Arisa Kubota

University of Fukui

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Shuji Watanabe

University of Fukui

Akihiko Matsumine

University of Fukui

Hideaki Nakajima

University of Fukui

Kazuya Honjoh

University of Fukui

Ding Li

Nanjing Medical University

Takashi Tonozuka

Tokyo University of Agriculture and Technology

Naoto Takeura

University of Fukui

Takumi Tochio

Fujita Health University

Atsushi Nishikawa

Osaka University

Nobumitsu Sasaki

Tokyo University of Agriculture and Technology

Cooperative Institutions

Institute for Conflict Research

Research Organization of Information and Systems

Nagoya University

Tokyo University of Agriculture and Technology

Kitasato University

University of Fukui

Level (Czechia)

Kyoto University

Open Evidence

Keio University

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Surgical strategy for metastatic spinal tumors based on Spine Instability Neoplastic Score and patient-reported outcomes: JASA multicenter prospective study

Journal of Neurosurgery Spine (2024)

Hideaki Nakajima Shuji Watanabe Kazuya Honjoh Arisa Kubota Yuki Shiratani

Instrumentation surgery in combination with radiotherapy (RT) is one of the key management strategies for patients with spinal metastases. However, the use of materials can affect the RT dose delivered to the tumor site and surrounding tissues, as well as hinder optimal postoperative tumor evaluation. The association of the preoperative Spine Instability Neoplastic Score (SINS) with the need for spinal stabilization and life expectancy are unclear. This multicenter prospective study aimed to investigate the current situation and make recommendations regarding the choice of surgical procedure based on the preoperative SINS and prospectively collected postoperative patient-reported outcomes (PROs). The study prospectively included 317 patients with spinal metastases who underwent palliative surgery and had a minimum follow-up period of 6 months. The survey items included SINS, patient background, and clinical data including surgical procedure, history of RT, prognosis, and PROs (i.e., the visual analog scale score, Faces Scale, Barthel Index, Vitality Index, and 5-level EQ-5D health survey) at baseline, and at 1 and 6 months after surgery. The association of preoperative SINS with life expectancy, PROs, and surgical procedures was examined using statistical analysis. Preoperative SINS (three categories) had no association with life expectancy. All PROs evaluated in the study improved up to 6 months after surgery. Pain categories (visual analog scale score and/or Faces Scale) at baseline were correlated with preoperative SINS. As many as 90.9% of enrolled patients underwent fusion surgery, and even in SINS 0-6 cases, implants were used in 64.3% of patients. Postoperative RT was performed in 42.9% of the patients. However, prospective assessments of PROs showed no significant difference between surgical procedures (with and without fusion) in patients with SINS 0-9. In addition, no cases required conversion from noninstrumentation surgery to fusion surgery. Although the choice of surgical procedure should be made on a case-by-case basis on the NOMS (neurological, oncological, mechanical, and systemic) framework, careful consideration is required to determine whether spinal stabilization is needed in patients with SINS ≤ 9, considering the patient's background and the plan for postoperative adjuvant therapy.

10.3171/2024.7.spine24340

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Characterization and alteration of product specificity of Beijerinckia indica subsp. indica β-fructosyltransferase

Bioscience Biotechnology and Biochemistry (2023)

Ding Li Yuki Miyasaka Arisa Kubota Takuma Kozono Yoshikazu Kitano

The trisaccharide 1-kestose, a major constituent of fructooligosaccharide, has strong prebiotic effects. We used high-performance liquid chromatography and 1H nuclear magnetic resonance spectroscopy to show that BiBftA, a β-fructosyltransferase belonging to glycoside hydrolase family 68, from Beijerinckia indica subsp. indica catalyzes transfructosylation of sucrose to produce mostly 1-kestose and levan polysaccharides. We substituted His395 and Phe473 in BiBftA with Arg and Tyr, respectively, and analyzed the reactions of the mutant enzymes with 180 g/L sucrose. The ratio of the molar concentrations of glucose and 1-kestose in the reaction mixture with wild-type BiBftA was 100:8.1, whereas that in the reaction mixture with the variant H395R/F473Y was 100:45.5, indicating that H395R/F473Y predominantly accumulated 1-kestose from sucrose. The X-ray crystal structure of H395R/F473Y suggests that its catalytic pocket is unfavorable for binding of sucrose while favorable for transfructosylation.

