Yoshiyuki Maeda

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Characterization of new microsatellite loci in Pieris amamioshimensis (Ericaceae), a species nearly extinct in the wild

Plant Species Biology (2017)

Yuki Mitsui Junichi Nagasawa Yoshiyuki Maeda Hiroaki Setoguchi

Abstract A set of 11 polymorphic microsatellite markers has been developed and characterized for the critically endangered species Pieris amamioshimensis . Fifty‐nine individuals of an ex‐situ population were used to identify these markers. The total number of alleles for each locus ranged from 3 to 9, with an average of 5.4. The expected heterozygosities ( H S ) and observed heterozygosities ( H O ) ranged from 0.47 to 0.77 and 0.22 to 0.88, respectively. In total, four loci exhibited significant deviations from Hardy–Weinberg equilibrium: two loci showed significant heterozygosity excess and the other two loci showed significant heterozygosity deficit. The polymorphism information content (0.43 ≤ PIC ≤ 0.73), the probability of exclusions (PE 1 = 0.9565, PE 2 = 0.9969 and PE 3 = 0.9999) and probabilities for identity (PI = 3.78 × 10 −9 and PI‐Sib = 2.35 × 10 −4 ) suggest that these markers are useful for estimating not only genetic diversity but also parentage, for the ex‐situ conservation management of populations.

Ex situ conservation

Linkage Disequilibrium

10.1111/1442-1984.12193

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Fate of Chlorophyll a during its Ethylene-Enhanced Chlorophyll Degradation in Citrus unshiu Fruits.

Journal of the Japanese Society for Horticultural Science (1994)

Atsushi Yanagisako Yoshiyuki Maeda Keishi Shimokawa

Two enzymatic metabolites from chlorophyll a were detected in crude enzyme extracts preparated from ethylene-treated fruits of Citrus unshiu. They were identified as pheophorbide a and pyropheophorbide a using high performance thin layer and liquid chromatographic techniques and fluoresence spectroscopy.Sodium ascorbate enhanced conversion of chlorophyll a to pheophorbide pigments, whereas p-chloromercuribenzoate strongly inhibited the formation of pyropheophorbide a. These results suggest that the crude enzyme extracts contain the two enzymes, 'Mg-de-chelatase' and 'Decarbomethoxylase'

Pheophorbide A

Citrus unshiu

Pheophytin

Degradation

10.2503/jjshs.63.183

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Effects of betacyanin on salt tolerance in Swiss chard (Beta vulgaris var. cicla)

Ningen to kankyo (2020)

Yoshiyuki Maeda Hiroyuki Oshima Yohhei SHIBUYA

本研究は抗酸化能をもつベタシアニンを含有するフダンソウの耐塩反応をナトリウム（Na）イオンの侵入抑制と酸化ストレス軽減の観点からホウレンソウと比較検討した。その結果，耐塩性はホウレンソウよりもフダンソウで高かった。ホウレンソウのNa含有量はNa処理により根部，地上部ともに増加した。細胞膜ATPase活性，グリシンベタイン含有量から検討した根部のNa侵入抑制能力はフダンソウよりも小さかった。一方，フダンソウのNa処理による体内Na含有量の増加パターンはホウレンソウと大きく異なった。すなわち，生育が維持されたNa処理濃度（生育維持濃度）以内では処理濃度の増加とともに地上部のNa含有量は増加した。しかし，それ以上の処理濃度では増加しなかった。逆に，根部のNa含有量はNa処理により増加したものの，生育維持濃度以内では変化せず，その濃度を超えると急激に増加した。過酸化水素含有量とSOD活性は両植物ともにNa処理によって高まったが，ホウレンソウのこれら値は生育維持濃度で，フダンソウの値は生育阻害濃度でそれぞれ著しく増加した。ホウレンソウのカタラーゼ活性はNa処理によって，フダンソウの活性は生長阻害濃度で著しく増加した。一方，ベタシアニン含有量はNa処理の有無や濃度に関わらず変化せず，フダンソウの地上部に一定量存在した。

