Protease serine 8 (PRSS8), a trypsin-like serine peptidase, has been shown to function as a tumour suppressor in various malignancies. The present study aimed to investigate the expression pattern, prognostic value and the biological role of PRSS8 in human hepatocellular carcinoma (HCC).PRSS8 expression in 106 HCC surgical specimens was examined by Real-time polymerase chain reaction (PCR) and immunohistochemistry, and its clinical significance was analysed. The role of PRSS8 in cell proliferation, apoptosis and invasion were examined in vitro and in vivo.PRSS8 mRNA and protein expression were decreased in most HCC tumours from that in matched adjacent non-tumour tissues. Low intratumoral PRSS8 expression was significantly correlated with poor overall survival (OS) in patients with HCC (P = 0.001). PRSS8 expression was an independent prognostic factor for OS (hazard ratio [HR] = 1.704, P = 0.009). Furthermore, restoring PRSS8 expression in high metastatic HCCLM3 cells significantly inhibited cell proliferation and invasion. In contrast, silencing PRSS8 expression in non-metastatic HepG2 cells significantly enhanced cell growth and invasion. Moreover, our in vivo data revealed that attenuated PRSS8 expression in HepG2 cells greatly promoted tumour growth, while overexpression of PRSS8 remarkably inhibited tumour growth in an HCCLM3 xenograft model. Enhanced cell growth and invasion ability mediated by the loss of PRSS8 expression was associated with downregulation of PTEN, Bax and E-cadherin and an upregulation in Bcl-2, MMP9 and N-cadherin.Our data demonstrate that PRSS8 may serve as a tumour suppressor in HCC progression, and represent a valuable prognostic marker and potential therapeutic target for HCC.
BACKGROUND:Hyperbilirubinemia is associated with central nervous system damage in preterm neonates due to the neurotoxicity of bilirubin. This study explored the possible mechanisms of bilirubin's neurotoxicity, and the protective effect of baicalin (BAI) was also investigated. MATERIAL AND METHODS:Isolated neonatal rat hippocampal neurons were exposed to free bilirubin (Bf). BAI was used to treat these neurons. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the cell viability. Terminal deoxynucleotidyl transferase-dUTP nick-end labeling (TUNEL) assay was used to detect apoptosis. Contents of inflammatory cytokines were determined by enzyme-linked immunosorbent assay (ELISA). Protein expression and phosphorylation levels were assessed by Western blotting. Nuclear translocation was observed by immunofluorescent staining. RESULTS:Bf incubation significantly induced apoptosis and decreased viabilities of neurons. The phosphorylation levels of MAP kinase kinase (MKK)3, MKK6, p38 mitogen- activated protein kinases (MAPK), nuclear translocation level of p65, and the expression levels of cleaved caspase3 and tumor necrosis factor (TNF)α were found to be dramatically higher in Bf-incubated neurons. BAI pre-treatment, however, increased cell viability by reducing cell apoptosis. BAI pre-treatment also reduced phosphorylation levels of MKK3, MKK6, p38 MAPK, and nuclear translocation level of p65, as well as the expression levels of cleaved caspase3 and TNFα, in Bf- incubated neurons. CONCLUSIONS:BAI suppressed bilirubin-induced neuron apoptosis and inflammation by deactivating p38 MAPK signaling.
Objective
To evaluate the impact of the professional knowledge and skills of hospital attendants on the quality of hospital care and its training effect.
Methods
200 accompanists from a Class Ⅲ Grade A hospital in Xi'an from January 2017 to December 2018 were selected as the subjects by convenient sampling. 100 accompanists from January to December 2018 were used as observation groups to develop their professional knowledge and skills training; 100 accompanists who did not carry out professional knowledge and skills training during from January to December 2017 were the control group. The professional knowledge and skills, quality of care were compared between the two groups before and after training.
Results
The scores of basic care, daily life care, psychological care, functional rehabilitation, cleansing care, and communication in the observation group were higher than those in the control group, and the differences between the two groups were statistically significant (P<0.05) . After training, the patients' health education awareness rate, complication rate, safety incident rate, and satisfaction were better than before training, and the differences were statistically significant (P<0.05) .
Conclusions
Due to the limitation of professional knowledge and skill level, the comprehensive quality of hospital accompanists needs to be improved. Strengthening the training of accompanists can not only improve the comprehensive quality of the accompanists themselves, but also improve the quality of hospital care, which is beneficial to the health and rapidity of the medical care industry.
Key words:
Accompanists; Professional knowledge and skills; Quality of hospital care; Training effect
Objective
To explore the effects of scenario simulation combined with case share on fall prevention education in elderly patients.
