Chang Xue

Wenzhou Medical University

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Zai‐Sheng Wu

Fuzhou University

Zhifa Shen

Wenzhou Medical University

Huo Xu

Minjiang University

Chan Li

Southern Medical University

Qiufeng Song

Wenzhou Medical University

Guohui Xue

Jiujiang First People's Hospital

Danhong Wang

Athinoula A. Martinos Center for Biomedical Imaging

Guoqiao Huang

Wenzhou Medical University

Jing Zhang

Jiangxi University of Traditional Chinese Medicine

Xin Yu

Case Western Reserve University

Cooperative Institutions

Wenzhou Medical University

Chinese Academy of Sciences

Fuzhou University

Nankai University

Huazhong University of Science and Technology

Chinese Academy of Medical Sciences & Peking Union Medical College

Sichuan University

Jilin University

University of Chinese Academy of Sciences

Sun Yat-sen University

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Y-Shaped Backbone-Rigidified Triangular DNA Scaffold-Directed Stepwise Movement of a DNAzyme Walker for Sensitive MicroRNA Imaging within Living Cells

Analytical Chemistry (2019)

Chang Xue Songbai Zhang Congcong Li Xin Yu Changhe Ouyang

DNA as a programmable molecule shows great potential in a wide variety of applications, with the dynamic DNA nanodevices such as DNA motors and walkers holding the most promise in controlled functions for biosensing and nanomedicine. However, a motor or walker that consists of DNA exclusively has not been shown to function within cells because of its susceptibility to endogenous nuclease-mediated degradation. In this contribution, we demonstrate a Y-shaped backbone-rigidified triangular DNA scaffold (YTDS)-directed DNAzyme walker that functions inside living cells to detect microRNAs (miRNAs) with high sensitivity. A novel Y-shaped backbone offers access to geometrically well-defined configurations and increases the rigidity of DNA assemblies, providing a unique, circular, and rigid DNA track within living cells without non-nucleic acid auxiliary materials and enabling the stepwise movement of DNAzyme in an inchworm fashion. This strategy is extended to the construction of larger rigid planar geometric polygon-based DNA walkers, demonstrating unprecedented opportunities to build dynamic DNA nanostructures with precise geometry and versatile functionality.

Deoxyribozyme

DNA nanotechnology

Nuclease

A-DNA

10.1021/acs.analchem.9b03784

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Citations (67)

Nanowire Stacked Bimetallic Metal-Organic Frameworks for Asymmetric Supercapacitor

SSRN Electronic Journal (2022)

Jianli Wang Jing Liang Yuchen Lin Kejin Shao Chang Xue

Metal-organic frameworks (MOFs) have broad application prospects in the field of supercapacitors due to their unique construction and tunable pore size. However, maintaining high energy density under high power density is still a difficult problem to limit the application of supercapacitors. Herein, a simple, mild, and one-step hydrothermal process was used to fabricate Ni-MOF crystals, which was a template for preparing flower-like NiCo-MOF-2 by in-situ etching methods. Interestingly, the "petals" of NiCo-MOF-2 are formed by stacking multiple nanowires, which can enhance richer active sites to promote charge transfer owing to the larger contact area between the electrolyte and the electrode material. Consequently, the NiCo-MOF-2 exhibits a larger specific capacitance of 781 F g -1 at a current density of 0.5 A g -1 and the assembled asymmetric supercapacitor (NiCo-MOF-2//AC) with a stack energy density of 45.7 Wh kg -1 at a power density of 450.6 W kg -1 .

Bimetallic strip

10.2139/ssrn.4086198

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Endogenously Activated and Self‐Reinforced DNA Lipid Nanodevice for Spatial‐Specific and High‐Contrast Imaging of MicroRNA in Cells and Animals

Advanced Functional Materials (2024)

Chan Li Haiyan Jia Wangxin Zhao Rong Wu Xiaoyu Zhou

Abstract Tumor‐specific microRNA (miRNA) imaging strategies are critical for investigating mechanisms associated with cancer progression; however, nonspecific signal leakage and false‐positive signaling limit their selectivity and efficiency. In this paper, an endogenously activated and self‐reinforced DNA lipid nanodevice (LND) for spatial‐specific and high‐contrast imaging of miRNAs in living cells and animals is presented. The LND is functionalized on cholesterol‐based lipid micelles (CLMs) containing smart response and self‐fueling DNA components that display redox‐activatable and autocatalytic miRNA probing activities, respectively. The LNDs are initially silenced and selectively activated using glutathione, an endogenous microenvironmental factor overexpressed in tumor cells, to prevent nonspecific signal leakage. Subsequently, an autocatalytic DNA circuit is used for tumor‐specific and high‐contrast miRNA imaging. It is demonstrated that robust CLM nanoparticles can be easily assembled from a cholesterol‐conjugated G‐rich DNA sequence by stirring in a buffered solution, enabling the LND to enter cells naturally. In addition, in vitro and in vivo data illustrate that the enhanced biostability of DNA components on the surface of LNDs can be realized for minimizing false‐positive signals, making the system ideal for miRNA imaging in living mice. This smart LND technique has great potential for precise biomedical imaging and clinical diagnosis.

