OBJECTIVE To investigate the changes and significance of cell apoptosis, Fas/FasL and P53 protein in epithelial cells from patients with idiopathic pulmonary fibrosis (IPF). METHODS Cell apoptosis and the expressions of Fas/FasL and P53 protein in lung tissues from 12 patients with IPF (IPF group) and 10 normal controls (control group) were detected by terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling (TUNEL) and immunohistochemistry. RESULTS Compared with the control group (0/10), the percentage of apoptosis in alveolar epithelial cells and bronchial cells of the IPF group (12/12) was higher. The percentage of Fas, FasL and P53 protein expressions (12/12, 12/12, 11/12) in alveolar epithelial cells of the IPF group were higher than those of the control group (5/10, 2/10, 0/10); and the percentage of Fas, FasL and P53 protein expressions (12/12, 12/12, 11/12) in bronchial cells of the IPF group were also higher than those of the control group (6/10, 3/10, 0/10). There was a significant correlation between the percentage of apoptosis and Fas/FasL and P53 protein expression (r=0.625-0.839, all P<0.01). The correlation of the Fas/FasL and P53 protein expression was also significant (r=0.571-0.760, all P<0.01). CONCLUSION The apoptosis percentage of epithelial cells and the expression of Fas/FasL and P53 protein are up-regulated in lung tissues of IPF, which may play an important role in the development of the disease.
Objective To explore the value of exhaled nitric oxide detection to predicting the effect of inhaled corticosteroid on chronic obstructive pulmonary disease.Methods After eluting without inhaled corticosteroid before the test,31 ex-smokers with severe chronic obstructive pulmonary disease(mean FEV150% of predicted) were administrated fluticasone propionate 500 μg twice a day for 4 weeks,and were measured fraction of exhaled nitric oxide before the test,and pulmonary function before and after the test.Results The baseline fraction of exhaled nitric oxide was not correlated with FEV1 responses after inhaled corticosteroid.Inhaled corticosteroid responders(increased FEV1≥200 mL had significantly higher baseline fraction of exhaled nitric oxide levels compared with non-responders(P=0.028).The AUC for fraction of exhaled nitric oxide to discriminate responders from non-responders had an area under curve of 0.767.Conclusion In ex-smokers with severe chronic obstructive pulmonary disease,fraction of exhaled nitric oxide is a good indicator to predict the effect of inhaled corticosteroid.
The natural history of idipathic pulmonary fibrosis(IPF)has been characterized as a steady,predictable decline in lung function over time.Recent evidence suggests that some patients may experience a more precipitous course,with periods of relative stability followed by acute deteriorations in respiratory status.Many of these acute deteriorations are of unknown etiology and have termed acute deteriorations of IPF.This article reviews the current progresses in the definition,pathophysiology,etiology,diagnosis,treatment and prophylaxis of acute deteriorations of IPF.For above questions,some have been proved.But still other problems remain disputable.
Key words:
Acute exacerbation; Pulmonary fibrosis; Diagnosis; Definition
OBJECTIVE To evaluate the effects of disinfection and isolation measures on hospital-acquired infection and control and prevention for SARS. METHODS Based on the problems of hospital-acquired infection control and prevention for SARS in Henan Province, the prevention measures including intensive training, standardized precautions, and management were strengthened in all hospitals in Henan. RESULTS There were no any infection for health care workers and no spreading of SARS cases in Henan, no exporting cases from Henan, and no any death cases. CONCLUSIONS The hospital-acquired infection of SARS can be prevented and controlled by means of the effective measures such as disinfection and isolation.
Objective To study the relationship between PI3K、α - SMA protein expression and idiopathic pulmonary fibrosis. Methods 19 lung tissues were collected by open lung biopsies from patients with IPF;the control lung specimens were obtained from 18 patients undergoing thoracotomy for lung disease. Patients in the control group had no other underlying lung pathology and 5 centimeters away from the diseased region. Results The levels of the expression of PI3K、a -SMA protein were significantly higher in the IPF patients than in the control group (respectively U = 25. 000, P < 0. 01 ; U = 9.000, P < 0.01). There was positive correlation of the expression of α - SMA and PI3K protein (r =0. 823, P <0.01). Conclusion The acquisition of an apoptosis - resistant phenotype of myofibroblasts through activation of PI3K - AKT, which participate in the lung injury/repair process, may contribute to persistent or progressive lung fibrosis.
Key words:
Idiopathic pulmonary fibrosis; Phosphatidylinositol - 3 - kinase ; α - Smooth muscle actin; Immunohistochemistry
Objective To investigate the changes and significance of cell apoptosis,P53,P21,Bax and Bcl-2 protein in epithelial cells from patients with idiopathic pulmonary fibrosis (IPF). Methods Cell apoptosis and the expressions of P53,P21,Bax and Bcl-2 protein in lung tissues from 12 patients with IPF (IPF group) and 10 normal controls (control group) were detected by TUNEL(terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling) and immunohistochemistry. Results Compared with that of control group,the percentage of apoptosis in alveolar epithelial cells and bronchial cells of IPF group was higher (all U = 0.0, all P＜0.01). The expressions of P53, P21, Bax protein in alveolar epithelial cells of IPF group were higher than those of control group( U = 5.0,25.5,27.0, all P＜0.05); and the expressions in bronchial cells of IPF group were also higher than those of control group( U =5.0,35.5,25.0,all P＜0.01). The expressions of Bcl-2 protein in alveolar epithelial cells and bronchial cells of IPF group were higher than those of control group( U = 12.0,18.0, all P＜0.01 ). There was a significant correlation between the percentage of apoptosis and P53, P21, Bax protein expression ( rs =0. 436-0. 806 ,all P＜0.01). The correlation of the percentage of apoptosis and Bcl-2 protein expression was also significant (rs=0. 715--0. 699,all P＜0.01). Conclusions The apoptosis percentage of epithelial cells and the expression of P53, P21, Bax protein are up-regulated in lung tissues of IPF, the expression of Bcl-2 protein is down-regulated,which may play an important role in the development of the disease.
Key words:
Pulmonary fibrosis; Apoptosis; Epithelial celIs; Protein P53
Objective To study the relationship of glutathione S-transferase M1 (GSTM1) polymorphisms with occurrence of bronchic lung cancer. Methods The case-control study and PCR-RFLP was conducted among 103 subjects and 138 healthy controls, and the genetic polymorphism of GSTM1 were detected. Logistic regression analysis were used to assess the value of odds ratios (OR) and 95% confidential intervals (CI) between different genotypes and smoking after adjusted by ages and gender. Results For GSTM1, the allele frequencies of deletion(D) were 44.2% and 61.2% in control and LC groups, respectively. The adjusted OR of the GSTM1 deletion(D) genotype showed a 2.09 fold increased risk for LC. The GSTM1 deletion genotypes(D) increased risk for developing SCC and SC, significantly. Conclusion The mutant genotypes GSTM1 deletion polymorphisms raised risk of LC in the population. It is reasonable that GSTM1 deletion genotypes(D) increased risk for developing SCC and SC, obviously. The two genetic polymorphisms and smoking have the joint effect.
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