Solitary extramedullary plasmacytomas are plasma cell tumors that tend to develop in mucosa-associated lymphoid tissues including the sinonasal or nasopharyngeal regions. Primary plasmacytoma of the lung is exceedingly rare and often presents as a solitary mass or nodule in mid-lung or hilar areas and diagnosed after resection. Herein, we report a case of primary pulmonary plasmacytoma that presented with diffuse alveolar consolidation and diagnosed by transbronchial lung biopsy.
Identification of gene rearrangements and clonality analysis are important techniques for the diagnosis of malignant lymphoproliferative diseases. These methods have various sensitivities based on the type of primer used and method of determination of polymerase chain reaction (PCR) products. This study aimed at determining the clonality of B cell non-Hodgkin lymphoma in Iranian patients using PCR method and 2 primers of FR2 and FR3.Paraffin embedded blocks of 67 patients with B cell lymphoma and 19 cases with lymphoid hyperplasia of the lymph nodes who presented to NRITLD, Masih Daneshvari Hospital were retrospectively reviewed. After extracting the genomic DNA using phenol and chloroform, clonal analysis was performed using semi-nested PCR by using two primers: FR2 and FR3. PCR products were determined using 2 techniques of heteroduplex analysis, polyacrylamide gel and silver staining and the conventional method of agarose gel and ethidium bromide staining. Appearance of 1 or 2 bands in the desired location were considered as a sign of clonality.Monoclonal gene rearrangement was observed in 62 out of 67 patients (92.5%) as one or two discrete bands appeared within 60-120 base pairs (bp) and 200-300 bp range. Of the mentioned patients, 53 cases (79.1%) had FR2 and 51 (76.1%) had FR3 rearrangement. Heteroduplex analysis along with silver nitrate staining detected 3 out of the remaining 5 cases of lymphoma to be monoclonal. These cases had been reported negative by the conventional technique. In total, 65 out of 67 patients (97%) showed monoclonal gene rearrangement using both the abovementioned techniques. All hyperplasia cases were polyclonal by this method.Our study showed that evaluation and detection of clonality using PCR, FR2 and FR3 primers along with heteroduplex analysis is a rapid sensitive technique for the diagnosis of malignant lymphomas.
Systemic lymph node metastasis is a rare event in malignant mesothelioma. It is even more exceptional when systemic lymph node metastasis is the initial clinical presentation. Review of literature discloses only four cases in which metastatic lymphadenopathy was the only symptom of malignant mesothelioma. We, herewith, report three cases where the initial diagnosis of malignant mesothelioma was made by biopsy of enlarged lymph nodes, which were the only clinical presentation. Immunohistochemistry played a pivotal role in elucidating the mesothelial origin of their unusual systemic lymph node metastasis.
Background and Aim: Squamous cell carcinoma (SCC) constitutes 94% of oral malignancies. In recent decade many molecular and genetic changes have been known to affect tumoral behavior which might be good targets for new drug production. It is known that expression of EGF is correlated with poor prognosis and expression of laminin–5 can lead to the development of premalignant lesions to in situ or invasive malignancies. The aim of this study was to determine the correlation between the expression of EGFR and laminin–5 with clinical stage and microscopic grade of oral SCC. Materials & Methods: Forty two paraffin blocks with diagnosis of SCC were collected from oral pathology department of Shahid Beheshti dental school. Immunohistochemical staining was performed with antibodies against EGFR and laminin –5. Two pathologists counted the number of stained cells under light microscope and expression of these two markers was compared by Kendal test. Results: No significant correlation was found between expression of EGFR and tumoral stage and grade. But a significant correlation was found between the expression of laminin-5 with tumoral grade and stage was found (p less than 0.01). Conclusion: It is concluded that laminin–5 is a marker with a significant effect in developing of a higher tumoral stage and grade but more precise researches are needed to identifying the role of EGFR.
Abstract Nod-like receptor pyrin domain-containing-3 (NLRP3) has been implicated in the pathogenesis of experimental renal injury, yet its characterization in human kidney disease remains largely unexplored. NLRP3 expression was evaluated in human kidney biopsies, primary renal tubular cells (HPTC) and correlated to disease outcomes in patients with IgA nephropathy (IgAN). NLRP3 localized to renal tubules in normal human kidney tissue and to mitochondria within HPTC by immunohistochemistry and immunofluorescence microscopy. Compared to control kidneys, NLRP3 gene expression was increased in biopsies of patients with IgAN. While NLRP3 expression in IgAN was detected in glomeruli, it remained largely confined to the tubular epithelial compartment. In vitro NLRP3 mRNA and protein expression were transiently induced in HPTC by TGF-β1 but subsequently diminished over time as cells lost their epithelial phenotype in a process regulated by transcription and ubiquitin-mediated degradation. Consistent with the in vitro data, low NLRP3 mRNA expression in kidney biopsies was associated with a linear trend of higher risk of composite endpoint of doubling serum creatinine and end stage renal disease in patients with IgAN. Taken together, these data show that NLRP3 is primarily a kidney tubule-expressed protein that decreases in abundance in progressive IgAN.
