Objective To investigate the influence of laparoscopic resection of colorectal cancer on (postoperative) micrometastasis of tumor cells.Methods Using cytokeratin,CK-20 mRNA as target gene,the RT-PCR method was used to examine the pre-and post-operative changes in the number of tumor cells in the peripheral blood of 48 cases of colorectal cancer.Results After operation,the number of tumor cells in(peripheral) blood was significantly higher in the laparoscopic surgery group as compared to that of traditional(laparotomy) operation group(P0.05).The degree of increase in the number of tumor cells in peripheral blood after laparoscopic surgery was not related to patient′s age(P0.05),but was significantly related to positive rate of proliferating cell nuclear antigen(PCNA)(P0.05).Conclusions Laparoscopic resection of colorectal cancer may increase the risk of hematogenous tumor metastasis,and this risk is ever higher in(patients) with PCNA≥50% who undergo laparoscopic resection of colorectal cancer.
Gastric cancer (GC) remains one of the predominant malignant tumors within the digestive tract, yet its underlying biological mechanisms remain elusive. The primary objective of this study is to delineate the causal relationship between circulating metabolites and GC.
This study aimed to explore the application values of modified radical mastectomy in female patients with mammary cancer of different molecular types and from this we conducted a prognosis study.A total of 204 Breast Cancer (BC) patients who were admitted to our hospital from March 2015 to March 2017 were included and divided into Group A (Luminal A type, n = 68), Group B (Luminal B type, n = 48), Group C (ERBB2: Erb-B2 Receptor Tyrosine Kinase 2 + type, n = 42), and Group D (Basal-like type, n = 46) according to their molecular cancer types. Patients in Groups A and B demonstrated superior treatment efficacy and lower incidence of adverse reactions than those in Groups C and D (P < 0.05), while no statistical difference was observed among the 4 groups in terms of the total operation time, intraoperative blood loss, and postoperative 48-h drainage volume (P > 0.05). Before treatment, the 4 groups exhibited similar results from the EORTC breast cancer-specific quality of life questionnaire (EORTCQLQ-BR23) (P > 0.05).After treatment, Group A was superior to the other 3 groups in this regard (P < 0.05). Further, no significant difference was observed among the 4 groups in terms of the prognosis of 3-year survival (P > 0.05).The clinical application of modified radical mastectomy does not depend on the molecular typing of BC; however, the treatment was more effective in the treatment of Luminal A type BC.
Background: Nowadays, more and more critical neonatal and pediatric patients accepted extracorporeal membrane oxygenation (ECMO) therapy in China. We analyzed the incidence, etiology, and impact of NIs in severe patients supported by ECMO from seven pediatric intensive care units (PICUs) in China.Methods: This retrospective, multicenter study included participants with laboratory-detected nosocomial infections who were admitted to seven PICUs in China from September 2012 to February 2020. We conducted a retrospective chart review of 322 neonatal and pediatric patients. Clinical NI data were collected and analyzed retrospectively. We used univariable analysis to identify factors associated with risk of NIs and death.Findings: 322 neonatal and pediatric patients supported by extracorporeal membrane oxygenation (93·17% veno-arterial) for medical indications (36·96% acute respiratory distress syndrome) were included in the analysis. 50 cases of ventilator-associated pneumonia (VAP), five cases of urinary tract infections (UTI), 27 cases of blood stream infections (BSI) and six cases of catheter-related blood stream infections (CRBSI) were identified, totally 88 nosocomial infections (27·33%). There were much more G- infections compared with G+ (84·09% vs 6·82%; P <0·01). 83·75% infections were caused by multidrug-resistant organisms. Older age (29 days–16 years old) and complications with EMCO were independently associated with higher risk of nosocomial infections. The most common infections were VAP caused by G-, in total 50 infections (56·82%). 169 patients (52·48%) died (70·06 deaths / 1,000 person- days of extracorporeal membrane oxygenation). Infected patients had higher mortality (65·50% vs. 48·72%, P <0·05), longer hospital stays (39·62[4·00-129·00] vs. 26·04[1·67-140·00] days; P <0·001), ICU length of stay (28·49[4·00-125·00] vs. 16·93[0·50-94·70] days; P <0·001), invasive mechanical ventilation (IMV) duration (22·43[3·00-124·00] vs. 12·24[1·00-79·83] days; P <0·001) and ECMO duration (11·01[1·00-59·53] vs 6·17[1·00-79·66] days; P <0·001). The vasoactive drug index both before ECMO were independently associated with higher risk for death.Interpretation: Nosocomial infections in neonatal and pediatric patients supported by ECMO are usually involved with multidrug resistant bacteria. It also increases risk of patient’s mortality, PICU days, IMV days and ECMO days. Complications during EMCO increases infection ratio, which also serves as an independent risk factor correlating with the first NI and mortality significantly. Therefore, complications during ECMO should be vigorously monitored and minimized in clinical practice.Funding: This study was supported by Capital’s Funds for Health Improvement and Research (2020-2-5093)Declaration of Interests: The authors declare no competing interests.Ethics Approval Statement: This study was approved by Ethics committee.
