Shuo Xu

Shanghai Ninth People's Hospital

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Keming Zhu

Jiangsu University

Yanhua Yang

The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture

Huazhong Ren

China Agricultural University

Wei Cao

Case Western Reserve University

Kaixia Li

Jiangsu University

Sundus Zafar

Agricultural Genomics Institute at Shenzhen

Yaoming Li

Jiangsu University

Xiao‐Li Tan

Jiangsu University

Zheng Wang

Liaoning Academy of Agricultural Sciences

Xingwang Liu

China Agricultural University

Cooperative Institutions

China Agricultural University

Shanghai Jiao Tong University

Chinese Academy of Sciences

Shanghai Ninth People's Hospital

Jiangsu University

University of Chinese Academy of Sciences

Chinese Academy of Agricultural Sciences

Institute of Genetics and Developmental Biology

Shandong Agricultural University

Ministry of Agriculture and Rural Affairs

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Overexpression of salt-induced protein (salT) delays leaf senescence in rice

Figshare (2019)

Keming Zhu Huimin Tao Shuo Xu Kaixia Li Sundus Zafar

Abstract Senescence, a highly programmed process, largely determines yield and quality of crops. However, knowledge about the onset and progression of leaf senescence in crop plants is still limited. Here, we report that salt-induced protein (salT), a new gene, may be involved in leaf senescence. Overexpressing salT could prolong the duration of leaves with higher concentrations of chlorophyll compared with the wild type. Moreover, overexpression of salT could delay the senescence of rice leaves though the inhibition of senescence associated genes (SAGs). Overall, the characterization of salT suggested that it is a new gene affecting the leaf senescence induced by natural and dark conditions.

Senescence

10.6084/m9.figshare.7676558

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Cucumber (Cucumis sativus L.) Nitric Oxide Synthase Associated Gene1 (CsNOA1) Plays a Role in Chilling Stress

Frontiers in Plant Science (2016)

Xingwang Liu Bin Liu Shudan Xue Yanlinq Cai Wenzhu Qi

Nitric oxide (NO) is a gaseous signaling molecule in plants, transducing information as a result of exposure to low temperatures. However, the underlying molecular mechanism linking NO with chilling stress is not well understood. Here, we functionally characterized the cucumber (Cucumis sativus) nitric oxide synthase-associated gene, NITRIC OXIDE ASSOCIATED 1(CsNOA1). Expression analysis of CsNOA1, using quantitative real-time PCR, in situ hybridization and a promoter::β-glucuronidase (GUS) reporter assay, revealed that it is expressed mainly in the root and shoot apical meristem (SAM), and that expression is up-regulated by low temperatures. A CsNOA1-GFP fusion protein was found to be localized to the mitochondria, and ectopic expression of CsNOA1 in the A. thaliana noa1 mutant partially rescued the normal phenotype. Transgenic cucumber plants revealed that the gene is required by seedlings to tolerate chilling stress: constitutive over-expression of CsNOA1 led to a greater accumulation of soluble sugars, starch, and an up-regulation of Cold-regulatory C-repeat binding factor3 (CBF3) expression. Conversely, suppression of CsNOA1 expression resulted in the opposite phenotype and a reduced NO content compared to wild type plants. Additionally, analyses using inhibitors suggested that NO can be synthesized via a CsNOA1 dependent pathway that is independent of the nitrate reductase (NR) pathway.

Cucumis

Ectopic expression

10.3389/fpls.2016.01652

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Citations (23)

Horticulture Research (2024)

