Objective Elevated platelet count (PC), a measure of systemic inflammatory response, is inconsistently reported to be associated with poor prognosis in patients with renal cell carcinoma (RCC). We conducted a systematic review and meta-analysis to clarify the significance of PC in RCC prognosis. Methods PubMed, Embase, and Web of Science databases were searched to identify eligible studies to evaluate the associations of PC with patient survival and clinicopathological features of RCC. Results We analyzed 25 studies including 11,458 patients in the meta-analysis and categorized the included articles into three groups based on RCC stage. An elevated PC level was associated with poor overall survival (OS, hazard ratio [HR] 2.24, 95% confidence interval [CI] 1.87-2.67, P<0.001) and cancer-specific survival (CSS, HR 2.59, 95% CI 1.92-3.48, P<0.001) when all stages were examined together; with poor CSS (HR 5.09, 95% CI 2.41-10.73, P<0.001) and recurrence-free survival (HR 6.68, 95% CI 3.35-13.34, P<0.001) for localized RCC; with poor OS (HR 2.00, 95% CI 1.75-2.28, P<0.001) for metastatic RCC; and with poor OS (HR 2.05, 95% CI 1.04-4.03, P = 0.038), CSS (HR 3.38, 95% CI 1.86-6.15, P<0.001), and PFS (HR 2.97, 95% CI 1.47-6.00, P = 0.002) for clear cell RCC. Furthermore, an elevated PC level was significantly associated with TNM stage (OR 3.11, 95% CI 1.59-6.06, P = 0.001), pathological T stage (OR 3.13, 95% CI 2.60-3.77, P<0.001), lymph node metastasis (OR 4.01, 95% CI 2.99-5.37, P<0.001), distant metastasis (OR 3.85, 95% CI 2.46-6.04, P<0.001), Fuhrman grade (OR 3.70, 95% CI 3.00-4.56, P<0.001), tumor size (OR 4.69, 95% CI 2.78-7.91, P<0.001) and Eastern Cooperative Oncology Group score (OR 5.50, 95% CI 3.26-9.28, P<0.001). Conclusion An elevated PC level implied poor prognosis in patients with RCC and could serve as a readily available biomarker for managing this disease.
Abstract MicroRNA‐205 (miR‐205) is involved in various physiological and pathological processes, but its biological function in follicular atresia remains unclear. In this study, we investigated miR‐205 expression in mouse granulosa cells (mGCs) and analyzed its functions in primary mGCs by performing a series of in vitro experiments. Quantitative real‐time polymerase chain reaction showed that miR‐205 expression was significantly higher in early atretic follicles and progressively atretic follicles than in healthy follicles. miR‐205 overexpression in mGCs significantly promoted apoptosis and caspase‐3/9 activities, as well as inhibited estrogen (E2) release and cytochrome P450 family 19 subfamily A polypeptide 1 (CYP19A1, a key gene in E2 production) expression. Bioinformatics and luciferase reporter assays revealed that the gene encoding cyclic AMP response element (CRE)‐binding protein 1 (CREB1) was a direct target of miR‐205 in mGCs. CREB1 upregulation partially rescued the effects of miR‐205 on apoptosis, caspase‐3/9 activities, E2 production, and CYP19A1 expression on mGCs. These results indicate that miR‐205 might play an important role in ovarian follicular development and provide new insights into follicular atresia
Abstract We propose to fabricate a bifunctional photocatalyst for cooperative glycerol selective oxidation and H 2 evolution. The key role of interface scale (nanometer, subnanometer, or atomic level) on tuning catalysis performance was revealed. Specifically, series Pt/defective TiO 2 with tailored interface scale were constructed by loading Pt nanoparticle, cluster, and single atom on V Ti ‐enriched TiO 2 . It was uncovered that reducing interface scale could largely improve the visible‐light utilization efficiency, the photoexcited electron–hole separation, and well tuned the band structure. It could also enhance the metal–support interaction and thus adjust the chemical behavior toward reactant molecules. The optimized isolated Pt/defective TiO 2 with atomic‐level interface achieved 90.6% glycerol conversion and 87.2% C 3 products selectivity, accompanied by 7250.4 μmol g −1 H 2 accumulative yield. It also exhibited long‐term stability of 36 h without performance loss. Besides, the dominate active site was identified and the possible reaction mechanism was illustrated combined with Density Functional Theory (DFT) calculation and quasi in situ experiments.
Abstract Background Atrial fibrillation (AF) is a prevalent and chronic cardiovascular disorder associated with various pathophysiological alterations, including atrial electrical and structural remodeling, disrupted calcium handling, autonomic nervous system dysfunction, aberrant energy metabolism, and immune dysregulation. Emerging evidence suggests that long non-coding RNAs (lncRNAs) play a significant role in the pathogenesis of AF. Objective This discussion aims to elucidate the involvement of AF-related lncRNAs, with a specific focus on their role as miRNA sponges that modulate crucial signaling pathways, contributing to the progression of AF. We also address current limitations in AF-related lncRNA research and explore potential future directions in this field. Additionally, we summarize feasible strategies and promising delivery systems for targeting lncRNAs in AF therapy. Conclusion In conclusion, targeting AF-related lncRNAs holds substantial promise for future investigations and represents a potential therapeutic avenue for managing AF.
A new strategy for the size-controlled preparation of magnetic iron oxide nanocrystals (NCs) within hyperbranched poly(ethylenimine)s (HPEIs) has been described. HPEI was not only utilized as the nanoreactors and stabilizers to prepare size-controlled magnetic NCs, but also skillfully used as a base supplier to avoid introducing alkali hydroxide or ammonia. By changing the weight ratio of FeSO4·7H2O to HPEI, magnetic iron oxide NCs with various sizes were obtained. Owing to the efficient gene transfection properties of HPEI, the resulting iron oxide/HPEI nanocomposites were used as magnetic nonviral gene vectors for magnetofection and showed to be good gene vectors. It was found that the size of magnetic iron oxide had a significant effect on the magnetofection properties while a pure HPEI transfection enhancer was not introduced. When the mean size of magnetic iron oxide increased, the transfection efficiency was enhanced. With the addition of a pure HPEI transfection enhancer, the size of magnetic iron oxide showed a slight impact on the magnetofection properties. The luciferase expression levels mediated by iron oxide/HPEI nanocomposites with various iron oxide sizes in COS-7 cells under a magnetic gradient field were all more than 115 fold of that of standard HPEI transfection.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)