Wenbo Xu

Third Affiliated Hospital of Southern Medical University

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Yan Zhang

Capital Medical University

Naiying Mao

National Institute for Viral Disease Control and Prevention

Zhen Zhu

National Institute for Viral Disease Control and Prevention

Guizhen Wu

National Institute for Viral Disease Control and Prevention

Ai‐Li Cui

Ministry of Agriculture and Rural Affairs

Xiang Zhao

National Institute for Viral Disease Control and Prevention

George F. Gao

Institute of Microbiology

Wenjie Tan

Chinese Center For Disease Control and Prevention

Lin Xu

National Institute for Viral Disease Control and Prevention

Hu Ren

National Institute for Viral Disease Control and Prevention

Cooperative Institutions

National Institute for Viral Disease Control and Prevention

179

Chinese Center For Disease Control and Prevention

156

Chinese Academy of Medical Sciences & Peking Union Medical College

153

Chinese Academy of Sciences

116

Capital Medical University

101

Center for Disease Control

Shanghai Jiao Tong University

University of Chinese Academy of Sciences

Zhejiang University

Peking University

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Identification of miRNA signature in cancer-associated fibroblast to predict recurrent prostate cancer

Computers in Biology and Medicine (2024)

Wenbo Xu Shuai Liu Longtu Ma Long Cheng Qingchao Li

Identification

Signature (topology)

10.1016/j.compbiomed.2024.108989

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Citations (1)

Gene conversion challenges in preimplantation genetic testing for monogenic disease (PGT-M)

Molecular Genetics and Metabolism (2021)

Yan Bai Youbao Sha Wenbo Xu J. Dianne Keen-Kim

10.1016/s1096-7192(21)00503-5

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A multiomic ferroptosis-associated prognostic signature incorporating epigenetic and transcriptional biomarkers for hepatocellular carcinoma

Translational Cancer Research (2022)

Jida Chen Xinli Zhu Danzhi Chen Lidan Jin Wenbo Xu

Ferroptosis was reported to have tremendous promise in the treatment and prognosis of hepatocellular carcinoma (HCC). Here, we identified a novel ferroptosis-related prognostic signature incorporating epigenetic and transcriptional biomarkers could help predicting survival of patients with HCC.We employed multi-omics and clinical data from The Cancer Genome Atlas (TCGA) database to identify the ferroptosis-associated methylation CpG sites associated with HCC survival using sure independence screening (SIS). Then we utilized Kaplan-Meier curves to evaluate the prognostic significance of gene expression and DNA methylation. Receiver operating characteristic (ROC) curve was used predicting the 3- and 5-year survival. Mediation analysis of ferroptosis-related methylation and transcriptional score was performed.We firstly identified 114 significant CpG sites under the criteria of false discovery rate (FDR) <0.05 in training set. Then we screened out 5 candidate CpG sites in validation set for multivariate screening and stepwise regression. We found that the high-risk group had significantly shorter survival time than the low-risk group in the prognostic signature combined with epigenetic and transcriptional scores (HR =2.72 95% CI: 2.01-3.68, P=8.75E-11). And the predictive model involving clinical information, gene expression, and methylation data performed best for 3-year survival prediction (AUC =0.672) and 5-year survival prediction (AUC =0.742).Our results suggested a signature combining clinical information, ferroptosis-related gene expression, and methylation presented a superior ability for prognostic prediction in HCC, which may bring us novel tool and targets in the treatment of HCC.

CpG site

Gene signature

10.21037/tcr-21-2882

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Correction: Viral Etiology of Acute Respiratory Infection in Gansu Province, China, 2011

PLoS ONE (2013)

