In the present study, the Acetyl-coenzyme A carboxylase (ACCase) of Chlorella sp. USTB-01 purified and characterized. Molecular weight of ACCase was calculated to be about 456 kD and was comprised of two identical subunits of 229 kD. The enzymatic characteristics isoelectric point was 6.7. Moreover, a rapid and sensitive assay of High performance liquid chromatography (HPLC) was established to measure overall activity of ACCase in low protein content condition by detect the ACCase activity by measuring ADP production and Acetyl-coenzyme A (Acetyl-CoA).
A new type of japonica photo(thermo)-sensitive cytoplasmic male sterile line 2310SA was bred.This line contains photo(thermo)-sensitive genic male sterile(PTGMS)genes in the two-line system hybrid rice and cytoplasmic male sterile(CMS)genes in the three-line system hybrid rice,which has a great application potential in hybrid rice breeding for its stable sterility.To understand the underlying genetic mechanism and to explore male sterility of rice,the selected 2310SA line and its maintainer line of the two-line system,PTGMS line 2310S,were grown in Hefei,China.The anthers were collected at four panicle developmental stages(meiosis,mononucleate,binucleate and mature stages)and the isolated RNA were used for microarray analysis.Results showed that differentially expressed genes(DEGs)at meiosis stage(1938genes up-regulated,1635genes down-regulated)and mature stage(2220genes up-regulated,2656genes down-regulated)were more than those at mononucleate stage(752genes up-regulated,693genes down-regulated)and binucleate stage(1025genes up-regulated,886 genes down-regulated).The number of down-regulated genes was higher than that of up-regulated genes at mature stage,whereas at other stages the number of up-regulated genes was more than that of down-regulated genes.Moreover,hierarchical cluster analysis of all DEGs suggested that the expression of the DEGs at mononucleate and binucleate stages belonged to the same cluster,and the expression of the DEGs at meiosis and mature stages to another two clusters.Among DEGs,147genes were found to be differentially expressed at all the stages when comparing 2310SA line with the control line 2310S.For the 147 common DEGs,the down-regulated genes were more than the up-regulated ones at mature stage but not at other three stages.Additionally,these common DEGs were involved in many biological processes,which were classified into 11functional categories,including biosynthesis,response to stimulus,photosynthesis,macromolecule metabolic process,signal transduction,reg-ulation of transcription,carbohydrate metabolic process,flower development and cell death.Among proteins coded by the common DEGs,cytochrome P450family protein and betaring hydroxylase play important roles in fatty acid metabolism,and heat shock transcription factor 29,heat shock protein DnaJ family protein and heat shock protein 70are involved in stress response.These genes are likely to relate directly or indirectly to cell death and pollen abortion.
Objective To investigate the roles of reactive oxygen species (ROS) in acute retention bladder in rats. Methods A total of 25 female Wistar rats were randomized into 5 groups: group 1 without overdistention (OD) (group C, n=5), group 2 with 2 h OD (group E0, n=5), group 3 with 2 h OD and 1 h recovery after emptying (group E1, n=5), group 4 with 2 h OD and 2 h recovery (group E2, n=5). Rats in group 5 with 2 h OD and 2 h recovery (group SOD, n=5) were injected with 500 U superoxide dismutase (SOD) through the cardiac ventricle at 10 min before emptying. Cystometrography was performed in pre-overdistention and post-emptying. Then bladders were excised and frozen in liquid nitrogen for determination of the activity of adenosine triphosphatase (ATPase) and concentration of malondialdehyde (MDA). Results The compliance of post-overdistention bladder decreased sharply as compared with that of the pre-overdistention and overdistention bladder, while frequency of over-activity was higher than that of the pre-overdistention and overdistention bladder. Furthermore, the activity of 3 kinds of ATPase in the bladder in group E1 reduced sharply. The concentration of MDA increased during overdistention period and early period of emptying and peaked at 1 h after emptying. After injection of superoxide dismutase, the concentration of MDA in group SOD bladder was lower than that in group E2 bladder. At the same time, the bladder function was improved. Conclusion ROS is a main factor contributing to injury of overdistention/ emptying bladder. Antioxidant can protect bladder function from injury of over-distention.
