Xiaoning He

Fourth Affiliated Hospital of China Medical University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Jing Wu

The Seventh Affiliated Hospital of Sun Yat-sen University

Moussa Benhamed

Université Paris Cité

yuanjia he

China Medical University

David Latrasse

Centre National de la Recherche Scientifique

Cécile Raynaud

Institut des Sciences des Plantes de Paris Saclay

Xue Yuan

Indiana University – Purdue University Indianapolis

Clara Bergis

Institut des Sciences des Plantes de Paris Saclay

Shuying Yang

Taiyuan Normal University

Rim Brik-Chaouche

Centre National de la Recherche Scientifique

Haibo Xue

Binzhou University

Cooperative Institutions

Centre National de la Recherche Scientifique

Institut des Sciences des Plantes de Paris Saclay

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Université Paris-Saclay

Sun Yat-sen University

Université Paris Cité

Sorbonne Paris Cité

Northwest A&F University

Chinese Academy of Sciences

China Medical University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

HSFA1a modulates plant heat stress responses and alters the 3D chromatin organization of enhancer-promoter interactions

Nature Communications (2023)

Ying Huang Jing An Sanchari Sircar Clara Bergis Chloé Dias Lopes

Abstract The complex and dynamic three-dimensional organization of chromatin within the nucleus makes understanding the control of gene expression challenging, but also opens up possible ways to epigenetically modulate gene expression. Because plants are sessile, they evolved sophisticated ways to rapidly modulate gene expression in response to environmental stress, that are thought to be coordinated by changes in chromatin conformation to mediate specific cellular and physiological responses. However, to what extent and how stress induces dynamic changes in chromatin reorganization remains poorly understood. Here, we comprehensively investigated genome-wide chromatin changes associated with transcriptional reprogramming response to heat stress in tomato. Our data show that heat stress induces rapid changes in chromatin architecture, leading to the transient formation of promoter-enhancer contacts, likely driving the expression of heat-stress responsive genes. Furthermore, we demonstrate that chromatin spatial reorganization requires HSFA1a, a transcription factor (TF) essential for heat stress tolerance in tomato. In light of our findings, we propose that TFs play a key role in controlling dynamic transcriptional responses through 3D reconfiguration of promoter-enhancer contacts.

Reprogramming

ChIA-PET

10.1038/s41467-023-36227-3

Cite

Citations (57)

BMP2 Genetically Engineered MSCs and EPCs Promote Vascularized Bone Regeneration in Rat Critical-Sized Calvarial Bone Defects

PLoS ONE (2013)

Xiaoning He Rosemary Dziak Xue Yuan Keya Mao Robert J. Genco

Current clinical therapies for critical-sized bone defects (CSBDs) remain far from ideal. Previous studies have demonstrated that engineering bone tissue using mesenchymal stem cells (MSCs) is feasible. However, this approach is not effective for CSBDs due to inadequate vascularization. In our previous study, we have developed an injectable and porous nano calcium sulfate/alginate (nCS/A) scaffold and demonstrated that nCS/A composition is biocompatible and has proper biodegradability for bone regeneration. Here, we hypothesized that the combination of an injectable and porous nCS/A with bone morphogenetic protein 2 (BMP2) gene-modified MSCs and endothelial progenitor cells (EPCs) could significantly enhance vascularized bone regeneration. Our results demonstrated that delivery of MSCs and EPCs with the injectable nCS/A scaffold did not affect cell viability. Moreover, co-culture of BMP2 gene-modified MSCs and EPCs dramatically increased osteoblast differentiation of MSCs and endothelial differentiation of EPCs in vitro. We further tested the multifunctional bone reconstruction system consisting of an injectable and porous nCS/A scaffold (mimicking the nano-calcium matrix of bone) and BMP2 genetically-engineered MSCs and EPCs in a rat critical-sized (8 mm) caviarial bone defect model. Our in vivo results showed that, compared to the groups of nCS/A, nCS/A+MSCs, nCS/A+MSCs+EPCs and nCS/A+BMP2 gene-modified MSCs, the combination of BMP2 gene -modified MSCs and EPCs in nCS/A dramatically increased the new bone and vascular formation. These results demonstrated that EPCs increase new vascular growth, and that BMP2 gene modification for MSCs and EPCs dramatically promotes bone regeneration. This system could ultimately enable clinicians to better reconstruct the craniofacial bone and avoid donor site morbidity for CSBDs.

