Ziwei Yu

Northwestern Polytechnical University

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David L. Rimm

Yale University

Diane Kowalski

The Maria Sklodowska-Curie National Research Institute of Oncology

Paul Weinberger

Louisiana State University Health Sciences Center Shreveport

Amanda Psyrri

National and Kapodistrian University of Athens

Clarence T. Sasaki

Yale University

Robert L. Camp

Yale University

Bruce G. Haffty

Rutgers, The State University of New Jersey

Pin Dong

Shanghai First People's Hospital

MΑ

Meletios Α. Dimopoulos

Alexandra Hospital

S. Markakis

National and Kapodistrian University of Athens

Cooperative Institutions

Shanghai Jiao Tong University

Chinese Academy of Sciences

Shanghai First People's Hospital

Yale University

Shanghai Ninth People's Hospital

Shanghai Sixth People's Hospital

National and Kapodistrian University of Athens

China Medical University

Shihezi University

University of Chinese Academy of Sciences

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Analysis of p53 protein expression levels on ovarian cancer tissue microarray using automated quantitative analysis elucidates prognostic patient subsets

Annals of Oncology (2007)

Amanda Psyrri Panteleimon Kountourakis Ziwei Yu C. Papadimitriou S. Markakis

Tissue microarray

10.1093/annonc/mdl479

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Citations (28)

Molecular prognostic markers in oropharyngeal squamous cell carcinoma; the role of C-met

Journal of Clinical Oncology (2004)

Ziwei Yu Paul Weinberger Diane Kowalski Bing Chang C T Sasaki

5517 Background: The c-Met oncogene encodes a transmembrane receptor which binds hepatocyte growth factor/scatter factor and exhibits intracellular tyrosine kinase activity. Activating mutations and constitutive overexpression of c-Met have been found in many cancers. One of the downstream mediators of c-Met signaling may be cyclooxygenase-2 (Cox-2) via the MAP-kinase pathway. We have previously reported prognostic significance for Cox-2 expression in oropharyngeal squamous cell cancer (SCC). In the present study we investigated the relationship between c-Met expression and outcome in patients with SCC of the oropharynx. We also compared c-Met with previously determined Cox-2 expression levels. Methods: A tissue microarray including 84 specimens from primary oropharyngeal SCC cases has been constructed. Protein expression levels for c-Met were analyzed using both conventional immunohistochemistry (IHC) and a novel system of in-situ protein expression analysis (AQUA). In this system, an anti-cytokeratin antibody conjugated to Cy3 is used to define pixels as tumor within each tissue spot. The intensity of c-Met expression is then measured using Cy5 conjugated antibody with signal amplification. Results: Tumors were stage II (11), III (22), and IV (51). No significant correlations were found for c-Met using traditional IHC. These results contrast with those obtained by AQUA analysis; a significant correlation was found between c-Met and Cox-2 expression levels (p<0.001, Spearman's rho=0.417). The top quartile of c-met expression was also associated with increased local recurrence (p=0.003), and higher all-cause and disease-specific mortality (p=0.0015 and 0.0004). Conclusion: We demonstrated the power of AQUA quantitative protein expression analysis over traditional methods in the identification of molecular prognostic markers in oropharyngeal SCC. We found that patients with the highest levels of c-Met expression have increased mortality and increased local recurrence rates. We also demonstrated a correlation between c-Met and Cox-2 expression, strengthening the evidence for a relationship between these two pathways. No significant financial relationships to disclose.

Tissue microarray

10.1200/jco.2004.22.14_suppl.5517

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Correlates and Determinants of Nuclear Epidermal Growth Factor Receptor Content in an Oropharyngeal Cancer Tissue Microarray

Cancer Epidemiology Biomarkers & Prevention (2008)

