Ping Chen

Daping Hospital

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Bei Sun

Central South University

Fengxia Qi

Chinese Academy of Sciences

Page W. Caufield

New York University

Gang Wang

Harbin Medical University

Minghui Wang

Henan University

Ming Hao

Harbin Medical University

Su-rong Li

Institute of Psychology, Chinese Academy of Sciences

Hongchi Jiang

First Affiliated Hospital of Harbin Medical University

Haonan Jiang

Guangzhou Medical University

Tingting Hu

China Agricultural University

Cooperative Institutions

Chinese Academy of Agricultural Sciences

Guangzhou Medical University

Harbin Medical University

Fudan University

First Affiliated Hospital of Harbin Medical University

Army Medical University

First Affiliated Hospital of Guangzhou Medical University

Chinese Academy of Sciences

Jilin University

State Key Laboratory of Respiratory Disease

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Engineered global regulator H-NS improves the acid tolerance of E. coli

Microbial Cell Factories (2018)

Xianxing Gao Xiaofeng Yang Jiahui Li Yan Zhang Ping Chen

Acid stress is often encountered during industrial fermentation as a result of the accumulation of acidic metabolites. Acid stress increases the intracellular acidity and can cause DNA damage and denaturation of essential enzymes, thus leading to a decrease of growth and fermentation yields. Although acid stress can be relieved by addition of a base to the medium, fermentations with acid-tolerant strains are generally considered much more efficient and cost-effective. In this study, the global regulator H-NS was found to have significant influence on the acid tolerance of E. coli. The final OD600 of strains overexpressing H-NS increased by 24% compared to control, when cultured for 24 h at pH 4.5 using HCl as an acid agent. To further improve the acid tolerance, a library of H-NS was constructed by error-prone PCR and subjected to selection. Five mutants that conferred a significant growth advantage compared to the control strain were obtained. The final OD600 of strains harboring the five H-NS mutants was enhanced by 26–53%, and their survival rate was increased by 10- to 100-fold at pH 2.5. Further investigation showed that the improved acid tolerance of H-NS mutants coincides with the activation of multiple acid resistance mechanisms, in particular the glutamate- and glutamine-dependent acid resistance system (AR2). The improved acid tolerance of H-NS mutants was also demonstrated in media acidified by acetic acid and succinic acid, which are common acidic fermentation by-products or products. The results obtained in this work demonstrate that the engineering of H-NS can enhance the acid tolerance of E. coli. More in general, this study shows the potential of the engineering of global regulators acting as repressors, such as H-NS, as a promising method to obtain phenotypes of interest. This approach could expand the spectrum of application of global transcription machinery engineering.

10.1186/s12934-018-0966-z

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Current status and prospects of minimally invasive hepatobiliary surgery

Intelligent Surgery (2024)

Jie Li Ping Chen

In recent years, minimally invasive surgery has developed rapidly and has become an important means of treating hepatobiliary diseases. The development of minimally invasive surgery includes advancements in surgical instruments, laparoscopic equipment, surgical techniques, and related theories. Currently, most traditional hepatobiliary surgeries can be performed using laparoscopic methods. Minimally invasive surgery is the direction for future surgical development, with goals of becoming more intelligent, compact, and multifunctional. In the future, the continuous integration of artificial intelligence, robotic technology, virtual reality and augmented reality technologies, and remote technologies with medicine will further promote the development of minimally invasive surgery. This article provides a brief overview of the current status and prospects of minimally invasive surgery.

Invasive surgery

Minimally invasive procedures

Surgical procedures

10.1016/j.isurg.2024.07.001

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The Specific Genes for Lantibiotic Mutacin II Biosynthesis in Streptococcus mutans T8 Are Clustered and Can Be Transferred En Bloc

Applied and Environmental Microbiology (1999)

Ping Chen Fengxia Qi Jan Novák Page W. Caufield

ABSTRACT Mutacin II is a ribosomally synthesized peptide lantibiotic produced by group II Streptococcus mutans . DNA sequencing has revealed that the mutacin II biosynthetic gene cluster consists of seven specific open reading frames: a regulator ( mutR ), the prepromutacin structural gene ( mutA ), a modifying protein ( mutM ), an ABC transporter ( mutT ), and an immunity cluster ( mutFEG ). Transformations of a non-mutacin-producing strain, S. mutans UA159, and a mutacin I-producing strain, S. mutans UA140, with chromosomal DNA from S. mutans T8 with an aphIII marker inserted upstream of the mutacin II structural gene yielded transformants producing mutacin II and mutacins I and II, respectively.