10.1371/journal.pone.0060473

Cite

Citations (100)

Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation

Nature Communications (2016)

Xue Yuan Jay Cao Xiaoning He Rosa Serra Jun Qu

Abstract Intraflagellar transport proteins (IFT) are required for hedgehog (Hh) signalling transduction that is essential for bone development, however, how IFT proteins regulate Hh signalling in osteoblasts (OBs) remains unclear. Here we show that deletion of ciliary IFT80 in OB precursor cells (OPC) in mice results in growth retardation and markedly decreased bone mass with impaired OB differentiation. Loss of IFT80 blocks canonical Hh–Gli signalling via disrupting Smo ciliary localization, but elevates non-canonical Hh–Gαi–RhoA–stress fibre signalling by increasing Smo and Gαi binding. Inhibition of RhoA and ROCK activity partially restores osteogenic differentiation of IFT80 -deficient OPCs by inhibiting non-canonical Hh–RhoA–Cofilin/MLC2 signalling. Cytochalasin D, an actin destabilizer, dramatically restores OB differentiation of IFT80 -deficient OPCs by disrupting actin stress fibres and promoting cilia formation and Hh–Gli signalling. These findings reveal that IFT80 is required for OB differentiation by balancing between canonical Hh–Gli and non-canonical Hh–Gαi–RhoA pathways and highlight IFT80 as a therapeutic target for craniofacial and skeletal abnormalities.

Intraflagellar Transport

Smoothened

10.1038/ncomms11024

Cite

Citations (114)

Advances in Antipsychotics

Elisabeth Dowling Root Xiaoning He Stephanie A. Flowers Martin A. Katzman Tsuruhei Sukegawa

Antipsychotics are a class of psychiatric medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally for schizophrenia and bipolar disorder, and are increasingly being used in the management of non-psychotic disorders (ATC code N05A). First-generation antipsychotics, known as typical antipsychotics, neuroleptics or major tranquilizers, were discovered in the 1950s. Most second-generation drugs, known as atypical antipsychotics, have been developed more recently. Both generations of medication tend to block receptors in the brain’s dopamine pathways, but atypicals tend to act on serotonin receptors as well. In the present book, ten typical literatures about antipsychotics published on international authoritative journals were selected to introduce the worldwide newest progress, which contains reviews or original researches on medical science, neurology, psychology, ect. We hope this book can demonstrate advances in antipsychotics as well as give references to the researchers, students and other related people.

Psychopharmacology

Source

Cite

Citations (0)

The co-culture of ASCs and EPCs promotes vascularized bone regeneration in critical-sized bone defects of cranial bone in rats

Stem Cell Research & Therapy (2020)

yuanjia he Shuang Lin Qiang Ao Xiaoning He

Abstract Background The repair of critical-sized bone defect represents a challenging problem in bone tissue engineering. To address the most important problem in bone defect repair, namely insufficient blood supply, this study aimed to find a method that can promote the formation of vascularized bone tissue. Method The phenotypes of ASCs and EPCs were identified respectively, and ASCs/EPCs were co-cultured in vitro to detect the expression of osteogenic and angiogenic genes. Furthermore, the co-culture system combined with scaffold material was used to repair the critical-sized bone defects of the cranial bone in rats. Results The co-culture of ASCs/EPCs could increase osteogenesis and angiogenesis-related gene expression in vitro. The results of in vivo animal experiments demonstrated that the ASC/EPC group could promote bone regeneration and vascularization in the meantime and then significantly accelerate the repair of critical-sized bone defects. Conclusion It is feasible to replace traditional single seed cells with ASC/EPC co-culture system for vascularized bone regeneration. This system could ultimately enable clinicians to better repair the defect of craniofacial bone and avoid donor site morbidity.

