To explore targets of Chinese herbal medicine at cellular and molecular leve1s through an experimental study on Yinxingye Capsule (YC) intervening vascular endothelial cell apoptoeis of hyperhornocysteinemia (HHcy) rats.The HHcy model was prepared in male Wistar rats. Totally 42 rats were randomly divided into 4 groups, i.e., the control group (n =10), the model group (n = 11), the YC group (n =11), the folic acid group (n =10). Carboxy methyl cellulose (CMC) solution (1%) was administered to rats in the control group by gastrogavage.3% methionine suspension at 1. 5 g/kg was administered to rats in the model group by gastrogavage. 3% methionine suspension at 1. 5 g/kg and folic acid suspension at 0. 06 g/kg was administered to rats in the folic acid group by gastrogavage. 3% methionine suspension at 1. 5 g/kg and YC at 0. 02 g/kg was administered to rats in the YC group by gastrogavage. Morphological changes of aortic tissue were observed by hematoxylin eosin (HE) staining. The plasma homocysteine (Hcy) level was detected in each group. The endothelium-dependent diastolic functions of the thoracic aorta on different concentrations of sodium nitroprusside (SNP) and acetylcholine (Ach) were detected. Gene expressions of Bcl-2-associated X protein (BAX), inducible nitric oxide synthase (iNOS), c-Fos, cellular inhibitor of apoptosis protein 2 (c-IAP2) were detected by real time polymerase chain reaction (RT-PCR).Pathological results showed that thickening aortic endothelium, swollen and desquamated endothelial cells. Few foam cells could be seen in the model group. Myoma-like proliferation of smooth muscle cells in tunica media could also be seen. These pathological changes were milder in the YC group and the folic acid group. Compared with the control group, plasma Hcy levels increased in the model group (P <0. 05). The endothelium-dependent diastolic rates at 10(-6) and 10(-4)mol/L Ach and 10(-7) -10(-3)mol/L SNP all decreased in the model group (P <0. 01, P <0. 05). Gene expressions of Bax, c-Fos, and iNOS increased, but c-IAP2 gene expressions decreased in the model group (all P <0. 05). Compared with the model group, plasma Hcy levels decreased in the YC group and the folic acid group (P <0. 05). The endothelium-dependent diastolic rates increased in the YC group and the folic acid group at various SNP concentrations except 10(-6) mol/L SNP in the folic acid group. The endothelium-dependent diastolic rates increased in the YC group and the folic acid group at 10(-6) and 10(-4)mol/L Ach (all P <0. 05). Gene expressions of Bax, c-Fos, and iNOS decreased in the YC group and the folic acid group, but c-IAP2 gene expression increased in the folic acid group (all P <0. 05).YC could reduce plasma Hcy levels, down-regulate gene expressions of Bax, c-Fos, and iNOS, thereby reducing apoptosis of vascular endothelial cells, improving vascular endothelial function, and delaying atherosclerotic process.
To investigate the correlation between single-nucleotide polymorphism (SNP) of ARID5B gene and resistance to methotrexate (MTX) in children with acute lymphoblastic leukemia (ALL).A total of 144 children with ALL who were treated in General Hospital of Ningxia Medical University from January 2015 to November 2021 were enrolled and divided into MTX resistant group and non-MTX resistant group, with 72 cases in each group. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) technology was used to measure the SNP of ARID5B gene in all children and analyze its correlation with MTX resistant.There were no significant differences in the genotype and gene frequency of rs7923074, rs10821936, rs6479778, and rs2893881 between MTX resistant group and non-MTX resistant group (P>0.05). The frequency of C/C genotype in the MTX resistant group was significantly higher than that in the non-MTX resistant group, while the frequency of T/T genotype was opposite (P<0.05). The frequency of C allele in the MTX resistant group was significantly higher than that in the non-MTX resistant group, while the frequency of T allele was opposite (P<0.05). Multivariate logistic regression analysis showed that ARID5B gene rs4948488 TT genotype and T allele frequency were risk factors for MTX resistant in ALL children (P<0.05).The SNP of ARID5B gene is associated with MTX resistant in ALL children.ARID5B 基因SNP与ALL患儿MTX耐药性的相关性.探讨ARID5B 基因单核苷酸多态性（SNP）与急性淋巴细胞白血病（ALL）患儿甲氨蝶呤（MTX）耐药性的相关性。.纳入2015年1月至2021年11月于宁夏医科大学总院治疗的144例ALL患儿，分为MTX耐药组和非MTX耐药组，每组72例，采用基质辅助激光解吸电离飞行时间质谱技术测量所有患儿的ARID5B 基因SNP，分析其与患儿MTX耐药性的相关性。.两组患儿rs7923074、rs10821936、rs6479778、rs2893881位点基因型及基因频率比较无统计学差异（P>0.05）；MTX耐药组C/C基因型频率明显高于非MTX耐药组，而T/T基因型频率则相反（P<0.05）；MTX耐药组C等位基因频率明显高于非MTX耐药组，而T等位基因频率则相反（P<0.05）；多因素Logistic回归分析结果显示，ARID5B 基因rs4948488 TT基因型、T等位基因频率是ALL患儿MTX耐药性的危险因素（P< 0.05）。.ARID5B 基因SNP与ALL患儿MTX耐药性具相关性。.