Lantibiotics

Gene cluster

Streptococcus sobrinus

Structural gene

10.1128/aem.65.3.1356-1360.1999

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On-line Analysis of Flavor Compounds in Toothpastes by Single Photon Ionization Mass Spectrometry

Xie Yuan-Yuan Lei Hua Keyong Hou Ping Chen Huapeng Cui

A new method was developed for the on-line analysis of flavor compounds in toothpastes without any sample preparation by direct injection time-of-flight mass spectrometry with single photon ionization (SPI-TOFMS). The samples were directly taken into the ionization source by the quartz capillary tube and the pressure condition of the ionization source was optimized. Under the discrimination of characteristic mass spectra of flavor compounds, rapid and accurate identification of flavors in toothpastes was achieved. The analysis time for a single sample was only 0. 5 min. This method was applied to on-line and in-situ monitoring of flavor compounds during tooth brushing process. The influence of water content on release of flavor compounds from toothpastes was studied. The results indicate that direct injection SPI-TOFMS can monitor the composition and concentration of flavor compounds in real time. The method could be suitable to analyze the flavors from various samples such as food and cosmetics.

Sample Preparation

Ambient ionization

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Practice of situational teaching in curriculum of community health nursing

Zhonghua yixue jiaoyu tansuo zazhi (2011)

Xiu-na Liu Ping Chen Xianyuan Wang Juan Zhou

The curriculum of Communitity Health Nursing has fine operation and practicability.Aiming at this point, the situational teaching method is used in teaching.This method is uniquely excellent in arousing students enthusiasm of learning,improving students' ability to integrate theory with practice, cultivating students' creative thinking and team spirit. The teaching effect has been proved correct in our practice. Key words: Communitity health nursing; Nursing education; Situational teaching

Situational ethics

10.3760/cma.j.issn.2095-1485.2011.06.040

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Survey and analysis of disaster cognition of community nurses in Chongqing

Zhonghua xiandai huli zazhi (2012)

Ping Chen Yu Luo

Objective To realize the community nurses' cognitive condition of disasters nursing,so as to provide the basis for disaster nursing training.Methods Convenience sampling was used to investigate the disaster cognition of 120 nurses in 15 community health service centers (stations) in Chongqing.Results Totalof hundred and twenty questioniare were distributed and one hundred and sixteen questionnaires were retured and the effective rate of retrieve was 97％.97.4％ of community nurses showed concerns for disasters and took a positive attitude on dealing with disasters in the future.Their acquaintances with disasters nursing knowledge and nursing technology was 79.3％ and 76.7％ respectively,but they have a relatively lower cognition in disaster rescue principle with 14.7％ and disaster psychological crisis intervention with 9.5 ％.Conclusions To strengthen the education and publicity of disaster nursing,and to enhance the training of disaster rescue knowledge and the research of method is important. Key words: Community nurse; Disaster nursing; Cognition

Community nursing

10.3760/cma.j.issn.1674-2907.2012.35.007

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Psychological detachment from work and prison police's life satisfaction: Role of work family conflicts.

Zhongguo linchuang xinlixue zazhi (2017)

Su-rong Li Ping Chen Peng Liu Minghui Wang

Work–family conflict

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Imaging manifestation of pancreatoblastoma in children

Zhōnghuá xiāohuà wàikē zázhì/Zhonghua xiaohua waike zazhi (2011)

