Adriana Brombini dos Santos

Brazilian Agricultural Research Corporation

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Francismar Corrêa Marcelino‐Guimarães

Sainsbury Laboratory

R. V. Abdelnoor

Brazilian Agricultural Research Corporation

François Belzile

Centre Hospitalier Universitaire de Sherbrooke

André Luiz de Lima Passianotto

University of Guelph

Paulo Mazzafera

Universidade Estadual de Campinas (UNICAMP)

Bruna Brumer

Universidade Estadual de Londrina

C. A. A. Arias

Brazilian Agricultural Research Corporation

Davoud Torkamaneh

Université Laval

Everton Geraldo Capote Ferreira

Sainsbury Laboratory

Rafael Soares

Universidade Estadual de Montes Claros

Cooperative Institutions

Brazilian Agricultural Research Corporation

Universidade Estadual de Campinas (UNICAMP)

Universidade Estadual de Londrina

Université Laval

Universidade de São Paulo

Universidade Federal de Viçosa

Agronomical Institute of Campinas

Ghent University

VIB-UGent Center for Plant Systems Biology

Vlaams Instituut voor Biotechnologie

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Warfarin, a potential pollutant in aquatic environment acting through Pxr signaling pathway and γ-glutamyl carboxylation of vitamin K-dependent proteins

Environmental Pollution (2014)

Ignacio Fernández Adriana Brombini dos Santos M. Leonor Cancela Vincent Laizé Paulo J. Gavaia

Pregnane X receptor

10.1016/j.envpol.2014.07.015

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Citations (42)

Water Stress Alters Lignin Content and Related Gene Expression in Two Sugarcane Genotypes

Journal of Agricultural and Food Chemistry (2015)

Adriana Brombini dos Santos Alexandra Bottcher Eduardo Kiyota Juliana Lischka Sampaio Mayer Renato Vicentini

The lignin deposition in the stem of two sugarcane genotypes was assessed on exposure to water stress. The lignin content and the morphoanatomical characterization of the stem indicated that IACSP94-2094 plants are more lignified than those of IACSP95-5000 genotype, under normal water supply conditions, which was especially associated with higher lignin contents in the rind of mature internodes. Water deficit had negative impact on the biomass production, mostly with IACSP94-2094 plants, possibly due to stress severity or higher susceptibility of that genotype during the stem-lengthening phase. Water deficit led to significant alterations in the expression levels of lignin biosynthesis genes and led to an approximate 60% increase of lignin content in the rind of young internodes in both genotypes. It is concluded that the young rind region was more directly affected by water stress and, depending on the genotype, a higher lignin accumulation may occur in the stem, thus implying lower quality biomass for bioethanol production.

Plant stem

Monolignol

10.1021/jf5061858

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Citations (42)

Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content

PLoS ONE (2015)

Renato Vicentini Alexandra Bottcher Michael dos Santos Brito Adriana Brombini dos Santos Silvana Creste

Sugarcane is an important crop worldwide for sugar and first generation ethanol production. Recently, the residue of sugarcane mills, named bagasse, has been considered a promising lignocellulosic biomass to produce the second-generation ethanol. Lignin is a major factor limiting the use of bagasse and other plant lignocellulosic materials to produce second-generation ethanol. Lignin biosynthesis pathway is a complex network and changes in the expression of genes of this pathway have in general led to diverse and undesirable impacts on plant structure and physiology. Despite its economic importance, sugarcane genome was still not sequenced. In this study a high-throughput transcriptome evaluation of two sugarcane genotypes contrasting for lignin content was carried out. We generated a set of 85,151 transcripts of sugarcane using RNA-seq and de novo assembling. More than 2,000 transcripts showed differential expression between the genotypes, including several genes involved in the lignin biosynthetic pathway. This information can give valuable knowledge on the lignin biosynthesis and its interactions with other metabolic pathways in the complex sugarcane genome.

