Zhaohua Liu

Southern University of Science and Technology

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Zhibin Ji

Shandong Agricultural University

Guizhi Wang

Xuzhou Medical College

Jianmin Wang

Roswell Park Comprehensive Cancer Center

Tianle Chao

Shandong Agricultural University

Lei Hou

Northeast Agricultural University

Liang Xiong

Chengdu University of Traditional Chinese Medicine

Qin‐Mei Zhou

Chengdu University of Traditional Chinese Medicine

Cheng Peng

First Affiliated Hospital of Jinan University

Rong Xuan

Jilin University

Aili Wang

Columbia University Irving Medical Center

Cooperative Institutions

Chinese Academy of Sciences

Shandong Agricultural University

Chengdu University of Traditional Chinese Medicine

Shandong University

Ministry of Agriculture and Rural Affairs

Chinese Academy of Tropical Agricultural Sciences

University of Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

University of Hong Kong

Peking University

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Additional file 5 of Genome‑wide integrated analysis demonstrates widespread functions of lncRNAs in mammary gland development and lactation in dairy goats

Figshare (2020)

Zhibin Ji Tianle Chao Zhaohua Liu Lei Hou Jin Wang

Additional file 5: Table S4. Differentially expressed mRNAs between different lactation periods.

Mammary tissue

10.6084/m9.figshare.12023682

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Transcriptome Analysis of Dairy Goat Mammary Gland Tissues from Different Lactation Stages

DNA and Cell Biology (2019)

Zhibin Ji Tianle Chao Chunlan Zhang Zhaohua Liu Lei Hou

The mammary gland is a unique organ involved in lactation in dairy livestock, and its development and lactation ability are affected by both genetic and environmental factors. To explore the molecular regulatory mechanisms of these factors, this study used high-throughput sequencing technology and bioinformatics methods to systematically analyze the transcriptome of Laoshan dairy goat mammary gland tissues from different lactation stages. From three libraries, 36,336,892, 36,469,596, and 35,759,380, pure sequences were obtained, with 25,292, 23,665 and 27,220 expressed genes, respectively, resulting in a total of 14,892 nonredundant differentially expressed genes. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that these genes were annotated to biological regulation, cellular processes, metabolic processes, cells, organelles, binding, catalytic activity and transcriptional activity, and so on. The genes were mainly involved in cellular processes, environmental information processes, genetic information processes, human diseases, metabolism, and organ systems. Finally, 20 genes related to mammary gland development and lactation were screened to construct a gene regulatory network. These findings support the involvement of the constructed network in the regulation of mammary gland development and lactation, and they not only lay the foundation to further investigate and screen the main genes or molecular genetic markers controlling the development of the mammary gland and lactation ability of dairy goats but also increase the in-depth understanding of the lactation physiology of dairy goats.

KEGG

10.1089/dna.2018.4349

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Citations (15)

[Expression of alpha, beta and gamma subunits of epithelial sodium channel in the cochlea and endolymphatic sac of guinea pig].

PubMed (2009)

Shixun Zhong Zhaohua Liu

To investigate the distribution and role of alpha, beta and gamma subunits of epithelial sodium channel (ENaC) in the cochlea and endolymphatic sac of guinea pig.The expression of alpha-, beta- and gamma-ENaC subunits proteins was studied by immunohistochemistry with the specific polyclonal rabbit antibodies against the alpha, beta and gamma subunits of rat ENaC. Alpha-ENaC mRNA was detected by in situ hybridization with digoxin labeled cDNA probe.All three subunits of ENaC, alpha-, beta- and gamma-, were widely distributed in the labyrinth. In the cochlea, strong labeling of alpha-ENaC protein was found in the spiral limbus, and to a less extent, in the spiral ligament, organ of Corti and Reissner's membrane. The immunoreactivity of beta-ENaC was observed in the spiral ligament, spiral limbus, spiral ganglion, organ of Corti and Reissner's membrane with a less intensity than that of alpha-ENaC. Gamma-ENaC was presented primarily in the superior part of the spiral ligament, spiral limbus, spiral ganglion, and weakly in the organ of Corti and Reissner's membrane. In the endolymphatic sac, intensive immunoreactivities of all three subunits were seen in the epithelial cells and the subepithelial cells at similar intensity. Alpha-ENaC mRNA was localized in the spiral limbus, the inferior part of spiral ligament, stria vascularis, and epithelial cells and subepithelial cells of endolymphatic sac.Different subunits of the ENaC expressed in various cell regions of the cochlea and endolymphatic sac in distinct patterns may form the functional sodium channel to regulate the endolymph, thus serve to maintain homeostasis in inner ear.