Methods
A total of 80 elderly patients admitted in Shaanxi Provincial People's Hospital from January 2016 to December 2017 were selected by convenient sampling and equally divided into two groups according to the random number table. Patients in the control group received conventional fall prevention education, while patients in the observation group received fall prevention education which combined scenario simulation and case share. Falls of patients, effects of education and satisfaction were compared between the two groups.
Results
The incidence rate of falls in the observation group during hospitalization was 10.0%, while that in the control group was 20.0% (χ2=4.325; P<0.05) ; the knowledge of fall prevention, the awareness of fall prevention and fall prevention compliance scored (86.3±5.2) , (84.2±5.3) and (91.3±8.4) , higher than those in the control group (t=9.981, 9.040, 12.872; P<0.01) , which were (76.3±4.4) , (75.2±3.4) and (71.3±5.1) respectively. The satisfaction with nursing in the observation group was 95.0%, higher than that in the control group (χ2=4.201; P<0.05) .
Conclusions
Fall prevention education which combines scenario simulation and case share can effectively enhance the awareness of fall prevention, reduce the incidence of falls and improve the satisfaction with nursing in the patients, which is worth promoting in clinical practice.
Key words:
Aged; Accidental falls; Scenario simulation; Fall prevention education; Satisfaction
This paper discussed the question of mental health education on geography instruction. It concluded that: it could practice education through the following ways: taking advantage of the mental resources in geography text to form students' positive emotion, recreating class instruction methods to enrich students' successful feeling, transforming homework style to develope students' study interests, taking the methods of social practice to train students' interpersonal skill.We should pay attention to two points in practice:making good use of each students' individual advantage to increase their self-confidence, building harmonious teacher- student relationship to make students trust their teachers.
To observe and analyze the short-term efficacy of methylprednisolone pulse therapy in the treatment of acute spinal cord injury, and provide valuable guidance for clinical treatment. The patients were divided into study group (100 cases) for methylprednisolone pulse therapy and reference group (100 cases) for dexamethasone therapy. The treatment outcomes of the two groups were statistically compared. Results: Comparison of the overall treatment effectiveness of the two groups shows significant advantage of the study group over the reference group, p <0.05; Conclusion: Methylprednisolone pulse therapy has a good short-term effect on acute spinal cord injury, which can promote patient recovery with great application value.
The aim of the present study was to explore the feasibility of the construction of engineered myocardial tissues in vitro with cardiomyocyte‑like cells derived from bone marrow mesenchymal stem cells (BMMSCs) and a polylactic‑co‑glycolic acid (PLGA) polymer. The PLGA polymer was sheared into square pieces (10x10x1 mm), sterilized by Co60 irradiation, and hydrated in Dulbecco's modified Eagle's medium for 1 h. BMMSCs were isolated from the bone marrow of Sprague‑Dawley rats and the third passage cells were induced by 5‑azacytidine (5‑aza). Following successful induction, the cells were trypsinized and suspended at a density of 1x109/ml. Then, the cell suspension was added to the PLGA scaffold and cultured for 14 days. The morphological changes of BMMSCs were observed using phase contrast microscopy. Immunofluorescence staining was used to identify the cardiomyocyte‑like cells. Hematoxylin and eosin (H&E) and immunohistochemical staining were used to observe the morphology of the engineered myocardial tissues. The cell adhesion rates and scanning electron microscopy were used to observe the compatibility of the cardiomyocyte‑like cells and PLGA. Transmission electron microscopy was used to view the ultrastructure of the engineered myocardial tissues. BMMSCs in primary culture presented round or short spindle cell morphologies. Following induction by 5‑aza, the cells exhibited a long spindle shape and a parallel arrangement. Analysis of the cell adhesion rates demonstrated that the majority of the cardiomyocyte‑like cells had adhered to the PLGA scaffolds at 24 h. H&E staining suggested that the cardiomyocyte‑like cells with spindle nuclei were evenly distributed in the PLGA scaffold. Immunofluorescence staining revealed that the cardiomyocyte‑like cells were positive for cardiac troponin I. Scanning electron microscopy demonstrated that the inoculated cells were well attached to the PLGA scaffold. Transmission electron microscopy indicated that the engineered myocardial tissues contained well‑arranged myofilaments, desmosomes, gap junction and Z line‑like structures. The present study successfully constructed engineered myocardial tissues in vitro with a PLGA polymer and cardiomyocyte‑like cells derived from BMMSCs, which are likely to share various structural similarities with the original heart tissue.
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