The traditional single minimum support data mining algorithm has some problems, such as too much space occupied by data, resulting in insufficient accuracy of the algorithm, which is difficult to meet the needs of the development of the times. Therefore, an intrusion data mining algorithm based on multiple minimum support is proposed. First, the feature parameters of frequent itemsets of intrusion data are extracted, and the sequence itemsets are divided according to the feature parameters. Then, the data mining features are transformed with the equivalent binary data transformation method, and the multi-support tree structure is optimized according to the data processing results. Data classification mining is carried out with the data tree structure information, and the intrusion data features are deeply mined. Finally, the research of the intrusion data mining algorithm based on the multi-minimum support is completed. Through comparative experiments, it is proved that the accuracy of the intrusion data mining algorithm based on multiple minimum support is 35 % - 75 % higher than that of the traditional single minimum support data mining algorithm.
Abstract Background： Spinal cord injury (SCI) remain one of the great clinical challenges because of their considerable long-term disability potential. In this study, A branched amphiphilic peptide (B-PA) segments were constructed and self-assembled into hydrogels to support bone marrow derived mesenchymal stromal cells (BMSCs) encoding Recombinant Rat VEGF165（VEGF165）, neurotrophins-3(NT-3)and angiopoietin-1(ANG-1) genes. In addition, B-PAS promoted the transdifferentiation of BMSCs into nerve and vascular endothelial cells. Methods： Self-assembly of b-PA into a nanofiber hydrogel was triggered by the culture medium and analysed by transmission electron microscopy (TEM). Rat BMSCs were cultivated using the differential adhesion method and assessed by flow cytometry. Cells were inoculated into the hydrogel and investigated using calcein-AM/PI, CCK-8, and cell adhesion assays. Cells transfected with adenoviral vectors of biocarrier (Adv-Bic) and adenoviral vectors of NT-3 gene (AdvNT-3) were cultured for 7 days and evaluated by immunofluorescence and real time quantitative reverse transcription-PCR. Results： The molecular weight and purity of b-PA were 2191.72 and >95%, respectively. Self-assembled nanofibers had a diameter of 5-8 nm and a length ranging from hundreds of nanometres to micrometres. The resulting CD29+/44+ cells could differentiate into osteoblasts and fat cells, and the hydrogel supported cell growth, proliferation and adhesion. Cells in the hydrogel overexpressed VEGF 165 , NT-3 and Ang-1, and NSE and Nestin markers of CD31+/34+ cells were also abundant. Conclusions： The hydrogel has potential as a tissue engineering scaffold with good cytocompatibility, and can induce genetically modified BMSCs to transdifferentiate into neural and endothelial cell (EC)-like cells. The article have selected preprint on the research platform, this article has been assigned a DOI, The DOI is:10.21203/rs.3.rs-1822746/v1.
To observe the influence of electroacupuncture (EA) intervention on liver function and retinol binding protein 4 (RBP 4) in nonalcoholic fatty liver disease (NAFLD) rats, so as to study its mechanisms underlying improving liver function.A total of 44 SD rats were randomly allocated to normal control, model, EA and non-acupoint groups, with 11 rats in each group. NAFLD model was established by feeding the rats with high glucose and high fat forage for 5 weeks. EA (2 Hz, 1.5 V, 15 min) was applied to unilateral "Fenglong" (ST 40), "Zusanli" (ST 36), "Sanyinjiao" (SP 6) and "Taichong" (LR 3), once daily for 4 weeks. Serum RBP 4 content was assayed by enzyme-linked immunosorbent assay (ELISA), and serum glutamic-oxaloacetic transaminase (AST), alanine aminotransferase (ALT), total cholesterol (TC), and triglycerides (TG) contents were detected by chromatometry.Compared with the normal control group, serum AST, ALT, TC, TG and RBP 4 contents in the model group were increased significantly (P<0. 01), while in comparison with the model group, the levels of the abovementioned 5 serum indexes were down-regulated considerably in the EA group (P < 0.05) rather than in the non-acupoint group (P > 0.05).EA intervention can lower serum AST, ALT, TC, TG and RBP 4 contents in NAFLD rats, which may be responsible for its action in improving NAFLD in clinic.
To explore the application of the neobladder-urethral drag-and-bond anastomosis technique in laparoscopic radical cystectomy (LRC) with ileal orthotopic neobladder (IONB) reconstruction.This is a retrospective cohort study on a procedure performed by a single surgeon. From January 2014 to December 2018, we identified 43 male bladder cancer patients who received LRC with IONB reconstruction. These patients were divided into two groups, with 22 patients undergoing neobladder-urethral drag-and-bond anastomosis (NUDA) and 21 patients undergoing neobladder-urethral anastomosis under laparoscopy (NUAL). Anastomosis time, catheter removal time, postvoid residual (PVR), maximum urinary flow rate (Q-max), urine leakage and anastomotic stenosis were used to evaluate the simplicity and surgical effect of the two groups.Both groups demonstrated similar tumor characteristics. A significant difference in neobladder-urethral anastomosis time was found between the NUDA group and the NUAL group (14.6 ± 0.4 vs 70 ± 2.5 min, P<0.0001), and there was no significant difference in other characteristics.The neobladder-urethral drag-and-bond anastomosis technique in LRC and IONB reconstruction, with its shorter learning curve, was easier and more convenient than neobladder-urethral anastomosis under laparoscopy.
• There are various complications in ureteral stenting, but perforation of renal parenchyma is rare. • Analyze factors of complications and provide treatment options. • Summarize the treatment of all literature to provide a useful reference experience for clinical work.
Abstract Increasing reports indicate that circular RNAs (circRNAs) are very important regulators in human diseases, including cancers. In bladder cancer (BC), several circRNAs have been reported to be involved in tumor progressions, such as circ‐ITCH and circTCF25. However, the functions of most circRNAs in BC still remains largely unknown. In this study, we identified a novel circRNA termed as circ‐VANGL1 by bioinformatics analysis. We found that circ‐VANGL1 was highly expressed in BC tissues compared with adjacent normal tissues. Furthermore, we showed that circ‐VANGL1 could serve as a prognostic marker for patients with BC. Through functional experiments, we found that circ‐VANGL1 knockdown significantly suppressed BC cell proliferation, cell cycle, migration, and invasion in vitro. Besides, circ‐VANGL1 silence inhibited BC cell propagation in vivo. Mechanistically, we identified circ‐VANGL1 as a sponge of miR‐605‐3p which targeted VANGL1 in BC cells. Through repressing miR‐605‐3p availability, circ‐VANGL1 contributes to VANGL1 expression, consequently leading to BC cell proliferation, migration, and invasion. Taken together, our study demonstrated circ‐VANGL1/miR‐605‐3p/VANGL1 as a novel essential signaling pathway involved in BC progression.
The TCM acupuncture education for foreign students is complicated and technical work.The writer discusses the modern teaching methods recently to aim directly at the problems in the traditional teaching methods in foreign students education,analyzes and compares the connotation and teaching mode of these teaching methods,and at last puts forward that we should choose correct teaching method according to the actuality to improve the teaching quality for foreign students in TCM acupuncture.
Key words:
Foreign students; Acupuncture; English teaching
The Accepted Manuscript version of this article (published on 22 June 2018) was withdrawn on 9 April 2021 at the request of the authors. Despite numerous attempts to contact the authors, the Editorial Office have been unable to receive a reason behind the withdrawal request.
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