OBJECTIVE To evaluate the role of targeted adsorption of miR-218 by long-chain non-coding RNAHOTAIR to regulate PDE7A on glioma cell proliferation, invasion, and apoptosis. METHODS The expressions of lncRNA HOTAIR, miR-218, and PDE7A in glioma tissues and normal parcancer tissues, NHA and glioma cell lines were determined, and correlations among the three genes were analyzed. The subcellular localization of lncRNA HOTAIR was determined by fluorescent in situ hybridization. Dual-luciferase reporter assay was used to validate the targeted relationship between lncRNA HOTAIR/miR-218/PDE7A. Glioma cells were grouped to receive intervention of lncRNA HOTAIR or miR-218. MTT, transwell, and flow cytometry were performed to determine the proliferation, invasion, and apoptosis of cells. RESULTS Compared with the normal tissues and cells, the expression of lncRNA HOTAIR was increased while miR-218 was suppressed in glioma tissues samples and cells (all P<0.05). Inhibition of lncRNA HOTAIR expression, was able to induce apoptosis and suppress the proliferation and invasion of cells (all P<0.05). LncRNA HOTAIR is mainly localized in the cytoplasm, and is able to adsorb miR-218 as ceRNA. The effect of knockdown of HOTAIR on glioma cells could be partially rescued by miR-218 inhibitor. The expression of PDE7A was enhanced in glioma tissues and cells compared to normal tissues and cells (all P<0.05), which positively correlated with the expression of HOTAIR (r=0.546, P<0.05) and negatively correlated with the expression of miR-218 (r=0.363, P<0.05). The targeted relationship between miR-218 and PDE7A was validated: Overexpression of miR-218 was able to suppress the proliferation and invasion of glioma cells and restrain apoptosis compared to the miR-NC group (all P<0.05). The effect of miR-218 on glioma cells could be partially rescued by PDE7A. CONCLUSION lncRNA HOTAIR can adsorb miR-218 to regulate expression of PDE7A and promote the malignant biologic behavior of glioma cells.
Objective
To compare the effect of improved exterior route dacryocystorhinostomy and nasal endoscopic dacryocystorhinostomy for postoperatively recurrent chronic dacryocystitis.
Methods
Eighteen eyes of 18 cases with postoperative recurrence of chronic dacryocystitis were randomly divided into two groups. 8 eyes of group A was treated with improved dacryocystorhinostomy, and 10 eyes of group B was treated with improved nasal endoscopic dacryocystorhinostomy. All patients were followed up for 6 months.
Results
In group A, 6 eyes were cured, 1 eyes was improved and 1 eye was noneffective; the total therapeutic effective rate was 87.5%. In group B, 9 eyes were cured, 1 eyes was improved and none was noneffective; the total treatment effective rate was 100%. The difference between two groups was statistically significant(χ2=7.8030, P=0.0019).
Conclusion
Nasal endoscopic dacryocystorhinostomy is suitable for postoperative recurrence chronic dacryocystitis. Interdisciplinary cooperation between ophthalmologist and rhinologist is required.
