Wang Yong-qi

Tianjin University of Science and Technology

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Baomin Feng

Dalian University

Liying Shi

Dalian University

Ling Tang

First Affiliated Hospital of Anhui Medical University

Ting Xia

Tianjin University of Science and Technology

Yuewen Fu

Zhengzhou University

Wenhua Liu

Harbin Medical University

Xing‐Guo Yan

Dalian University

Xiaofang Cao

Karolinska Institutet

Feng Baomin

Dalian University

Xiaoli Bai

Tasly Holding Group (China)

Cooperative Institutions

Chinese Academy of Medical Sciences & Peking Union Medical College

Peking University

Dalian University

Institute of Hematology & Blood Diseases Hospital

Zhengzhou University

Huazhong University of Science and Technology

Chinese Academy of Sciences

Central South University

Sun Yat-sen University

Capital Medical University

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Optimization of Technology by Orthogonal Test for Extracting Total Flavonoids from Camellia semiserrata Chi. Seeds

Lishizhen Medicine and Materia Medica Research (2008)

Wang Yong-qi

Objective To optimize the technology for extracting total flavonoids from Camellia semiserrata Chi.seeds.Methods The seeds were extracted using ethanol-aqueous,and the extract rate of total flavonoids were studied by orthogonal test,inspecting solvent concentration of extraction,the effects of time of extraction,temperature of extraction,number of times,solvent times of extraction.Results The optimum extracting technology was as follows: with 20 times 70% ethanol-aqueous,extracting 2 times at 80℃ for 2.5 h once.By this technology the extract rate of total flavonoids was 3.42% and the content of total flavonoids was 18.55%.Conclusion This method can be applied to the extraction of total flavonoids in Camellia semiserrata Chi.seeds.The extracting solvent times shows objective effects.

Camellia

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Study the 5α-reductase Inhibitory Activity of Urtica fissa E. Pritz's Extract

Lishizhen Medicine and Materia Medica Research (2009)

Wang Yong-qi

Objective To study the 5α-reductase inhibitory activity of the aqueous constituents in Urtica fissa E.Pritz's ectract.MethodsThe 5α-reductase inhibitor's model in vitro included a mini-reactive system which was composed of 5α-reductase extracted from the prostates of male rats,testosterone(as a substrate) and NADPH(as a hydrogen supplier).The concentration of NADPH was measured by the fluorescence examinination.The specificity of 5α-reductase was judged with finasteride as a positive control.ResultsThe absorbance of fluorescence in the reactive system which was added with the extract of Urtica fissa E.Pritz was remarkably higher than the reference group.Conclusion The Urtica fissa E.Pritz's aqueous extract can inhibit the activity of the 5α-redutase in vitro.

Urtica dioica

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Studies on the Constituents of Scutellaria Species-2-High Performance Liquid Chromatography of Glucuronyflavonoids and Seasonal Variation of Their Contents in Scutellaria ikonnikovii Juz.(速報)

Shoyakugaku Zasshi (1990)

Wang Yong-qi Keiichi Matsuzaki Kunio Takahashi Toru Okuyama

Scutellaria

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Electrification Prostatectomy of Single Lobe for Treatment of Serious Benign Prostatic Hyperplasia (Report of 39 cases)

Journal of Clinical Medical officer (2000)

Wang Yong-qi

Objective To observe curative effect and method of transurethral electrification of prostatectomy of single lobe for treatment of serious benign prostatic hyperplasia (BPH) Method 39 cases of BPH were treated by using transurethral electrification of prostatetomy of single lobe Result The mean operative time, intraoperative bleeding and weight of prostate tissue resected were 43 minutes, 50 ml and 37 g, respectively No patients experienced TUR syndrome and serious bleeding 31 patients were followed up for 1～24 months IPSS decreased from 34.3 to 8.7 and MFR increased from 5.6 ml/s to 17.4 ml/s Conclusion Transurethral electrification of prostatetomy of single lobe was an operative method with less bleeding and shorter operative time for serious BPH

Lobe

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Determination of the Total Sinapinoids Content in the seeds Sinapis alba. L and Brassica Juncea

Journal of Dalian University (2009)

Wang Yong-qi

The total sinapinoids was extracted with methanol,ethanol and water.Colorimetry method was used to determine the total sinapinoids in the article.It was showed from the results that,the maximum total sinapinoids content in the seeds of Sinapis alba.L.is 2.75% and the minimum content is 0.52%,and that those in the Brassica juncea are 1.29% and 0.70% respectively.The content of total sinapinoids is higher by extraction with EtOH and it also showed great variation for different habitats.

Sinapis

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Isolation and identification of chemical constituents of root of Girardinia suborbiculata C.J.Chen subsp.triloba

Shenyang Yaoke Daxue xuebao (2011)

Wang Yong-qi

Objective To study the chemical constituents of traditional Chinese medicine,the root of Girardinia suborbiculata C.J.Chen subsp.triloba.Methods Chemical constituents were isolated by various chromatographic methods and identified by physiochemical and spectral analysis.Results Ten compounds were isolated and identified as aurantiamide acetate(1),oleanolic acid(2),2α,3α,19α-trihydroxyurs-12-en-28-oic(3),scopoletin(4),3-methoxy-4-hydroxybenzoic acid(5),p-hydroxybenzoic acid(6),β-sitosterol(7),daucosterol(8),5-hydroxymethyl furaldehyde(9)and mannose(10).Conclusions Compounds 16,810 are isolated from Girardinia species for the first time.

scopoletin

Daucosterol

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Characterization of the bioactive compounds with efficacy against epilepsy from the herb pairs Polygala tenuifolia - Zizyphus jujuba by modulating CHRNA4/CaMKII signaling pathway: LC-MS/MS combined with network pharmacology analysis and experimental evidence

