Xiaoliang Yao

George Washington University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Michael Keidar

George Washington University

Jonathan H. Sherman

Martinsburg VA Medical Center

Dayun Yan

George Washington University

Li Lin

George Washington University

Jilin Qi

Beijing University of Civil Engineering and Architecture

Qihui Wang

Institute of Microbiology

Vikas Soni

George Washington University

Lin Li

North China Electric Power University

Alisa Malyavko

George Washington University

Megan Kirschner

LMU Klinikum

Cooperative Institutions

George Washington University

Chinese Academy of Sciences

University of Chinese Academy of Sciences

Xi'an University of Technology

Memorial Sloan Kettering Cancer Center

Tsinghua University

University of Maryland, College Park

Yale University

Johns Hopkins University

Nanjing University of Science and Technology

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Influence of freeze–thaw on the stored free energy in soils

Cold Regions Science and Technology (2008)

Xiaoliang Yao Jilin Qi Wei Ma

Consolidation

Soil structure

10.1016/j.coldregions.2008.11.001

Cite

Citations (28)

Sensitization of glioblastoma cells to temozolomide by a helium gas discharge tube

Physics of Plasmas (2020)

Xiaoliang Yao Li Lin Vikas Soni Eda Gjika Jonathan H. Sherman

Glioblastoma is one of the most aggressive brain cancers. Chemotherapy is a standard modality for its therapy. Here, we demonstrated a novel strategy using a helium gas discharge tube as a tunable electromagnetic emission source to sensitize glioblastoma cells to the cytotoxicity of temozolomide (TMZ), a widely used drug for glioblastoma. After a single 7-min of treatment, the efficacy of TMZ was enhanced in two typical glioblastoma cell lines U87MG and A172 without affecting a normal human astrocyte cell line hTERT/E6/E7. The discharge tube is a non-invasive approach, which provides a safe, controllable, stable, and low-cost modality to improve the conventional chemotherapy.

Temozolomide

U87

10.1063/5.0017913

Cite

Citations (16)

Photolithographic-stereolithographic-tandem fabrication of 4D smart scaffolds for improved stem cell cardiomyogenic differentiation

Biofabrication (2018)

Shida Miao Haitao Cui Margaret Nowicki Se‐Jun Lee J. A. Afonso de Almeida

4D printing is a highly innovative additive manufacturing process for fabricating smart structures with the ability to transform over time. Significantly different from regular 4D printing techniques, this study focuses on creating novel 4D hierarchical micropatterns using a unique photolithographic-stereolithographic-tandem strategy (PSTS) with smart soybean oil epoxidized acrylate (SOEA) inks for effectively regulating human bone marrow mesenchymal stem cell (hMSC) cardiomyogenic behaviors. The 4D effect refers to autonomous conversion of the surficial-patterned scaffold into a predesigned construct through an external stimulus delivered immediately after printing. Our results show that hMSCs actively grew and were highly aligned along the micropatterns, forming an uninterrupted cellular sheet. The generation of complex patterns was evident by triangular and circular outlines appearing in the scaffolds. This simple, yet efficient, technique was validated by rapid printing of scaffolds with well-defined and consistent micro-surface features. A 4D dynamic shape change transforming a 2-D design into flower-like structures was observed. The printed scaffolds possessed a shape memory effect beyond the 4D features. The advanced 4D dynamic feature may provide seamless integration with damaged tissues or organs, and a proof of concept 4D patch for cardiac regeneration was demonstrated for the first time. The 4D-fabricated cardiac patch showed significant cardiomyogenesis confirmed by immunofluorescence staining and qRT-PCR analysis, indicating its promising potential in future tissue and organ regeneration applications.

10.1088/1758-5090/aabe0b

Cite

Citations (108)

[Intensifying the study of the proper treatment of digestive ulceration].

PubMed (1997)

Xiaoliang Yao

Source

Cite

Citations (0)

INNV-08. COMPARATIVE STUDY OF THE CANCER TREATMENT POTENTIAL EFFECT OF TUMOR-TREATING FIELDS AND COLD ATMOSPHERIC PLASMA

Neuro-Oncology (2020)

Xiaoliang Yao Isaac Goldstein Lin Li Jonathan H. Sherman Michael Keidar

Abstract INTRODUCTION Cold Atmospheric Plasma (CAP) is ionized gas close to room temperature that generates reactive oxygen and nitrogen species, an electromagnetic field (EMF) and UV, which are selectively cytotoxic to cancer cells. Tumor Treating Fields (TTFields) is an FDA approved cancer treatment that utilizes “low intensity” and “intermediate frequency” alternating electric fields to produce an inhibitory effect on cancerous cells. OBJECTIVES In this study, we directly compared CAP and TTFields treatments using U87 glioblastoma tumor cells. We aimed to assess the relative merits of both technologies as potential approaches for brain cancer treatment. METHODS In this in vitro study, the cells were treated with either CAP or TTFields at room temperature. In both cases in order the optimize the treatment parameters, the energy we applied was varied for both methods by changing the power and time of the treatment. The resulting cell viability changes were measured 72 hours after treatment. RESULTS The results revealed that increased energy resulted in decreased cell viability of U87 glioblastoma tumor cells with both CAP and TTFields treatment. The rate of U87 glioblastoma tumor cell viability under CAP treatment was 80% of the original viability after using 4.8J of energy, compared with 15,752J of energy with TTFields treatment. Additionally, the CAP displayed significantly greater time-efficiency as compared to TTFields. For instance, the most effective TTFields treatment took 4 hours, while the CAP treatment displayed a comparable effect on tumor cell viability with a treatment time of 30 seconds. CONCLUSIONS This preliminary in vitro study indicates that CAP treatment is potentially a more favorable effective method of brain cancer therapy. This data indicates that CAP is significantly more energy-efficient and requires significantly less treatment time than TTFields. Future research looks to compare the 2 treatments in an intracranial mouse xenograft model.

