Maria de Lourdes Contente Gomes

Ministério da Saúde

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Alexandre da Costa Linhares

Instituto Evandro Chagas

Ana Lúcia Monteiro Wanzeller

Ministério da Saúde

Delana Andreza Melo Bezerra

Instituto Evandro Chagas

Olinda Macêdo

Instituto Evandro Chagas

Ceyla Maria Oeiras de Castro

Instituto Evandro Chagas

Edna da Silveira

Ministério da Saúde

Jainara Cristina dos Santos Alves

Instituto Evandro Chagas

Letícia Martins Lamarão

Fundação Hemopa

Mirleide Cordeiro dos Santos

Instituto Evandro Chagas

Darleise de Souza Oliveira

Instituto Evandro Chagas

Cooperative Institutions

Instituto Evandro Chagas

Ministério da Saúde

Universidade Federal do Pará

Universidade do Estado do Pará

Fundação Oswaldo Cruz

Secretaria da Saúde

Fundação Saúde

Universidade Federal da Bahia

Ministry of Health of the Russian Federation

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Aseptic meningitis in Belém, Brasil: first year of study [Pôster]

Maria de Lourdes Contente Gomes Lisiane Ferreira Rodrigo Henryque Gomes Z. F. G Silva C. M. F Fonseca

Aseptic meningitis

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Neutralizing Antibodies to Enterovirus 71 in Belém, Brazil

Memórias do Instituto Oswaldo Cruz (2002)

Maria de Lourdes Contente Gomes Ceyla Maria Oeiras de Castro Maria José C Oliveira Edson Elias da Silva

Non-polio enteroviruses (Coxsackievirus A, Coxsackievirus B, Echovirus and EV 68-72) which belong to the enterovirus (EV) genus, Picornaviridae family, may be responsible for acute flaccid paralysis, aseptic meningitis, myocarditis, hepatitis, pleurodinia, neonatal sepsis, hand, foot and mouth disease (HFMD) even though 50-80% of infections are asymptomatic. EV 71 has been responsible for outbreaks and epidemics of HFMD and acute neurologic disease justifying its study in our country. The aim of this study was to detect neutralizing antibodies (NtAb) to EV 71 in individuals up to 15 years of age living in Belém, State of Pará, northern Brazil. Serum samples from 238 patients attending the Virology Sector of Evandro Chagas Institute in Belém, Brazil, were analyzed using microneutralization tests that included RD cells and BrCr strain. Overall 40.8% (97/238) of tested samples had NtAb to EV 71. Regarding the distribution per age group, 85.2% (92/108) of patients aged 0-3 years had no NtAb to this virus and 69.2% of those 12 to 15 years of age were seropositive. These results confirm that EV 71 infection occurs in the city of Belém; and that a high rate of individuals in this study were infected aged 3 years and over and, when aged 15 years nearly 70% had EV 71 NtAb.

Enterovirus 71

10.1590/s0074-02762002000100006

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Detecção e caracterização de adenovírus humano proveniente de casos de paralisia flácida aguda, na Região Norte do Brasil

Revista Pan-Amazônica de Saúde (2014)

Jessylene de Almeida Ferreira Mirleide Cordeiro dos Santos Edivaldo Costa Sousa Jainara Cristina dos Santos Alves Fernando Neto Tavares