Trisaccharide

Oligosaccharide

Fructan

Fructooligosaccharide

10.1093/bbb/zbad074

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Citations (1)

Adjustment-free LSI chip-set for CD player application

IEEE Transactions on Consumer Electronics (1995)

M. Arai H. Arai Arisa Kubota Tadashi Sumi

The authors have developed an adjustment-free LSI chip-set for CD player application. This chip set contains an automatic gain control function with which a low modulation amplitude disc is playable. Analog/digital mixed technology is used in this chip-set.< >

Chipset

10.1109/30.468079

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Citations (3)

P19. Risk factors for postoperative bladder dysfunction in lumbar spinal canal stenosis surgery

North American Spine Society Journal (NASSJ) (2024)

Hideaki Nakajima Shuji Watanabe Kazuya Honjoh Arisa Kubota Akihiko Matsumine

Spinal Surgery

10.1016/j.xnsj.2024.100423

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Adipose-Derived Mesenchymal Stromal Cell Transplantation for Severe Spinal Cord Injury: Functional Improvement Supported by Angiogenesis and Neuroprotection

Cells (2023)

Ai Takahashi Hideaki Nakajima Arisa Kubota Shuji Watanabe Akihiko Matsumine

Mesenchymal stromal cell transplantation alone is insufficient when motor dysfunction is severe; combination therapy with rehabilitation could improve motor function. Here, we aimed to analyze the characteristics of adipose-derived MSCs (AD-MSCs) and determine their effectiveness in severe spinal cord injury (SCI) treatment. A severe SCI model was created and motor function were compared. The rats were divided into AD-MSC-transplanted treadmill exercise-combined (AD-Ex), AD-MSC-transplanted non-exercise (AD-noEx), PBS-injected exercise (PBS-Ex), and no PBS-injected exercise (PBS-noEx) groups. In cultured cell experiments, AD-MSCs were subjected to oxidative stress, and the effects on the extracellular secretion of AD-MSCs were investigated using multiplex flow cytometry. We assessed angiogenesis and macrophage accumulation in the acute phase. Spinal cavity or scar size and axonal preservation were assessed histologically in the subacute phase. Significant motor function improvement was observed in the AD-Ex group. Vascular endothelial growth factor and C-C motif chemokine 2 expression in AD-MSC culture supernatants increased under oxidative stress. Enhanced angiogenesis and decreased macrophage accumulation were observed at 2 weeks post-transplantation, whereas spinal cord cavity or scar size and axonal preservation were observed at 4 weeks. Overall, AD-MSC transplantation combined with treadmill exercise training improved motor function in severe SCI. AD-MSC transplantation promoted angiogenesis and neuroprotection.

10.3390/cells12111470

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Citations (8)

Risk Factors for de Novo Postoperative Urinary Retention in Posterior Lumbar Spine Surgery

Global Spine Journal (2024)

Hideaki Nakajima Kazuya Honjoh Shuji Watanabe Naoto Takeura Arisa Kubota

Retrospective cohort study.

Urinary retention

10.1177/21925682241308508

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Relationship Between Vertebral Bone Marrow Edema and Early Progression of Intervertebral Disc Wedge or Narrowing After Lumbar Decompression Surgery

Spine (2021)

Hideaki Nakajima Kazuya Honjoh Shuji Watanabe Arisa Kubota Akihiko Matsumine

A retrospective study.The aim of this study was to review clinical and imaging features in patients with lumbar spinal canal stenosis (LSS) with and without postoperative early progression of intervertebral disc degeneration (IDD) and to identify predictive factors.Progression of IDD after lumbar decompression surgery can induce low back pain and leg pain, and may require revision surgery. However, risk factors for postoperative radiological changes indicating IDD linked to development of symptoms have not been described.We included 564 patients with LSS without degenerative lumbar scoliosis who underwent lumbar decompression surgery without fusion. Clinical features and imaging findings were compared in cases with (group P) and without (group N) progression of IDD (intervertebral disc wedge or narrowing) at 1 year after surgery.Of the 564 patients, 49 (8.7%) were in group P. On preoperative MRI, all patients in group P had findings of vertebral bone marrow edema (diffuse high intensity on T2-weighted images and low-intensity on T1-weighted images), compared to only 5.4% in group N. The rate of revision surgery was significantly higher in group P in 5 years' follow-up (12.2% vs. 1.4%, P < 0.01). In group P, 44.9% of patients developed postoperative symptoms associated with postoperative radiological changes, and the frequency was higher in narrowing-type than in wedge-type cases. Vertebral bone marrow edema area and IDD grade were not predictors for postoperative early IDD progression.Careful consideration is required to determine whether lumbar decompression surgery should be performed if vertebral bone marrow edema is detected on MRI, since this is a predictor for a negative clinical outcome. If surgery is symptomati-cally urgent, careful clinical and radiological follow-up is required.Level of Evidence: 4.

