Hemoglobin concentration, as an important indicator of the recovery of the surgical site of the flap graft, can reflect the state of blood circulation. Current methods for monitoring blood flow after surgery are limited to physical observations and empirical diagnosis of skin temperature, color, and swelling, or only rough determination of oxygen saturation, and less to realtime monitoring of hemoglobin concentration. In this paper, a multi-directional reflective non-invasive test system for detecting hemoglobin concentration after skin flap transplantation was designed and constructed. Through the collection and analysis of sample data, a correction factor was introduced to achieve noninvasive detection of hemoglobin concentration. The experimental results showed that the standard deviation of 10 consecutive measurements of 6 volunteers was relatively small; the correlation between 40 measurement value and true value was relatively strong; the concentration of hemoglobin collected after skin heating was sensitive to temperature changes, indicating that the system has good stability, accuracy and effectiveness. The project team will cooperate with the Huaihe Hospital of Henan University to conduct clinical testing of hemoglobin concentration in patients after flap transplantation, further calibrate the data and develop miniaturized portable measuring instruments to provide more reliable basis for postoperative care.
Propamocarb (PM), a carbamate fungicide, can effectively control downy mildew on cucumber. However, due to the large-scale and high-dose use of this fungicide, PM residues have become a major problem in cucumber production. In this report, the cucumber cultivar "D0351" (with the lowest residual PM content) and the cucumber cultivar "D9320" (with the highest residual PM content) were used as experimental materials. The candidate gene CsMCF, which is related to a low residual PM content in cucumber, was screened by high-throughput tag-sequencing (Tag-Seq) and PM analysis, and its role in reducing PM residue in cucumber was explored. CsMCF was cloned and obtained. This gene contains an open reading frame of 1026 bp, encodes 341 amino acids and contains 3 Mito-carr domains. The encoded protein is a hydrophobic protein with 4 distinct transmembrane structures but no signal peptide cleavage sites. The subcellular localization of the protein is the cytoplasm. Evolutionary tree analysis showed that CsMCF had the highest homology to a gene from the melon Cucumis melo L. (XM_008464998.2). The core elements of the promoter include cis-acting elements, such as those related to salicylic acid (SA), jasmonic acid (JA), gibberellin (GA), and abscisic acid (ABA). Following PM treatment, CsMCF was significantly upregulated at most time points in different parts of the fruit, leaf, stem and root of "D0351," while expression was downregulated at most time points in the fruit, leaf and stem of "D9320." The order of the expression levels in different cucumber organs was as follows: fruit>leaf > stem > root. CsMCF was specifically expressed in the stems and leaves of "D0351." The PM residues in CsMCF (+)-overexpressing T0 and T1 cucumber fruits were significantly lower than those in the wild type, while the PM residues in CsMCF (-)-overexpressing T0 and T1 cucumber fruits were significantly higher. The qRT-PCR results showed that CsMCF can respond to biotic and abiotic stresses, actively respond to PM treatment and play a role in reducing PM residues in cucumber fruits.
All plants in the core region of Zhongwei goat were researched by large samples that were located in mountainous grassland at different altitude and grazing pressure,and the data of research results were calculated by four β diversity indices. There are 151 plants that belong to 39 families and 95 genera in the mountainous desert grassland. It is different that the dynamic state of different species in every month(from May to November), and dominant species changed apparently in every month in different samples. The results of β diversity and numbers of species of every samples reflected the synthesis effect of topographical diversity ,water and sunbeam distribution,interference, and the effect of the topographical diversity significantly. Heavy interference (including grazing, reclamation, mining etc) aggravated the no-completeness of ecosystem of desert grassland, which led the chaos of construction inner the ecosystem directly, and then drove the paradox among every productive layer of mountainous desert grassland ecosystem, furthermore the chronicity and circularity of those effects made the grassland ecosystem more degradation. In the end, the author suggested that the government should seal and nurse the grassland ecosystem to improve the completeness of the ecosystem.