Spiral ganglion

Spiral ligament

Epithelial sodium channel

Endolymph

Endolymphatic sac

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Leonuketal, a Spiroketal Diterpenoid from Leonurus japonicus

Organic Letters (2015)

Liang Xiong Qin‐Mei Zhou Yike Zou Minghua Chen Li Guo

An architecturally complex spiroketal diterpenoid, leonuketal (1), was isolated from the aerial parts of the plant Leonurus japonicus. This compound possessed an unprecedented tetracyclic skeleton that comprised a bridged spiroketal moiety fused with a ketal-γ-lactone unit. The structure and absolute configuration were determined by spectroscopic analyses, a modified Mosher's method, and ECD (electronic circular dichroism) calculations. Leonuketal (1) showed significant vasorelaxant activity against KCl-induced contraction of rat aorta, with the EC50 value of 2.32 μM.

Moiety

Absolute Configuration

Terpene

10.1021/acs.orglett.5b03227

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Citations (49)

Treatment and prevention of delayed hemorrhage after transurethral resection of prostate

Guoji yiyao weisheng daobao (2014)

Zirong Huang Zhijian Yang Zhaohua Liu Bing-quan Wu Minjian Chen

Objective To study the treatment and prevention of delayed hemorrhage after the transurethral resection of prostate.Methods 35 patients with delayed hemorrhage after the transurethral resection of prostate were retrospectively analyzed.Results 35 cases were cured after positive disposition and discharged.27 cases received conservative treatment; 7 cases of severe hemorrhage required transfusion therapy; and 6 cases had emergency surgery to clear the blood clots in bladder and received resectoscopic hemostatic treatment.Conclusions Intraoperative incomplete hemostasis,excessive residual gland,incompletely cleared tissue debris,eschar shedding,over activity of bladder,lower urinary tract irrfections,astriction,and other factors lead to delayed hemorrhage.Delayed hemorrhage is preventable.If it is cured timely,the prognosis is good. Key words: TURP; Delayed hemorrhage; Treatment; Prevention

Clearance

Hemostatic Agent

10.3760/cma.j.issn.1007-1245.2014.18.015

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Relation between accumulation of risk factors for ischemic cardiovascular diseases and healthy behaviors in military cadres

Xinxueguan kangfu yixue zazhi (2012)

Zhaohua Liu

Objective: To study relation between accumulation of risk factors for ischemic cardiovascular diseases and healthy behaviors in military cadres.Methods: Epidemiological survey on risk factors for ischemic cardiovascular diseases was performed in 977 cadres in-service(≥35 years old) from a unit.Health promoting lifestyle profile Ⅱ(HPLP Ⅱ) was used to survey healthy behaviors,and the results were analyzed statistically.Results: There were significant differences in six dimensions of healthy behaviors(F=3.467,P=0.004),health responsibility(F=4.516,P=0.000),sports exercise(F=2.949,P=0.012),nutrition(F=6.299,P=0.000),self-realization(F=3.778,P=0.002) and interpersonal relationship(F=4.546,P=0.000) in HPLPⅡ among 0-5 risk factors accumulation groups.Score of healthy behaviors in three risk factors accumulation group was significantly lower than those of other accumulation groups.Conclusion: Aiming at strengthening health education and establishing good healthy behaviors can prevent and treat ischemic cardiovascular diseases in accumulated population with three risk factors.

