Tomoyo Hamada

Tokyo Medical and Dental University

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Fumimasa Nomura

Tokyo Medical and Dental University

Kenji Yasuda

Waseda University

Tomoyuki Kaneko

The University of Tokyo

Akihiro Hattori

Waseda University

Hideyuki Terazono

Kagoshima University

Yasuyuki Abe

Prefectural University of Hiroshima

Atsushi Sanbuissho

Daiichi-Sankyo (Japan)

Noriaki Ikemoto

Denso (United States)

Kiyoshi Takasuna

Daiichi-Sankyo (Japan)

Hiroaki Kagawa

TEPCO (Japan)

Cooperative Institutions

Tokyo Medical and Dental University

Kanagawa Academy of Science and Technology

Gwangju Institute of Science and Technology

Daiichi-Sankyo (Japan)

Waseda Bioscience Research Institute in Singapore

Harvard University

Kootenay Association for Science & Technology

Boston Biomedical Research Institute

The University of Tokyo

Seoul National University

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2J1436 オンチップ多電極計測システムを用いたヒトES細胞由来心筋細胞塊の細胞外電位計測(計測、その他,第49回日本生物物理学会年会)

生物物理 (2011)

Tomoyuki Kaneko Fumimasa Nomura Tomoyo Hamada Kenji Yasuda

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The Ryanodine Receptor Pore Blocker Neomycin also Inhibits Channel Activity via a Previously Undescribed High-Affinity Ca2+ Binding Site

The Journal of Membrane Biology (2007)

Derek R. Laver Tomoyo Hamada James D. Fessenden Noriaki Ikemoto

Neomycin

10.1007/s00232-007-9067-3

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Citations (10)

3P216 A comparative study on electrophysiological properties of human iPS- and ES-derived cardiomyocytes(13C. Biological & Artifical membrane: Excitation & Channels,Poster)

Seibutsu Butsuri (2013)

Fernando Martínez López Junko Kurokawa Fumimasa Nomura Tomoyuki Kaneko Tomoyo Hamada

Cell membrane and organelle have many kinds of lipids.The distribution is changed depending on the function.To study the lipid dynamics, the behavior of lipid molecules is important.There are two lipid movements, lateral diffusion within a leaflet and flip-flop between leaflets.In contrast to the fast lateral diffusion, flip-flop is rather slow because of the energy barrier to leap the polar head group over the hydrophobic core in membrane.Ceramide, cell-signaling messenger lipid, has small head and this might promotes flip-flop.Therefore, we synthesized sulfhydryl ceramide analogues to quantify the ceramide flip-flop in model membrane.This system clarifies the ceramide flip-flop is affected by lipids composition, such as fluidity and lipid backbone structure.

10.2142/biophys.53.s247_5

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2P287 Measurement of contractile direction on single-shapecontrolled cardiomyocytes by on-chip optical image analysis system(26. Measurements,Poster)

Seibutsu Butsuri (2013)

Tomoyuki Kaneko Fumimasa Nomura Tomoyo Hamada Akihiro Hattori Kenji Yasuda

Recently, artificial cells have been attracted much attention as a model of living cells.The living cells are non-equilibrium chemically open systems and transport molecules in and out through their cell membrane in a controlled manner.However, artificial cells with a chemically open feature and a controlled transport of molecules have never been achieved yet.Here, we propose a chemically open artificial cell with a feedback control over molecular permeability of the cell membrane.To implement this feedback control system, diffusion coefficient inside the artificial cell is changed according to the concentration of inside chemicals.We believe that this numerical analysis will promote the understanding of dynamic chemical systems in artificial cells.

10.2142/biophys.53.s206_4

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2J1424 Development of the evaluation system for drug-induced cardiac toxicity by using on-chip cell multi electrode assay measurements(Measurements, Miscellaneous topics,The 48th Annual Meeting of the Biophysical Society of Japan)

Seibutsu Butsuri (2011)

Tomoyo Hamada Tomoyuki Kaneko Fumimasa Nomura Kenji Yasuda

Drug toxicity

Drug Development

10.2142/biophys.51.s91_2

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Quasi-In Vivo Electrocardiogram Measurement using Convolution of Field Potential Propagation in the On-Chip Cardiomyocytes Network Circuit

Biophysical Journal (2012)

Fumimasa Nomura Tomoyuki Kaneko Tomoyo Hamada Akihiro Hattori Kenji Yasuda

Multielectrode array

10.1016/j.bpj.2011.11.2971

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Toward quasi-in vivo from in vitro assay (II): Development of on-chip predictive cardiotoxicity assay for cardiac contraction fluctuation measurement using dual recording of electrical field potential and optical image analysis

Journal of Pharmacological and Toxicological Methods (2013)

Tomoyuki Kaneko Fumimasa Nomura Tomoyo Hamada Akihiro Hattori Kenji Yasuda

Cardiotoxicity

10.1016/j.vascn.2013.01.095

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3PS020 心毒性検査での細胞外電位記録における心筋細胞拍動周期制御のための電気刺激方法の最適化(日本生物物理学会第50回年会(2012年度))

生物物理 (2012)

Tomoyuki Kaneko Fumimasa Nomura Tomoyo Hamada Kenji Yasuda

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Importance of Thickness in Human Cardiomyocyte Network for Effective Electrophysiological Stimulation Using On-Chip Extracellular Microelectrodes

Japanese Journal of Applied Physics (2012)

Tomoyo Hamada Fumimasa Nomura Tomoyuki Kaneko Kenji Yasuda

We have developed a three-dimensionally controlled in vitro human cardiomyocyte network assay for the measurements of drug-induced conductivity changes and the appearance of fatal arrhythmia such as ventricular tachycardia/fibrillation for more precise in vitro predictive cardiotoxicity. To construct an artificial conductance propagation model of a human cardiomyocyte network, first, we examined the cell concentration dependence of the cell network heights and found the existence of a height limit of cell networks, which was double-layer height, whereas the cardiomyocytes were effectively and homogeneously cultivated within the microchamber maintaining their spatial distribution constant and their electrophysiological conductance and propagation were successfully recorded using a microelectrode array set on the bottom of the microchamber. The pacing ability of a cardiomyocyte's electrophysiological response has been evaluated using microelectrode extracellular stimulation, and the stimulation for pacing also successfully regulated the beating frequencies of two-layered cardiomyocyte networks, whereas monolayered cardiomyocyte networks were hardly stimulated by the external electrodes using the two-layered cardiomyocyte stimulation condition. The stability of the lined-up shape of human cardiomyocytes within the rectangularly arranged agarose microchambers was limited for a two-layered cardiomyocyte network because their stronger force generation shrunk those cells after peeling off the substrate. The results indicate the importance of fabrication technology of thickness control of cellular networks for effective extracellular stimulation and the potential concerning thick cardiomyocyte networks for long-term cultivation.

Multielectrode array

10.7567/jjap.51.06fk03

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Citations (1)

Toward quasi-in vivo from in vitro assay (I): On-chip cardiomyocyte network screening assay for predictive cardiotoxicity

Journal of Pharmacological and Toxicological Methods (2013)

Kenji Yasuda Fumimasa Nomura Tomoyo Hamada Tomoyuki Kaneko Yasuyuki Abe

Cardiotoxicity

10.1016/j.vascn.2013.01.094

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