Nanodevice

10.1002/adfm.202405780

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Citations (2)

Self-Interpenetrated Water-Stable Microporous Metal–Organic Framework toward Storage and Purification of Light Hydrocarbons

Inorganic Chemistry (2021)

Yang Qiao Chang Xue Jin‐Yu Zheng Mao Yi Ze Chang

Storage and purification of light hydrocarbons are very meaningful for their high-purity requirements and safety utilization in the fields of industry and clean energy. It is a simple and effective way to achieve this goal utilizing the physical adsorption properties of stable porous metal–organic frameworks (MOFs). In this work, a stable self-interpenetrated three-dimensional MOF with a new 3,4-connected topology, {[Zn2(tpda)2(4,4′-bpy)]·4DMF}n (NKM-101; H2tpda = 4,4′-[4-(4H-1,2,4-triazol-4-yl)phenyl]dibenzoic acid, 4,4′-bpy = 4,4′-bipyridine, and DMF = N,N-dimethylformamide), has been successfully constructed based on a triazole–carboxyl ligand. The dense functional active sites existing on the inner walls of one-dimensional channels of NKM-101 are beneficial to enhancement of the binding affinities between the framework and specific molecules (CO2, C2–C4). Therefore, the selective adsorption and separation performance of the material on CO2/CH4 and C2–C4/CH4 are effectively improved. In addition, NKM-101 also exhibits excellent water stability, making it possible to be a practical material for the storage and purification of light hydrocarbons.

Dimethyl formamide

10.1021/acs.inorgchem.0c03618

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Citations (30)

Branch-Shaped Trapping Device Regulates Accelerated Catalyzed Hairpin Assembly and Its Application for MicroRNA In Situ Imaging

Analytical Chemistry (2022)

Huo Xu Yanhui Zheng Danlong Chen Yinghao Cheng Xiaojun Fang

Enzyme-free DNA strand displacement process is often practical when detecting miRNAs expressed at low levels in living cells. However, the poor kinetics, tedious reaction period, and multicomponent system hamper its in vivo applications to a great extent. Herein, we design a branch-shaped trapping device (BTD)-based spatial confinement reactor and applied it for accelerated miRNA in situ imaging. The reactor consists of a pair of trapped probe-based catalyzed hairpin assembly (T-CHA) reactions attached around the BTD. The trapping device naturally offered CHA reactions a good spatial-confinement effect by integrating the metastable probes (MHPa and MHPb) of the traditional CHA with the four-branched arm of BTD, which greatly improved the localized concentration of probes and shortened their physical distance. The autonomous and progressive walk of miRNA on the four-arm nanoprobes via T-CHA can rapidly tie numerous four-arm nanoprobes into figure-of-eight nanoknots (FENs), yielding strong fluorescence that is proportional to the miRNA expression level. The unique nanoarchitecture of the FEN also benefits the restricted freedom of movement (FOM) in a confined cellular environment, which makes the system ideally suitable for in situ imaging of intracellular miRNAs. In vitro and in situ analyses also demonstrated that the T-CHA overall outperformed the dissociative probe-based CHA (D-CHA) in stability, reaction speed, and amplification sensitivity. The final application of the T-CHA-based four-arm nanoprobe for imagings of both cancer cells and normal cells shows the potential of the platform for accurately and timely revealing miRNA in biological systems.

Nanoprobe

10.1021/acs.analchem.2c03956

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Citations (19)

Size and structure effects in the nanotoxic response of nanomaterials

Chinese Science Bulletin (2011)

Chang Xue

The biological effects and safety of nanomaterials are of particular significance in nanoscience, and are a key issue in the application of nanotechnology. Nanotechnology therefore needs breakthroughs in these two areas. First, precisely controllable and repeatable manufacturing processes must be developed on an industrial scale. Second, nanosafety knowledge and methodology must be developed, and safety assessment of nanoproducts must be given a regulatory framework. To prioritize the development of new technologies and to provide new for the global market, the development of safe products must be considered of equal importance to the development of leading technology. This has become a national strategy in many countries, and it is why nanotoxicology has quickly become a scientific discipline in the past five years. It is now known that the toxicological effects of nanomaterials are related to many of their properties. In this article, we focus primarily on two of the most important ones: nanosize and nanostructure. The dose-effects on nanotoxic responses are also briefly discussed. Particular focus is given to the differences in dose-effect relationships in traditional toxicology and nanotoxicology. Finally, we discuss the need for future research. Specifically, we identify the most important research topics, which urgently need to be understood, because of the rapid development of nanotechnology and its applications.