Shuo Xu Yaru Wang Songlin Yang Shanshan Fan Kexin Shi

Abstract Cucumber (Cucumis sativus L.) is a widely cultivated crop with rich germplasm resources, holding significant nutritional value. It also serves as an important model for studying epidermal cell fate and sex determination. Cucumbers are covered with multicellular and unbranched trichomes, including a specific type called spines found on the surface of the fruit. The presence and density of these fruit spines determine the visual quality of cucumber fruits. However, the key regulatory genes and mechanisms underlying cucumber fruit spine development remain poorly understood. In this study, we identified a WUSCHEL-related homeobox (WOX) family gene CsWOX3, which functioned as a typical transcriptional repressor and played a negative role in fruit spine development. Spatial-temporal expression analysis revealed that CsWOX3 exhibited a relatively high expression level in the cucumber female floral organs, particularly in the fruit exocarp. Knockout of CsWOX3 using CRISPR-Cas9 resulted in a significant 2-to-3-fold increase in the diameter of fruit spines base, while overexpression led to a 17% decrease in the diameter compared to the wild-type. A SQUAMOSA PROMOTER BINDING PROTEIN-LIKE transcription factor CsSPL15, could directly bind and activate the expression of CsWOX3, thereby suppressing the expression of downstream auxin-related genes, such as CsARF18. Additionally, the RING-finger type E3 ubiquitin ligase CsMIEL1-like interacted with the HD domain of CsWOX3, which might result in the ubiquitination and subsequent alteration in protein stability of CsWOX3. Collectively, our study uncovered a WOX transcription factor CsWOX3 and elucidated its expression pattern and biological function. This discovery enhances our comprehension of the molecular mechanism governing cucumber fruit spine morphogenesis.

Cucumis

10.1093/hr/uhae163

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Citations (2)

LAZY3 interacts with LAZY2 to regulate tiller angle by modulating shoot gravity perception in rice

Plant Biotechnology Journal (2023)

Yueyue Cai Linzhou Huang Yuqi Song Yundong Yuan Shuo Xu

Summary Starch biosynthesis in gravity‐sensing tissues of rice shoot determines the magnitude of rice shoot gravitropism and thus tiller angle. However, the molecular mechanism underlying starch biosynthesis in rice gravity‐sensing tissues is still unclear. We characterized a novel tiller angle gene LAZY3 ( LA3 ) in rice through map‐based cloning. Biochemical, molecular and genetic studies further demonstrated the essential roles of LA3 in gravity perception of rice shoot and tiller angle control. The shoot gravitropism and lateral auxin transport were defective in la3 mutant upon gravistimulation. We showed that LA3 encodes a chloroplast‐localized tryptophan‐rich protein associated with starch granules via Tryptophan‐rich region (TRR) domain. Moreover, LA3 could interact with the starch biosynthesis regulator LA2, determining starch granule formation in shoot gravity‐sensing tissues. LA3 and LA2 negatively regulate tiller angle in the same pathway acting upstream of LA1 to mediate asymmetric distribution of auxin. Our study defined LA3 as an indispensable factor of starch biosynthesis in rice gravity‐sensing tissues that greatly broadens current understanding in the molecular mechanisms underlying the starch granule formation in gravity‐sensing tissues, and provides new insights into the regulatory mechanism of shoot gravitropism and rice tiller angle.

Tiller (botany)

10.1111/pbi.14031

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Citations (14)

Overexpression of salt-induced protein (salT) delays leaf senescence in rice

Figshare (2019)

Keming Zhu Huimin Tao Shuo Xu Kaixia Li Sundus Zafar

Senescence

10.6084/m9.figshare.7866071

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Citations (0)

Environmental beliefs and public acceptance of nuclear energy in China: A moderated mediation analysis

Energy Policy (2019)

Jing Wang Yazhou Li Jianlin Wu Jibao Gu Shuo Xu

Moderated mediation

Social Acceptance

10.1016/j.enpol.2019.111141

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Citations (34)

Proteomic analysis of a clavata-like phenotype mutant in Brassica napus

Genetics and Molecular Biology (2020)

Keming Zhu Weiwei Zhang Rehman Sarwa Shuo Xu Kaixia Li

Rapeseed is one of important oil crops in China. Better understanding of the regulation network of main agronomic traits of rapeseed could improve the yielding of rapeseed. In this study, we obtained an influrescence mutant that showed a fusion phenotype, similar with the Arabidopsis clavata-like phenotype, so we named the mutant as Bnclavata-like (Bnclv-like). Phenotype analysis illustrated that abnormal development of the inflorescence meristem (IM) led to the fused-inflorescence phenotype. At the stage of protein abundance, major regulators in metabolic processes, ROS metabolism, and cytoskeleton formation were seen to be altered in this mutant. These results not only revealed the relationship between biological processes and inflorescence meristem development, but also suggest bioengineering strategies for the improved breeding and production of Brassica napus.

