Guoming Qi

National Institute for Communicable Disease Control and Prevention

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Biao Kan

National Institute for Communicable Disease Control and Prevention

Yanqing Liu

Columbia University Irving Medical Center

Shouyi Gao

Iwate University

Gao Shouyi

NARI Group (China)

Lijuan Zhang

Tennessee State University

Guangwen Liu

Peking University

Meiying Yan

National Institute for Communicable Disease Control and Prevention

Weili Liang

Hebei Agricultural University

Yongyu Rui

Southern Medical University

Fenggang Yu

National University of Singapore

Cooperative Institutions

Chinese Center For Disease Control and Prevention

National Institute for Communicable Disease Control and Prevention

Chinese Academy of Medical Sciences & Peking Union Medical College

Center for Disease Control

State Key Laboratory of Infectious Disease Prevention and Control

Peking University

Chinese Academy of Sciences

Sun Yat-sen University

Shandong University

Capital Medical University

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Construction and Characterization of a <I>thyA</I> Mutant Derived From Cholera Vaccine Candidate IEM101

Molecular Biotechnology (2005)

Fenggang Yu Guoming Qi Yanqing Liu Shouyi Gao Biao Kan

Cholera

10.1385/mb:29:3:191

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Citations (4)

[Study on infection of different strains of Vibro cholerae O1 by El Tor CTXPhi].

PubMed (2005)

Guangwen Liu Meiying Yan Guoming Qi Shouyi Gao Biao Kan

To study the horizontal transfer efficiencies of filamentous bacteriophage CTXPhi in different V. cholera O1 strains and the phage immunities of these strains. The infectious El Tor CTXPhi particles genetic marked by chloramphenicol resistance gene were used to infect four different V. cholerae O1 strains in vivo and in vitro. Selected the infected clones based on its character of chloramphenicol resistance and identified and judged the exist form of CTXPhi genome through Southern bolt and other hybridization methods. Calculated the infection rates of different strains and compared each other. Then we analyzed the mechanism of infection and phage immunity. The infection rate of classic strain 1119 with the genetic marked CTX(ET)Phi in vivo is much higher than that in vitro. In vitro experiment, the rate of 1119 is higher than other three El Tor strains. And in El Tor strains, the infection frequency of IEM101 that had no rstR gene is 100 to 1000 times higher than other two strains containing rstR. Classical biotype strain is more susceptible to CTX(ET)Phi particles than El Tor strains. Expression of TCP and the phage immunity mediated by rstR gene affect the horizontal transfection of CTXPhi in V. cholerae strains.

El Tor

Strain (injury)

Cholera

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Complete genome sequence and analysis of {\sl Vibrio cholerae} phage VP2

Bingdu xuebao (2005)

Duochun Wang Min Wang Yanping Li Hui Dong Zhonghua Liu

The results showed that VP2 belongs to Podoviridae, the circular double-stranded DNA (ds DNA) VP2 genome consists of 29853 bp, with overcall G+C content of 50.56%, which was higher than the G+C content of N16961, a sequenced strain of Vibrio cholerae. Putative forty-five open reading frames (ORF) were annotated, homology analysis were used to assign possible functions for the genes, including, DNA replication, synthesis of capsid protein, DNA packaged genes, and infection-associated genes. The result of phylogenetic tree of DNA pol showed VP2 was clustered together with phage Cp-1 and GA-1. The evolutive analysis above suggests VP2 belongs to phi29-like phage.

Podoviridae

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Resistance of the cholera vaccine candidate IEM108 against CTXΦ infection

Vaccine (2005)

Guangwen Liu Meiying Yan Weili Liang Guoming Qi Yanqing Liu

Cholera toxin

El Tor

Cholera

Cholera vaccine

Lysogenic cycle

10.1016/j.vaccine.2005.09.059

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Citations (12)

Establishment of fluorescent amplified fragment length polymorphism in Vibrio cholerae and evaluation in molecular typing

Jing Lou Baowei Diao Hongxia Wang Zhigang Cui Guoming Qi

OBJECTIVE To develop fluorescent amplified fragment length polymorphism (AFLP) method and to evaluate the its typing capability with pulsed-field gel electrophoresis (PFGE) in molecular typing of Vibrio cholerae. METHODS Forty-seven strains of V. cholerae, with different PFGE patterns, were selected as the reference group to optimize the selective primers of AFLP analysis. Eighty-three strains including 20 strains from one epidemic episode, isolated from different provinces during 1961 and 2005, were used to compare the typing abilities of AFLP and PFGE. LI-COR4300 DNA sequencing system was used for AFLP electrophoresis. The images were recorded by Saga(MX) software and transferred to BioNumerics for clustering analysis. A standard protocol for V. cholerae from PulseNet was used in PFGE. RESULTS When comparison was made with different selective primers on AFLP based on the 47 strains, results showed that the optimized selective primer pair was EcoR I-G/Mse I-T, and the reproducibility of the tests was 99.2%. Eighty-three isolates showed 52 AFLP patterns and 44 PFGE patterns, with D values as 0.9545 (AFLP) and 0.9251 (PFGE) respectively. CONCLUSION The protocol of fluorescent AFLP on V. cholerae typing was established. AFLP was higher than PFGE in discrimination of V. cholerae which could be used for molecular typing. When combined with PFGE, AFLP became a more insightful tool to identify genome difference of different isolates.