Os adenovírus humanos (HAdV) causam uma variedade de infecções, tais como as respiratórias agudas, gastrointestinais, oculares, do trato urinário e ainda síndromes neurológicas graves. O presente estudo teve como objetivo identificar e caracterizar HAdV em amostras de fezes provenientes de pacientes oriundos da Região Norte do Brasil, no período de 1998 a 2012, com quadro de paralisia flácida aguda, negativas para poliovírus e enterovírus não pólio. No estudo, 19 amostras apresentaram efeito citopático característico de HAdV quando inoculadas nas linhagens HEp2-C e L20B. Essas amostras foram submetidas à reação em cadeia mediada pela polimerase (PCR) em tempo real (qPCR) e sequenciamento nucleotídico parcial do gene que codifica a proteína héxon. Do total de amostras, 13 foram confirmadas por qPCR, PCR e sequenciamento nucleotídico. A análise genética demonstrou a seguinte caracterização viral: uma amostra como HAdV-F41; três como HAdV-A31 e nove da espécie C, sendo três HAdV-C5 e seis HAdV-C6. As amostras positivas foram provenientes dos Estados do Pará (6/19 - 31,6%), Amazonas (6/19 - 31,6%) e Acre (1/19 - 5,2%). Quanto à faixa etária, dos 13 casos positivos, 12 eram de crianças menores de 5 anos de idade, representando 92,3% dos casos. Os resultados encontrados sugerem uma possível participação dos HAdV na etiologia dos casos de paralisia flácida aguda ocorridos na Região Norte do Brasil.

10.5123/s2176-62232014000300006

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RT-PCR for confirmation of echovirus 30 isolated in Belém, Brazil

The Brazilian Journal of Infectious Diseases (2007)

Maria de Lourdes Contente Gomes Lauze Lee Alves Ferreira Rodrigo Henryque Gomes Letícia Martins Lamarão Edna da Silveira

Echovirus (Echo) 30 or human enterovirus B is the most frequent enterovirus associated with meningitis cases. Epidemics and outbreaks of this disease caused by Echo 30 have occurred in several countries. In Brazil, Echo 30 has been isolated from sporadic cases and outbreaks that occurred mainly in the south and southeast regions. We used RT-PCR to examine Echo 30 isolates from meningitis cases detected from March 2002 to December 2003 in Belém, state of Pará, in northern Brazil. The patients were attended in a Basic Health Unit (State Health Secretary of Pará), where cerebrospinal fluid (CSF) was collected and stored in liquid nitrogen. Weekly visits were made by technicians from Evandro Chagas Institute to the health unit and samples were stored at -70 degrees C in the laboratory until use. HEp-2 and RD cell lines were used for viral isolation and neutralization with specific antisera for viral identification. RNA extraction was made using Trizol reagent. The RT-PCR was made in one step, and the total mixture (50 microL) was composed of: RNA, reaction buffer, dNTP, primers, Rnase inhibitor, reverse transcriptase, Taq polymerase and water. The products were visualized in agarose gel stained with ethidium bromide, visualized under UV light. Among the 279 CSF samples examined, 30 (10.7%) were EV positive, 29 being Echo 30 and one was Cox B. Nineteen Echo 30 were examined with RT-PCR; 18 tested positive (762 and 494 base pairs). The use of this technique permitted viral identification in less time than usual, which benefits the patient and is of importance for public-health interventions.

Echovirus

Trizol

Aseptic meningitis

Ethidium bromide

10.1590/s1413-86702007000400006

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Conjuntivite hemorrágica aguda, em Cuiabá, Mato Grosso, Brasil, 1983

Revista do Instituto de Medicina Tropical de São Paulo (1987)

Elisabeth de O. Santos Olinda Macêdo Maria de Lourdes Contente Gomes Célia Maria Nakauth

Foi feita investigação de natureza etiológica clínica e laboratorial durante uma epidemia de conjuntivite hemorrágica aguda (CHA), ocorrida em Cuiabá, MT durante os meses de dezembro de 1982 e fevereiro de 1983; atendidos 68 pacientes e colhidos espécimes da conjuntiva, orofaringe e soro. Em 28 pares de soros foram realizados testes de neutralização em tubos de cultura de tecidos para o enterovirus 70 (EV 70) e fixaço do complemento para os adenovirus. Os resultados revelaram conversão sorológica de 89,3% (25 pares de soro) para o EV 70. A Secretaria de Saúde do Estado do Mato Grosso estima que aproximadamente 70% da população tenha sido atingida durante o surto. Não foram observadas evidências de comprometimento neurológico em pacientes de conjuntivite, durante ou logo após o surto.

