Aiqin Liu

Harbin Medical University

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Yujuan Shen

National Institute for Parasitic Diseases

Jianping Cao

National Institute for Parasitic Diseases

Fengkun Yang

Harbin Medical University

Longxian Zhang

Henan Agricultural University

Rongjun Wang

Henan Agricultural University

Weizhe Zhang

Shanghai Advanced Research Institute

Weizhe Zhang

Harbin Institute of Technology

Hong Ling

Fuzhou University

Wei Zhao

State Key Laboratory of Medicinal Chemical Biology

Baiyan Gong

Southern Medical University

Cooperative Institutions

Chinese Academy of Sciences

Harbin Medical University

Fujian Agriculture and Forestry University

Sun Yat-sen University

Henan Agricultural University

University of Chinese Academy of Sciences

Ministry of Agriculture and Rural Affairs

Shanghai Jiao Tong University

Capital Medical University

National Institute for Parasitic Diseases

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Changes in soil physico-chemical properties following vegetation restoration mediate bacterial community composition and diversity in Changting, China

Ecological Engineering (2019)

Xiaolong Hou Hang Han Mulualem Tigabu Liping Cai Fan‐Rui Meng

Acidobacteria

Soil carbon

10.1016/j.ecoleng.2019.07.031

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Citations (50)

酸性土壌におけるグリホサートとグリホサートの吸着脱着相互作用機構を研究した。【JST・京大機械翻訳】

Nongye Huanjing Kexue Xuebao (2016)

Zhou Chuifan Lin Jingwen Li Ying Aiqin Liu

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Study on selection of high-nitrogen-efficiency-genotypes of Chinese fir clones

Linye Kexue (2002)

Xiangqing Ma Aiqin Liu Baolong Huang Youli Chen

The results were as follows: there were significant differences in nitrogen utilization efficiencies among different Chinese fir clones. As the degree of nitrogen stress increased, nitrate reductase activities and light saturation points of different clones decreased, CO_(2) compensation pints increased and responses of different clones to nitrogen stresses became more obvious. The tested cloens may be classified into three categories: strong, weak and intermedite capacity to nitrogen stresses according to their responses to nitrogen stress. Among the tested clones, No. 163 and No. 301 were the High-nitrogen-efficiency clones that could produce high yield in the site of low nitrogen concentration and ought to be popularized for poor site. These clones have practical significance to buffer the increasingly sever soil degradation and provide a new approach to control the productivity decline for successive planting of Chinese fir.

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Cryptosporidium cuniculus and Giardia duodenalis in Rabbits: Genetic Diversity and Possible Zoonotic Transmission

PLoS ONE (2012)

Weizhe Zhang Yujuan Shen Rongjun Wang Aiqin Liu Hong Ling

Background Cryptosporidium and Giardia are the two important zoonotic pathogens causing diarrhea of humans and animals worldwide. Considering the human cryptosporidiosis outbreak and sporadic cases caused by C. cuniculus, the important public health significance of G. duodenalis and little obtained information regarding rabbit infected with Cryptosporidium and Giardia in China, the aim of this study is to determine the prevalence and molecularly characterize Cryptosporidium and Giardia in rabbits in Heilongjiang Province, China. Methodology/Principal Findings 378 fecal samples were obtained from rabbits in Heilongjiang Province. Cryptosporidium oocysts and Giardia cysts were detected using Sheather's sugar flotation technique and Lugol's iodine stain method, respectively. The infection rates of Cryptosporidium and Giardia were 2.38% (9/378) and 7.41% (28/378), respectively. Genotyping of Cryptosporidium spp. was done by DNA sequencing of the small subunit rRNA (SSU rRNA) gene and all the nine isolates were identified as Cryptosporidium cuniculus. The nine isolates were further subtyped using the 60-kDa glycoprotein (gp60) gene and two subtypes were detected, including VbA32 (n = 3) and a new subtype VbA21 (n = 6). G. duodenalis genotypes and subtypes were identified by sequence analysis of the triosephosphate isomerase (TPI) gene. The assemblage B (belonging to eight different subtypes B-I to B-VIII) was found in 28 G. duodenalis-positive samples. Conclusions/Significance The rabbits have been infected with Cryptosporidium and Giardia in Heilongjiang Province. The results show that the rabbits pose a threat to human health in the studied areas. Genotypes and subgenotypes of C. cuniculus and G. duodenalis in this study might present the endemic genetic characterization of population structure of the two parasites.

