Rihan Wu

Sun Yat-sen University

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Gerelt Borjigin

Inner Mongolia Agricultural University

Xige He

Inner Mongolia University

Yueying Yun

Inner Mongolia University of Science and Technology

Jindi Wu

Inner Mongolia Agricultural University

Lina Sha

Sichuan Agricultural University

Xia Qin

Inner Mongolia Agricultural University

Yunfei Han

Nanjing University of Chinese Medicine

Tadayuki Nishiumi

Niigata University

Lu Chen

Inner Mongolia Agricultural University

Yang Liu

Sichuan Agricultural University

Cooperative Institutions

Inner Mongolia Agricultural University

Sun Yat-sen University

The First Affiliated Hospital, Sun Yat-sen University

Chinese Academy of Sciences

Sichuan University

Cornell University

Key Laboratory of Guangdong Province

Shanghai Jiao Tong University

Central South University

Huazhong University of Science and Technology

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The effects of modified RNA-binding proteins HuR on the biological behavior of the bladder cancer T24 cell line

Translational Andrology and Urology (2022)

Qiang Ma Xu Chen Xiaomin Han Xinxin Wang Wei Zhang

Qiang Ma, Chen Xu, Xiaomin Han, Xinxin Wang, Wei Zhang, Zhi Liu, Rihan Wu, Fei Wu, Xiaohui Liu, Tengteng Zhang, Yan Su, Kewen Zheng, Yukun Wang

Viability assay

10.21037/tau-22-123

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Integrated Transcriptome Analysis of miRNAs and mRNAs in the Skeletal Muscle of Wuranke Sheep

Genes (2023)

Yueying Yun Rihan Wu Xige He Xia Qin Lu Chen

MicroRNAs (miRNAs) are regarded as important regulators in skeletal muscle development. To reveal the regulatory roles of miRNAs and their target mRNAs underlying the skeletal muscle development of Wuranke sheep, we investigated the miRNA and mRNA expression profiles in the biceps femoris of these sheep at the fetal (3 months of gestation) and 3- and 15-month-old postnatal stages. Consequently, a total of 1195 miRNAs and 24,959 genes were identified. Furthermore, 474, 461, and 54 differentially expressed miRNAs (DEMs) and 6783, 7407, and 78 differentially expressed genes (DEGs) were detected among three comparative groups. Functional analysis demonstrated that the target mRNAs of the DEMs were enriched in multiple pathways related to muscle development. Moreover, the interactions among several predicted miRNA–mRNA pairs (oar-miR-133-HDAC1, oar-miR-1185-5p-MYH1/HADHA/OXCT1, and PC-5p-3703_578-INSR/ACTG1) that potentially affect skeletal muscle development were verified using dual-luciferase reporter assays. In this study, we identified the miRNA and mRNA differences in the skeletal muscle of Wuranke sheep at different developmental stages and revealed that a series of candidate miRNA–mRNA pairs may act as modulators of muscle development. These results will contribute to future studies on the function of miRNAs and their target mRNAs during skeletal muscle development in Wuranke sheep.

RNA-Seq

10.3390/genes14112034

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Transcriptome analysis of messenger RNA and long noncoding RNA related to different developmental stages of tail adipose tissues of sunite sheep

Food Science & Nutrition (2021)

Xige He Rihan Wu Yueying Yun Xia Qin Lu Chen

Abstract The tail fat of sheep is the most typical deposited fat, and it can be widely used in human daily life, such as diet, cosmetics, and industrial raw materials. To understand the potential regulatory mechanism of different growth stages of tail fat in Sunite sheep, we performed high‐throughput RNA sequencing to characterize the long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression profiles of the sheep tail fat at the age of 6, 18, and 30 months. A total of 223 differentially expressed genes (DEGs) and 148 differentially expressed lncRNAs were found in the tail fat of 6‐, 18‐, and 30‐month‐old sheep. Based on functional analysis, we found that fat‐related DEGs were mainly expressed at 6 months of age and gradually decreased at 18 and 30 months of age. The target gene prediction analysis shows that most of the lncRNAs target more than 20 mRNAs as their transregulators. Further, we obtained several fat‐related differentially expressed target genes; these target genes interact with different differentially expressed lncRNAs at various ages and play an important role in the development of tail fat. Based on the DEGs and differentially expressed lncRNAs, we established three co‐expression networks for each comparison group. Finally, we concluded that the development of the sheep tail fat is more active during the early stage of growth and gradually decreases with the increase in age. The mutual regulation of lncRNAs and mRNAs may play a key role in this complex biological process.