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Metabolic pathways leading to detoxification of triptolide, a major active component of the herbal medicine Tripterygium wilfordii

Journal of Applied Toxicology (2013)

Fuying Du Zhaohua Liu Xinxiu Li Jie Xing

ABSTRACT Triptolide (TP) shows promising anti‐inflammatory and antitumor activity but with severe toxicity. TP is a natural reactive electrophile containing three epoxide groups, which are usually linked to hepatotoxicity via their ability to covalently bind to cellular macromolecules. In this study, metabolic pathways leading to detoxification of TP were evaluated in glutathione (GSH)‐depleted (treated with L‐buthionine‐S,R‐sulfoxinine, BSO) and aminobenzotriazole (ABT; a non‐specific inhibitor for P450s)‐treated mice. The toxicity of TP in mice was evaluated in terms of mortality and levels of serum alanine transaminase (ALT). In incubates with NADPH‐ and GSH‐supplemented liver microsomes, seven GSH conjugates derived from TP were detected. In mice, these hydrolytically unstable GSH conjugates underwent γ‐glutamyltranspeptidase/dipeptidases‐mediated hydrolysis leading to two major cysteinylglycine conjugates, which underwent further hydrolysis by dipeptidases to form two cysteine conjugates of TP. In ABT‐treated mice, the hydroxylated metabolites of TP were found at a lower level than normal mice, and their subsequent conjugated metabolites were not found. The level of cysteinylglycine and cysteine conjugates derived from NADPH‐independent metabolism increased in mice treated with both TP and BSO (or ABT), which could be the stress response to toxicity of TP. Compared with normal mice, mortality and ALT levels were significantly higher in TP‐treated mice, indicating the toxicity of TP. Pretreatment of ABT increased the toxicity caused by TP, whereas the mortality decreased in GSH‐depleted mice. Metabolism by cytochrome P450 enzymes to less reactive metabolites implied a high potential for detoxification of TP. The GSH conjugation pathway also contributed to TP's detoxification in mice. Copyright © 2013 John Wiley & Sons, Ltd.

Triptolide

Buthionine sulfoximine

Tripterygium wilfordii

Detoxification

Acetaminophen

10.1002/jat.2906

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Citations (41)

Expression patterns of Nedd4 isoforms and SGK1 in the rat cochlea

Acta Oto-Laryngologica (2008)

Shixun Zhong Zhaohua Liu

Our findings suggest that there exists a Na(+) transport system in the cochlea consisting of SGK1, Nedd4 isoforms and ENaC, which may work in concert to transport Na(+) and to maintain homeostasis in the inner ear as they do in other tight epithelia.It has been well demonstrated that the epithelial sodium channel (ENaC) plays important roles in regulation of Na(+) reabsorption in the tight epithelia of kidney, colon and lung. Recent studies have provided multiple evidence that the serum glucocorticoid-inducible kinase1(SGK1) and the ubiquitin-protein ligase neural precursor cell-expressed, developmentally downregulated isoforms Nedd4 are involved in ENaC regulation in response to hormones such as aldosterone, vasopressin and insulin. The authors aimed to investigate the cellular localization of Nedd4 isoforms, Nedd4-1/2 and Nedd4-2, and SGK1 in the rat cochlea.The expression patterns of Nedd4-1/2, Nedd4-2 and SGK1 in the rat cochlea were studied by immunohistochemistry with the specific polyclonal rabbit antibodies against the rat Nedd4-1/2, Nedd4-2 and SGK1.All three proteins were extensively expressed in various regions of the rat cochlea. They were found in the stria vascularis, spiral ligament, organ of Corti, spiral limbus, spiral ganglion and Reissner's membrane.

SGK1

NEDD4

Expression (computer science)

10.1080/00016480802552501

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Citations (12)

New amides from the roots of Anisodus tanguticus

Biochemical Systematics and Ecology (2020)

Haoyu Zhao Juan Liu Huan Zhu Fei Liu Zhaohua Liu

10.1016/j.bse.2020.104082

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dui jia ben huang suan cui hua zhi bei sheng wu chai you de yan jiu

Gai Yujuan Fengtao Zhan LV Zhifeng Zhaohua Liu Ying Wang

本文研究了花生油与甲醇在催化剂对甲苯磺酸存在下通过单次酯交换反应制备生物柴油的最佳反应条件和两次酯交换对反应的影响。研究结果表明,催化剂用量为油脂质量的0.5%、醇油摩尔比为10∶1、反应温度为150℃、反应1.5h,单次酯交换制备生物柴油,收率可达到93.6%;总催化剂用量为0.5%(占油脂质量)、醇油摩尔比为14∶1、反应温度为150℃、反应4.0h,两次酯交换法制备生物柴油,收率可达到97.9%。

Chai

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