Objective To explore the changes of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),nitric oxide(NO)and nitric oxide synthase(NOS)in maternal and umbilical cord blood with asphyxia neonatorum.Methods 60 cases subjected to acute fetal distress were divided into fetal distress group in which the neonatus is normal and asphyxia neonatorum group in which the neonatus has asphyxiation with 30 in each group.Thirty cases that pregnant woman and neonatus were both healthy serve as control.After childbirth,Apgar score of 1 minute is assessed and pH values of umbilical cord blood are measured quickly for the determination of neonatal asphyxia.And then the contents of TNF-α,IL-6 and NO and the activity of NOS are detected in the maternal and umbilical cord blood.Results There are no statistically significant differences in the levels of TNF-α,IL-6,NO and NOS of maternal blood among three groups.The pH in asphyxia neonatorum group was lower than that in other two groups.The levels of TNF-α,IL-6 and NO in umbilical cord blood of asphyxia neonatorum group were increased obviously compared with those of fetal distress group and control group,the activity of NOS in asphyxia neonatorum group was increased obviously than that of control group,and those indexes of fetal distress group are markedly higher than those in control group.Conclusion The inflammation mediators such as TNF-α,IL-6 and NO were increased in umbilical cord blood of asphyxia neonatorum,and that provides experiment basis for the treatment of asphyxia neonatorum.
Objective To explore the clinical application of the serum allergen-specific IgE and IgG tests in children with atopic dermatitis.Methods The specific IgG antibodies for food-allergens detected by the ELISA method and the specific IgE antibodies for either food-or aero-allergens determined by the western blot way were undertaken in 64 children with atopic dermatitis.Results There was the significant difference between the testing results of the specific IgG and IgE for food-allergens(P0.01).The positive rate of the specific IgG was 93.75% for food-allergens which were mainly milk and egg,while the positive rate of the specific IgE was 46.88% for food-allergens which were mainly egg,fish,shrimp and crab,respectively.The positive rate of the specific IgE was 34.38% for aero-allergens which were mainly dust mites and molds.It was common seen that the positive results of the specific IgE for food-allergen in infants under one-year old and of the specific IgE for aero-allergens in infants of one year and upwards,the latter usually manifested asthma and/or rhinitis(P0.05).Conclusions It is suggested that the either food-allergens or aero-allergens is important cause to result in atopic dermatitis in children.It will be feasible to detect both of the specific IgE and IgG for the identification of food-allergens in children with allergic skin diseases.The early detection of aeroallergens and the effective improvement of the environment shall be necessary for the management and preventions of allergic skin and resperatory diseases in children.
Objective: To discuss the role of pleural biopsy and detecting pleural level of CA 50 \, TSA\, SF\, CEA\, chromosome analysis in differentiation of benign from malignant hydrothorax. Methods: 40 patients of tuberculous hydrothorax (group A) and 34 patients of malignant hydrothorax (group B) were taken pleural biopsy and detected the pleural level of CA 50 ?TSA?CEA?SF with radioimmunoassay, routine chromosome preparation, and compared the difference of the level of above\|mentioned markers, estimated the positive rate and specific of above\|mentioned markers. Results: The positive rate of pleural biopsy in patients with tuberculous and malignant hydrothorax were 60% and 50%. The values of CA 50 ?TSA?CEA?SF and chromosome analysis in group B were significantly higher than those in group A (P0 001 and P0 01). The sensitivity of using a combination of the CA 50 ?TSA?CEA?SF?chromosome analysis assays and a combination of the CA 50 ?CEA?chromosome analysis assays were increased to 88% and 76%, there was significant difference (P0 01). The specific of combination assay of 3 markers was 95%, it was significantly higher than that of single assay (P0 05). The specific of combination assay of 3 markers was significantly heiger than that of 5 markers (P0 01). Conclusion: The results suggest that determination of CA 50 ?TSA?CEA?SF and chromosome analysis are better for the differentiating benign from malignant hydrothorax. The missed diagnosis rate of combination assay of 5 markers may produce a significant decrease in patients with malignant hydrothorax. CEA\, CA 50 in combination with chromosome analysis assay may be a better indicator for screening the malignant hydrothorax.\;
and their subfractions.Methods: The herbs were extracted with 95%ethanol and water successively.The ethanol-extract(A) was further extracted with petholeum ether,ethyl acetate,n-butanol and water successively to yield four subfractions(A-a,A-b,A-c,A-d).The water extract (B) was precipitated with 3-time volume of ethanol to be divided into two parts(B-a,B-b).The chemical compositions of these subfractions were analyzed by TLC and the antibacterial activities were investigated in vitro by 2-time dilution method.Result: The A-d of the ethanolextract showed strong activities against Staphylococcus aureus,Escherichia coli and Pseudomonas aeruginosa.Conclusion: The herbs of Prunella vulgaris L.possess antibacterial activity.
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