A 44-year-old male complained recurrent swelling and pain in the left pre-auricular mass over 4 years, which aggravated for 4 months. Ultrasonography revealed a mixed mass echo in the left parotid gland. Computed tomography showed an enlarged parotid gland and a 1.5 cm x 0.9 cm low density shadow in the superficial lobe area with strip high-density shadow in the edge. Although the facial nerves of the patient were found adhered to the foreign body during the operation, the foreign body in parotid gland was removed surgically without any injure to them due to facial nerves protection. In conclusion, facial nerves dissection is necessary to avoid the postsurgical facial paralysis in the surgical removal of foreign bodies in parotid gland.
Abstract This study sought to investigate the volatile flavor profile of foreleg meat from different lamb breeds and to compare the flavor differences among Qianbei Brown Goat (QBG), Tan Sheep (TS), and Sunit Sheep (SS) using headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC–MS) and a relative odor activity value (rOAV) > 1. Principal component analysis (PCA) and orthogonal partial least squares discrimination analysis (OPLS-DA) were used to analyse metabolic changes. Differential metabolites were selected based on a VIP > 1, P < 0.05, fold change > 2, or < 0.5. ROAV > 1 combined with flavouromics was used for aroma analysis. Flavoromics analysis showed notable differences in aroma profiles among the TS, QBG, and SS groups. The TS group exhibited the strongest green, fruity, fresh, and fatty flavors. Seven key aroma compounds were identified as contributors to the aroma variations among different lamb breeds. These findings reveal flavor variations between lamb breeds.
Abstract Background: Circular RNAs (circRNAs) have been reported to play key roles in the development of various cancers. However, the biological functions and clinical significance of most circRNAs are still elusive. The purpose of this study was to explore the function and mechanism of a certain circRNA named circCDKN2B-AS1 in cervical cancer development and its potential value in the clinic. Methods: qRT-PCR was used to verify the expression level of circCDKN2B-AS1. CCK-8, Transwell, and flow cytometry (FCM) assays were performed to detect cellular proliferation, migration, and apoptosis, respectively. A Seahorse XFe96 Analyzer was used to measure glycolysis metabolism level. RNA pull-down, RNA immunoprecipitation (RIP), actinomycin-D addition assays and Western blotting were used to screen and elucidate the potential mechanisms involved. BALB/c nude mice and zebrafish embryos (AB, WT) were used as animal models to investigate tumorigenesis capability. 18 FDG-microPET/CT imaging and lactic acid (LA) and pyruvic acid (PA) content detection assays were used to detect the level of glucose metabolism in subcutaneous tumors from nude mice. Results: CircCDKN2B-AS1, a circular isoform of the long noncoding RNA (lncRNA) CDKN2B-AS1, was upregulated in cervical cancer and precancerous tissues. We found that circCDKN2B-AS1 associated with the IMP3 protein depending on a specific binding site and regulated the stability of Hexokinase 2 (HK2) mRNA, the rate-limiting enzyme of the aerobic glycolysis pathway. The expression level of circCDKN2B-AS1 fated the binding of IMP3 to the 3’ untranslated region (UTR) of HK2 mRNA, consequently affecting the malignant cell phenotype and aerobic glycolysis in cervical cancer in vitro and in vivo. Mutant circCDKN2B-AS1, lacking the IMP3 binding site, did not have such effects. Utilization of an inhibitory peptide to block the interaction between circCDKN2B-AS1 and the IMP3 protein impeded the binding of IMP3 to the 3’UTR of HK2 mRNA and suppressed aerobic glycolysis in cervical cancer cells. Conclusions: Our findings demonstrate that circCDKN2B-AS1 facilitates aerobic glycolysis by sponging the IMP3 protein to stabilize HK2 mRNA, consequently promoting the malignant phenotype in cervical cancer, which may provide a potential approach for cervical cancer therapeutics.
Objective: To compare the safety between conization alone and hysterectomy for patients with adenocarcinoma in situ (AIS) of the cervix. Design: A cohort study of AIS patients during 2007-2021. Setting: Women’s Hospital of Zhejiang University School of Medicine. Population: A total of 453 AIS patients diagnosed by conization who underwent cervical conizations only (n=153) or conization followed by hysterectomy(n=300). Methods: The clinicopathological and follow-up data were reviewed. Univariate analysis was examined by chi-square test and multivariate analysis was performed by logistic regression analysis. Main outcome measure: The rates of residual disease in specimens of hysterectomy and repeated conization were compared between positive and negative margin of conization. And the rates of recurrence were compared between patients treated by conization(s) alone and hysterectomy. The factors influencing residual disease and recurrence were assessed, respectively. Results: Among 310 specimens of hysterectomy or repeated conization, the rates of residual disease were 50.56% (45/89) for positive margin and 2.26% (5/221) for negative margin, with a significant difference (p=0.000). Totally 4 patients recurred as vaginal intraepithelial neoplasia（VAIN）in those treated by hysterectomy and 1 recurred as cervical squamous intraepithelial neoplasia (CIN) in those treated by conization(s) alone. The rates of recurrence were 0.65% (1/153) for conization(s) alone and 1.33 % (4/300) for hysterectomy, with no significant difference (p=0.431). Hysterectomy was a factor influencing neither residual disease nor recurrence. Conclusions: Conization is an effective and safe option for patients with AIS of the cervix, provided the margin is negative. Keywords: adenocarcinoma in situ, conization, hysterectomy, margins.
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