Jianhua He

Wuhan University

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Feng Yu

Shanghai Institute of Applied Physics

Huan Zhou

Jinan University

Qisheng Wang

Chinese Academy of Sciences

Bo Sun

Shanghai Advanced Research Institute

Chunyan Xu

Xinyu University

Lin Tang

Xi'an Technological University

Zhijun Wang

Chinese Academy of Sciences

Jian Li

Chinese Academy of Sciences

Weiwu Wang

Nanjing Agricultural University

Minjun Li

Shanghai Advanced Research Institute

Cooperative Institutions

Chinese Academy of Sciences

248

University of Chinese Academy of Sciences

123

Shanghai Institute of Applied Physics

Shanghai Jiao Tong University

Sichuan University

Shanghai Advanced Research Institute

Wuhan University

Zhejiang University

Tsinghua University

Shanghai Institute of Materia Medica

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New design for multi-crystal data collection at SSRF

Nuclear Science and Techniques (2018)

Bing Li Sheng Huang Pan Qiangyan Min-Jun Li Huan Zhou

Kapton

Protein crystallization

Goniometer

Data set

Crystal (programming language)

10.1007/s41365-018-0357-5

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Citations (4)

Molecular determinants of human neutralizing antibodies isolated from a patient infected with Zika virus

Science Translational Medicine (2016)

Qihui Wang Huabing Yang Xiaoqing Liu Lianpan Dai Tong Ma

Zika virus–specific antibodies isolated from a single infected patient show postexposure protection in mice and reveal targets for therapy.

Zika Virus

10.1126/scitranslmed.aai8336

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Citations (211)

Single pulse data collection with an X-ray chopper at in situ room temperature Laue crystallography beamline BL03HB

Nuclear Instruments and Methods in Physics Research Section A Accelerators Spectrometers Detectors and Associated Equipment (2024)

Yu Li Zhijun Wang Cheng Pan Weizhe Zhang Bo Sun

Chopper

10.1016/j.nima.2024.169891

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Citations (0)

Structural Insights into the Mechanism for Recognizing Substrate of the Cytochrome P450 Enzyme TxtE

PLoS ONE (2013)

Feng Yu Minjun Li Chunyan Xu Zhijun Wang Huan Zhou

Thaxtomins, a family of phytotoxins produced by Streptomyces spp., can cause dramatic plant cell hypertrophy and seedling stunting. Thaxtomin A is the dominant form from Streptomyces scabies and has demonstrated herbicidal action. TxtE, a cytochrome P450 enzyme from Streptomyces scabies 87.22, catalyzes direct nitration of the indolyl moiety of L-tryptophan to L-4-nitrotryptophan using nitric oxide, dioxygen and NADPH. The crystal structure of TxtE was determined at 2.1 Å resolution and described in this work. A clearly defined substrate access channel is observed and can be classified as channel 2a, which is common in bacteria cytochrome P450 enzymes. A continuous hydrogen bond chain from the active site to the external solvent is observed. Compared with other cytochrome P450 enzymes, TxtE shows a unique proton transfer pathway which crosses the helix I distortion. Polar contacts of Arg59, Tyr89, Asn293, Thr296, and Glu394 with L-tryptophan are seen using molecular docking analysis, which are potentially important for substrate recognition and binding. After mutating Arg59, Asn293, Thr296 or Glu394 to leucine, the substrate binding ability of TxtE was lost or decreased significantly. Based on the docking and mutation results, a possible mechanism for substrate recognition and binding is proposed.

Docking (animal)

10.1371/journal.pone.0081526

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Citations (26)

Microfluidic rotating-target device capable of three-degrees-of-freedom motion for efficient in situ serial synchrotron crystallography

Journal of Synchrotron Radiation (2023)

Feng‐Zhu Zhao Zhijun Wang Qingjie Xiao Li Yu Bo Sun

There is an increasing demand for simple and efficient sample delivery technology to match the rapid development of serial crystallography and its wide application in analyzing the structural dynamics of biological macromolecules. Here, a microfluidic rotating-target device is presented, capable of three-degrees-of-freedom motion, including two rotational degrees of freedom and one translational degree of freedom, for sample delivery. Lysozyme crystals were used as a test model with this device to collect serial synchrotron crystallography data and the device was found to be convenient and useful. This device enables in situ diffraction from crystals in a microfluidic channel without the need for crystal harvesting. The circular motion ensures that the delivery speed can be adjusted over a wide range, showing its good compatibility with different light sources. Moreover, the three-degrees-of-freedom motion guarantees the full utilization of crystals. Hence, sample consumption is greatly reduced, and only 0.1 mg of protein is consumed in collecting a complete dataset.

10.1107/s1600577523000462

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Citations (3)

Neuron-restrictive silencer factor in periaqueductal gray contributes to remifentanil-induced postoperative hyperalgesia via repression of the mu-opioid receptor

Journal of the Neurological Sciences (2015)

Cui’e Lu Linyu Shi Juan Zhang Mingjian Kong Yue Liu

Allodynia

Periaqueductal gray

μ-opioid receptor

10.1016/j.jns.2015.03.018

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Citations (16)

469 (PB457): Nanoparticle-induced virus immune tolerance during oncolytic therapy of gliomas

European Journal of Cancer (2024)

Dong Hyuk Shin Bülent Özpolat Alison Gillard Marc Garcia Moure Hong Jiang

10.1016/j.ejca.2024.114976

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Citations (0)

Identification of a new series of potent diphenol HSP90 inhibitors by fragment merging and structure-based optimization

Bioorganic & Medicinal Chemistry Letters (2014)

Jing Ren Jian Li Yueqin Wang Wuyan Chen Aijun Shen

Hsp90 inhibitor

Target protein

Chaperone (clinical)

10.1016/j.bmcl.2014.03.100

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Citations (19)

A novel sample delivery system based on circular motion for in situ serial synchrotron crystallography

Lab on a Chip (2020)

Feng‐Zhu Zhao Bo Sun Yu Li Qingjie Xiao Zhijun Wang

A microfluidic rotating-target sample delivery device based on circular motion for in situ serial crystallography is presented, which combines the advantages of circular motion, microfluidic technology and in situ diffraction.

Circular motion

10.1039/d0lc00443j

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Citations (15)

Status of the crystallography beamlines at SSRF

The European Physical Journal Plus (2015)

Jianhua He Xingyu Gao

Undulator

10.1140/epjp/i2015-15032-6

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Citations (5)