Takashi Yanagisawa

Institute of Crop Science

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Takashi Nagamine

Sapporo Medical University

Noriko Kohyama

Food Research Institute

Seiichiro Aoe

Otsuma Women's University

Kyuya Harada

Osaka University

Hisashi Yoshida

Okayama Prefecture

Yasunori Ichinose

Institute of Crop Science

Chikako Kiribuchi‐Otobe

Institute of Crop Science

Chiemi Yamanaka

Otsuma Women's University

Toji Yoshioka

Institute of Crop Science

Kento Mio

Otsuma Women's University

Cooperative Institutions

National Agriculture and Food Research Organization

Institute of Crop Science

The University of Tokyo

Western Region Agricultural Research Center

Tochigi Prefectural Fisheries Experimental Station

Tohoku University

Okayama University

Institute of Agrobiological Sciences

University of Tsukuba

Central Region Agricultural Research Center

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Ant28 gene for proanthocyanidin synthesis encoding the R2R3 MYB domain protein (Hvmyb10) highly affects grain dormancy in barley

Euphytica (2011)

Eiko Himi Yūko Yamashita Naoto Haruyama Takashi Yanagisawa Masahiko Maekawa

MYB

Candidate gene

10.1007/s10681-011-0552-5

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Citations (49)

Factors of Fluctuation in Glassy Grain Rate and β-Glucan Content and their Control by Fertilizing Technology in Barley Cultivar Shunrai for Barley Rice.

Japanese Journal of Crop Science (2021)

Takeshi Okiyama Takashi Yanagisawa Takashi Nagamine Makoto Oyama Takahiro Sekiwa

食用オオムギにおいて,精麦品質の面から重要となる胚乳の硝子率と,機能性が注目されている穀粒のβ–グルカン含有率について,現地農家圃場のデータから変動要因を解析するとともに,栃木県農業試験場において施肥法による両者の制御に関する試験を行った.まず,栃木県の農家圃場で生産されたシュンライを用いて変動要因を解析した.硝子率とβ–グルカン含有率は穀粒タンパク質含有率と高い相関関係にあることから,タンパク質含有率の制御が高品質生産の鍵になると考えられた.硝子率は年次変動がみられたが,タンパク質含有率8%以下であれば,硝子率許容値である50%以下となる可能性が高まり,そのときのβ–グルカン含有率は4.4%であった.しかし,減肥栽培による低タンパク質含有率化では,収量の変動が大きく,収量安定化と低硝子率の両立は困難であると考えられた.栃木県農業試験場内の試験では,茎立期30日前と茎立期の両時期に追肥する分施体系は,全量基肥栽培に比べてβ–グルカン含有率は低下するものの,収量が向上して硝子率が低下したことから,実用的な施肥方法であると考えられた.また,目標とするタンパク質含有率に収めるための追肥の可否を茎立期30日前の茎数×SPAD値により診断できる可能性が示唆された.β–グルカン含有率を高めるといわれる出穂期10日後の追肥は,硝子率が増加して品質が低下したことから,シュンライにおいては適切な技術ではないと考えられた.

10.1626/jcs.90.194

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Citations (1)

Variation in DNA Fingerprints Detected in Plants Regenerated from Horseradish (Armoracia rusticana Gaertn.) Callus.

Ikushugaku zasshi (1995)

Masae Sugaya Masaru Niwa Kyuya Harada Takashi Yanagisawa Wataru Marubashi

DNA fingerprints patterns were compared among regenerated plants from root- and leaf-derived calli of horseradish using digoxygenin-labeled oligonucleotide probes for Southern hybridization. Among the seven probes investigated, (GAA)5 produced the clearest banding patterns. Two out of 14 regenerated plants from leaf-derived calli and five out of 12 plants from rootderived calli exhibited clearly different patterns from their mother plants. Except for one, these variant plants in DNA fingerprints could not be distinguished morphologically from their mother plants. Thus, DNA finterprinting is considered a possible means for de-tecting somaclonal variation in horseradish.

