Zhaoliang Liu

Soochow University

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i-10 index

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Ning Li

Xiamen University

Jianming Xu

Baylor College of Medicine

Shuang Yang

Chinese Academy of Medical Sciences & Peking Union Medical College

Zhenhua Wu

Huaiyin Institute of Technology

Mingming Xu

Fujian Medical University

Xiaoxiang Hu

China Agricultural University

Xumin Zhang

Fudan University

Qin Li

South China Normal University

Jianming Xu

Chinese People's Liberation Army

Hong-Wu Chen

Shantou University

Cooperative Institutions

China Agricultural University

Soochow University

Baylor College of Medicine

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Sciences

Capital Medical University

Shanghai Jiao Tong University

Second Affiliated Hospital of Soochow University

University of Chinese Academy of Sciences

Southern Medical University

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Different mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells

The Journal of Nutritional Biochemistry (2010)

Jiajie Zhai Zhaoliang Liu Jie-mei Li Jianping Chen Lin Jiang

Perilipin

UCP3

Thermogenin

Fatty Acid Metabolism

Lipid droplet

10.1016/j.jnutbio.2009.09.007

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Citations (31)

Reprogramming the posttranslational code of SRC-3 confers a switch in mammalian systems biology

Proceedings of the National Academy of Sciences (2010)

Brian York Chundong Yu Jørn V. Sagen Zhaoliang Liu Bryan C. Nikolai

Here we demonstrate that reprogramming steroid receptor coactivator-3 (SRC-3) function by changing its posttranslational modification (PTM) code drastically influences systems biology. These findings support the physiological importance of PTMs in directing in vivo functions of a master coregulator. We previously reported that the transactivation potential of SRC-3 is controlled in part by PTMs, although this data emanated from in vitro studies. To test the physiological implications of PTMs on SRC-3, we developed a knock-in mouse model containing mutations at four conserved phosphorylation sites. These mice displayed a systems biology phenotype with increased body weight and adiposity, coupled with reduced peripheral insulin sensitivity. Collectively, these phenotypes result from increased IGF1 signaling, due to elevated IGFBP3 levels. We provide convincing evidence that these mutations in SRC-3 promoted enhanced transcription of the IGFBP3 gene and globally influenced growth and metabolism. Consequently, these mice displayed increased liver tumorigenesis, which likely results from elevated IGF1 signaling.

Reprogramming

10.1073/pnas.1005262107

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Citations (41)

Identification and analysis of a novel microsatellite marker flanking porcine myostatin gene (MSTN)

Acta Genetica Sinica (2004)

Yunliang Jiang Ning Li Xing-Bo Zhao Xiaoxiang Hu Zhaoliang Liu

Subcloning

Myostatin

Source

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Citations (3)

Construction and Characterization of a Novel 13.34‐Fold Chicken Bacterial Artificial Chromosome Library

Animal Biotechnology (2003)

Wei Liu Zhaoliang Liu Xiaoxiang Hu Ying Zhang Jing Yuan

A chicken bacterial artificial chromosome (BAC) library consisting of 138,240 clones was constructed in vector pBeloBAC11 with genomic DNA isolated from female white-silk chicken. An average insert size of 118 kb was estimated from 452 randomly isolated clones, which indicate the library to be approximate 13.34-fold genome coverage. For the demonstration of the probability to pick out any unique genes or DNA markers from the library, 8 single-copy genes were screened out and the positive clones were yielded between 2 and 15 with an average of 11.125, in agreement with the estimated high genomic coverage of this library. Positive superpools were obtained for 40 microsatellite markers selected from different regions of chicken genome. The number of positive superpools for each marker varies from 1 to 15 with an average of 9.475.

Bacterial artificial chromosome

genomic DNA

Insert (composites)

10.1081/abio-120026484

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Citations (14)

[Cloning and sequencing of fatty acid binding proteins gene in chicken].

