Objective To study the diagnosis effect of liquid based cytology combined with colposcopy in cervical precancerous lesions and its clinical application value.Methods Patients with cervical intraepithelial neoplasia were retrospectively studied and divided into observation group given liquid based cytology combined with colposcopy and the control group given colposcopy.Then precancerous lesion classification,quality of life and negative emotions were observed.Results Precancerous lesion classification of observation group is better than that of control group,HAMA and VAS score were significantly lower than that in the control group,MUNSH and QOLS score were significantly higher than that of control group.Conclusion Liquid-based cytology combined with colposcopy which have positive clinical value can diagnose cervical precancerous lesions,improve quality of life and negative emotions.
To compare the effects of moxibustion combined with highly active antiretroviral therapy (HAART) and simple HAART for human immunodeficiency virus (HIV) infected patients.A total of 100 patients with HIV receiving HAART were randomized into an observation group and a control group, 50 cases in each one. In the observation group, moxibustion was used at Zusanli (ST 36), Guanyuan (CV 4) and Sanyinjiao (SP 6), etc. combined with HAART of zidovudine, lamivudine, nevirapine and efavirenzone, etc. Simple HAART was used in the control group. The patients were observed for 18 months. The indexes were CD4+, CD4+/CD8+, interleukin 2 (IL-2), interleukin 7 (IL-7), the incidence of side effects and the score of quality of life.After treatment, CD4+, CD4+/CD8+, serum IL-2 and the scores of quality of life (physiological, psychological, social relation fields and comprehensive score) increased and serum IL-7 decreased compared with those before treatment in the two groups (P<0.01, P<0.05), with better results except CD4+ in the observation group (P<0.01, P<0.05). The incidences of gastrointestinal side effects and total side effects in the observation group were lower than those in the control group (14% (7/50) vs 32% (16/50), 58% (29/50) vs 80% (40/50), both P<0.05).Moxibustion combined with HAART for HIV infected patients could reduce the incidence of side effects, improve medication compliance, CD4+/CD8+, IL-2, IL-7 and the quality of life.
Objectives
This study summed up an initial experience of a single utility video-assisted thoracoscopic surgery (VATS) lobectomy.
Methods
A total of 71 [male 33, female 38; mean age (55.5 ± 6.9 )years old, range 38-72], who underwent major pulmonary resection (segmentectomy, lobectomy, sleeve lobectomy) through a consecutive VATS using a single utility from Dec. 2009 to May 2015, were included in this study. Lobectomy was performed in most patients (n=38), and sleeve lobectomy was performed in 1 patient, and segmentectomy in 2 patients.
Results
The mean time of the operation, in 71 patients who were completed by two-incisions VATS lobectomy, was [(162.9 ± 56.8)minutes (range, 108-320) minutes]. The utility incision length was [(3.2 ± 0.8)cm (range, 2.5-6.0)cm]. The total number of dissected lymph nodes per patient was [(13.4±4.6)range, 8-21 range]. Conversion to thoracotomy was performed in 2 patients (2.8%) due to bleeding at pulmonary arterial branch. The chest tube was removed on postoperative day [(3.8 ± 3.1)range, 3-19 range], and the mean time of hospitalization was [(9.8 ± 5.6)days, range 7-35 days]. There was no occurrence of major perioperative morbidity and mortality.
Conclusion
In the hands of experienced VATS surgeons, it is safe and feasible to perform VATS lobectomy with a single utility.
Key words:
Single; Video-assisted thoracoscopic surgery (VATS); Lobectomy
Objective
To observe the effect of inhibiting the abnormal activation of cdc2 gene on the coordination of mice with Niemann-Pick disease type C(NPC).
Methods
Recombinant adeno-associated virus( rAAV) encoding cdc2-siRNA was packaged, and then was injected into the cerebellum of 2 weeks old npc-/- mice. Footprint test and vertical screen test were performed to assess the coordination of mice at the age of 8 weeks. Purkinje cells visualized by HE staining in cerebellum were counted, and the phosphorylation of microtubule-associated protein Tau recognized by PHF-1 antibody was detected by immunoblotting technology.
Results
(1) Footprint test showed that the stride length in cdc2-siRNA npc-/- group((4.92±0.31) cm) was markedly longer than that in empty vector npc-/- group((4.05±0.19) cm)(P<0.05). (2)Vertical screen test showed that the latency to turn head upwards or reach the upper edge of the screen in cdc2-siRNA npc-/- group((26.01±1.82) s, (50.93±1.98) s) was significantly shorter than that in the empty vector npc-/- group((31.96±3.47)s, (56.89±2.97)s), respectively(P<0.05 for all comparisons). (3)The number of Purkinje cells in cerebellum was dramatically increased in cdc2-siRNA npc-/- group(11.0±2.5) compared with the empty vector npc-/- group(5.1±2.2)(P<0.05). (4)The relative optical densities of cdc2 and phosphorylated Tau immunoreactive bands in cdc2-siRNA npc-/- group(1.42±0.22, 0.95±0.31)were significantly lower than those in the empty vector npc-/- group(2.11±0.29, 2.61±0.62), respectively(P<0.05 for all comparisons).
Conclusion
Inhibiting the abnormal activation of cdc2 gene can improve the coordination of npc-/- mice by ameliorating Purkinje cell’s loss and reducing the hyperphosphorylation of Tau in cerebellum.