Gang Wu Lan Xiang Jun Xie Bin Xie Yongliang Tang

Pancreatoblastoma is a rare kind of malignant tumor of pancreas,which is commonly seen among children.A female child aged 5 years was admitted to the Dapiag Hospital with the chief complaint of painless abdominal mass on June 18,2010.The results of B ultrasound showed acoustic shadow of tumor calcification and an absent of normal pancreatic echo.The results of computed tomography(CT)showed that the tumor was located at the body and tail of the pancreas with a low-density and isopycnic shadow of intermixed huge block.The parenchyma of tumor showed unequal enhancement,the periphery showed lobular-like reticular enhancement,the central necrotic area showed no enhancement,and multiple metastatic foci were observed in spleen and liver under enhanced CT scan.The patient underwent resection of the body and tail of the pancreas and the spleen.Chemotherapeutics with vincristine,cyclophosphamide and doxorubicin were adopted postoperatively.After a period of 6-month follow-up,the results of CT showed that the size of tumor was decreased. Key words: Pancreatoblastoma; Computed tomography; Children

Reticular connective tissue

Acoustic shadow

Abdominal mass

Parenchyma

10.3760/cma.j.issn.1673-9752.2011.04.026

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An experimental study on protective effect of carbon monoxide releasing molecule on severe acute pancreatitis induced lung injury

Zhonghua gan-dan waike zazhi (2010)

Ping Chen Gang Wang Hua Chen Bei Sun Hongchi Jiang

Objective To investigate the protective effect of carbon monoxide releasing molecule (CORM-2) on severe acute pancreatitis (SAP) induced lung injury in rats.Methods Thirty male Wistar rats were randomly divided into three groups (n= 10) including sham group, SAP group and CORM-2 group.The model of SAP was induced by retrograde infusion of 3.5% sodium taurocholate into the pancreatobiliary duct.CORM-2 (8 mg/kg) was infused through the dorsal artery of penis 0.5 h after establishing the model of SAP in CORM-2 group.All animals were sacrificed 6 h after SAP in-duction.The serum level of tumor necrosis factor-α (TNF-α), pulmonary cytokine induced neutrophil chemoattractant (CINC), intercellular adhesion molecule-1 (ICAM-1) mRNA expression as well as the activity of myeloperoxidase (MPO) in the lung were examined in each group.The wet/dry ratio of the lung was also determined.The lung was scored on the basis of pathological changes.Results Compared with SAP group, pulmonary over-expression of CINC and ICAM-1 mRNA was obviously inhibited in CORM-2 group and the serum TNF-α level, the MPO activity in lung tissue, the wet/dry ratio of lung, and the pathological score of lung injury were significantly lower (P＜0.05).Conclusion Administration of CORM-2 can remarkably reduce the severity of SAP induced lung injury through in-hibiting the over-expression of CINC and ICAM-1 mRNA, down-regulating the serum TNF-α level and subsequently decreasing pulmonary neutrophil infiltration. Key words: Pancreatitis; Carbon monoxide releasing molecule; Lung injury; Cytokine in-duced neutrophil chemoattractant; Intercellular adhesion molecule-1

10.3760/cma.j.issn.1007-8118.2010.03.012

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Minimally invasive percutaneous nephrolithotomy holmium laser lithotripsy guided by ultrasound in the lateral position: report of 64 cases

Harbin Yike Daxue xuebao (2011)

Ping Chen

Objective To investigate the effectivity and safety of minimally invasive percutaneous nephrolithotomy with holmium laser lithotripsy in the lateral position.Methods Sixty-four patients(36 males and 28 females) with kidney stones were performed ureter tube in diseased side,then changing to the lateral position with ultrasound-guided renal biopsy performed.Finally,minimally invasive percutaneous nephrolithotomy access was establishment and stone was observed with wolf 8.5 hard nephrolithotomy.The stone was crushed by holmium laser and pumped out by infusion.Results Among the 64 patients,61 were performed with first operation and 3 cases were underwent second stage surgery because of renal empyema lien.The mean operation time was 50~220 min(109 min±47.5 min).Blood loss was about 60~700 mL(196.4 mL±49.1 mL).The one-stage cure rate was 85.2%(52/61) and the second-stage was 95.3%(61/64).The renal pelvis obstruction discharge rate was 100%,renal insufficiency were recovered without no pleural and peripheral organ damage.Conclusion The lateral decubitus can reduce the anesthetic requirements,improve the operation safety and is suitable for patients with respiratory and circulatory system.It is worth to promote this skill due to suitable for ultrasound-guided puncture and avoiding the X-ray radiation.

Prone position

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