Abstract Complete disruption of critical genes is generally accompanied by severe growth and developmental defects, which dramatically hinder its utilization in crop breeding. Identifying subtle changes, such as single-nucleotide polymorphisms (SNPs), in critical genes that specifically modulate a favorable trait is a prerequisite to fulfill breeding potential. Here, we found 2 SNPs in the E-class floral organ identity gene cucumber (Cucumis sativus) SEPALLATA2 (CsSEP2) that specifically regulate fruit length. Haplotype (HAP) 1 (8G2667A) and HAP2 (8G2667T) exist in natural populations, whereas HAP3 (8A2667T) is induced by ethyl methanesulfonate mutagenesis. Phenotypic characterization of 4 near-isogenic lines and a mutant line showed that HAP2 fruits are significantly longer than those of HAP1, and those of HAP3 are 37.8% longer than HAP2 fruit. The increasing fruit length in HAP1-3 was caused by a decreasing inhibitory effect on CRABS CLAW (CsCRC) transcription (a reported positive regulator of fruit length), resulting in enhanced cell expansion. Moreover, a 7638G/A-SNP in melon (Cucumis melo) CmSEP2 modulates fruit length in a natural melon population via the conserved SEP2-CRC module. Our findings provide a strategy for utilizing essential regulators with pleiotropic effects during crop breeding.
Metagenome is a fast developing cultivation independent technology, which is useful in addressing evolutionary and ecological relationship of microbial communities, also quite challenging to research function genes. Here we improved DNA extraction method and the DNA extracted is of high yield and allows direct 16S rDNA PCR amplification. we used the high-molecular-weight bacterial DNA to construct sediment metagenomic library. The metagenomic library of river sediments polluted was constructed, and then was estimated by digested clones selected randomly by BamH I. The metagenomic library containing 6,000 clones was constructed into pUC18 vector. An positive clones was selected by functional screening of the library. A Zn-Cd resistance active gene designated Mtx was identified by subcloning, sequencing and expression. This Mtx gene encodes cation-proton-antiporter belonging to Czc family, which is most related to the Czc R gene from Ralstonia sp. CH34 with 93% similarity.
This paper studies the external technical support and intelligent system construction of our green development under the background of the Internet.First, with the environmental problems existing in economic construction as the background, based on the current situation of the Internet development in the region, it analyzes the green development from the agricultural Internet and the industrial Internet., Energy Internet and "Internet +" feature extraction from four perspectives, based on intelligent algorithms to explore green development paths, and intelligent system modeling to improve environmental quality and achieve high-quality economic development.
Objective This study aimed to utilize data from the China family panel studies (CFPS) to systematically analyze the physical and mental health characteristics and needs of older adult individuals with disabilities under the context of smart healthcare, aiming to provide scientific evidence for relevant policy formulation. Methods Data were derived from the CFPS surveys conducted by Peking University’s China Social Sciences between 2021 and 2022. Older adult individuals aged 65 and above requiring assistance in activities of daily living (ADL) or instrumental activities of daily living (IADL) were included. Descriptive statistics, univariate analysis, and multivariate analysis were employed to analyze the basic characteristics, physical and mental health status, influencing factors, and corresponding needs of older adult individuals with disabilities. Results Samples aged between 65 and 80, widowed or divorced, demonstrated higher physical health scores ( p < 0.05); samples with education at college level or above, residing in urban areas, free from chronic diseases, with annual income exceeding 100,000 RMB, receiving daily family support, frequently accessing community services, and regularly using smart medical devices exhibited greatly higher levels of physical health, mental health, and overall scores on the short form health survey (SF-36) ( p < 0.05). Factors such as younger age, absence of chronic diseases, higher economic status, daily family support, frequent access to community services, and regular use of smart medical devices exerted favorable effects on the physical health status of older adult individuals with disabilities ( p < 0.05); absence of chronic diseases, urban residence, higher economic status, daily family support, and frequent access to community services were found to positively influence the mental health status of older adult individuals with disabilities ( p < 0.05). Conclusion The physical and mental health status of older adult individuals with disabilities is influenced by various factors, including social support, economic conditions, and utilization of medical resources. These factors constitute significant determinants in improving the physical and mental health of older adult individuals with disabilities and represent key needs for their betterment.
In this study,wild melon ‘LCW-01’ was chosen for male and cultivated melon ‘X09-18’ for female.Then,the branch number,fruit-bearing rate,single fruit weight et al.20 total major characteristics of the hybrid progeny were observed.ADM model was used to genetic analysis and study the wild hybrid progeny inheritance.The results showed that the hybrid F1 was tended to cultivated parent.The different results of positive and negative F1-hybrid progeny showed that there existed maternal effects.Placenta color and fresh bitter taste complied to mendelism and controlled by one or two pair dominant genes respectively,white to orange as bitter to not bitter was quantities trait.Flesh color and acidity were qualitative traits,controlled by many genes.Whereas the rest traits,branch number for example,were quantitative traits.Because of different traits so the genetic variance components and genetic force size were different.
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