Mingyang He

South China Botanical Garden

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Yueming Jiang

South China Botanical Garden

Yuejin Wang

Northwest A&F University

Min Hong

Citrus Research Institute

Taotao Li

South China Botanical Garden

Wang Rikui

Ziguo Zhu

Shandong Academy of Agricultural Sciences

Jiangling Cao

Nanchang University

Xuewu Duan

South China Botanical Garden

Jiangli Shi

Henan Agricultural University

Yazhou Yang

Northwest A&F University

Cooperative Institutions

Chinese Academy of Sciences

Northwest A&F University

South China Botanical Garden

Ministry of Agriculture and Rural Affairs

University of Chinese Academy of Sciences

Southwest University

China National Botanical Garden

Shanghai Jiao Tong University

Changzhou University

Beijing Botanical Garden

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Confirmation of ‘Pollen- and Seed-Specific Gene Deletor’ System Efficiency for Transgene Excision from Transgenic Nicotiana tabacum under Field Conditions

International Journal of Molecular Sciences (2023)

Zhenzhen Duan Mingyang He Sehrish Akbar Degang Zhao Muqing Zhang

The commercial application of genetically modified plants has been seriously impeded by public concern surrounding the potential risks posed by such plants to the ecosystem and human health. Previously, we have developed a ‘pollen- and seed-specific Gene Deletor’ system that automatically excised all transgenes from the pollen and seeds of greenhouse-grown transgenic Nicotiana tabacum. In this study, we conducted seven field experiments over three consecutive years to evaluate the stability of transgene excision under field conditions. Our results showed that transgenes were stably excised from transgenic Nicotiana tabacum under field conditions with 100% efficiency. The stability of transgene excision was confirmed based on PCR, as well as the GUS staining patterns of various organs (roots, leaves, petiole, stem, flower, fruit, and seeds) from transgenic N. tabacum. In six transgenic lines (D4, D10, D31, D56, and D43), the transgenes were stably deleted in the T0 and T1 generations. Thus, the ‘Gene Deletor’ system is an efficient and reliable method to reduce pollen- and seed-mediated unintentional gene flow. This system might help to alleviate the food safety concerns associated with transgenic crops.

10.3390/ijms24021160

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CCDC 981975: Experimental Crystal Structure Determination

The Cambridge Structural Database (2014)

Sheng‐Chun Chen Xinxin Wang Hai‐Kun Luo Mingyang He Cheng‐Peng Li

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Crystal (programming language)

10.5517/cc11ytmf

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Choline chloride alleviates the pericarp browning of harvested litchi fruit by inhibiting energy deficiency mediated programmed cell death

Postharvest Biology and Technology (2020)

Taotao Li Mingyang He Jun Zeng Zhongsuzhi Chen Qu Hongxia

Browning

Betaine

Choline chloride

Energy charge

Choline

10.1016/j.postharvbio.2020.111224

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Citations (19)

Three ERF transcription factors from Chinese wild grapevine Vitis pseudoreticulata participate in different biotic and abiotic stress-responsive pathways

Journal of Plant Physiology (2013)

Ziguo Zhu Jiangli Shi Weirong Xu Huie Li Mingyang He

Methyl jasmonate

Biotic stress

Jasmonic acid

Ralstonia solanacearum

Defence mechanisms

Jasmonate

Pseudomonas syringae

10.1016/j.jplph.2013.01.017

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Citations (61)

Study on nine prophage genetic markers in multidrug-resistant Escherichia coli

Zhongguo yiyuan ganranxue zazhi (2012)

Wang Yi-ping Guozhong Chen Lu Yong Jianfei Ying Yu YanHong

OBJECTIVE To investigate the distribution of nine prophage genetic markers in multidrug-resistant Escherichia coli.METHODS PCR was used to detect the nine prophage genetic markers(intF,intD,intE,intR,intQ,flu,intS,intZ,and intA) in E.coli and two cell division genes(dicB,kilR),and these genetic markers were carried out for database retrieval in BIOCYC.RESULTS Totally five prophage genetic markers including intD,intE,intR,intQ,and intS,and two cell division genes(dicB,kilR) were detected in 20 strains of multidrug-resistant E.coli;intF,flu,intZ,or intA4 were not detected,there wer 14 strains detected with more than 1 gene related to the cell division;as compared with the sequence of positive intR in No.2 strain determined by BLAST,it was as same as the sequence of CP002516 having registered in GenBank(by Mar 9,2011);as compared with the sequence of positive intQ in No.8 strain determined by BLAST,it was as 99%same as the sequence of AP010960.1 having registered in GenBank.CONCLUSION It is necessary to have a further study on the mechanism and function of prophage genetic marker,which may play as a supportive factor in inducing high multidrug-resistance of multidrug-resistant E.coli.

