Objective To construct the recombinant adenovirus vector encoding human nm23-H1 gene by AdEasy-1 adenovirus vector system.Methods A pair of DNA primers with KpnⅠand XhoⅠ restriction enzyme site were designed.Nm23-H1 cDNA was cloned from pMD18-T-nm23 plasmid by PCR technology and cloned into pShuttle-CMV in correct direction and the recombinant plasmid was called pShuttle-CMV-nm23-H1.It was identified by double digestion with KpnⅠand XhoⅠrestriction enzymes and DNA sequencing.The replication-defective adenovirus type 5 backbone plasmid pAdEasy-1 was transformed into competent bacteria BJ5183 by electroporation,pShuttle-CMV-nm23-H1 was linearized by PmeⅠand dephosphorylated and transformed into competent bacteria BJ5183 which contained pAdEasy-1 backbone plasmid.Then the product was selected by kanamycin and identified by digestion with PacⅠ.The recombinant adenovirus vector plasmid was amplified broadly in XL10-Gold competent bacteria and identified by PCR.Results A fragment size about 460bp was got by digestion with KpnⅠand XhoⅠ.Its sequence was identical to that of gene nm23-H1 by DNA sequencing.The recombinant advenoviral plasmid could produce a fragment whose size was 3.0kb or 4.5kb when digested with PacⅠ.The 460bp fragment was appeared after PCR identification.Conclusion The recombinant adenovirus plasmid encoding human nm23-H1 gene by AdEasy-1 adenovirus vector system was constructed successfully.
Abstract Background Atrial fibrillation (AF), as the most burdensome cardiac arrhythmia, could lead to the significant mortality and morbidity. Atrial fibrosis is a well-recognized pathophysiological factor of the development and progression of AF, which also hampers its treatment. However, the underlying mechanisms of this intricate process remain largely unknown. Purpose This study sought to reveal the cellular composition of AF substrate and investigate the vital contributors to atrial structural remodeling. Methods Left atrial appendages from three patients with AF undergoing surgical ablation and three individuals with sinus rhythm undergoing heart transplantation were subjected to 10X single-cell RNA sequencing (scRNA-seq). Another five public scRNA-seq or snRNA-seq datasets of potentially remodeled right or left appendages were retrieved across three publications. The vital contributors of atrial structural remodeling and their characteristics were identified by intercellular communication analysis and trajectory inference. Transverse aortic constriction (TAC) was used to induce atrial remodeling in Cdh5-CreERT2;RFP mice, followed by scRNA-seq of endothelial cells (ECs). Human primary atrial ECs and fibroblasts were isolated for cell-cell interaction experiments. ECs-specific mouse models of Transforming growth factor beta 1 (TGFB1) (knockout and overexpression) were generated. Electrophysiological and histology analyses were performed to determine the consequences of ECs-derived TGFB1 gain and loss of function in the atrium. Results The comprehensive analysis of different scRNA-seq datasets revealed that ECs played an important regulatory role in the homeostasis of atrial substrate and displayed remarkable mesenchymal activation during atrial remodeling, which was further confirmed by TAC mice. Immunofluorescence staining and high-content screening suggested that the complete transition from ECs to mesenchymal cells slightly contributed to atrial remodeling. However, TGFB1 secreted from mesenchymal activated ECs significantly promoted activation, proliferation, and collagen secretion of fibroblasts. We discovered that overexpression of TGFB1 in ECs of mice significantly increased atrial fibrosis, and thus prolonging AF duration. Besides, knockout of TGFB1 in ECs of mice underwent TAC significantly attenuated the atrial fibrosis and decreased the rate of AF. Conclusion Our work demonstrates that mesenchymal ECs-derived Tgfb1 plays an important role in atrial remodeling by regulating the fibroblast function. EC-specific interventions were suggested to facilitate the development of novel strategies for mitigating AF substrate remodeling.