10.1590/s0036-46651987000100008

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Conjuntivite hemorrágica aguda causada pela variante do coxsackievirus A24, em Belém, Pará, Brasil, 1987

Revista do Instituto de Medicina Tropical de São Paulo (1989)

Elisabeth de O. Santos Olinda Macêdo Maria de Lourdes Contente Gomes Célia Maria Nakauth

Durante um surto de conjuntivite hemorrágica aguda (CHA), ocorrido em Belém, Pará, Brasil, em 1987, foi feita investigação etiológica clínica e laboratorial, atendidos 83 pacientes e colhidos espécimes da conjuntiva, orofaringe e soro. Na linhagem celular HEP-2 obteve-se 73 isolamentos de um enterovírus posteriormente identificado pelo Centers for Disease Control (CDC), Atlanta, Geórgia, USA, como sendo uma variante do coxsackievirus A24. Em 56 pares de soro foram realizados testes de neutralização para o EV70 e para a cepa isolada, tendo havido 57% (32) de conversão sorológica para essa última.

Throat

10.1590/s0036-46651989000300008

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Detecção de virus corona-like em um caso de paraparesia flácida em Belém, Estado do Pará, Brasil

Revista Pan-Amazônica de Saúde (2011)

Delana Andreza Melo Bezerra José Antônio Picanço Diniz Júnior Nélson Antonio Bailão Ribeiro Maria de Lourdes Contente Gomes Alexandre da Costa Linhares

Corona (planetary geology)

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Detection and characterization of human adenoviruses from cases of acute flaccid paralysis in Northern Brazil

Revista Pan-Amazônica de Saúde (2014)

Jessylene de Almeida Ferreira Mirleide Cordeiro dos Santos Edivaldo Costa Sousa Júnior Jainara Cristina dos Santos Alves Fernando Neto Tavares

Acute flaccid paralysis

Flaccid paralysis

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Acute haemorrhagic conjunctivitis caused by coxsackievirus A24 (variant), in Belém, Pará, Brazil, 1987

Elisabeth de O. Santos Olinda Macêdo Maria de Lourdes Contente Gomes Célia Maria Nakauth

Coxsackievirus

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Echovirus 30 associated with cases of aseptic meningitis in state of Pará, Northern Brazil

Memórias do Instituto Oswaldo Cruz (2009)

Ceyla Maria Oeiras de Castro Darleise de Souza Oliveira Olinda Macêdo Maria José L Lima Marquete B. Santana

Investigation of the aetiology of viral meningitis in Brazil is most often restricted to cases that occur in the Southern and Southeastern Regions; therefore, the purpose of this study is to describe the viral meningitis cases that occurred in state of Pará, Northern Brazil, from January 2005-December 2006. The detection of enterovirus (EV) in cerebrospinal fluid was performed using cell culture techniques, RT-PCR, nested PCR and nucleotide sequencing. The ages of the 91 patients ranged from < one year old to > 60 years old (median age 15.90 years). Fever (87.1%), headache (77.0%), vomiting (61.5%) and stiffness (61.5%) were the most frequent symptoms. Of 91 samples analyzed, 18 (19.8%) were positive for EV. Twelve were detected only by RT- PCR followed by nested PCR, whereas six were found by both cell culture and RT-PCR. From the last group, five were sequenced and classified as echovirus 30 (Echo 30). Phylogenetic analyses revealed that Echo 30 detected in Northern Brazil clustered within a unique group with a bootstrap value of 100% and could constitute a new subgroup (4c) according to the phylogenetic tree described by Oberste et al. (1999). This study described the first molecular characterization of Echo 30 in Brazil and this will certainly contribute to future molecular analyses involving strains detected in other regions of Brazil.

Echovirus

Aseptic meningitis

Viral meningitis

Etiology

10.1590/s0074-02762009000300008

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Citations (12)