Bagasse

Lignocellulosic Biomass

Metabolic pathway

10.1371/journal.pone.0134909

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Citations (83)

NUCLEUS IMAGE PROPERTIES AND CELL DEATH IN MCF-10F CELLS GROWN ON SLIDE SUBSTRATES DIFFERING IN NATURE AND SIZE

In Vitro Cellular & Developmental Biology - Animal (2005)

Maria Luiza S. Mello Mohamed H. Lareef Adriana Brombini dos Santos J. Russo Vidal Bc

MCF-7

10.1290/0501004.1

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Citations (3)

Lignification in Sugarcane: Biochemical Characterization, Gene Discovery, and Expression Analysis in Two Genotypes Contrasting for Lignin Content

PLANT PHYSIOLOGY (2013)

Alexandra Bottcher Igor Cesarino Adriana Brombini dos Santos Renato Vicentini Juliana Lischka Sampaio Mayer

Sugarcane (Saccharum spp.) is currently one of the most efficient crops in the production of first-generation biofuels. However, the bagasse represents an additional abundant lignocellulosic resource that has the potential to increase the ethanol production per plant. To achieve a more efficient conversion of bagasse into ethanol, a better understanding of the main factors affecting biomass recalcitrance is needed. Because several studies have shown a negative effect of lignin on saccharification yield, the characterization of lignin biosynthesis, structure, and deposition in sugarcane is an important goal. Here, we present, to our knowledge, the first systematic study of lignin deposition during sugarcane stem development, using histological, biochemical, and transcriptional data derived from two sugarcane genotypes with contrasting lignin contents. Lignin amount and composition were determined in rind (outer) and pith (inner) tissues throughout stem development. In addition, the phenolic metabolome was analyzed by ultra-high-performance liquid chromatography-mass spectrometry, which allowed the identification of 35 compounds related to the phenylpropanoid pathway and monolignol biosynthesis. Furthermore, the Sugarcane EST Database was extensively surveyed to identify lignin biosynthetic gene homologs, and the expression of all identified genes during stem development was determined by quantitative reverse transcription-polymerase chain reaction. Our data provide, to our knowledge, the first in-depth characterization of lignin biosynthesis in sugarcane and form the baseline for the rational metabolic engineering of sugarcane feedstock for bioenergy purposes.

Monolignol

Bagasse

Tricin

Lignocellulosic Biomass

Secondary cell wall

Phenylpropanoid

Saccharum

Metabolic Engineering

10.1104/pp.113.225250

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Citations (120)

Association mapping of a locus that confers southern stem canker resistance in soybean and SNP marker development

Research Square (Research Square) (2019)

João Vitor Maldonado dos Santos Everton Geraldo Capote Ferreira André Luiz de Lima Passianotto Bruna Brumer Adriana Brombini dos Santos

Abstract Background Southern stem canker (SSC), caused by Diaporthe aspalathi (E. Jansen, Castl. & Crous), is an important soybean disease that has been responsible for severe losses in the past. The main strategy for controlling this fungus involves the introgression of resistance genes. Thus far, five main loci have been associated with resistance to SSC. However, there is a lack of information about useful allelic variation at these loci. In this work, a genome-wide association study (GWAS) was performed to identify allelic variation associated with resistance against Diaporthe aspalathi and to provide molecular markers that will be useful in breeding programs. Results We characterized the response to SSC infection in a panel of 295 accessions from different regions of the world, including important Brazilian elite cultivars. Using a GBS approach, the panel was genotyped, and we identified marker loci associated with Diaporthe aspalathi resistance through GWAS. We identified 19 SNPs associated with southern stem canker resistance, all on chromosome 14. The peak SNP showed an extremely high degree of association ( p-value = 6.35E-27) and explained a large amount of the observed phenotypic variance (R² = 70%). This strongly suggests that a single major gene is responsible for resistance to D. aspalathi in most of the lines constituting this panel. In resequenced soybean materials, we identified other SNPs in the region identified through GWAS in the same LD block that clearly differentiate resistant and susceptible accessions. The peak SNP was selected and used to develop a cost-effective molecular marker assay, which was validated in a subset of the initial panel. In an accuracy test, this SNP assay demonstrated 98% selection efficiency. Conclusions Our results suggest relevance of this locus to SSC resistance in soybean cultivars and accessions from different countries, and the SNP marker assay developed in this study can be directly applied in MAS studies in breeding programs to select materials that are resistant against this pathogen and support its introgression

Canker

Genome-wide Association Study

Introgression

SNP

Genetic Association

10.21203/rs.2.11243/v2

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Citations (2)

Association mapping of a locus that confers Southern stem canker resistance in soybean

Research Square (Research Square) (2019)

João Vitor Maldonado dos Santos Everton Geraldo Capote Ferreira André Luiz de Lima Passianotto Bruna Brumer Adriana Brombini dos Santos

