José Pintado

Institute of Marine Research

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Javier Cremades

Universidade da Coruña

Ingrid Masaló Llorà

Universitat Politècnica de Catalunya

Miquel Planas

Instituto de Investigacións Mariñas

Patrícia Jiménez de Ridder

Universitat Politècnica de Catalunya

Joan Oca Baradad

Universitat Politècnica de Catalunya

Patricia Ruiz

Institute of Marine Research

José Luís Balcázar

Catalan Institute for Water Research

Alexey Vorobev

Inserm

Enora Briand

Ifremer

François H. Lallier

Station Biologique de Roscoff

Cooperative Institutions

Consejo Superior de Investigaciones Científicas

Instituto de Investigacións Mariñas

Centre National de la Recherche Scientifique

Institut de Ciències del Mar

National Research Council

Universitat Politècnica de Catalunya

Institut Català de Ciències del Clima

Institute of Marine Research

Universidade de Vigo

Institut de Recherche pour le Développement

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CONFERENCE SUR LES FONDATIONS

José Pintado

LE CAHIER DE PRESCRIPTIONS TECHNIQUES GENERALES POUR OUVRAGES ROUTIERS ET PONTS REMPLACE CELUI DE 1965 ET REGLEMENTE, DANS SA TROISIEME PARTIE, LA QUESTION DES FONDATIONS. SUR CE POINT, ON A SUPPRIME QUELQUES UNITES DE TRAVAUX (TRANSPORT ADDITIONNEL ET COLMATAGES LOCALISES DE MATERIAU FILTRANT) QUI FIGURAIENT AUPARAVANT, TOUT EN EN RAJOUTANT D'AUTRES. LES CHAPITRES ET UNITES D'OUVRAGES CONSIDEREES SONT: 1) TRAVAUX PRELIMINAIRES: DEFRICHEMENT DU TERRAIN, DEMOLITIONS, SCARIFICATIONS ET COMPACTAGES DU TERRAIN ET DU REVETEMENT EXISTANT, ET ESSAI AVEC SUPERCOMPACTEUR. 2) EXCAVATIONS: DES FONDATIONS ET DES ZONES D'APPOINT, DES FOSSES ET PUITS, EXCAVATIONS SPECIALES DES TALUS EN ROCHE. 3) REMBLAYAGES: REMBLAIS DE TERRE ET DE PIERRE ET COLMATAGES LOCALISES. 4) PHASE FINALE : ACHEVEMENT ET FINITION DU SOL DE FONDATION ET FINITION DES TALUS. ON INDIQUE, POUR CHAQUE UNITE DE TRAVAUX, LA DEFINITION, LA METHODE D'EXECUTION, LES EVENTUELLES OPERATIONS AUXILIAIRES, LA METHODE DE MESURE ET REGLEMENT ET LES QUESTIONS QUE DOIVENT RESOUDRE LE CAHIER DE PRESCRIPTIONS TECHNIQUES PARTICULIERES DU PROJET ET/OU LE DIRECTEUR DE L'OUVRAGE. VOIR FICHE GENERALE DIRR 108595 ET FICHES DETAILLEES 108596 A 108605.

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Vibrio inhibens sp. nov., a novel bacterium with inhibitory activity against Vibrio species

The Journal of Antibiotics (2012)

José Luís Balcázar Miquel Planas José Pintado

Strain (injury)

10.1038/ja.2012.22

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Citations (13)

Challenge 9: Towards an Ocean-Engaged Society

José Pintado Ana Crespo Solana Eva Balsa‐Canto Elisa Berdalet Salvador Bernabéu Albert

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Temperature-induced changes of growth and survival in the early development of the seahorse Hippocampus guttulatus

Journal of Experimental Marine Biology and Ecology (2012)

Miquel Planas Andreu Blanco Alexandro Chamorro Sonia Valladares José Pintado

Seahorse

Gompertz function

10.1016/j.jembe.2012.10.003

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Citations (43)

Isolation and characterization of infectious <i>Vibrio sinaloensis</i> strains from the Pacific shrimp <i>Litopenaeus vannamei</i> (Decapoda: Penaeidae)

Revista de Biología Tropical (2012)

Ma. del Carmen Flores-Miranda Antonio Luna‐González Ángel I. Campa‐Córdova Jesús A. Fierro-Coronado Blanca O. Partida-Arangure

Infectious diseases especially those caused by bacterial and viral pathogens are serious loss factors in shrimp farming. In this study, bacteria were isolated from the gut and hepatopancreas of stressed shrimps obtained from a commercial farm. The isolates were screened on Thiosulfate citrate bile salt sucrose (TCBS) agar plates for the selection of Vibrio species. Presumptive vibrios were characterized through tests for hemolytic and enzymatic activity, hydrophobicity, growth and molecular identification. Three experimental infections were conducted in order to confirm the pathogenicity of selected bacterial strains VHPC18, VHPC23, VHPC24 and VIC30. In the third experimental challenge the LD50 was obtained, it lasted 10 days with 10 shrimp, weighing 6.9±1.1g, per tank. The treatments in triplicate were: (1) saline solution (control group); (2) 2×105CFU/shrimp; (3) 4×105CFU/shrimp; (4) 2×106CFU/shrimp; (5) 4×106CFU/shrimp, and (6) 8×106CFU/shrimp. In all challenges, water parameters measured during the experimental period remained within optimum ranges. Pathogenicity tests confirmed that the mixture of four vibrio isolates, identified as Vibrio sinaloensis, was virulent for L. vannamei. The LD50 value was 1.178×105CFU/g body weight. V. sinaloensis may act as opportunistic pathogens for cultured L. vannamei.

