Adeline Andrade Carvalho

Universidade Federal do Piauí

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Cleidson Manoel Gomes da Silva

Universidade Federal do Sul e Sudeste do Pará

J.R. Figueiredo

Universidade Estadual do Ceará

Luciana Rocha Faustino

Universidade Federal do Piauí

C.C. Campello

Universidade Estadual do Ceará

Ana Paula Ribeiro Rodrigues

Universidade Estadual do Ceará

Simone Vieira Castro

Universidade do Tocantins

C.A.P. Lopes

Universidade de Brasília

Sônia Nair Báo

Universidade de Brasília

Amilton Paulo Raposo Costa

Universidade Federal do Piauí

Francielli Weber Santos Cibin

Universidade Federal do Pampa

Cooperative Institutions

Universidade Estadual do Ceará

Universidade Federal do Ceará

Universidade Federal do Piauí

Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia

Universidade de Fortaleza

Universidade Federal de Minas Gerais

Universidade Federal de Santa Maria

Universidade de Brasília

Universidade Federal de São Paulo

Universidade Federal do Maranhão

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Influence of vitrification techniques and solutions on the morphology and survival of preantral follicles after in vitro culture of caprine ovarian tissue

Theriogenology (2011)

Adeline Andrade Carvalho Luciana Rocha Faustino Cleidson Manoel Gomes da Silva Simone Vieira Castro Hiédely Kenia Machado Luz

Vitrification

Ovarian Cortex

Trypan blue

Histology

10.1016/j.theriogenology.2011.04.024

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Citations (47)

Immunolocalization of angiotensin-(1-7), angiotensin II and angiotensin-converting enzyme 2 in goat ovary

Acta Veterinaria Brasilica (2021)

Lauro César Soares Feitosa Alécio Matos Pereira Adeline Andrade Carvalho Antônio de Sousa Júnior Kinulpe Honorato‐Sampaio

There is increasing evidence as to the participation of the ovarian renin-angiotensin system in important reproductive processes. The inhibition of the angiotensin-converting enzyme (ACE) caused an increase in the rate of ovulation and pregnancy in the artificial insemination protocol has fixed time (TFIA). This study aimed to investigate the presence and location of Ang II, Ang- (1-7) and ACE2 in goat ovaries and the possibility of the involvement of these peptides in previous results. Ten ovaries from goats were collected in a slaughterhouse, washed in buffered PBS, perfused with protease inhibitor solution and processed for immunohistochemistry protocol. The search for peptides was performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cells of antral follicles and corpus luteum was observed. Antral follicles (theca cells), corpus luteum and oocyte cytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strong immunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. In this study, for the first time, the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, suggesting that there is participation in follicular development, oocyte maturation and corpus luteum development.

Antral follicle

10.21708/avb.2021.15.3.9629

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Citations (0)

Novel wide-capacity method for vitrification of caprine ovaries: Ovarian Tissue Cryosystem (OTC)

Animal Reproduction Science (2013)

Adeline Andrade Carvalho Luciana Rocha Faustino Cleidson Manoel Gomes da Silva Simone Vieira Castro C.A.P. Lopes

Vitrification

10.1016/j.anireprosci.2013.02.015

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Citations (52)

Catalase Prevents Lipid Peroxidation and Enhances Survival of Caprine Preantral Follicles Cryopreserved in a 1,2-Propanediol-Freezing Medium

Biopreservation and Biobanking (2012)

Hiédely Kenia Machado Luz Regiane R. Santos Livia Schell Wanderley Luciana Rocha Faustino Cleidson Manoel Gomes da Silva

The objectives of this study were to determine: 1) the optimal concentration (1.0 or 1.5 M) and duration of exposure (5, 10, or 20 min) of ovarian tissue to 1,2-propanediol (PROH) on morphology and viability of caprine preantral follicles; and 2) the effect of supplementing cryopreservation medium supplementation with Trolox® (0.1, 0.5, or 1.0 mM) or catalase (5, 10, or 20 IU/mL) on follicular morphology, viability, and lipid peroxidation. Cryopreservation decreased (p<0.05) percentages of normal follicles relative to the control (84%). Although supplementation of the cryopreservation medium (1.0 M PROH) with catalase (10 or 20 IU/mL) or Trolox® (0.1 mM) resulted in follicular morphology and viability similar to that in the controls (P>0.05), lipid peroxidation was reduced only when 20 IU/mL catalase was added to the cryopreservation medium.

