimization of sensitive, quantitative, clinic-based rapid diagnostic tests.
This project is a study of the politics of mass literacy in India. It sets the problem of literacy in the context of public discourses, institutional practices and formation of various identities related to educational and development goals in contemporary Indian society. The study is centered around specific case studies of two villages under the 'Total Literacy Campaign' (TLC) launched nationwide in May 1988 by the National Literacy Mission Authority (NLMA). Despite marginal improvement in literacy rates since independence, there has been a steady increase in the absolute number of total 'illiterates', which is bigger than the size of 'literates'. Also the gap between 'literates' and 'illiterates' has been ever- increasing. The government admits its failures towards mass education and hence now it has declared promotion of literacy as a national mission. To counter its past failures, it has launched a 'total campaign' approach in adult literacy programme along with 'Education For All' (EFA) goal in general towards elementary education. Thus this project is a study of the pedagogic principles, practice and public policy on promotion mass education and literacy in India. It seeks to study the cultural and linguistic bases of mass literacy and the democratic i.e. participatory and interactive/discursive methods of literacy promotion. The study is based on new socio-cultural approaches to language, discourse-learning, identity and culture (Introduction, Chapter 1). It is an interdisciplinary study in literacy practices (language and discourse learning) whose development is traced with colonial history of social, political and educational development in north India (Chapters 2&3). It traces these developments beginning with nationalist thinking on nation-building, mass literacy and education during the freedom movement before 1947 (Chapter 2), and the evolution of public policy goals on education and its culture after 1947 (Chapter 3). It looks into why highest 'illiteracy' exists in the Hindi- speaking states and how adequately our educational planners have addressed these complex problems. The policy principles and institutional practices in learning are further examined in case studies of two villages in Bihar and Haryana (Chapters 4&5). TLC's pedagogic principles, practices and relevance as seen and understood by learners are examined in order to establish the real contexts of a learning. This is also done through a concrete area of language and content analysis of the TLC reading materials, the way TLC Primers (texts) have been presented to the learners and the ways these texts are interacted with, by the adult learners (Chapter 6). We thus endeavour to establish the issue of literacy-leaming in terms of survival, cultural and identity needs of the learner. All these discussions are primarily based on adult learners' and concerned people's historical, social, and classroom experiences.
Objective: To report a case with distinct difference between the ovarian reserve parameters anti-Mullerian hormone (AMH) and antral follicle courn (AFC) and FSH caused by a novel homozygous missense variant in the exon 1 of the AMH gene [NM_000479.4:c259G>A,p.(Val87Met)].Design: Case report.Setting: Tertiary referral in vitro fertilization clinic.Patient(s): 33-Year-old woman, G4P4A0E0L4, BMI 25.33 Kg/m 2 , with high AFC and repeated extremely low systemic AMH levels detected, measured with multiple ELISA assays.Intervention(s): AMH analysis with multiple assays, Whole exome sequencing (WES) through Next Generation Sequencing (NGS) to diagnose the missense variant, and inhibin B measurement.Main Outcomes Measure(s): Genetic counselling and two subsequent ovarian stimulations for successful fertility preservation.Result(s): Detection of the [NM_000479.4:c259G>A,p.(Val87Met)] variant in the AMH gene.Retrieval and cryopreservation of 4 euploid blastocysts and 26 metaphase-II (MII) oocytes. Conclusion(s):AMH gene mutations can lead to absence of systemic AMH levels and might be discordant to other ovarian reserve markers like AFC, FSH and Inhibin B, without affecting ovarian response to ovarian stimulation.Clinicians should not exclusively rely on AMH for ovarian stimulation.When severely reduced AMH levels are found in patients with high AFC, AMH variants should be suspected, and fertility treatments adequately tailored.