BETA (programming language)

10.5793/kankyo.46.2_3

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Salt tolerance in reed canarygrass (Phalaris arundinacea L.) grown on soil perfused with cattle urine

Springer eBooks (1997)

Yoshiyuki Maeda Hiroshi Takenaga

Phalaris arundinacea

10.1007/978-94-009-0047-9_178

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Citations (1)

Inhibition of Allylisothiocyanate on Ethylene-Enhanced Chlorophyll Degradation in Citrus unshiu Fruit

Journal of the Japanese Society for Horticultural Science (1994)

Keishi Shimokawal Hidehiro Hashimoto Yoshiyuki Maeda Yoshinori Uchida Yasushi Shibasaki

Effects of allylisothiocyanate (an inhibitor of ethylene action) on ethylene-enhancement of chlorophyllase in satsuma mandarin (Citrus unshiu) fruit were investigated. The aim is to accumulate knowledge of action mechanism of ethylene. The isothiocyanate inhibited ethylene-enhancement of chlorophyllase activity in vivo. A correlation (r=0.9580, significant at 0.1% level) exists between chlorophyllase activity and Hunter's color index, (L+ b) /2+a, a marker of degreening. Participation of 'chlorophyllide a peroxidase', a candidate enzyme of degreening, was recognized, in independent of ethylene and/or allyl-isothiocyanate treatments. It is conclusive that chlorophyllase is a key enzyme in ethylene-enhanced chlorophyll degradation of satsuma mandarin fruit.

Citrus unshiu

Degradation

10.2503/jjshs.63.195

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Citations (5)

Vespula shidai amamiana (Hymenoptera, Vespidae) : Perennial Colonies and Polygyny in subtropical Japan

Biogeography (2008)

Seiki Yamane Yoshiyuki Maeda

Polygyny

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Comparison of Ca Effect on the Salt Tolerance of Suspension Cells and Intact Plants of Tobacco (Nicotiana tabacumL., cv. Bright Yellow-2)

Soil Science & Plant Nutrition (2005)

Yoshiyuki Maeda Masaaki Yoshiba Toshiaki Tadano

Abstract Salt tolerance and the effect of Ca application on the salt tolerance were compared between suspension culture cells and intact plants of tobacco (Nicotiana tabacum L., cv. Bright Yellow-2). The suspension culture cells and intact plants of tobacco were grown in a culture medium without NaCl and with 100 mM NaCl containing 0.3 or 10 mM CaCl2. At 7 d after the treatment, the dry weight of the cells and intact plants grown in the medium containing 100 mM NaCl with 0.3 mM CaCl2 decreased and the decreasing rate of the weight was much higher in the intact plants. In the medium containing 100 mM NaCl with 10 mM CaCl2, the growth of the cells and intact plants was improved, and the improvement of the growth by Ca application was much more significant in the cultured cells. Sodium content in the intact plants markedly increased with the increase of the NaCl concentration, compared to that in the cells. Calcium application decreased the Na content both in the cells and intact plants and the decreasing rate was higher in the cells than in the intact plants. Thus, it was suggested that the growth inhibition and the increase of the Na content under 100 mM NaCl conditions were more pronounced in intact plants, and that the alleviatory effect of Ca on the growth inhibition was much more conspicuous in the cells. Although the contents of total Ca and pectic acid-binding Ca in the cells and plant roots decreased with the increase of the NaCl concentration, they increased by Ca application. The activity of plasmalemma ATPase of the cells and plant roots decreased in the medium containing 100 mM NaCl with 0.3 mM CaCl2, but increased by Ca application, regardless of the NaCl concentration. In summary, Ca application to the culture medium containing 100 mM NaCl alleviated the growth inhibition, and the difference in the growth inhibition induced by 100 mM NaCl between the suspension culture cells and intact plants was closely related to that in the Na content. It appears that the alleviatory effect of Ca on the growth inhibition might be caused mainly by the enhancement of the Na-excluding function. The high content of pectic acid-binding Ca on the cell wall also contributed moderately to the alleviatory effect of Ca through the decrease of apoplasmic Na translocation. Key words: ATPasecalciumintact plantsalt tolerancetobacco suspension cells