Prophage

Strain (injury)

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Mycoplasma Infection in Intensive Care Unit

Zhongguo yiyuan ganranxue zazhi (2010)

Guozhong Chen Yiping Wang An Minfei Zhou Cheng-jie Tang BoQin

OBJECTIVE To investigate mycoplasma infection in ICU patients.METHODS Sixty-five samples from blood,respiratory tract and genitourinary tract of patients were collected respectively from Oct 2007 to July 2008 in ICU.Mycoplasma pneumoniae(Mp),Urealasma urealytium(Uu) M.fermentans(Mf) and M.penetrans(Mpe) were cultivated by modified mycoplasma fluid and solid medium.Mf and Mpe positive isolates were verified by nested polymerase chain reaction(rPCR),Mp and Uu were confirmed by fluorescent quantitative PCR.RESULTS It was found that the positive detection rate for Mp was 12.3%(8/65)in blood and 35.4%(23/65) in respiratory tract excreta and for Mu 1.5%(1/65) and 26.2%(17/65) in blood or Genitourinary tract,respectively.Mpe and Mf did not detected.CONCLUSIONS The state of mycoplasma infection is very severe,and often accompanies bacterial infection.It is necessary to consider mycoplasma when chose antibiotics.

Respiratory tract

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Drug-resistant Genes Associated with Aminoglycosides in Acinetobacter baumannii Isolated from ICU

Zhongguo yiyuan ganranxue zazhi (2009)

Mingyang He

OBJECTIVE To investigate the antibiotic resistance of multi-resistant Acinetobacter baumannii(ABA) and distribution of aminoglycoside-modifying enzymes and 16S rRNA methylase genes in ICU in Yinzhou People′s Hospital in Ningbo. METHODS The samples of 20 ABA isolates were collected from Oct 2007 to Jul 2008 in ICU.K-B method was used to determine the sensitivity to 32 antibacterials and the aminoglycoside-modifying enzymes and 16S rRNA methylase genes were analyzed by polymerase chain reaction(PCR). RESULTS From 20 ABA isolates,8 strains carried aminoglycoside-modifying enzymes genes aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb,and ant(3″)-Ⅰ,their positive rate was 10%,15%,30% and 25%,respectively;no strain carried 16S rRNA methylase genes. CONCLUSIONS The antibiotics resistance of A.baumannii is very serious in Yinzhou People′s Hospital in Ningbo.Aminoglycoside-modifying enzymes genes aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb and ant(3″)-Ⅰ exist in multi-resistant A.baumannii widely.They would be the main causes of high drug-resistantce to aminoglycosides.

Acinetobacter baumannii

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収穫後の柑橘類と果皮中の果皮成分の動的変化を観察した。【JST・京大機械翻訳】

Shipin Kexue (2016)

Ge Zhixing Mingyang He Zeng Xiao-feng Huang Xiaolan Wang Rikui

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CCDC 672825: Experimental Crystal Structure Determination

The Cambridge Structural Database (2008)

Shengchun Chen Qun Chen Qi Liu Chao Li Mingyang He

10.5517/ccql41g

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Expression of a Chromoplast-Specific Lycopene β-Cyclase Gene (CYC-B) Is Implicated in Carotenoid Accumulation and Coloration in the Loquat

Biomolecules (2019)

Min Hong Zhuoheng Chi Yongqing Wang Yueming Tang Qunxian Deng

Carotenoids are the principal pigments in the loquat. Although the metabolic pathway of plant carotenoids has been extensively investigated, few studies have been explored the regulatory mechanisms of loquat carotenoids because knowledge of the loquat genome is incomplete. The chromoplast-specific lycopene β-cyclase gene (CYC-B) could catalyze cyclization of lycopene to β-carotene. In this study, the differential accumulation patterns of loquat with different colors were analyzed and virus-induced gene silencing (VIGS) was utilized in order to verify CYC-B gene function. Using a cloning strategy of homologous genes, a CYC-B gene orthologue was successfully identified from the loquat. At a later stage of maturation, CYC-B gene expression and carotenoids concentrations in the ‘Dawuxing’ variety were higher than in ‘Chuannong 1-5-9′, possibly leading to the difference in pulp coloration of loquat. Interference of CYC-B gene expression in the loquat demonstrated clear visual changes. The green color in negative control fruits became yellow, while TRV2-CYC-B silenced fruits remained green. CYC-B gene expression and total carotenoid content in the pulp decreased by 32.5% and 44.1%, respectively. Furthermore, multiple key genes in the carotenoid metabolic pathway synergistically responded to downregulation of CYC-B gene expression. In summary, we provide direct evidences that CYC-B gene is involved in carotenoid accumulation and coloration in the loquat.

Chromoplast

10.3390/biom9120874

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Citations (13)