The aim was to establish a method of detecting tuberculosis infection by enzyme-linked immunospot(ELISPOT)assay and evaluate the application value of CFP10/ESAT6 fusion protein for diagnosis of tuberculosis(TB).By comparing with the different experimental environment,the optimal detecting condition were identified including cell concentration,incubation period and protein antigen concentration,and ELISPOT assay was established.PPD skin test was set as control.It was found that the most appropriate conditions of ELISPOT assay were,2×105 cell/each well,20-40μg/mL CFP10/ESAT6 fusion protein as stimulus and 20 hours of incubation period.The positive rates of PPD skin test in 30 healthy persons,38 patients with non-TB diseases and 40 TB patients were 40%,0% and 72.5%,respectively.Whereas the positive rates of ELISPOT assay were 20.0%,10.5% and 92.5%,respectively.For the 40 TB patients,the sensitivities of ELISPOT in sputum-positive TB group and sputum-negative TB group were 94.4% and 90.9%,respectively.It's concluded that ELISPOT could be used to detect tuberculosis infection efficiently and it might be an important assistant method in diagnosis of tuberculosis.
This thesis studies the beginning and the development of sports analysis the particularity of Chinese culture and the character of Chinese traditional fork sports, expatiate the positive effect of the sports to Chinese culture. So that we can understand the accelerative effect caused by sports to Chinese culture and society clearly.
In Ningwu dialect adverbKeuse frequency is very high,as a modal adverbs,in addition to the general statements,exclamatory sentences,imperative,interrogative sentences and sentence said in a tone are emphasized,also with thejust,justin mandarin, refers to the objective conditions consistent with actual needs; Saidyesand degree adverb,deepening or extent lighter and weaker significance.
Objective To develop a new,rapid and easy method for species identification of Mycobacterium tuberculosis complex(MTBC).Methods Seven sets of primers(Mtbc1-7) for MTBC were used to amplify the genomic DNA from the reference strains and 96 clinical isolates.Results The specificities of 7 sets of primers were identified by PCR with 3 MTBC reference strains,9 non-mycobacterium reference strains and 27 non-tuberculosis mycobacterium(NTM) reference strains. The results showed that mycobacterium and non-mycobacterium were identified by Mtbc1;NTM and MTBC by Mtbc2;M.microti by Mtbc3;M.bovis and M.bovis BCG by Mtbc4;M.africanum subtypeI and M.tuberculosis by Mtbc5;M.bovis BCG by Mtbc4 and Mtbc6 together;M.canettii by Mtbc5 and Mtbc7 together.Of 96 clinical isolates,20 MOTT,3 M.microti,1 M.bovis,1 M.bovis BCG,3 M.africanum subtypeⅠ,1 M.caprae,3 M.canettii,64 M.africanum subtypeⅡ and M.tuberculosis were identified.Conclusion PCR is a rapid and effective method for the species identification of MTBC,and can be used for molecular epidemiology.
Modeling for safety management information systems based on the theory of generative programming and domain engineering,this paper introduces a simple domain modeling language GML,and designs a generator parsing GML documents and creating the destination system.
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