Defect engineering constitutes a widely-employed method of adjusting the electronic structure and properties of oxide materials. However, controlling defects at room temperature remains a significant challenge due to the considerable thermal stability of oxide materials. In this work, a facile room-temperature lithium reduction strategy is utilized to implant oxide defects into perovskite BaTiO3 (BTO) nanoparticles to enhance piezocatalytic properties. As a potential application, the piezocatalytic performance of defective BTO is examined. The reaction rate constant increases up to 0.1721 min−1, representing an approximate fourfold enhancement over pristine BTO. The effect of oxygen vacancies on piezocatalytic performance is discussed in detail. This work gives us a deeper understanding of vibration catalysis and provides a promising strategy for designing efficient multi-field catalytic systems in the future.
Building an honest and clean government and serving the people diligently serve as the main guide line of Mao Ze-don‘gs theory on party and army building. From six aspects including education, system, supervision, working style, punishment and examples set by cadres, Mao Zedong considered and practiced his anti-corruption system, thus formed his own unique the-ory and successfully created an honest and upright society in New China. Facing increasingly complex international, domestic and era background, anticorruption struggle deserves our attention at present in our country. The enlightenment of Mao Ze-dong’s anti-corruption theory manifests the following six aspects:overcome the trend of formalism in education and increase its actual effect ; enforce the law strictly and enhance the enforcement power of disciplines; advocate democracy and construct comprehensive supervision system; pay attention to working styles and enhance the intimate relationship between the people and cadres; eradicate the‘special privilege’mentality and emphasize cadres’role models; increase the intensity of punishment and demonstrate the power of caution and awe.
Registration is essential for terrestrial LiDAR (light detection and ranging) scanning point clouds. The registration of indoor point clouds is especially challenging due to the occlusion and self-similarity of indoor structures. This paper proposes a 4 degrees of freedom (4DOF) coarse registration method that fully takes advantage of the knowledge that the equipment is levelled or the inclination compensated for by a tilt sensor in data acquisition. The method decomposes the 4DOF registration problem into two parts: (1) horizontal alignment using ortho-projected images and (2) vertical alignment. The ortho-projected images are generated using points between the floor and ceiling, and the horizontal alignment is achieved by the matching of the source and target ortho-projected images using the 2D line features detected from them. The vertical alignment is achieved by making the height of the floor and ceiling in the source and target points equivalent. Two datasets, one with five stations and the other with 20 stations, were used to evaluate the performance of the proposed method. The experimental results showed that the proposed method achieved 80% and 63% successful registration rates (SRRs) in a simple scene and a challenging scene, respectively. The SRR in the simple scene is only lower than that of the keypoint-based four-point congruent set (K4PCS) method. The SRR in the challenging scene is better than all five comparison methods. Even though the proposed method still has some limitations, the proposed method provides an alternative to solve the indoor point cloud registration problem.
In this paper we study symmetry properties of the Hilbert transformation of several real variables in the Clifford algebra setting. In order to describe the symmetry properties we introduce the group $r\mathrm{Spin}(n)+\mathbb{R}^n, r>0,$ which is essentially an extension of the ax+b group. The study concludes that the Hilbert transformation has certain characteristic symmetry properties in terms of $r\mathrm{Spin}(n)+\mathbb{R}^n.$ In the present paper, for $n=2$ and $3$ we obtain, explicitly, the induced spinor representations of the $r\mathrm{Spin}(n)+\mathbb{R}^n$ group. Then we decompose the natural representation of $r\mathrm{Spin}(n)+\mathbb{R}^n$ into the direct sum of some two irreducible spinor representations, by which we characterize the Hilbert transformation in $\mathbb{R}^3$ and $\mathbb{R}^2.$ Precisely, we show that a nontrivial skew operator is the Hilbert transformation if and only if it is invariant under the action of the $r\mathrm{Spin}(n)+\mathbb{R}^n, n=2,3,$ group.
The results suggest that CaMBP-10 is a novel member of plant nsLTPs family, the Ca~(2+)/CaM messenger system is involved in the function of lipid transfer protein.
In this paper a technical way to take minitor and control to the total amount of the polling source in industrial waste water is proposed in accordence with the real conditions of the discharge of industrial waste water at presant and the capability of the technique and equipment in three classed environmental monitoring centers. moreover, a practical method to monitor and control the total amount of industrial waster water by scientific samples and analysis in laboratory is discussed.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)