Guohong Huang Deshan Yu Naiying Mao Zhen Zhu Hui Zhang

Background: Acute respiratory infections (ARIs) are the leading cause of children and their leading killer.ARIs are responsible for at least six percent of the world's disability and death.Viruses are one of the most common agents causing ARIs.Few studies on the viral etiology and clinical characteristics of ARIs have been performed in the northwest region of China, including Gansu Province.Methods: Clinical and demographic information and throat swabs were collected from 279 patients from January 1st to December 30st, 2011.Multiplex RT-PCR was performed to detect 16 respiratory viral pathogens.Results: 279 patients were admitted for ARIs.The patients aged from 1 month to 12 years, with the median age of 2 years.Of which, 105 (37.6%) were positive for at least one pathogen.A total of 136 respiratory viral pathogens were identified from the 105 patients.Respiratory syncytial virus (RSV) was the most frequently detected pathogen (26.5%, 36/136), followed by parainfluenza virus (PIV) 1-3 (22.1%, 30/136), human rhinovirus (HRV) (21.3%, 29/136), human coronavirus (CoV) (10.3%, 14/136) and human adenovirus (HAdV) (9.6%, 13/136).Influenza A (Flu A), human metapneumovirus (hMPV) and human bocavirus (BoCA) were found 4.4%, 3.7% and 2.2%, respectively.Influenza B (Flu B) and seasonal influenza A H1N1(sH1N1) were not detected.Single-infections were detected in 30.5% (85/279) of cases.RSV was the most common pathogens in patients under 1 year and showed seasonal variation with peaks during winter and spring.Conclusions: This paper presents data on the epidemiology of viral pathogens associated with ARIs among children in Gansu Province, China.RSV is most frequently detected in our study.The findings could serve as a reference for local CDC in drawing up further plans to prevent and control ARIs.

10.1371/annotation/91d141f8-549c-475c-891a-5d8b4e5f91fd

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Emergence of BA9 genotype of human respiratory syncytial virus subgroup B in China from 2006 to 2014

Scientific Reports (2017)

Jinhua Song Huiling Wang Jing Shi Ai‐Li Cui Yanzhi Huang

A study was conducted to investigate the circulation of HRSV subgroup B (HRSVB) in China in recent years. HRSVB sequences from 365 samples collected in 1991, 2004 and 2008-2014 in China, together with 332 Chinese HRSVB sequences obtained from GenBank were analyzed to determine the geographic and yearly distribution of HRSVB. Phylogenetic analysis revealed these HRSVB sequences clustered into 4 genotypes with different frequencies: BA (83%), CB1 (11%), SAB (3.0%) and GB3 (0.7%). Between 2005 and 2013, there was a co-circulation of BA and non-BA genotypes in China. Genotypes BA9 and BA10 were two of the main BA genotypes detected in this study. Genotype BA9 was first detected in China in 2006 and became the predominant HRSVB genotype circulating in China from 2008 to 2014. Three different lineages were detected for both genotypes BA9 and BA10. Time to the most recent common ancestor for genotypes BA9 and BA10 was estimated for years 1997 and 1996, respectively. Results of this study not only contribute to the understanding of the circulation pattern, but also the phylogenetic pattern and evolution of HRSVB in China from 1991 to 2014.

10.1038/s41598-017-17055-0

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[Genotype and genetic characteristics of human respiratory syncytial virus circulating in Quanzhou, 2018-2019].

Jingdong Song Z.X. Chen Jianqiu Guo Zhiping Xie Jialiang Du

Objective: To study the prevalence and genetic characteristics of human respiratory syncytial virus (HRSV) in Quanzhou city, from 2018 to 2019. Methods: A total of 141 throat swabs were collected from children patients of lower respiratory tract infection in Quanzhou children Hospital, Fujian Province from November 2018 to May 2019. RT-PCR was used to amplify the 3 'end of G gene HRSV. Sequencer 5.0 and MEGA5.05 softwares were used for sequence editing, phylogenetic tree construction and genotyping analysis. Results: Twenty-five samples were positive for HRSV. Seventeen samples succeeded to obtain the target gene, including 13 of HRSVA and 4 of HRSVB. Two genotypes were identified: ON1 genotype (13 samples, HRSVA) and BA9 genotype (4 samples, HRSVB). Five strains of ON1 genotype sequences were clustered with the ON1 sequences prevalent in Beijing, Changchun and Zhejiang from 2012 to 2015 (cluster1); one strain (FJ19-02) was clustered with the sequences of ON1 genotype circulating in many regions of China from 2012 to 2015 (cluster2); Seven strains were clustered independently (cluster FJ). FJ18-02, FJ19-14 and FJ19-15 of HRSVB were clustered with the BA9 genotype sequences prevalent in Changchun, Jilin Province in 2015, while FJ19-13 was closely related to the BA9 genotype sequences prevalent in Guangzhou and Zhejiang Province in 2013. Both the ON1 and BA9 genotypes showed variations of nucleotide and amino acid in 72 and 60 insertion segments. Amino acid mutation (H266L) only occurred among the sequence of cluster-FJ, and the mutations of H261Q and Q265L only appeared in strain FJ19-13. Conclusion: BA9 and ON1 genotypes were prevalent in Quanzhou city, from 2018 to 2019. Cluster-FJ was a newly discovered independent transmission chain, which may continue to circulate in local Quanzhou area.