Key words:
Niemann-Pick disease type C; Coordination; Cell division cycle 2; Purkinje cell
In the field of minimally invasive surgery, the two-port laparoscopic surgery is on the rise. This study investigated the safety and efficacy of two-port laparoscopic surgery (TLS) for resecting sigmoid colon and upper rectal cancers compared with conventional laparoscopic surgery (CLS).The clinical data of patients undergoing laparoscopic sigmoid colon cancer and upper rectal cancer resection at the Department of General Surgery of the First Affiliated Hospital of Gannan Medical College between July 2019 and January 2022 were retrospectively collected. Grouped according to different laparoscopic surgery. Based on the inclusion and exclusion criteria,A total of 81 patients were enrolled, of the 25 patients from the TLS group,and of the 56 patients from the CLS group. We mainly compared whether there were statistical differences between the two groups in terms of operative time, intraoperative bleeding, incision length, time to first ambulation, time to first flatus, time to first defecation, postoperative complication rate, and other surgical outcomes.There was no statistical difference between the two groups in terms of baseline clinical characteristics (P > 0.05). In terms of the surgical outcomes, there were statistical differences in the total incision length (TLS: 6.21 ± 0.67 cm, CLS: 8.64 ± 1.08 cm, P < 0.001)), time to first ambulation (TLS: 2.0 ± 0.7 d, CLS:3.1 ± 0.9 d, P < 0.001), time to first flatus (TLS: 2.5 ± 0.8 d, CLS: 3.0 ± 0.8 d, P = 0.028), time to first defecation (TLS: 3.8 ± 1.3 d, CLS: 5.1 ± 2.1 d, P = 0.010), and time for liquid diet (TLS: 4.3 ± 1.4 d, CLS: 5.3 ± 1.9 d, P = 0.021). There was no statistical difference between the two groups in terms of the pathology (P > 0.05).In terms of safety, TLS in sigmoid colon and upper rectal cancer resection is comparable to CLS. However, its incision is smaller and more aesthetic, and it causes lesser trauma than CLS. Additionally, it is also superior to CLS in postoperative recovery.
Objective To investigate the role of neurosurgical decompression in the treatment of neuroblastoma with intraspinal extensions.Methods Between February 2000 and May 2007,10 patients (7 male and 3 female,5 age less than 6 months old,3 between 6 months and 12 months old,2 between 1 and 2 years old) were diagnosed as intraspinal neuroblastoma and underwent surgical resection at Xin-Hua hospital in Shanghai.Results Surgical decompression with laminectomy and removal of tumor were performed in 9 patients.One case was diagnosed in other hospital by biopsy,had treated with two courses of chemotherapy,and then ccme to our hospital for neurosurgical decompression.Nine cases were abandoned treatment after surgery,only one followed the chemotherapy.Seven cases were alive in eight followed-up cases.Among them,tumor regression happened in 5 cases,spontaneous maturation in 1 case,and total disappearance after chemotherapy in 1 case.Conclusions The prognosis of patients with intraspinal neuroblastoma is excellent even without radical operation.These findings suggest the possibility that neurosurgical decompression is associated with favorable prognosis.Based on present knowledge,surgical decompression combined with chemotherapy maybe adequate for patients less than 6 months old with intraspinal neuroblastoma.
Key words:
Neuroblastoma; Spinal cord compression; Tumor regression, Spontaneous
Colloid cysts often arise in the third ventricle. It is quite rare for colloid cysts to arise from lateral ventricles. We describe a case of a patient with colloid cysts in the bilateral ventricles. The patient eventually underwent a surgical operation to alleviate the symptoms. Colloid cysts have the potential to be misdiagnosed in the clinical practice; therefore, magnetic resonance imaging and magnetic resonance spectroscopy would be effective for differential diagnosis.
A 60-year-old patient presented with respiratory distress, after recently being tested COVID-19 positive and was mechanically ventilated for 15 days. After cessation of sedation, he remained in deep comatose state, without any reaction on pain stimuli (Glasgow Coma Score 3). MRI of the brain showed diffuse leukoencephalopathy and multiple (>50) microbleeds. Diffuse COVID-19-associated leukoencephalopathy with microhaemorrhages is associated with a poor prognosis. However, 3 months later, our patient showed a remarkable recovery and was able to walk independently. This case report shows COVID-related leukoencephalopathy and intracerebral microbleeds, even with persistent comatose state, may have a favourable clinical outcome and prolonged treatment should be considered in individual cases.
PURPOSE The purpose of this study was to compare the long-term outcomes of laparoscopic and open sphincter-preserving total mesorectal excision (TME) for low rectal cancer (LRC) using propensity score matching (PSM). METHODS The clinical and follow-up data of 169 patients with LRC who underwent sphincter-preserving TME at our institution between January 2011 and January 2014 were retrospectively analyzed. Patients were divided into laparoscopic and open group based on the surgical approach. PSM including age, sex, body mass index, clinical stage, and American Society of Anesthesiologists score with a 1:1 ratio was subsequently performed. Sixty-eight patients in each group were ultimately included, and short- and long-term outcomes were compared between groups. RESULTS Compared with the open group, the laparoscopic group had less intraoperative blood loss, more rapid postoperative recovery, and lower incidence of 30-day postoperative complications. However, there were no significant differences in severity of postoperative 30-day complications between the two groups. Both groups had no intraoperative or 30-day postoperative mortality. Regarding survival outcome, tumor recurrence rate, tumor recurrence site, 5-year overall survival, and 5-year disease-free survival, there were no significant differences between groups. CONCLUSION Laparoscopic sphincter-preserving TME can achieve long-term outcomes similar to those of open TME for LRC.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)