Amanda Psyrri Brian L. Egleston Eirini Pectasides Paul Weinberger Ziwei Yu

We have previously reported nuclear localization of epidermal growth factor receptor (EGFR) protein in oropharyngeal cancer tissue. Nuclear EGFR levels were inversely correlated with survival and response to radiotherapy. Here, we sought to identify the determinants and correlates of nuclear EGFR content.We analyzed an oropharyngeal cancer tissue microarray for the expression of the key molecules of the EGFR signaling cascade using an automated image analysis technique (AQUA) scored on a scale of 0 to 255, which permits protein quantitation and subcellular localization. Patients with oropharyngeal squamous cell cancer treated with radiotherapy or surgery and radiotherapy were eligible. Data were analyzed using Spearman correlations and multiple linear regression with robust SEs.Of the 95 tumors included in this study, 72 (75%) had sufficient tissue for analysis of nuclear EGFR. Nuclear EGFR levels were associated with membranous/cytoplasmic EGFR levels (rho = 0.82, P < 0.001), nuclear extracellular signal-regulated kinase-2 (rho = 0.30, P = 0.01), and nuclear proliferating cell nuclear antigen (PCNA; rho = 0.36, P = 0.003). Nuclear phosphorylated-Akt, cyclin D1, phosphatase and tensin homolog (mutated in multiple cancers 1) (PTEN), p53, and proliferation marker Ki-67 levels did not correlate with nuclear EGFR level. In multivariable analysis, only PCNA retained its significant association (P = 0.01).These results are consistent with preclinical data showing that EGFR may function as a tyrosine kinase in the nucleus, phosphorylating and stabilizing PCNA. The nuclear activity of EGFR may constitute a novel therapeutic target.

Tissue microarray

Tensin

10.1158/1055-9965.epi-07-2684

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Citations (40)

Quantitative expression and the predictive value of the components of the retinoblastoma pathway in oropharyngeal squamous cell cancer

Shandong Daxue er-bi-hou-yan xuebao (2006)

Ziwei Yu

Objective: To determine the association between the components of Rb pathway and clinical outcome and prognosis of oropharyngeal carcinoma.Methods: Fluorescent immunohistochemistry for pRb,p16 and cyclin D1 was carried out on a tissue microarray of 95 oropharyngeal cancer cases with long-term patient follow-up data.For evaluation of protein expression,an automated in-situ quantitative measurement of protein analysis(AQUA) was used.Results: Protein levels of pRb and cyclin D1 were significantly associated with local recurrence,disease-free survival and overall survival.In multivariate Cox regression,high nuclear cyclin D1 and high nuclear Rb expression were proved to be independent predictor for local recurrence,disease-free survival and overall survival at 5 years.Conclusion: Our results indicate that the Rb pathway plays a vitally important role for the clinical outcome in oropharyngeal squamous cell carcinoma.Thus,the components of Rb pathway may be potential targets for molecular intervention in patients with oropharyngeal squamous cell carcinoma.

Tissue microarray

Retinoblastoma protein

Retinoblastoma

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Local and Global Point Cloud Reconstruction for 3D Hand Pose Estimation

arXiv (Cornell University) (2021)

Ziwei Yu Linlin Yang Shicheng Chen Angela Yao

This paper addresses the 3D point cloud reconstruction and 3D pose estimation of the human hand from a single RGB image. To that end, we present a novel pipeline for local and global point cloud reconstruction using a 3D hand template while learning a latent representation for pose estimation. To demonstrate our method, we introduce a new multi-view hand posture dataset to obtain complete 3D point clouds of the hand in the real world. Experiments on our newly proposed dataset and four public benchmarks demonstrate the model's strengths. Our method outperforms competitors in 3D pose estimation while reconstructing realistic-looking complete 3D hand point clouds.

Representation

3D Reconstruction

RGB color model

10.48550/arxiv.2112.06389

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Research on the Classification of Concrete Sulfate Erosion Types in Tumushuke Area, Xinjiang

Buildings (2024)

Yuwei Ma Xuemei Jiang Junfeng Li Gang Li Wei Huang

Tumushuke, a significant node of “the China–Pakistan Economic Corridor” and “the Silk Road Economic Belt”, is strategically located in the southern region of Xinjiang. Due to the widespread distribution of its salty soils, concrete construction safety is significantly compromised. The construction of this project used sulfate-resistant cement, which was costly to construct. Six groups with varying sulfate immersion concentrations were set up to perform sulfate erosion tests and sulfate freeze–thaw coupling tests, respectively, based on the survey of the distribution of sulfate concentration in the area. The Tumushuke area’s concrete erosion kinds were classified using a microanalysis of the degraded concrete. The findings indicate that the concrete primarily exhibits gypsum-type erosion when the sulfate concentration is greater than 20,000 mg/kg, ettringite–gypsum-type erosion when the sulfate concentration is between 15,000 and 20,000 mg/kg, and ettringite-gypsum-type erosion when the sulfate concentration is less than 15,000 mg/kg. The erosion product, carbon–sulfur silica-calcite, also occurs under sulfate freeze–thaw coupling. In the Tumushuke area, ettringite-type erosion damage is primarily found in low-sulfate areas in the southwest and a small portion of the northeast. In contrast, higher-sulfate areas in the central northward area are primarily affected by ettringite–gypsum and gypsum-type erosion damage. The results of this study can provide a basis for adopting different anti-sulfate erosion measures for engineering construction in different regions.