Propanediol

10.1089/bio.2011.0046

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Citations (18)

Freezing solution containing dimethylsulfoxide and fetal calf serum maintains survival and ultrastructure of goat preantral follicles after cryopreservation and in vitro culture of ovarian tissue

Cell and Tissue Research (2011)

Simone Vieira Castro Adeline Andrade Carvalho Cleidson Manoel Gomes da Silva Luciana Rocha Faustino C.C. Campello

Cryoprotectant

Fetal bovine serum

Ovarian Cortex

In vitro maturation

10.1007/s00441-011-1257-8

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Citations (24)

Frozen and Fresh Ovarian Tissue Require Different Culture Media to Promote in Vitro Development of Bovine Preantral Follicles

Biopreservation and Biobanking (2014)

Simone Vieira Castro Adeline Andrade Carvalho Cleidson Manoel Gomes da Silva Francielli Weber Santos Cibin C.C. Campello

The aim of this study was to evaluate the efficiency of different media in the in vitro culture of bovine preantral follicles that were used either fresh or following slow freezing treatment. Frozen and fresh noncultured or cultured ovarian fragments were processed for histological, viability, and cell proliferation analyses. For cryopreservation, a solution containing 1.5 M ethylene glycol was frozen in a programmable biological freezer. After thawing, a portion of the samples was destined for frozen controls. The remainder were cultured in vitro for 5 days in three media: α-MEM, McCoy, or M199. Samples from these culture media were collected on days 1 and 5 for quantification of reactive oxygen species (ROS) and for hormonal assays. In fresh-cultured tissues, the percentage of morphologically normal follicles was significantly higher when cultured in M199 compared to that in the other media. In frozen-cultured tissues, McCoy medium was significantly superior to the other media, and was the only treatment that helped in maintaining the viability similar to fresh and frozen controls. Upon quantification of the nucleolus organizer region, we observed greater proliferation of granulosa cells in the frozen-cultured tissues with McCoy medium, and lesser proliferation in fresh-cultured tissues only with α-MEM. In frozen-cultured tissues, ROS levels were highest at day 1 and progressively reduced during culture, independent of the media used. In conclusion, under the conditions used in this study, the M199 and McCoy media are recommended for the culture of follicles derived from fresh and frozen ovarian tissues, respectively.

10.1089/bio.2014.0020

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Citations (18)

Hémangiomatose sclerosante atypique. Considérations sur un cas particulier, voisin du syndrome de Maffucci.

Archives francaises de pédiatrie (1969)

Bruna Schmidt de Tomasi Calil K. Farhat Joser Cleyton Neves S Cavalhal

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Assessment of DNA damage in goat preantral follicles after vitrification of the ovarian cortex

Reproduction Fertility and Development (2014)

Luciana Rocha Faustino Adeline Andrade Carvalho Cleidson Manoel Gomes da Silva R. Rossetto C.A.P. Lopes

Effective methods for gamete preservation should have low impact on DNA integrity. The present study investigated the effects of vitrification of goat ovarian tissues on the occurrence of DNA fragmentation and DNA double-stand breaks using the terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL) assay and detection of phosphorylated histone H2AX (γH2AX), respectively. Goat ovaries were collected at a local abattoir and 12 tissue fragments were prepared from each ovarian pair. Tissue fragments were used as fresh control samples or were cultured in vitro, vitrified or vitrified and cultured. Vitrification was performed using the Ovarian Tissue Cryosystem. Fragments from all groups (control and treatments) were processed for histology, transmission electron microscopy, TUNEL assay and immunofluorescence. Compared with fresh control samples, a lower percentage of morphologically normal follicles was detected in the vitrification followed by culture treatment group (P<0.05). Normal follicular ultrastructure was observed in all groups. Immunofluorescence revealed the presence of γH2AX foci in few oocytes and ovarian stromal cells. TUNEL-positive follicles were found in samples without significant differences among groups (P>0.05). In conclusion, the vitrification protocol used in the present study did not increase DNA damage in preantral follicles enclosed in goat ovarian tissues.

Vitrification

Ovarian Cortex

Fragmentation

10.1071/rd13164

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Citations (10)