Viability assay

U87

10.1093/neuonc/noaa215.492

Cite

Citations (0)

Introducing adaptive cold atmospheric plasma: The perspective of adaptive cold plasma cancer treatments based on real-time electrochemical impedance spectroscopy

Physics of Plasmas (2020)

Li Lin Zichao Hou Xiaoliang Yao Yi Liu Jagadishwar R. Sirigiri

Following the understanding of the cold atmospheric plasma jet control, the optimization of plasma parameters for biomedical applications has become an important area of research in the field of plasma-based cancer treatment. A real-time feedback signal is usually required by a control algorithm, such as a self-adaptive plasma jet, which is designed to automatically self-optimize its parameters to adapt to a variety of biomedical applications and situations. In this paper, we introduce the potential of replacing the cell viability or cell stress assay with electrochemical impedance spectroscopy (EIS) to provide a real-time feedback signal for a model predictive control (MPC) method aided by machine learning. The EIS frequency is in the kHz to GHz regime. Therefore, the MPC method is not only designed for minimizing the cancer cell viability, but also considered to optimize cell membrane behaviors and other chemical species dialing. Since these signals are in the range of GHz, we introduce alternatives for the impedance analyzer to measure the impedance spectrum, including a Fabry–Pérot resonator and one of its scanning-array variations.

SIGNAL (programming language)

10.1063/5.0003528

Cite

Citations (32)

Pore Water Pressure Distribution and Dissipation During Thaw Consolidation

Transport in Porous Media (2016)

Xiaoliang Yao Jilin Qi Mengxin Liu Fan Yu

Consolidation

Effective stress

10.1007/s11242-016-0782-z

Cite

Citations (17)

Cell Membrane Oscillations under Radiofrequency Electromagnetic Modulation

Langmuir (2023)

Li Lin Marshall R. McCraw Berkin Uluutku Yi Liu Dayun Yan

Cell responses to external radiofrequencies (RF) are a fundamental problem of much scientific research, clinical applications, and even daily lives surrounded by wireless communication hardware. In this work, we report an unexpected observation that the cell membrane can oscillate at the nanometer scale in phase with the external RF radiation from kHz to GHz. By analyzing the oscillation modes, we reveal the mechanism behind the membrane oscillation resonance, membrane blebbing, the resulting cell death, and the selectivity of plasma-based cancer treatment based on the difference in the membrane's natural frequencies among cell lines. Therefore, a selectivity of treatment can be achieved by aiming at the natural frequency of the target cell line to focus the membrane damage on the cancer cells and avoid normal tissues nearby. This gives a promising cancer therapy that is especially effective in the mixing lesion of the cancer cells and normal cells such as glioblastoma where surgical removal is not applicable. Along with these new phenomena, this work provides a general understanding of the cell coupling with RF radiation from the externally stimulated membrane behavior to the cell apoptosis and necrosis.

Cell membrane

Oscillation (cell signaling)

10.1021/acs.langmuir.2c03181

Cite

Citations (8)

A New Physically Triggered Cell Death via Transbarrier Contactless Cold Atmospheric Plasma Treatment of Cancer Cells

arXiv (Cornell University) (2020)

Dayun Yan Qihui Wang Manish Adhikari Alisa Malyavko Lin Li

For years, extensive efforts have been made to discover effective, non-invasive anti-cancer therapies. Cold atmospheric plasma (CAP), is a near room temperature ionized gas composed of reactive species, charged particles, neutral particles, and electrons. CAP also has several physical factors including thermal radiation, ultraviolet (UV) radiation, and electromagnetic (EM) waves. Most of the previously reported biological effects of CAP have relied on direct contact between bulk plasma and cells, resulting in the chemical effects generally seen after CAP treatment. In this paper, we demonstrate that the electromagnetic emission produced by CAP can lead to the death of B16F10 melanoma cancer cells via a transbarrier contactless method. When compared with the effect of reactive species, the effect of the physical factors causes much greater growth inhibition. The physical-triggered growth inhibition is due to a new type of cell death, characterized by rapid leakage of bulk water from the cells, resulting in bubbles on the cell membrane, and cytoplasm shrinkage. The results of this study introduce a new possible mechanism of CAP induced cancer cell death and build a foundation for CAP to be used as a non-invasive cancer treatment in the future.

10.48550/arxiv.2004.14298

Cite

Citations (0)

Cold Atmospheric Plasma Devices for SARS-CoV-2 Sanitation on Solid Surfaces

Bulletin of the American Physical Society (2020)

Lin Li Kareem Kabra Xiaoliang Yao Christopher N. Mores Michael Keidar

2019-20 coronavirus outbreak

Source

Cite

Citations (0)