PubMed (2002)

Qigui Wang Ning Li Xuemei Deng Zhiliang Gu Zhaoliang Liu

Homology

Cloning (programming)

Source

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Citations (4)

Novel Approach to Enriching Glycosylated RNAs: Specific Capture of GlycoRNAs via Solid-Phase Chemistry

Analytical Chemistry (2023)

Jiajia Li Shuang Yue Ziyuan Gao Wenhua Hu Zhaoliang Liu

Ribonuclease (RNA) modifications can alter cellular function and lead to differential immune responses by acting as discriminators between RNAs from different phyla. RNA glycosylation has recently been observed at the cell surface, and its dysregulation in disease may change RNA functions. However, determining which RNA substrates can be glycosylated remains to be explored. Here, we develop a solid-phase chemoenzymatic method (SPCgRNA) for targeting glycosylated RNAs, by which glycosylated RNA substrates can be specifically recognized. We found the differential N-glycosylation of small RNAs in hTERT-HPNE and MIA PaCa-2 cancer cells using SPCgRNA. RNA-Seq showed that the changes in glyco-miRNAs prepared from SPCgRNA were consistent with those of traditional methods. The KEGG signaling pathway analysis revealed that differential miRNA glycosylation can affect tumor cell proliferation and survival. Further studies found that NGI-1 significantly inhibited the proliferation, migration, and circulation of MIA PaCa-2 and promoted cell apoptosis. In addition, β-1,4-galactosyltransferase 1 (B4GALT1) not only affected the expression level of glycosylated miRNAs hsa-miR-21-5p but also promoted cell apoptosis and inhibited the cell cycle possibly through the p53 signaling pathway, while B4GALT1 and p53 were also affected following the hsa-miR-21-5p increase. These results suggest that B4GALT1 may catalyze miRNAs glycosylation, which further promotes cancer cell progression.

KEGG

Circular RNA

10.1021/acs.analchem.3c01630

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Citations (20)

Physical mapping of the giant panda immunoglobulin heavy chain constant region genes

Developmental & Comparative Immunology (2007)

Zhihui Zhao Yaofeng Zhao Qiang Pan‐Hammarström Wei Liu Zhaoliang Liu

Immunoglobulin heavy chain

10.1016/j.dci.2007.01.002

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Citations (17)

Glycoproteomic Bioanalysis of Exosomes by LC–MS for Early Diagnosis of Pancreatic Cancer

Bioanalysis (2021)

Mingming Xu Wenhua Hu Zhaoliang Liu Jun Xia Sufeng Chen

10.4155/bio-2021-0036

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Citations (15)

Supplementary Methods, Figures 1-5 from The Steroid Receptor Coactivator-1 Regulates Twist Expression and Promotes Breast Cancer Metastasis

Qin Li Zhaoliang Liu Hongwu Chen Jianming Xu

Supplementary Methods, Figures 1-5 from The Steroid Receptor Coactivator-1 Regulates Twist Expression and Promotes Breast Cancer Metastasis

Breast cancer metastasis

Expression (computer science)

10.1158/0008-5472.22381268

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Citations (0)

Hydrophilic Peptide and Glycopeptide as Immobilized Sorbents for Glycosylation Analysis

Analytical Chemistry (2024)

Zhaoliang Liu Zhenhua Wu Yufeng Zhou Jun Xia Wenqi Zhang

Hydrophilic interaction liquid chromatography (HILIC) is widely used for glycopeptide enrichment in shot-gun glycoproteomics to enhance the glycopeptide signal and minimize the ionization competition of peptides. In this work, we have developed a novel hydrophilic material (glycoHILIC) based on glycopeptides and peptides to provide hydrophilic properties. GlycoHILIC was synthesized by oxidizing cotton and then reacting the resulting aldehyde with the N-terminus of the glycopeptide or peptide by reductive amination. Due to the large amount of hydrophilic carbohydrates and hydrophilic amino acids contained in glycopeptides, glycoHILIC showed significantly better enrichment of glycopeptides than cotton itself. Our results demonstrate that glycoHILIC has high selectivity, a low detection limit, and good stability. Over 257 unique N-linked glycosylation sites in 1477 intact N-glycopeptides from 146 glycoproteins were identified from 1 μL of human serum using glycoHILIC. Serum analysis of pancreatic cancer patients found that 38 N-glycopeptides among 21 glycoproteins changed significantly, of which 7 N-glycopeptides increased and 31 N-glycopeptides decreased. These results demonstrate that glycoHILIC can be used for glycopeptide enrichment and analysis.

Glycoproteomics

Reductive amination

10.1021/acs.analchem.3c03944

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Citations (10)