Somaclonal variation

Callus

10.1270/jsbbs1951.45.195

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Citations (2)

Comparison of [ 18 F]fluorosulfenylation to [ 18 F]fluoroselenenylation.

Journal of Labelled Compounds and Radiopharmaceuticals (1995)

Keiichi Hatano Takashi Yanagisawa

Source

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Analysis of replication pattern in soybean chromosomes by indirect immunofluorescence method.

The Japanese Journal of Genetics (1993)

Takashi Yanagisawa Shigemitsu Tano Kiichi Fukui Kyuya Harada

An indirect immunofluorescence method detecting the incorporation of BrdU was applied to analyze the DNA replication pattern in soybean chromosomes which are very small among plant species so far examined. Discrete fluorescent images in the distal, interstitial and proximal regions of the chromosome arms were revealed at prometaphase. Time-course analysis indicated that the DNA replication starts at the distal region. The replication patterns are discussed in relation to other chromosomal characteristics such as the location of constitutive heterochromatin and the stainability with the Giemsa.

Replication

Prometaphase

Immunofluorescence

Giemsa stain

10.1266/jjg.68.119

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Development of Genome-Wide SNP Markers for Barley via Reference- Based RNA-Seq Analysis

Frontiers in Plant Science (2019)

Tsuyoshi Tanaka Goro Ishikawa Eri Ogiso‐Tanaka Takashi Yanagisawa Kazuhiro Sato

Marker-assisted selection of crop plants requires DNA markers that can distinguish between the closely related strains often used in breeding. The availability of reference genome sequence facilitates the generation of markers, by elucidating the genomic positions of new markers as well as of their neighboring sequences. In 2017, a high quality genome sequence was released for the six-row barley (Hordeum vulgare) cultivar Morex. Here, we developed a de novo RNA-Seq-based genotyping procedure for barley strains used in Japanese breeding programs. Using RNA samples from the seedling shoot, seedling root, and immature flower spike, we mapped next-generation sequencing reads onto the transcribed regions, which correspond to ∼590 Mb of the whole ∼4.8-Gbp reference genome sequence. Using 150 samples from 108 strains, we detected 181,567 SNPs and 45,135 indels located in the 28,939 transcribed regions distributed throughout the Morex genome. We evaluated the quality of this polymorphism detection approach by analyzing 387 RNA-Seq-derived SNPs using amplicon sequencing. More than 85% of the RNA-Seq SNPs were validated using the highly redundant reads from the amplicon sequencing, although half of the indels and multiple-allele loci showed different polymorphisms between the platforms. These results demonstrated that our RNA-Seq-based de novo polymorphism detection system generates genome-wide markers, even in the closely related barley genotypes used in breeding programs.

Indel

Amplicon

RNA-Seq

SNP genotyping

10.3389/fpls.2019.00577

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Citations (23)

Effects of Various Blending Ratios of Rice and Waxy Barley on Postprandial Blood Glucose Levels

Nippon Eiyo Shokuryo Gakkaishi (2018)

Seiichiro Aoe Kôzô Komae Yutaka Inoue Isamu Murata Yuki Minegishi

本研究は, 異なる配合率のモチ性の大麦品種「キラリモチ」混合米飯の摂取が米飯と比較して食後血糖値の上昇を配合率依存的に抑制するかとGlycemic Index (GI) が配合率依存的に低下するか検証するため実施した。対象は健常な成人10名 (男性6名, 女性4名) とした。糖質50 g分の米飯 (基準食) とモチ性大麦の配合率が30, 50, 100%の大麦混合米飯 (試験食) のそれぞれを摂取し, 摂取前および摂取開始から15, 30, 45, 60, 90, 120分後の血糖値を測定した。血糖値, 血糖値上昇曲線下面積 (IAUC) , Glycemic Index (GI) について解析した。大麦の配合率が増加するほど血糖値とIAUC0-120が低下した。GIは, 基準食を100とした場合, 大麦配合率30, 50, 100%でそれぞれ, 79.7, 67.5, 48.3であった。モチ性大麦混合米飯には, β-グルカン量に依存して米飯と比較して血糖値の上昇を抑制する効果が示唆された。

