Abstract Background Clinical translation of therapeutic nuclear acid, particularly those targeting tumor progression, has been hampered by the intrinsic weaknesses of nuclear acid therapeutic including poor systemic stability, rapid clearance, low membrane permeability and lack of targeting ability. Small nuclear acid engineered into carrier-free nanodrugs with structural stability and disease targeting may be viable to overcome pharmaceutical obstacles of nuclear acid. Methods A general method through a mild and simple chemistry was established to convert therapeutic miRNA into an infinite Auric-sulfhydryl coordination supramolecular miRNA termed IacsRNA with near-spherical nanostructure, high colloid as well as anti-hydrolysis stability and low macrophage uptakes. Results IacsRNA presented the increased half-life period in circulation and accumulation at tumor sites in comparison to normal miRNA. Moreover, Iacs-miR-30c showed no toxicity of viscera and sanguis system in the 5-time injection dosage of the treatment. More importantly, Iacs-miR-30c potently suppressed the Wnt signaling pathway in vitro and in vivo, and effectively sensitized both potency of 5-Fu in PDX model of colon cancer and Anti-PD1 in B16F10 homograft model of melanoma. Conclusion Collectively, this work amply confirmed the design of IacsRNA as a general and viable strategy of nano-pharmaceutic to concert flimsy therapeutic miRNA into potential drugs. Considering from a broader perspective, the miRNA-initiated infinite coordination self-assembly strategy has distinct advantages in resurrecting nuclear acid therapeutics, probably bringing new inspiration to RNA-derived therapeutics of a great variety of human diseases including cancer. Graphical Abstract
The effect of Ga (0～2 wt%)on microstructure and magnetic properties of sintered Nd_(15)Fe_(78)B_7 was studied.The results show that intrinsic coercivity can be increased with the same B_r and (BH)_(max) by adding some Ga.The microstructure is close to ideal structure with 1.0 wt% Ga.Ga exists in the forms of Nd_2Fe_(14-x)Ga_xB,and GaNd in the magnets. More than 1.5 wt% Ga makes Nd_2Fe_(14)B unstable.
This paper studies the evolution of wave in the system of a pure anharmonic lattice with a double well on-site potential by numerical calculation. It finds that an initial distribution of static or moving wave can evolve into two travelling soliton-like trains with contrary directions and a region of oscillation in this lattice system. It presents that some cases with cosine-square-shape and Gaussian-shape initial distribution of static or moving wave will produce ordered soliton-like train. Careful numerical observation shows that the centre oscillation region in this system may act as a resource of generating soliton-like train.
The modern preparation techniques for nanocrystalline rareearth magnets are reviewed.The principal synthesis methods include melt spinning,mechanical alloying,hydrogenation-disproportionation-dekydrogention-recombination(HDDR),magnetron sputtering and hot deformation.These techniques are used for NdFeB-,PrFeB-SmFeN-and SmCo-type systems in order to improve the magnetic properties.
To improve the teaching effect,the tissue chips were used to reform the teaching models,and its effect was evaluated by analyzing the histology exam results and comparing with the traditional exam results by traditional slices.The results showed that tissue chips in the histological experiment saved time and manpower,inspired the students' learning interest and thinking ability,improved the teaching effect and evaluated the students' ability of using microscope.Tissue chips have the advantages of low cost,high comparability,simplicity and low limitation,which are more beneficial to improve the teaching quality of experimental teaching of histology.
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