Dongjun Liu

Inner Mongolia University

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Ming Cang

Inner Mongolia University

Zhigang Wang

Ocean University of China

Hao Liang

Vanderbilt University Medical Center

Haiqing Wu

AviChina Industry & Technology (China)

Bai Dai

Inner Mongolia University

Yuzhen Ma

Inner Mongolia People's Hospital

Jianlong Yuan

Shanghai Jiao Tong University

Nuo MingTu

Inner Mongolia University

Yu Ren

Chinese Academy of Medical Sciences & Peking Union Medical College

Xiaoyu Han

Union Hospital

Cooperative Institutions

Inner Mongolia University

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Peking University

Inner Mongolia Medical University

Capital Medical University

University of Chinese Academy of Sciences

Jilin University

Shanghai Jiao Tong University

Nanjing Medical University

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Effects of Crotonylation on Reprogramming of Cashmere Goat Somatic Cells with Different Differentiation Degrees

Animals (2022)

Wennan Li Wei Yan Hao Fei Lingyun Hao Dongjun Liu

Failure in the epigenetic reprogramming of somatic cells is considered the main reason for lower cloned embryo development efficiency. Lysine crotonylation (Kcr) occupies an important position in epigenetic modification, while its effects on somatic cell reprogramming have not been reported. In this study, we detected the influence of sodium crotonate (NaCr) on the Kcr levels in three types of somatic cells (muscle-derived satellite cells, MDSCs; fetal fibroblast cells, FFCs; and ear tip fibroblast cells, EFCs). The three types of somatic cells were treated with NaCr for cloned embryo construction, and the cleavage rates and Kcr, H3K9cr, and H3K18cr levels in the cloned embryos were analyzed. The results showed that the abnormal levels of Kcr, H3K9cr, and H3K18cr were corrected in the treatment groups. Although there was no significant difference in the cloned embryo cleavage rate in the FFC treatment group, the cleavage rates of the cloned embryos in the MDSCs and EFCs treatment groups were increased. These findings demonstrated that the Kcr level was increased with NaCr treatment in somatic cells from Cashmere goat, which contributed to proper reprogramming. The reprogramming of somatic cells can be promoted and cloned embryo development can be improved through the treatment of somatic cells with NaCr.

Reprogramming

Cleavage (geology)

10.3390/ani12202848

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Citations (2)

BIX-01294 promotes the differentiation of adipose mesenchymal stem cells into adipocytes and neural cells in Arbas Cashmere goats

Research in Veterinary Science (2018)

Qing Wang Xiao Wang Defang Lai Jinhai Deng Zhuang Hou

Homeobox protein NANOG

10.1016/j.rvsc.2018.05.009

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Citations (14)

MSTN遺伝子の発現を調節するためにトランスフェクトしたFST遺伝子ヒツジ筋Drived筋原細胞【Powered by NICT】

Zhongguo Nongye Kexue (2013)

Yue Qunhua Jianlong Yuan Hao Fei Jin Muzi Wang Jingyuan

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Factors Affecting the Derivation and Expansion of Bovine Embryonic Stem Cell-Like Cells

Journal of Animal and Veterinary Advances (2012)

Rui Wang Shan Cong Ming Cang Yuzhen Ma Jianxun Wen

10.3923/javaa.2012.2865.2872

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[Production of transgenic embryos through nuclear transfer using ovine fetal fibroblasts transferred with foreign genes].

PubMed (2011)

Yuzhen Ma Yu Ren Xueyuan Zhou Dongjun Liu Xu Rigan

Human ALR gene sequence was amplified by PCR from human total DNA and inserted into pIRES(2)-EGFP vector. The bicistronic eukaryotic expression vector, pIRES-EGFP/ALR, expressing EGFP, Neo(r) and ALR genes was constructed. Sheep fetal fibroblast cells (sEFCs) were transfected with pIRES-EGFP/ALR by the induction of lipofectAMINE(TM). The positive cell clones were selected with medium containing G418 (800 μg/mL). The fluorescence of transgenic cells was examined with a confocal laser scanning microscope. The expression of ALR gene was tested by PCR, RT-PCR and immuno-histochemical staining. The transgenic cells were used as donors for nuclear transfer to enucleated ovine oocytes. Transgenic embryos were tested by confocal laser scanning microscope and immuno-histochemical staining. Results showed that the EGFP and ALR genes linked with IRES were coexpressed simultaneously in sFFCs; the blastocysts formed by nuclear transfer using tranfected donor cells are all transgenic blastocysts. EGFP, ALR and Neo(r) gene were all expressed in the transgenic embryos. In conclusion that a method to construct the positive embryos before pre-implantation which stably express ALR gene by the indication of EGFP expression has been successfully established. The application of this method can simplify the procedure of testing the targets and contribute to the efficiency increasing of transgenic domestic animal production.