Silique

10.1590/1678-4685-gmb-2019-0305

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Citations (1)

Evolution and Transformation of the Central Place Theory in E-Business: China’s C2C Online Game Marketing

Sustainability (2019)

Li Qiang Qing Liu Xiaona Guo Shuo Xu Jingyu Liu

In contemporary studies in areas such as the field of classical theory, there has been an increasing emphasis on E-Business. In this study, we examine central place theory principles using China’s consumer-to-consumer online game marketing as a case of E-business. The results indicate that virtual goods in China’s C2C online game marketing are distributed in areas with high economic development and where residents have high purchasing power. Further, we found that the transmission capacity of virtual goods is affected by the level of telecommunication services because they are transmitted through information flow. Such effects illustrate that virtual goods are no longer affected by spatial distance and traffic conditions because virtual goods do not need to be touched; however, they are still affected by market forces. We conclude that there are key differences between the marketing and transport principles of CPT based on the hierarchical model describing new internal and external relations and the level of complexity in E-Business processes.

10.3390/su11082274

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Citations (6)

A CBL4-CIPK6 module confers salt tolerance in cucumber

Vegetable Research (2022)

Mingfei Wang Songlin Yang Lei Sun Zhongxuan Feng Yiming Gao

Soil salinization is a major threat to cucumbers grown under protected cultivation. Under stressful environments, calcineurin B-like proteins (CBLs) can sense and bind Ca²⁺ signals and regulate CBL-interacting protein kinases (CIPKs) to transmit signals and induce cellular responses. Although CBL-CIPK modules play central roles in plant development and response to various abiotic stresses in Arabidopsis, little is known about their functions in cucumber. In this study, we demonstrate that CsCBL4 interacts with CsCIPK6, which exhibited similar responses to salt stress in cucumber. Furthermore, salt stress resulted in greater accumulation of CsCBL4 and CsCIPK6. Comprehensive phenotype analysis demonstrated that silencing CsCBL4 or CsCIPK6 reduced the salt tolerance of cucumber, and overexpression of CsCBL4 increased the salt tolerance of Arabidopsis. Collectively, these results indicate that the CsCBL4-CsCIPK6 module plays an important role in the resistance of cucumber to salt stress. The information provides insights for the genetic breeding of salt tolerance in cucumber in the future.

Cucumis

10.48130/vr-2022-0007

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Citations (7)

Analyses of a Glycine max Degradome Library Identify microRNA Targets and MicroRNAs that Trigger Secondary SiRNA Biogenesis

Journal of Integrative Plant Biology (2012)

Zheng Hu Qiyan Jiang Zhiyong Ni Rui Chen Shuo Xu

Plant microRNAs (miRNAs) regulate gene expression mainly by guiding cleavage of target mRNAs. In this study, a degradome library constructed from different soybean (Glycine max (L.) Merr.) tissues was deep-sequenced. 428 potential targets of small interfering RNAs and 25 novel miRNA families were identified. A total of 211 potential miRNA targets, including 174 conserved miRNA targets and 37 soybean-specific miRNA targets, were identified. Among them, 121 targets were first discovered in soybean. The signature distribution of soybean primary miRNAs (pri-miRNAs) showed that most pri-miRNAs had the characteristic pattern of Dicer processing. The biogenesis of TAS3 small interfering RNAs (siRNAs) was conserved in soybean, and nine Auxin Response Factors were identified as TAS3 siRNA targets. Twenty-three miRNA targets produced secondary small interfering RNAs (siRNAs) in soybean. These targets were guided by five miRNAs: gma-miR393, gma-miR1508, gma-miR1510, gma-miR1514, and novel-11. Multiple targets of these secondary siRNAs were detected. These 23 miRNA targets may be the putative novel TAS genes in soybean. Global identification of miRNA targets and potential novel TAS genes will contribute to research on the functions of miRNAs in soybean.

Dicer

Drosha

Trans-acting siRNA

10.1111/jipb.12002

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Citations (24)