Glycaemic index

10.4327/jsnfs.71.283

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Citations (2)

Interactions Among Wx Genes and Effects of a Mutated Wx‐D1e Allele on Starch Properties in Wheat

Cereal Chemistry (2004)

Chikako Kiribuchi‐Otobe Kanako Kurita Takashi Yanagisawa Genkichi Takeda Atsuo Inazu

ABSTRACT The waxy mutant wheat Tanikei A6599‐4 contains little amylose and exhibits a stable hot paste viscosity. It has null alleles at the Wx‐A1 and Wx‐B1 loci and a mutated allele at the Wx‐D1 locus ( Wx‐D1e ). From the cross‐combination of Kanto 123 (normal amylose line) and Tanikei A6599‐4, 51 DH (doubled haploid) lines were produced and their genotypes were determined by SDS‐PAGE and a derived cleaved amplified polymorphic sequence (dCAPS) analysis. Eight genotypes were obtained at the expected ratio. Using a Rapid Visco Analyser (RVA), all the lines with the same genotype as Tanikei A6599‐4 showed a stable hot paste viscosity. The other lines did not show a stable hot paste viscosity regardless of the presence of the Wx‐D1e allele. When two genotypes with the same Wx‐A1 and Wx‐B1 alleles were compared, the one with Wx‐D1e contained less amylose and exhibited a lower final viscosity and a lower setback with RVA. Although the Wx‐D1e allele produces an almost inactive Wx protein, these findings suggest that this allele contributes at some level to starch synthesis.

Null allele

10.1094/cchem.2004.81.1.48

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Citations (8)

Breeding of a new six-rowed waxy barley cultivar “Kihadamochi” exhibiting high levels of yield and β-glucan content

Breeding Research (2022)

Takuji Tonooka Takashi Yanagisawa Emiko Aoki Masato Taira Toji Yoshioka

10.1270/jsbbr.21j15

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Citations (2)

Effects of barley β‐glucan with various molecular weights partially hydrolyzed by endogenous β‐glucanase on glucose tolerance and lipid metabolism in mice

Cereal Chemistry (2020)

Kento Mio Chiemi Yamanaka Yasunori Ichinose Noriko Kohyama Takashi Yanagisawa

Abstract Background and objectives In this study, we investigated whether the intake of a high‐fat diet containing barley β‐glucan partially hydrolyzed by endogenous β‐glucanase (PHEB) for 2 or 8 hr affects glucose metabolism, lipid metabolism, and intestinal microbiota compared with untreated barley (BF) and a control diet in mice. Findings A significant increase in cecum weight was observed in all barley groups compared with the control group. Liver lipid accumulation was significantly decreased in all barley groups. In mice given barley flour hydrolyzed for 8 hr (PHEB‐8h), blood glucose levels tended to decrease at 30 min when compared with the control group ( p = .06). Total short‐chain fatty acids (SCFA) contents in the cecum were significantly increased in the BF and PHEB groups compared with the control group. Conclusions We demonstrated that the improving effects of barley β‐glucan on glucose and lipid metabolism were maintained even after partial hydrolysis, whereas the effects of barley β‐glucan on intestinal microbiota and SCFA production were moderately changed by partial hydrolyzation. Significance and novelty The findings of this study showed that, even after the cooking process, partially hydrolyzed barley β‐glucan is expected to improve glucose and lipid metabolism, similar to intact barley β‐glucan.

Cecum

Glucanase

Barley flour

Carbohydrate Metabolism

Avena

10.1002/cche.10328

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Citations (17)