Research Square (Research Square) (2023)

Meng Nian Zefeng Zhao Wang Yong-qi Jingxuan Chen Mingcheng Qian

Abstract Background Epilepsy is a typical nervous system disorders identified by the spontaneous recurrence of seizures which injure periods of electroencephalographic activity and behavior. Traditional Chinese Medicine (TCM) herb pairs Polygala tenuifolia and Zizyphus jujuba have been used in treatment of epilepsy in China, while the mechanism of action still remains unclear. This article aims to disclose the substances and potential mechanisms of the anti-epilepsy activity of P. tenuifolia and Z. jujuba extract ( PZE ) using LC-MS/MS, network pharmacology, ethology and molecular biology methods. Methods With the help of the self-built components database, identification of the chemical parameters of PZE was possessed through LC-MS/MS method, and the “ingredient-target-pathway” network of PZE was established through online databeses. Molecular docking was performed using Discovery Studio Visualizer. In the setting of the epilepsy model, pentylenetetrazol (PTZ, 10 mg/kg) was administered intraperitoneally injected for a period of 21 days. Mice were assessed for anxiety-like behavior by Elevated plus maze test, open field test, forced swimming test and tail suspension test. HE staining, western blotting, and immunofluorescence staining were used to detect morphological changes and signal pathway. Results Through network analysis, 37 active ingredients were obtained from PZE , SLC6A4, CHRNA4 and MAOA and were found to play a major role in the PPI network. GO and KEGG analyses that display their anti-epilepsy activity. The"Ingredient-target-pathway"network diagram consists of 99 targets, 24 kinds of constituents, and 20 signaling pathways. The values of M15 and M17 show the largest degree. Molecular docking analysis shows the key components screened by network pharmacology have a good interaction with the predicted targets. Animal experiments results showed that: 1) PZE effectively lengthened the latent time of PTZ-induced epilepsy in mice model. 2) PTZ-induced depression-like behavior was strikingly ameliorated by PZE . 3) Hippocampal neurons are significantly shielded by PZE . 4) PZE was shown to play a key role in modulating the CHRNA4/CaMK II signaling pathway in to show anti-epilepsy potency. Conclusion This study has successfully identified constituents of PZE through LC-MS/MS methods and predicted the potential targets and CHRNA4/CaMK II as potential signaling pathways of anti-epilepsy effects for PZE , which was proved by animal experiments. The results of this paper are conducive to the systematic elucidating of its mechanism of action and the development of TCM-based anti-epilepsy agents.

KEGG

10.21203/rs.3.rs-2698704/v1

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Development of a loop-mediated isothermal amplification assay for detection of forest musk deer Arcanobacterium pyogenes.

JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY (2014)

Jie Tang Liu ErLong Lu Li Feiran Li Wang Yong-qi

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Risk factors of acute renal failure associated with liver transplantation during operation

Journal of Lanzhou University (2010)

Wang Yong-qi Yatao Liu

Objective To study the risk factors of renal failure associated with liver transplantation (RFALT) during orthotopic liver transplantation(OLT).Methods 53 patients without renal disfunction undergoing OLT were studied.Blood samples were drawn for determination of creatinine and blood urea nitrogen during operation and after operation.Then they were divided into two groups according the standard of Rimola et al:patients with RFALT and patients without RFALT.Nine variables were recorded and analyzed with univarite analysis and stepwise logistic regression to identify the risk factors.Results 14 patients were diagnosed as RFALT a week after operation.Univarite analysis indicated that five variables,including duration of anhepatic phase,volume of intraoperative blood-loss,volume of intraoperation blood transfusion,low blood pressure and the concentration of norepinephrine had significant difference between two groups. The stepwise logistic regression analysis for 5 variables demonstrated that duration of anhepatic phase(≥60 min) was the independent risk factor of acute renal failure during OLT.Conclusion Duration of anhepatic phase≥60 min was the independent risk factor of acute renal failure during OLT.Shorten the duration of anhepatic phase,decrease the volume of intraoperation blood transfusion and increase infusion of renal in order to decrease RFALT.

Blood urea nitrogen

Stepwise regression

Orthotopic liver transplantation

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Effect of active part extracted from Tadehagi trquetrum(Linn.) Ohashi on type I allergy induced by IgE

Central South Pharmacy (2011)

Wang Yong-qi

Objective To determine the effect of active part extracted from Tadehagi trquetrum(Lim.) Ohashi on type Ⅰ allergy induced by IgE.Methods Tadehagi trquetrum(Linn.) Ohashi was extracted by 50% acetone.The 50% acetone extractions were again extracted by EtOAc and isolated by D101 chromatography column.We got 50% acetone extraction(1),EtOAc extraction(2),H2O layer(3),residue(4),and enriched extract by macroporous resin D101(5).Rats were sensitized with the mixture of OVA and Al(OH)3 to establish the experimental rat model of type Ⅰ allergy.The rats were divided into 13 groups ramdomly,namely a normal group,a model group,a menglusitena group,and sample group 1,2,3,4,5 at 100 mg·kg-1 or 400 mg·kg-1 respectively.IgE,LT and His contents in the serum of sensitized rats were measured with ELISA.The inflammatory area in the pulmonary tissues through pathological examination.Results Group 1,2,3 decreased the contents of IgE,LT and His in the serum.The active sequence was 123.Group 1 reduced the quantities of EOS in whole blood and BALF;Group 1 and 2 reduced the inflammatory areas in the pulmonary tissue,and the active sequence was 12.Conclusion Group 1,2,and 3 have obvious anti-alergy effect on type Ⅰ allergy and the active sequence is 123.

Residue (chemistry)

Normal group

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