10.1186/s13287-020-01858-6

Cite

Citations (25)

PDB131 INSULIN TREATMENT PATTERN, RESOURCE UTILIZATION AND COSTS IN CHINESE PATIENTS WITH TYPE 2 DIABETES MELLITUS INITIATED BASAL OR PREMIXED INSULIN ANALOGS AFTER INSULIN INTENSIFICATION

Value in Health (2019)

C. Liu Xiaoning He K. Wang Hong Chen Jing Wu

Discontinuation

Basal (medicine)

10.1016/j.jval.2019.04.678

Cite

Citations (0)

Use of Anti-Diabetic Medication is Not Associated with Cancer Risk in Chinese Patients with Type 2 Diabetes

Value in Health (2018)

Xiaoning He Lizheng Shi Jing Wu

10.1016/j.jval.2018.04.119

Cite

Citations (0)

Development of cloned embryos from porcine neural stem cells and amniotic fluid-derived stem cells transfected with enhanced green fluorescence protein gene

Reproduction (2009)

Yue‐Mao Zheng Huiying Zhao Xiaoe Zhao Fusheng Quan Hua Song

We assessed the developmental ability of embryos cloned from porcine neural stem (NS) cells, amniotic fluid-derived stem (AFS) cells, fetal fibroblast cells, adult fibroblast, and mammary gland epithelial cells. The five cell lines were transfected with enhanced green fluorescence protein gene respectively using lipofection. NS and AFS cells were induced to differentiate in vitro. Stem cells and their differentiated cells were harvested for analysis of the markers using RT-PCR. The five cell lines were used for nuclear transfer. The two-cell stage-cloned embryos derived from each cell line were transferred into the oviducts of surrogate mothers. The results showed that both NS and AFS cells expressed POU5F1, THY1 and SOX2, and they were both induced to differentiate into astrocyte (GFAP+), oligodendrocyte (GalC+), neuron (NF+, ENO2+, and MAP2+), adipocyte (LPL+ and PPARG-D+), osteoblast (osteonectin+ and osteocalcin+), myocyte (MYF6+ and MYOD+), and endothelium (PECAM1+, CD34+, CDH5+, and NOS3+) respectively. Seven cloned fetuses (28 days and 32 days) derived from stem cells were obtained. The in vitro developmental ability (morula-blastocyst rate was 28.26-30.07%) and in vivo developmental ability (pregnancy rate were 1.67-2.17%) of the embryos cloned from stem cells were higher (P<0.05) than that of the embryos cloned from somatic cells (morula-blastocyst rate was 16.27-19.28% and pregnancy rate was 0.00%), which suggests that the undifferentiated state of the donor cells increases cloning efficiency.

Neurosphere

Amniotic stem cells

Perivitelline space

10.1530/rep-08-0469

Cite

Citations (26)

Enhancing the Interpretation of Continuous Outcomes and Subgroup Analyses in Systematic Reviews

JAMA Pediatrics (2024)

Jing Wu Qi Wang Xiaoning He

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Journals JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2024 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal

10.1001/jamapediatrics.2024.0720

Cite

Citations (1)

Health economic assessment of Gd-EOB-DTPA MRI versus ECCM-MRI and multi-detector CT for diagnosis of hepatocellular carcinoma in China

PLoS ONE (2018)

Xiaoning He Jing Wu Anke‐Peggy Holtorf Harald Rinde Shuangshuang Xie

Limited data exists in China on the comparative cost of gadolinium ethoxybenzyl diethylenetriamine magnetic resonance imaging (Gd-EOB-DTPA-MRI) with other imaging techniques. This study compared the total cost of Gd-EOB-DTPA-MRI with multidetector computed tomography (MDCT) and extracellular contrast media–enhanced MRI (ECCM-MRI) as initial imaging procedures in patients with suspected hepatocellular carcinoma (HCC). We developed a decision-tree model on the basis of the Chinese clinical guidelines for HCC, which was validated by clinical experts from China. The model compared the diagnostic accuracy and costs of alternative initial imaging procedures. Compared with MDCT and ECCM-MRI, Gd-EOB-DTPA-MRI imaging was associated with higher rates of diagnostic accuracy, i.e. higher proportions of true positives (TP) and true negatives (TN) with lower false positives (FP). Total diagnosis and treatment cost per patient after the initial Gd-EOB-DTPA-MRI evaluation was similar to MDCT (¥30,360 vs. ¥30,803) and lower than that reported with ECCM-MRI (¥30,360 vs. ¥31,465). Lower treatment cost after initial Gd-EOB-DTPA-MRI was driven by reduced utilization of confirmatory diagnostic procedures and unnecessary treatments. The findings reported that Gd-EOB-DTPA-MRI offered higher diagnostic accuracy compared with MDCT and ECCM-MRI at a comparable cost, which indicates Gd-EOB-DTPA-MRI could be the preferred initial imaging procedure for the diagnosis of HCC in China.

10.1371/journal.pone.0191095

Cite

Citations (15)