Plant cell

Suspension

10.1111/j.1747-0765.2005.tb00056.x

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Citations (10)

Measurements in a two-dimensional curved jet along a circular arc

Wataru Masuda Yoshiyuki Maeda

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Common reed produces starch granules at the shoot base in response to salt stress

New Phytologist (2007)

Masatake Kanai Kyoko Higuchi Takashi Hagihara Teruko Konishi Tadashi Ishii

Common reed (Phragmites australis) is a well known salt-tolerant plant and it is suggested that reeds recover Na(+) in the xylem sap of the shoot base (basal part of the shoot), store it temporarily in the shoot base, release it into the phloem sap, and then retranslocate it to the roots. To investigate whether Na(+) is retained in the shoot base of reeds, confocal laser scanning microscope (CLSM) observations were conducted using an intracellular Na(+)-specific fluorescent probe. The CLSM observations revealed that reeds produced a large number of the starch granules at the shoot base when salt-stressed, and that the fluorescence indicating the location of intracellular free Na(+) was observed in the same position as the starch granules. The Na content of starch granules was considerably greater than that of the shoot base, whereas the potassium (K) contents of the granules was only slightly greater than that of the shoot base. Reeds produced Na(+)-binding starch granules in the parenchyma cells of the shoot base when salt-stressed; these starch granules may decrease intracellular free Na(+). It is proposed that the site-specific production of Na(+)-binding starch granules constitutes a novel salt tolerance mechanism.

Phragmites

10.1111/j.1469-8137.2007.02188.x

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Citations (90)

Salt tolerance of five temperate grasses at germination and different growth stag

Journal of the Japanese Society of Revegetation Technology (2001)

Yoshiyuki Maeda Hiroko Ukai Hiroshi Takenaga

乾燥および塩類集積地域の緑化材料として5種類の寒地型牧草を設定し, 高塩類条件下における発芽率, 生長量および地上部・根部カチオン含量を調査し, 発芽時および栄養生長期の生育ステージ別の, 牧草種間の耐塩性強弱を比較検討した。供試牧草としてイタリアンライグラス (IRG), メドウフェスク (MF), リードカナリーグラス (RCG), トールフェスク (TF) およびアルファルファ (ALF) を設定した。IRG, TFおよびALFの発芽率は50～150mMNaCl条件下で80%以上の高い値を示した。一方, RCGの値はNaCl濃度の増加とともに直線的に低下し, 供試牧草間で最も低かった。生育ステージの進行とともにいずれの草種も耐塩性は強まった。生育ステージに関わらずTFの耐塩性はもっとも強く, 次いでIRG, RCG, MFの順で, ALFの耐塩性は最も弱かった。また, 発芽時と生育時の耐塩性の評価はALFを除いてほぼ一致した。塩添加により全草種とも地上部および根部のNa含量は高まり, K, MgおよびCa含量は低下し, Na/K比は増加した。しかし, 生育ステージの進行とともに塩添加による植物体中のNa増加率, K, MgおよびCa低下率, およびNa/K比はいずれの草種も低下した。供試牧草間で耐塩性の最も強かったTFのこれらの値は全生育ステージで最も低かった。以上の結果から, 草種間の耐塩性強弱は塩溶液中の発芽率で判断することが可能であった。また, 塩処理によって生じる植物体内カチオン動態を制御する能力が高い草種ほど耐塩性は強く, またこの制御能力は生育とともに高まることが明らかになった。

10.7211/jjsrt.27.499

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Citations (1)