Nanotoxicology

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Bead-String-Shaped DNA Nanowires with Intrinsic Structural Advantages and Their Potential for Biomedical Applications

ACS Applied Materials & Interfaces (2019)

Yansha Gao Qian Li Jingjing Zhang Chengwei Wu Zhifa Shen

Due to high programmability and good biocompatibility, DNA has been recognized as a powerful building block for engineering of sophisticated nanostructures for different purposes. Herein, we present the first example of a bead-string-shaped DNA nanowire (BS-nanow) with long-range structural order for in vivo bioimaging and targeted drug delivery. BS-nanow is assembled from DNA tetrahedron units with precise nanometer-scale spatial control, capable of accommodating chemotherapeutic agents with high payload capacity (1204 binding sites) as well as possessing a 60-fold enhanced binding affinity for target cells. Confocal fluorescence imaging and in vivo experiments on CEM subcutaneous tumor-bearing mice show that specific bioimaging of living cells and even systemic delivery of drugs into internal tumor organs and tissues were accomplished, thereby achieving an efficient inhibition of tumor growth in the xenograft model without systemic toxicity. BS-nanow's show potential in vivo applications in accurate diagnosis and targeted therapy for human cancer.

Biocompatibility

DNA nanotechnology

Payload (computing)

10.1021/acsami.9b16249

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Citations (40)

Swelling of Serum-Stable DNA Nanoparticles upon Target-Induced Conformational Rearrangement of Sensing Probes for the Signal-On Detection of Cancer-Related Genes

Analytical Chemistry (2022)

Chang Xue Hong Huang Lei Wang Wenqiang Liao Hao Jiang

Nuclease-resistant assay probes are of significant importance for biochemical analysis and disease diagnosis. In this contribution, a reconfigurable lipidic moiety-attached DNA nanoparticle (LDN) is constructed from a cholesterol-conjugated multifunctional hairpin-type DNA probe (Chol-DP) by hydrophobicity-mediated self-assembly. The LDN holds high serum stability and displays a low false-positive signal even in a complex biological milieu. The hydrophobic cholesterol moiety enables the hydrophobicity-mediated assembly, while hydrophilic DNA sequence serves as a recognition element and a polymerization template. The initiator-activated strand displacement amplification (SDA) reaction can convert the hairpin-shaped probe into rigid double-stranded DNA (dsDNA), causing the conformational rearrangement-based LDN swelling that can be used to reliably and fluorescently signal the cancer-related p53 gene. The size increase and structural reconfiguration are confirmed by dynamic light scattering (DLS) analysis and confocal microscopy imaging, respectively. Target p53 is specifically detected down to 10 pM. The whole assay process involved only several simple mixing steps. Recovery test and blind test further confirm the feasibility of the use of the LDN for the detection of target DNA in a complex biological milieu, indicating a promising nanotool for biomedical applications.

Moiety

Nuclease

10.1021/acs.analchem.1c03598

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Citations (7)

Structure and optical properties of iron doped ZnSe microspheres

Optical Materials (2021)

Tingting Li Chengcheng Sun Chang Xue Yutao Jiang Jian Zhang

10.1016/j.optmat.2021.110989

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Citations (16)

Ultrathin 2D Ni‐UMOF Composites for Highly‐Efficient 4‐Nitrophenol Hydrogenation

ChemistrySelect (2019)

Xianfeng Yang Renjie Xiong Chang Xue Shuo Wang Zhu Ding

Abstract It is a challenge to synthesize metal‐organic frameworks (MOFs) catalysts with high catalytic activity and stability under reaction conditions. For this study, the combinations of two‐dimensional (2D) ultrathin nickel‐organic framework (Ni‐UMOF) with layered graphitic carbon nitride (g‐C 3 N 4 ) or graphene oxide (GO) as promising composite catalysts for the hydrogenation of 4‐nitrophenol were reported. The ultrathin nanosheet composites (Ni‐UMOF/g‐C 3 N 4 and Ni‐UMOF/GO) are obtained by a simple sol‐gel method and exhibit higher hydrogenation activity and recyclability than commercial Pd/C or Pt/C catalysts. The structures and properties of the catalysts are elucidated by X‐ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), atomic force microscope (AFM), fourier transform infrared spectra (FT‐IR), Raman, ultraviolet–visible spectroscopy (UV‐Vis), X‐ray photoelectron spectroscopy (XPS), electron spin‐resonance spectroscopy (ESR) and temperature‐programmed reduction (TPR). The results reveal that oxygen vacancies are produced on the surface of Ni‐UMOF composites and act as the dominating active sites during hydrogenation process. This sol‐gel method can be applied to synthesize other 2D ultrathin MOFs composites with high catalytic activities and recyclability for redox reactions.

Nanosheet

Graphitic carbon nitride

10.1002/slct.201904028

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Citations (5)