In order to study the observation of postoperative vital sign data of critically ill patients, this paper developed a set of vital sign data acquisition system based on intelligence and realized the design of vital sign data acquisition system APP based on Android Studio programming software, which was used to realize the information collection of vital sign data of patients. PDA is connected with a vital sign measuring equipment through Bluetooth. The patient’s wristband is scanned with PDA to read the patient’s information, and then the measured vital sign data are obtained automatically by measuring APP. The initial alarm value is set to be greater than 1%, and it needs more than 60 and less than or equal to 120 RR interval data to judge apnea. Information collection of intelligent vital sign detection can not only save the time of nursing staff but also improve the nursing quality.
Objective To optimize the program for prevention and treatment of renal insufficiency of diabetic nephropathy (DN) and to evaluate the effectiveness and safety of the program of TCM treatment based on syndrome differentiation for protection of renal function.Methods With method of randomized,blind,controlled and multi—central clinical study,221 cases of renal insufficiency of DN were divided into group A (TCM treatment based on syndrome differentiation group),group B (Losartan group) and group C (TCM treatment plus Losartan treatment group).The treatment course was 3 months.Results After treatment,the increasing amplitude of serum creatimine clearance rate (Ccr) in group A was higher than in group B(P0.05),and the decreasing amplitude of serum creatimine(SCr) in group A was higher than in group B(P0.01) and C(P0.05);There was no significant difference in blood urea nitrogen (BUN) and 24—hour urinary protein among the 3 groups(P0.05).After treatment of 3 months,serum alanine aminotransferase (ALT),electrocardiogram(ECG) and B—ultrasonic examination results of the liver,gallbladder,spleen and pancreas did not have significant change(P0.05) with on significant difference among the 3 groups(P0.05).Conclusion The TCM treatment program in addition to diet regulation,decreasing blood sugar and expectant treatment iseffective and safe in improvingrenal function,and the program is better than Losartan in increasing Ccr and decreasing SCr.
Normally, there are multiple microRNAs involved in the pathogenesis of liver fibrosis. In our work, we aimed at identifying the role of miR-34c in the hepatic stellate cell (HSC) activation and liver fibrosis and its potential mechanism. Our results have shown that during natural activation of HSC, the level of miR-34c was increased significantly whereas acyl-CoA synthetase long-chain family member-1(ACSL1), which is a key enzyme can affect fatty acid(FA) synthesis, was decreased. A double fluorescence reporter assay further confirmed that ACSL1 is a direct target gene of miR-34c. Moreover, the inhibition of miR-34C can attenuate the synthesis of collagen in HSC-T6. In our rescue assay, ACSL1 expression was 1.49-fold higher compared to normal control cells which were transfected with the miR-34c inhibitor in a stable low expression ACSL1 cell line. While at the same time, α-SMA and Col1α expression decreased by 18.22% and 2.58%, respectively. Moreover, we performed an
The icing of the aeroengines caused by ice crystals has gradually become a research hotspot in recent years. In this study, the influence laws of different factors of ice melting on melting process of ice crystal were studied. Performed unsteady simulation of ice crystal melting under forced convection of air based on VOF method coupled with solidification and melting model in CFD simulation analysis. The influence laws of different factors of ice melting on melting process of ice crystal were revealed.
Augmented Reality is a kind of technique combining a virtual world with a real environment. This paper tells that OpenCV is applied to the realization of Registration Technique which is key to Augmented Reality. It studies the implementation of the camera calibration, the extraction of characteristic target and its recognition. This paper improved the efficiency of the exploitation and laid a foundation for the exploitation and application of Augmented Reality.
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