Lipofectamine

10.3724/sp.j.1141.2011.06617

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GT-KGCN: Gene Essentiality Prediction with Graph Transformer and Kolmogorov-Arnold Network

2021 IEEE International Conference on Bioinformatics and Biomedicine (BIBM) (2024)

R S Liu Bing Jia Baoqi Huang Winston K.G. Seah Fei Hao

10.1109/bibm62325.2024.10822036

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The mammalian target of rapamycin signaling pathway regulates myocyte enhancer factor‐2C phosphorylation levels through integrin‐linked kinase in goat skeletal muscle satellite cells

Cell Biology International (2015)

Haiqing Wu Yu Ren Wei Pan Zhenguo Dong Ming Cang

Mammalian target of rapamycin (mTOR) signaling pathway plays a key role in muscle development and is involved in multiple intracellular signaling pathways. Myocyte enhancer factor-2 (MEF2) regulates muscle cell proliferation and differentiation. However, how the mTOR signaling pathway regulates MEF2 activity remains unclear. We isolated goat skeletal muscle satellite cells (gSSCs) as model cells to explore mTOR signaling pathway regulation of MEF2C. We inhibited mTOR activity in gSSCs with PP242 and found that MEF2C phosphorylation was decreased and that muscle creatine kinase (MCK) expression was suppressed. Subsequently, we detected integrin-linked kinase (ILK) using MEF2C coimmunoprecipitation; ILK and MEF2C were colocalized in the gSSCs. We found that inhibiting mTOR activity increased ILK phosphorylation levels and that inhibiting ILK activity with Cpd 22 and knocking down ILK with small interfering RNA increased MEF2C phosphorylation and MCK expression. In the presence of Cpd 22, mTOR activity inhibition did not affect MEF2C phosphorylation. Moreover, ILK dephosphorylated MEF2C in vitro. These results suggest that the mTOR signaling pathway regulates MEF2C positively and regulates ILK negatively and that ILK regulates MEF2C negatively. It appears that the mTOR signaling pathway regulates MEF2C through ILK, further regulating the expression of muscle-related genes in gSSCs.

Mef2

MEF2C

Integrin-linked kinase

10.1002/cbin.10499

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Citations (6)

Whole-genome resequencing of Ujumqin sheep to investigate the determinants of the multi-vertebral trait

Genome (2018)

Shuo Li Rongsong Luo Defang Lai Min Ma Fei Hao

The Ujumqin sheep is one of the most profitable breeds in China, with unique multi-vertebral characteristics. We performed high-throughput genome resequencing of five multi-vertebral and three non-multi-vertebral sheep in an Ujumqin population. We identified the genomic regions that correlated with the germplasm characteristics to establish the cause of the "multi-vertebral" phenotype in this breed. Sequencing generated a total of 314 952 000 000 bp of raw data. The alignment rate of all the samples was between 98.53% and 99.11%, and the mean depth of coverage relative to the reference genome was between 11.58× and 14.92×. After comparing the differences between the two groups, we identified 21 homozygous single nucleotide polymorphisms (SNPs) in the mutant exons of 14 genes. Nineteen loci of 10 genes contained nonsynonymous mutations, while two loci contained synonymous mutations. Resequencing revealed homozygous mutations comprised of 44 indels located within exons of 19 genes. These indels included 37 frameshift mutations, 6 non-frameshift mutations, and 1 stopgain single nucleotide variation (SNV). Finally, comparisons of genotypic variations revealed 17 genes with homozygous mutations in their coding regions, 5 of which have previously been associated with vertebral development and the remaining 12 genes were newly identified in this study.

Indel

Nonsynonymous substitution

INDEL Mutation

10.1139/gen-2017-0267

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Citations (12)

Observation of Morphology and Proliferative Density for Cashmere Goat Fetal Fibroblasts after mTOR Inhibition with Optical Microscope Cell Imaging

Xiaojing Wang Manlin Wu Yan Liang Zhigang Wang Dongjun Liu

The mammalian target of rapamycin (mTOR) is a kind of Ser/Thr kinase in mammalian cells. It can recruit and integrate input signals from modulators such as nutrients and growth factors to regulate cell growth and proliferation via different cellular processes. The mTOR signaling pathway also affects the development of the embryonic stem cells (ESCs) and early embryo, and involved in cancers and metabolism disease. The mTOR signaling pathway has been investigated extensively in human, mouse and rat, whereas it is not yet studied in goat due to the lack of basic data about genes, gene expression and function. Thus this study uses the CCI-779, an mTOR specific inhibitor, treating Inner Mongolia Cashmere goat fetal fibroblasts (GFb) to prove that the mTOR signaling pathway acts as regulator in the cell proliferation. The GFb cells were treated with different concentrations of CCI-779 (0.1 μM, 1.0 μM, 5.0 μM, 10.0 μM and 20.0 μM) for 24h and 48h, and then the cell shape and proliferative density were observed with optical microscopy cell imaging. The results showed that GFb cells were sensitive to CCI-779. CCI-779 inhibits the cell proliferation. Dead Cells floating on medium were clearly observed after 10 μM and 20 μM CCI-779 treatments for 24h. Some cells' shape changed to round from typical shape after treatment with 20μM CCI-779 for 48h. The Morphological results indicated that mTOR is functional in GFb cells and acts as a key kinase to regulate cell proliferation. The synthesis of microfilament or organization of cytoskeleton perhaps was disrupted in GFb cells when mTOR was inhibited. Taken together, the data showed that the mTOR signaling pathway plays a critical role in the GFb cell proliferation.

10.1109/icbbe.2011.5780115

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Comparison of gene editing efficiencies of CRISPR/Cas9 and TALEN for generation of MSTN knock-out cashmere goats

Theriogenology (2019)

Ju Zhang Jian Liu Wenliang Yang Meng‐Lan Cui Bai Dai

Nuclease

10.1016/j.theriogenology.2019.03.029

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Citations (37)