DNA profiling

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Citations (1)

Making Great Efforts to Promote the Development and Application of Evidence-Based Medicine (English version)

Zhongguo xunzheng yixue zazhi (2005)

Guoming Qi

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[A study on genetic polymorphism of rRNA gene pattern of Vibrio cholerae O139 in China].

PubMed (2002)

Mei Qu Biao Kan Guoming Qi Yanqing Liu Shouyi Gao

To investigated the genetic polymorphism of the isolated strains using ribotyping method.One hundred twenty-two strains of V. cholerae O139 isolated from different areas of China from 1993 to 1999 were selected and characterized with ribotyping, including 16s rDNA and 23s rDNA probes.One hundred twenty-two strains were differentiated into 10 different ribotypes (RT1-RT10) on the basis of rRNA gene probes hybridization (with Bgl I digestion), which consisted of 7 - 9 bands between 12 and 1.5 kb in size. RT1 and RT3, as two predominant ribotypes, comprised most number of the strains which spread to the extensive range. Nine strains, which are negative to ctxAB, zot and RS individually, belong to 4 special ribotypes. The dendrogram revealing genetic relationship among different clones of V. cholerae O139 showed that the clones belonging to RT1 and RT2 had genetic similarity on high degree, although they were isolated from different regions. The two predominant ribotypes (RT1 and RT3) were distant in genetic relationship.Results showed the clonal diversity and the wide area distribution of V. cholerae O139 strains in China, suggesting the multiple origins of O139 epidemics.

Ribotyping

Dendrogram

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Study of measure method for true course of submarine

Guoming Qi

Measure method for true course of submarine is a difficult problem that had not been solved in our nation. Target of our study is to seek a measure method of navigation that is higher precise compared with that of ships. Higher precise measure system of inertia navigation based on small type inertia navigation system suffices measure requests of true course of submarine various state.

Course (navigation)

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Construction and Evaluation of a Safe, Live, Oral Vibrio cholerae Vaccine Candidate, IEM108

Infection and Immunity (2003)

Weili Liang Shixia Wang Fenggang Yu Lijuan Zhang Guoming Qi

IEM101, a Vibrio cholerae O1 El Tor Ogawa strain naturally deficient in CTXPhi, was previously selected as a live cholera vaccine candidate. To make a better and safer vaccine that can induce protective immunity against both the bacteria and cholera toxin (CT), a new vaccine candidate, IEM108, was constructed by introducing a ctxB gene and an El Tor-derived rstR gene into IEM101. The ctxB gene codes for the protective antigen CTB subunit, and the rstR gene mediates phage immunity. The stable expression of the two genes was managed by a chromosome-plasmid lethal balanced system based on the housekeeping gene thyA. Immunization studies indicate that IEM108 generates good immune responses against both the bacteria and CT. After a single-dose intraintestinal vaccination with 10(9) CFU of IEM108, both anti-CTB immunoglobulin G and vibriocidal antibodies were detected in the immunized-rabbit sera. However, only vibriocidal antibodies are detected in rabbits immunized with IEM101. In addition, IEM108 but not IEM101 conferred full protection against the challenges of four wild-type toxigenic strains of V. cholerae O1 and 4 micro g of CT protein in a rabbit model. By introducing the rstR gene, the frequency of conjugative transfer of a recombinant El Tor-derived RS2 suicidal plasmid to IEM108 was decreased 100-fold compared to that for IEM101. This indicated that the El Tor-derived rstR cloned in IEM108 was fully functional and could effectively inhibit the El Tor-derived CTXPhi from infecting IEM108. Our results demonstrate that IEM108 is an efficient and safe live oral cholera vaccine candidate that induces antibacterial and antitoxic immunity and CTXPhi phage immunity.

Cholera toxin

El Tor

Cholera vaccine

Cholera

Humoral immunity

10.1128/iai.71.10.5498-5504.2003

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Citations (98)

IEM101, a naturally attenuated Vibrio cholerae strain as carrier for genetically detoxified derivatives of cholera toxin

Vaccine (2000)

Maria Rita Fontana Elisabetta Monaci Yanqing Liu Guoming Qi Guangcai Duan

Cholera toxin