Key words:
Dacryocystorhinostomy, nasal endoscopic; Dacryocystitis, chronic, recurrent; Cooperation, interdisciplinary, ophthalmology, rhinology
To investigate the expression of cystatin C gene and its effect on the proliferation, apoptosis and invasiveness of EC9706 cells in esophageal carcinoma.56 cases of esophageal carcinoma were randomly collected from our hospital. Samples of human esophageal carcinomas and matched normal esophageal mucosal epithelium were selected by resection operation from these patients. Expression of cathepsin B and cystatin C in these specimens were determined by immunohistochemistry and qRT-PCR. Next, lentiviral vectors of over-expression and interference for cystatin C gene were constructed, and both were transfected into EC9706 cells, and then the levels of cystatin C mRNA and protein were detected by qRT-PCR and Western blot. The effect of over and low-expressed cystatin C on the proliferation, apoptosis and invasiveness of esophageal carcinoma cells were detected by MTT assay, flow cytometry and Transwell assay.Compared with normal esophageal epithelial tissues, mRNA and protein levels of cathepsin B and cystatin C in esophageal carcinoma tissues were significantly increased (P<0.05). Lentiviral vectors of over-expression and interference for cystatin C gene were successfully transfected into EC9706 cells. Over or low-expression cystatin C had no effect on EC9706 cells proliferation but had a reverse relationship with the apoptosis. However, cystatin C over-expression significantly decreased tumor invasiveness (P<0.05) while the invasiveness of EC9706 cells was significantly enhanced by RNAi-mediated abrogation of cystatin C gene expression (P<0.05).Over-expressed cystatin C could inhibit the invasiveness of esophageal carcinoma cells.
Objective
To discuss the related prognostic factors of hypospadias, and the effect of prognostic factors on the success rate in hypospadias surgery.
Methods
The clinical data of 276 cases of hypospadias admitted in our hospital were retrospectively studied, including age, Duckett classification, degree of penile curvature, surgical operations, urethral flap sources, surgical staging, cystostomy,indwelling catheter time, operating time, suture,accompnied diseases,surgeons'experience, et al. Univariate and multivariate, and Logistic regression analyses were done.
Results
Univariate analysis showed that the following features were associated with hypospadias: Duckett classification(t= 7.326, P< 0.05), Donnahoo(t= 6.107, P< 0.05), operative time(t= 3.639, P< 0.05), and surgeons’experience(t= 3.154, P< 0.05).Multivariate analysis showed Duckett classification could be independent predictive factor of hypospadias[odds ratio(OR)= 2.017, P< 0.05].
Conclusion
Duckett classification could be independently predictive factor in the success rate of hypospadias operation.
Key words:
Hypospadias; Prognostic; Risk factors
Extensive research has explored the role of aldosterone in insulin resistance. Recent evidence suggests that the mineralocorticoid receptor (MR) mediates aldosterone-induced dysregulation of cytokines, and most of this research has focused on adjustments in fat tissue and adipocytes. However, the direct effect of MR blockade on insulin resistance in cardiomyocytes remains largely unknown. In the present study, we investigated whether MR blockade improves insulin-sensitizing factors in insulin-resistant rats and attenuates the dysregulation of the aldosterone-related transport of adiponectin and glucose in cardiomyocytes and examined the underlying mechanisms.The effects of aldosterone, MR inhibitors (e.g., eplerenone), a peroxisome proliferator-activated receptor (PPAR) α agonist, and a p38 mitogen-activated protein kinase (MAPK) inhibitor on adiponectin and glucose transport were studied at the mRNA and protein levels in vitro and in vivo.Our data revealed that aldosterone reduced the expression of adiponectin and inhibited the transport of glucose in cardiomyocytes and that MR blockade reversed these affects. In vivo, MR blockade improved insulin-sensitive parameters and increased adiponectin expression in the myocardia of high-fat diet rats. Furthermore, aldosterone promoted p38MAPK expression but negatively affected PPARα expression, and the downregulation of adiponectin by aldosterone was reversed by MR blockade, a PPARα agonist, and a p38 MAPK inhibitor.The above results suggested that aldosterone promoted insulin resistance in the heart and that this effect could be partly reversed by MR blockade through signal transduction in the P38 MAPK pathway and PPARα.
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