Giardia

Giardia lamblia

10.1371/journal.pone.0031262

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KR-DBSCAN: A density-based clustering algorithm based on reverse nearest neighbor and influence space

Expert Systems with Applications (2021)

Lihua Hu Hongkai Liu Jifu Zhang Aiqin Liu

DBSCAN

Nearest-neighbor chain algorithm

10.1016/j.eswa.2021.115763

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First identification and genotyping of Enterocytozoon bieneusi in humans in Myanmar

BMC Microbiology (2020)

Yujuan Shen Baiyan Gong Xiaohua Liu Yanchen Wu Fengkun Yang

Abstract Background Intestinal pathogen infections are widespread among impoverished populations. Enterocytozoon bieneusi is the most common pathogen of intestinal microsporidian species in humans worldwide . However, no epidemiological information is available on E. bieneusi infection in humans in Myanmar. The present study comprised the first identification and genotyping of E. bieneusi in humans conducted in Myanmar. Results A total of 172 fecal specimens were collected from the Wa people (one each) in four villages of Pangsang Township of the Matman District of Shan State, Myanmar, and each participant completed a questionnaire. E. bieneusi was identified and genotyped using polymerase chain reaction (PCR) and sequence analysis of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. The average prevalence of E. bieneusi was 8.72% (15/172), ranging from 3.85 to 13.89% by village. E. bieneusi infection was not related to any of the risk factors studied. Six genotypes were identified, comprising two known genotypes Peru6 ( n = 10) and D ( n = 1) and four novel genotypes (MMR23, MMR25, MMR86, and MMR87) (one each), and two people infected with genotype Peru6 were from the same family. A phylogenetic analysis based on a neighbor-joining tree of the ITS sequences of E. bieneusi indicated that all the six genotypes were clustered into group 1. Conclusions This is the first identification and genotyping of E. bieneusi in humans in Myanmar. The observations that the two people infected with genotype Peru6 were from the same family, and that all six genotypes obtained in the present study fell into zoonotic group 1, showed the potential for anthropogenic and zoonotic transmissions. The present data argue for the importance of epidemiological control and prevention from medical sectors.

Enterocytozoon bieneusi

Molecular Epidemiology

10.1186/s12866-019-1694-1

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Nutrient cycling and distribution in different-aged plantations of Chinese fir in southern China

Forest Ecology and Management (2007)

Xiangqing Ma Kate V. Heal Aiqin Liu P. G. Jarvis

Nutrient cycle

Cunninghamia

Cycling

Plant litter

Understory

Litter

10.1016/j.foreco.2007.02.018

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COMPARISON OF THE PHOSPHORUS CHARACTERISTICS OF DIFFERENT CHINESE FIR CLONES

Chinese Journal of Plant Ecology (2006)