Abstract Background Southern stem canker (SSC), caused by Diaporthe aspalathi (E. Jansen, Castl. & Crous) is an important soybean disease, which has been responsible for severe losses in the past. The main strategy to control this fungus is through the introgression of resistance genes. So far, five main loci have been associated with resistance to Southern stem canker. However, there is a lack of information about useful allelic variation at these loci. In this work, a genome-wide association study (GWAS) was performed to identify allelic variation associated with resistance against Diaporthe aspalathi and to provide molecular markers useful in breeding programs. Results We characterized the response to Southern stem canker infection in a panel of 295 accessions from different regions of the world including important Brazilian elite cultivars. Using a GBS approach, the panel was genotyped and we identified marker loci associated with Diaporthe aspalathi resistance using GWAS analysis. We identified 19 SNPs associated with Southern stem canker resistance, all on chromosome 14. The peak SNP showed an extremely high degree of association (p-value = 6.35E-27) and explained a high level of the phenotypic variance (R2 = 70%). This strongly suggests that a single major gene is responsible for resistance to D. aspalathi present inn most of the lines comprising this panel. We also identified in resequenced soybean materials other SNPs in the region identified by GWAS in the same LD block that clearly differentiate resistance and susceptible accessions. The peak SNP was selected and used to develop a cost-effective molecular marker assay, which was validated in a subset of the initial panel. In an accuracy test, this SNP assay demonstrated 98% of selection efficiency. Conclusions Our results suggest a relevant importance of this locus in SSC resistance in soybean cultivars and accessions from different countries and the SNP marker assay developed in this study can be directly applied in MAS studies in breeding programs to select resistance materials against this pathogen and support its introgression.

Canker

Genome-wide Association Study

Introgression

SNP

10.21203/rs.2.11243/v1

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Citations (0)

Expression Profile of Sugarcane Transcription Factor Genes Involved in Lignin Biosynthesis

Tropical Plant Biology (2015)

Michael dos Santos Brito Paula Macedo Nóbile Alexandra Bottcher Adriana Brombini dos Santos Silvana Creste

Plant stem

Monolignol

Secondary cell wall

Pith

Cinnamyl-alcohol dehydrogenase

10.1007/s12042-015-9147-y

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Citations (17)

Allelic variability in the Rpp1 locus conferring resistance to Asian soybean rust revealed by genome-wide association

BMC Plant Biology (2024)

Luciano Nobuhiro Aoyagi Everton Geraldo Capote Ferreira Danielle Cristina Gregório da Silva Adriana Brombini dos Santos Bruna Barbosa Avelino

Soybean is a crucial crop for the Brazilian economy, but it faces challenges from the biotrophic fungus Phakopsora pachyrhizi, which causes Asian Soybean Rust (ASR). In this study, we aimed to identify SNPs associated with resistance within the Rpp1 locus, which is effective against Brazilian ASR populations. We employed GWAS and re-sequencing analyzes to pinpoint SNP markers capable of differentiating between soybean accessions harboring the Rpp1, Rpp1-b and other alternative alleles in the Rpp1 locus and from susceptible soybean cultivars. Seven SNP markers were found to be associated with ASR resistance through GWAS, with three of them defining haplotypes that efficiently distinguished the accessions based on their ASR resistance and source of the Rpp gene. These haplotypes were subsequently validated using a bi-parental population and a diverse set of Rpp sources, demonstrating that the GWAS markers co-segregate with ASR resistance. We then examined the presence of these haplotypes in a diverse set of soybean genomes worldwide, finding a few new potential sources of Rpp1/Rpp1-b. Further genomic sequence analysis revealed nucleotide differences within the genes present in the Rpp1 locus, including the ULP1-NBS-LRR genes, which are potential R gene candidates. These results provide valuable insights into ASR resistance in soybean, thus helping the development of resistant soybean varieties through genetic breeding programs.

Phakopsora pachyrhizi

Genome-wide Association Study

Soybean rust

10.1186/s12870-024-05454-1

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Citations (0)

Public Policies of Sports city of Jaguaquara-Bahia front of the prerogatives of the National Sports Policy.

Revista Intercontinental de Gestão Desportiva (2015)

Adriana Brombini dos Santos Thawanny Da Silva e Silva Flávio Alves Oliveira

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