Litopenaeus

Hepatopancreas

Penaeidae

Shrimp farming

Vibrio alginolyticus

Penaeus

10.15517/rbt.v60i2.3914

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Monitoring of the bioencapsulation of a probiotic Phaeobacter strain in the rotifer Brachionus plicatilis using denaturing gradient gel electrophoresis

Aquaculture (2010)

José Pintado María Pérez‐Lorenzo Antonio Luna‐González Cármen G. Sotelo María Jesús Prol

Isochrysis galbana

Brachionus

10.1016/j.aquaculture.2010.02.014

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Citations (29)

Analysis of the diet of the long-snouted seahorseHippocampus guttulatusby 18SrDNA amplification of prey in faeces

Aquaculture Nutrition (2014)

Emmanuel Corse Sonia Valladares Miquel Planas Alexandro Chamorro José Pintado

Breeding in captivity for research or exhibition (e.g. in aquaria) can replace the capture of wild specimens of endangered species and allow controlled reinforcement of wild populations. With this aim, diet analysis and establishing the adequate prey are determinant factors for breeding success. However, non-invasive approaches such as faecal DNA analysis are advisable for analysing the diet of these species. Therefore, the aim of the present study was to demonstrate the usefulness of faecal DNA analysis by specific PCR amplification of prey DNA for assessing the diet of the seahorse Hippocampus guttulatus. In a comparison of the suitability of different genes (COI, 18SrRNA and 28SrRNA), 18SrRNA was found to be the most suitable for designing specific primers for the prey types fed to seahorses (Artemia, Palaemonetes and Mysidae). The technique was assessed in feeding experiments in which prey ingestion was recorded daily, and faeces were collected for DNA extraction and presence/absence PCR analysis. Amplification of the prey DNA in faeces was consistent with the sequence of prey supplied (prey eaten the day before was always detected). Differences in the time between feeding and detection in faeces suggested prey-specific gut passage times, which were shorter for Palaemonetes than for Mysidae. This fact highlights the importance of feeding trials to avoid overestimating the consumption of prey with long gut retention when PCR-based methods are used. This molecular technique is thus applicable for studying the feeding behaviour of captive seahorses and could be adapted for use in other marine species.

Seahorse

Captivity

10.1111/anu.12189

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Citations (28)

A model for experimental infections with Vibrio (Listonella) anguillarum in first feeding turbot (Scophthalmus maximus L.) larvae under hatchery conditions

Aquaculture (2005)

Miquel Planas María Pérez‐Lorenzo José Antonio Vázquez José Pintado

Scophthalmus

Vibrio anguillarum

Hatchery

10.1016/j.aquaculture.2005.04.050

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Citations (39)

New Strategies for the Control of Bacterial Infections in Marine Fish Larval Rearing

Science Publishers eBooks (2011)

José Pintado María Jesús Prol José Luís Balcázar Miquel Planas Pavlos Makridis

One of the limiting steps of marine fi sh aquaculture expansion is the supply of juvenile fi sh. Intensive fi sh larvae production is highly susceptible to the proliferation of bacteria, which may cause poor growth or mass mortality of the larvae. In most cases, mortality cannot be attributed to specifi c pathogens, but to the proliferation of opportunistic bacteria (Olafsen, 1993, 2001). The rearing environment, with high larval densities and high load of organic matter (from faeces, dead larvae, debris, or from live feed) is highly susceptible to bacterial growth. The control of bacteria in rearing systems and live feed production is an important factor for the survival of fi sh larvae (Planas and Cunha, 1999; Skjermo and Vadstein, 1999; Dhert et al., 2001) and the increase in survival of larvae treated with antibiotics supports this fact (Gatesoupe, 1982, 1989; Perez-Benavente and Gatesoupe, 1988). In aquatic environments, the use of antibiotics induces the development of resistances (Sarter and Guichard, 2009, Volume 1 of this Series), which can be transferred to other bacteria, including pathogenic bacteria to fi sh or even humans (Cabello, 2006; Sarter et al., 2007). Therefore, the use of antibiotics should be kept to a minimum. Vaccination of juveniles has

Marine fish

Ichthyoplankton

Fish larvae

10.1201/b10923-2

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Integrated fish-seaweed cultivation in recirculating aquaculture systems: objectives and preliminary results of "IntegRAS" project

Joan Oca Baradad José Pintado Javier Cremades Ingrid Masaló Llorà Patricia Ruiz

Recirculating aquaculture systems (RAS) allow minimizing the water renewal flow rate needed in fish production by increased Nitrogen (NO3 ) and Phosphorous concentrations. Integrating seaweed production to RAS can improve its performances and reduce soluble wastes, contributing to a more sustainable production system.

Recirculating aquaculture system

Sustainable Production

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