Xia Liang Aiqin Liu Xiangqing Ma FENG Li-Zhen Huang Yi-jiang

Background and Aims Phosphorus(P)deficiency is one of the main factors that influence plant productivity in agricultural and forestry systems. Fertilization and soil improvement are the primary practices used to meet the P demands of crops in traditional agriculture and of trees in forestry management. Chinese fir (Cunninghamia lanceolata), a fast_growing, evergreen coniferous tree with high yield and quality of wood, is the most important tree species of timber plantations in subtropical China. Since Chinese fir plantations range from about 20° to 30° N in latitude and 100° to 120° E in longitude, there are many different genotypes of Chinese fir among the forests in south China. Therefore, it is possible to select the Chinese fir clone with high phosphorus use efficiency. Methods Based on the pot experiment under the different phosphorus deficiency stress (heavy, medium, slight phosphorus deficiency and normal phosphorus supply), the dry matter accumulation, phosphorus absorption efficiency, and phosphorus use efficiency of eight different Chinese fir clones were analyzed to compare their phosphorus characteristics. Key Results There were significant differences in the dry matter accumulation, phosphorus absorption efficiency and phosphorus use efficiency among different clones under phosphorus stress. The dry matter accumulation and phosphorus absorption efficiency of different clones decreased whereas phosphorus use efficiency increased with increasing phosphorus stress. The dry matter accumulation of Clone 8, 9, 24 and 37 were influenced by phosphorus stress less than those of the other clones. Phosphorus stress significantly affected the dry matter accumulation of Clone 23. Higher phosphorus absorption and use efficiency in Clone 8, higher phosphorus absorption efficiency in Clone 24 and 37, and higher phosphorus use efficiency Clone 9 were found under phosphorus stress. However, the phosphorus absorption efficiency and phosphorus use efficiency of Clone 23 were lower than those of the other 7 clones. Conclusion High phosphorus absorption and use efficiency of Chinese fir are the major adaptive strategy under environment with low phosphorus availability.

Douglas fir

10.17521/cjpe.2006.0129

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Citations (4)

Itemized determination of As, Sb, Bi, Se, and Ge in graphite sample by HG-AFS with high-temperature microwave digestion

Analytical Sciences (2024)

Jianwei Meng Liping Wang Aiqin Liu Yan-Qiao Hu Wei Li

In this paper, we optimized a method for fast and accurate determination of five impurity elements (As, Sb, Bi, Se, and Ge) in graphite samples to overcome the shortcomings of existing methods, such as complicated equipment, cumbersome process, multiple-time preparation, separate determination, and large error in results. Graphite samples were digested with HNO3–H2SO4–HClO4–HF in a high-temperature and high-pressure microwave digestion apparatus, and the elements were extracted and determined separately by AFS (atomic fluorescence spectrometry). There is no element loss during the processing and analysis of this method. The spike recoveries (As: 90.30%–102.3%, Sb: 90.73%–110.0%, Bi: 90.00%–99.67%, Se: 93.33%–110.0%, Ge: 92.26%–104.2%) and precision (RSD%; As: 1.34%–8.96%, Sb: 2.67%–7.10%, Bi: 1.83%–4.58%, Se: 0.36%–3.25%, Ge: 4.41%–8.65%) meet the requirements of the corresponding quality specifications. The method has some advantages (such as no elemental loss, fast testing, strong element targeting, and accurate results), and thus can achieve batch determination of graphite samples. The optimized method for graphite sample and final solution preparations can be used for diverse spectrometric technologies, and that for spectrometer conditions have reference value for HG-AFS instruments.

Microwave digestion

Sample Preparation

Digestion

10.1007/s44211-024-00526-6

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Molecular identification of Cryptosporidium ryanae isolate from dairy cows in Harbin.

Zhongguo renshougonghuanbing zazhi (2010)

Xiang Yi Yang Feng-kun LI Yi-hong Hong Ji Shu Jing

One Cryptopsoridium isolate from a 3 month-old calf in a dairy cow farm of Harbin was identified by molecular method,in which the common genes of 18S rRNA and actin used in Cryptosporidium genotyping were selected,the primers were designed,and the approximate 840 bp and 1066 bp fragments of these two genes were amplified respectively by nested PCR.The PCR products were sequenced by two directions,and then the sequences were analyzed by using the soft wares of ClustlX,DNAstar,and PHLIP.The experimental result showed that in the 18SrRNA gene,there was the highest identities compared to other reported isolates of cow-derived C.ryanae(DQ871345,AY587166,and EU410344),with 100% of similarity.Likewise,at actin locus,the present isolate also had 100% similarity with an American cow-derived C.ryanae isolate(EU410345) and a deer-like genotype isolate(AY741309).Phylogenetic analysis indicated that the present isolate shared the same evolution branch in the two gene loci.These analyses demonstrated that the present isolate belonged to C.ryanae.These results provide a significant reference for the study on the species distribution and assessment in the public health significance of Cryptosporidium in cow milk in China.

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Citations (1)