10.3390/buildings14030729

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Citations (2)

Association between knee alignment, articular cartilage degradation, and subchondral trabecular bone remodeling in patients with knee osteoarthritis

Osteoarthritis and Cartilage (2020)

Ziwei Yu Xuequan Han Mengning Yan

Subchondral bone

Bone remodeling

10.1016/j.joca.2020.02.342

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Quantitative Determination of Nuclear and Cytoplasmic Epidermal Growth Factor Receptor Expression in Oropharyngeal Squamous Cell Cancer by Using Automated Quantitative Analysis

Clinical Cancer Research (2005)

Amanda Psyrri Ziwei Yu Paul Weinberger Clarence T. Sasaki Bruce G. Haffty

Abstract Background: Several lines of evidence support the epidermal growth factor receptor (EGFR) as a molecular target for therapy in head and neck squamous cell carcinomas (HNSCC). Determination of tumor EGFR levels by conventional immunohistochemistry has not always predicted antitumor efficacy. Quantitative assays may provide more accurate assessment of the level of EGFR receptor in the tumor, which may thus provide more reliable prognostic and predictive information. We studied the prognostic value of quantitative assessment of EGFR in oropharyngeal squamous cell cancers treated with radiotherapy. Experimental Design: We studied EGFR protein expression on a tissue microarray composed of 95 oropharyngeal cancer cases using an in situ molecular-based method of quantitative assessment of protein expression (AQUA) and correlated those with clinical and pathologic data. Automated, quantitative analysis uses cytokeratin to define pixels as cancer (tumor mask) within the array spot and measures intensity of EGFR expression using a Cy5-conjugated antibody within the mask. A continuous index score is generated, which is directly proportional to the number of molecules per unit area, and cases were defined as high expressing if they were above the median expression level. Results: The mean follow-up time for survivors was 44.9 months, and for the entire cohort was 34.8 months. Patients with high tumor EGFR expression levels had a local recurrence rate of 58% compared with 17% for patients with low EGFR tumor expression (P < 0.01). Similarly, patients with high nuclear EGFR expression had a local recurrence rate of 54% compared with 21% for patients with low EGFR nuclear expression (P < 0.05). Additionally, patients with high tumor and nuclear EGFR levels had inferior disease-free survival compared with low expressors (19% versus 43% and 19% versus 45%, respectively. P < 0.05 for each). In multivariate analysis adjusting for well-characterized prognostic variables, high tumor and nuclear EGFR expression levels retained their prognostic significance. Conclusion: The AQUA system provides a continuous measurement of EGFR on paraffin-embedded tissue and was able to reveal the association between EGFR expression and outcome expected from the biological role of EGFR. In the future, EGFR AQUA score may be useful in predicting response to EGFR-targeted therapies.

Tissue microarray

10.1158/1078-0432.ccr-05-0420

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Citations (263)

RNA methyltransferase NSUN2 promotes hypopharyngeal squamous cell carcinoma proliferation and migration by enhancing TEAD1 expression in an m5C-dependent manner

Experimental Cell Research (2021)

Lixiao Chen Jian Ding Baoxin Wang Xinwei Chen Xinjiang Ying

10.1016/j.yexcr.2021.112664

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Citations (26)

Overcoming the Trade-off Between Accuracy and Plausibility in 3D Hand Shape Reconstruction

arXiv (Cornell University) (2023)

Ziwei Yu Chen Li Linlin Yang Xiaoxu Zheng Michael Bi Mi

Direct mesh fitting for 3D hand shape reconstruction is highly accurate. However, the reconstructed meshes are prone to artifacts and do not appear as plausible hand shapes. Conversely, parametric models like MANO ensure plausible hand shapes but are not as accurate as the non-parametric methods. In this work, we introduce a novel weakly-supervised hand shape estimation framework that integrates non-parametric mesh fitting with MANO model in an end-to-end fashion. Our joint model overcomes the tradeoff in accuracy and plausibility to yield well-aligned and high-quality 3D meshes, especially in challenging two-hand and hand-object interaction scenarios.

Parametric model

3D Reconstruction

3d model

Parametric equation

10.48550/arxiv.2305.00646

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