10.1097/brs.0000000000004108

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Citations (3)

Clinical impact of short limited lumbar fusion for adult spinal deformity with postural and radiological abnormalities

Scientific Reports (2022)

Hideaki Nakajima Hideaki Matsuo Hiroaki Naruse Shuji Watanabe Kazuya Honjoh

Extensive surgical spinopelvic fusion for patients with adult spinal deformity (ASD) to achieve optimal radiological parameters should be avoided. The aim of this study was to review clinical and imaging findings in patients with ASD with postural and radiological abnormalities who underwent a novel three-level limited lumbar fusion as two-stage surgery in an attempt to propose a better tolerated alternative to spinopelvic long fusion to the pelvis. The subjects were 26 patients with a minimum follow-up period of 2 years. Cobb angle, C7 sagittal vertical axis, and pelvic incidence (PI) minus lumbar lordosis (LL) were significantly improved after surgery and maintained at follow-up. Most radiological parameters were corrected with lateral interbody fusion (LIF) as the initial surgery, and few with posterior fusion. PI-LL remained high after limited lumbar fusion, but scores on patient-based questionnaires and sagittal and coronal tilt in gait analysis improved. In cases with postoperative progression of proximal junctional kyphosis (11.5%), residual L1-L2 local kyphosis after LIF was the most significant radiological feature. In some cases of ASD with postural abnormalities, short limited lumbar fusion gives sufficient postoperative clinical improvement with preservation of spinal mobility and activities of daily living. The range of fixation should be determined based on radiological parameters after LIF to avoid postoperative complications.

Pelvic tilt

Kyphosis

Spinal disease

10.1038/s41598-022-23933-z

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Citations (2)

Enzymatic and structural characterization of β-fructofuranosidase from the honeybee gut bacterium Frischella perrara

Applied Microbiology and Biotechnology (2022)

Arisa Kubota Reika Kawai Ding Li Takuma Kozono Nobumitsu Sasaki

Trisaccharide

Tetrasaccharide

Disaccharide

Hydrolase

10.1007/s00253-022-11863-9

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Pretreatment Prognostic Factors for Intradiscal Condoliase Injection in Patients with Lumbar Disc Herniation: Insights from Clinical and MRI-Based Quantitative Analysis

Journal of Clinical Medicine (2025)

Hideaki Nakajima Arisa Kubota Shuji Watanabe Kazuya Honjoh Naoto Takeura

Background/Objectives: Intradiscal condoliase injection is a minimally invasive and effective treatment option for lumbar disc herniation (LDH). However, the appropriate use, efficacy, and potential outcomes of this therapy have to be carefully considered because condoliase can only be administered once in life. The aim of this study is to identify factors that predict the efficacy of condoliase injection before treatment. Methods: A retrospective analysis was performed for 115 patients with LDH treated with intradiscal condoliase injection. The patients’ background and MRI-based evaluations were used to measure various pretreatment parameters, including age, sex, symptom duration, comorbidity of psychological factors, disc height, herniated mass area, and contrast ratios within the disc and herniation. Clinical response was defined as a ≥50% reduction in leg pain on a numerical rating scale 3 months after treatment. Factors with significance in the univariate analysis were further examined using multivariate logistic regression. Results: Among the 115 patients, 73.9% had a ≥50% post-treatment pain reduction. The predictive factors for poorer outcomes included a longer symptom duration, psychological comorbidities, a smaller herniated mass size, a higher disc height, and a higher contrast ratio within the disc. No significant associations were found between the treatment efficacy and patient age or contrast ratio within the herniation. Conclusions: This study identified several pre-treatment factors that predict the efficacy of intradiscal condoliase injection for LDH. Treatment decisions should be made with particular attention to patients with a longer symptom duration, psychological factors, and fewer degenerative discs. The most important determinant of treatment efficacy may be how condoliase acts on the herniation mass.

Lumbar disc herniation

Disc herniation

10.3390/jcm14051509

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