This paper was published in BioImpacts in 2013 (doi: 10.5681/bi.2013.005).1 Based on an email received from a researcher recently, we were informed that this published paper presented some data (i.e., Fig. 3a and 3b) as same as data presented in three other published papers (i.e., doi: 10.1111/Cbdd.12318; doi: 10.1111/Cbdd.12318; and doi: 10.1186/1477-3155-10-46),2-4 which are considered as the ethical misconduct. This issue was brought up with and comprehensively investigated by the editorial team of BioImpacts through comparison of the papers and the within-data. As a result, in accordance with the Committee on Publication Ethics (COPE), the editors decided to retract this paper. The authors were informed and advised on this serious ethical breach. Based on the COPE guidelines on the retraction, BioImpacts considers retracting a publication if: - It has clear evidence that the findings are unreliable, either as a result of misconduct (e.g., data fabrication and/or falsification) or honest error (e.g. miscalculation or experimental error). - The findings have previously been published elsewhere without proper crossreferencing, permission or justification (i.e. cases of redundant publication). As a peer-review multidisciplinary international "Publish Free" and "Access Free" journal, BioImpacts strongly adheres to the "Publication Ethics", and its foremost goal is to preserve the integrity of the scientific reports in the highest standards, therefore, the journal takes all issues of publication misconduct seriously.
Background and Objectives: Transbronchial lung biopsy (TBLB) is the method of choice for diagnosis of pulmonary parenchymal lesions and is used for a wide array of pulmonary diseases. It is also a safe applicable method in ambulatory patients. This study sought to assess the factors affecting the diagnostic value of TBLB. Materials and Methods : This prospective, observational double blind study was conducted on 44 patients presenting to the Bronchoscopy Ward of Masih Daneshvari Hospital in Tehran. Four TBLB specimens were obtained from each patient and characteristics of specimens (size, number of alveoli, diagnostic value and floatability) were recorded. Results: A total of 176 samples were obtained from 44 patients including 21 males (47.7%) and 23 females (52.3%). In general, 37 samples (21%) were diagnostic. Of 57 small samples, 12 (21.2%), of 66 medium size samples, 12 (18.2%), and of 53 large size samples, 13 (24.5%) were diagnostic (P=0.700). No significant association was found between the size of specimen and its diagnostic value. Of 84 alveolated samples 31 (36.9%), of 26 non-alveolated samples 5 (19.2%) and of 66 samples with no alveoli one (1.5%) had a diagnostic value (P Conclusion: Based on the obtained results, number of alveoli present in each specimen was correlated to its diagnostic value. Size or floatability of specimens had no effect on their quality. Increasing the number of specimens to four can increase the likelihood of reaching a definite diagnosis.
Background: Idiopathic pulmonary fibrosis (IPF) is characterized by a chronic inflammatory process and abnormal wound healing. Tumor Necrosis Factor alpha (TNF �) is considered to play a key role in fibroblast proliferation and increased collagen synthesis. It appears that there is a genetic predisposition to IPF. The genetic associations of TNF-� with IPF have been reported in different cohorts and revealing conflicting results. This study was conducted to evaluate the association of TNF- alpha-308 G/A polymorphism with IPF in Iranian patient by PCR-RFLP method.
Background Obstructive sleep apnea (OSA) is one of the most common sleep disorders with a plethora of consequences. Currently the impact of hypoxia due to OSA on liver function is targeted growing attention. This study evaluated the association between serum aminotransferase levels as accepted predictive factor for liver injury and factors connected with OSA severity (apnea hypopnea index (AHI), lowest oxygen saturation level, oxygen desaturation index (DI), percent of time below 90% saturation [T Materials and Methods 66 patients with BMI≥30, who their OSA was confirmed by PSG, were divided equally into two groups based on AHI. 33 patients in control group with 5 Result Mean DI was (56.33 ± 26.97) (14.00 ± 10.46) in case and control group respectively (P-value Conclusions This study showed there is no significant correlation between serum aminotransferase levels and OSA severity.
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