10.3760/cma.j.cn112137-20210202-00326

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Efficient Neutralization of SARS-CoV-2 Omicron and Other VOCs by a Broad Spectrum Antibody 8G3

bioRxiv (Cold Spring Harbor Laboratory) (2022)

Hang Ma Chien‐Te K. Tseng Huifang Zong Yunji Liao Yong Ke

Abstract Numerous mutations in the spike protein of SARS-CoV-2 B.1.1.529 Omicron variant pose a crisis for antibody-based immunotherapies. The efficacy of emergency use authorized (EUA) antibodies that developed in early SARS-CoV-2 pandemic seems to be in flounder. We tested the Omicron neutralization efficacy of an early B cell antibody repertoire as well as several EUA antibodies in pseudovirus and authentic virus systems. More than half of the antibodies in the repertoire that showed good activity against WA1/2020 previously had completely lost neutralizing activity against Omicron, while antibody 8G3 displayed non-regressive activity. EUA antibodies Etesevimab, Casirivimab, Imdevimab and Bamlanivimab were entirely desensitized by Omicron. Only Sotrovimab targeting the non-ACE2 overlap epitope showed a dramatic decrease activity. Antibody 8G3 efficiently neutralized Omicron in pseudovirus and authentic virus systems. The in vivo results showed that Omicron virus was less virulent than the WA1/2020 strain, but still caused deterioration of health and even death in mice. Treatment with 8G3 quickly cleared virus load of mice. Antibody 8G3 also showed excellent activity against other variants of concern (VOCs), especially more efficient against authentic Delta plus virus. Collectively, our results suggest that neutralizing antibodies with breadth remains broad neutralizing activity in tackling SARS-CoV-2 infection despite the universal evasion from EUA antibodies by Omicron variant.

2019-20 coronavirus outbreak

Broad spectrum

10.1101/2022.02.25.482049

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The epidemiological and genetic characteristics of human parvovirus B19 in patients with febrile rash illnesses in China

Scientific Reports (2023)

Haoran Jiang Qi Qiu Yangzi Zhou Yan Zhang Wenbo Xu

Abstract To understand the epidemiological and genetic characteristics of B19V, a multiple-province surveillance of patients with febrile rash illnesses (FRIs) were conducted in China during 2009 ~ 2021. The clinical specimens of 3,820 FRI patients were collected and tested for B19V DNA. A total of 99 (2.59%) patients were positive for B19V, and 49 (49.49%) were children under 5 years old. B19V infections occurred throughout the year without obvious seasonal pattern. Ten NS1-VP1u sequences and seven genome sequences were obtained in this study, identified as subgenotype 1a. Combined with the globally representative genome sequences, no temporal and geographic clustering trends of B19V were observed, and there was no significant correlation between B19V sequences and clinical manifestations. The evolutionary rate of the B19V genome was 2.30 × 10 –4 substitutions/site/year. The number of negative selection sites was higher than that of positive selection sites. It was the first to comprehensively describe the prevalence patterns and evolutionary characteristics of B19V in FRI patients in China. B19V played the role in FRI patients. Children under 5 years old were the main population of B19V infection. Subgenotype 1a was prevalent in FRI patients in China. B19V showed a high mutation rate, while negative selection acted on the genome.

10.1038/s41598-023-43158-y

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Citations (5)

Multiple-centre clinical evaluation of an ultrafast single-tube assay for SARS-CoV-2 RNA

Clinical Microbiology and Infection (2020)

J. Wang Kun Cai Xiaozhou He Xinxin Shen J. Wang

10.1016/j.cmi.2020.05.007

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Citations (52)

Glycopeptide ligation via direct aminolysis of selenoester

Chinese Chemical Letters (2018)

Jingjing Du Ling-Ming Xin Ze Lei Shi‐Yao Zou Wenbo Xu