RNA-Seq

10.1002/fsn3.2537

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Expression of myogenic regulatory factor genes in skeletal muscle satellite cells from Wuzhumuqin sheep in vitro

Small Ruminant Research (2020)

Rihan Wu Yueying Yun Feng Qinghui Xia Qin Xige He

10.1016/j.smallrumres.2020.106251

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Relationships among muscle fiber type composition, fiber diameter and MRF gene expression in different skeletal muscles of naturally grazing Wuzhumuqin sheep during postnatal development

Animal Science Journal (2017)

Qimuge Siqin Tadayuki Nishiumi Takahisa Yamada Shuiqing Wang Wenjun Liu

Abstract The aim of this study was to determine the relationships among muscle fiber‐type composition, fiber diameter, and myogenic regulatory factor ( MRF) gene expression in different skeletal muscles during development in naturally grazing Wuzhumuqin sheep. Three major muscles (i.e. the Longissimus dorsi ( LD ), Biceps femoris ( BF ) and Triceps brachii ( TB )) were obtained from 20 Wuzhumuqin sheep and 20 castrated rams at each of the following ages: 1, 3, 6, 9, 12 and 18 months. Muscle fiber‐type composition and fiber diameter were measured using histochemistry and morphological analysis, and MRF gene expression levels were determined using real‐time PCR . In the LD muscle, changes in the proportion of each of different types of fiber (I, IIA and IIB ) were relatively small. In the BF muscle, a higher proportion of type I and a 6.19‐fold lower proportion of type IIA fibers were observed ( P < 0.05). In addition, the compositions of type I and IIA fibers continuously changed in the TB muscle ( P < 0.05). Moreover, muscle diameter gradually increased throughout development ( P < 0.05). Almost no significant difference was found in MRF gene expression patterns, which appeared to be relatively stable. These results suggest that changes in fiber‐type composition and increases in fiber size may be mutually interacting processes during muscle development.

Fiber type

Muscle fibre

Longissimus dorsi

10.1111/asj.12848

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Citations (23)

Differential expression and localisation of heat shock protein 70 (HSP70) and glutathione peroxidase 5 (GPX5) in the testis and epididymis of Sonid Bactrian camels

Reproduction Fertility and Development (2023)

Gaowa Hasi Liyasu Wu Tserennadmid Sodnompil Ruhan Yi Rihan Wu

Heat shock protein 70 (HSP70) and glutathione peroxidase 5 (GPX5) are biomarkers of oxidative stress and stress in temperate, tropical environments, which are crucial for male reproduction. Their expression and distribution patterns in the testis and epididymis of Bactrian camels are still unknown.This study aims to investigate the HSP70 and GPX5 expression and localisation in 3- and 6-year-old Bactrian camel testis and epididymis.Reverse transcription quantitative polymerase chain reaction (qRT-PCR), Western blot and immunohistochemistry were used to detect HSP70 in the testis and epididymis (caput, corpus and cauda) and GPX5 in the epididymis at two developmental stages (3-year-old puberty group and 6-year-old adult group).HSP70 was upregulated in the testis. Immunohistochemistry results indicated the HSP70 protein was mainly detected in spermatids and Leydig cells of testicular tissue. In the epididymis, HSP70 was located at the luminal spermatozoa, the epithelium lining the epididymal and the epididymal interstitium. GPX5 expression was significantly higher in the caput epididymis than in the corpus and cauda epididymis. GPX5 protein was observed in the epithelium lining the epididymal, interstitium and luminal spermatozoa in the epididymis by immunohistochemistry.Bactrian camel HSP70 and GPX5 exhibited spatiotemporal expression specificity.HSP70 and GPX5 may be essential for germ cell development and reproductive success after sexual maturation in Sonid Bactrian camels.

10.1071/rd23026

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AMH inhibits androgen production in human theca cells

The Journal of Steroid Biochemistry and Molecular Biology (2022)

Ming‐Hui Chen Xi Guo Yiping Zhong Yang Liu Cai Bing

CYP17A1

10.1016/j.jsbmb.2022.106216

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Citations (9)

Luteinizing hormone stimulates the expression of amphiregulin in human theca cells

Journal of Ovarian Research (2022)

Yang Liu Yiping Zhong Xiaoting Shen Xi Guo Rihan Wu

Luteinizing hormone (LH) can stimulate mural granulosa cells to produce Amphiregulin (AREG), which can induce the resumption of meiosis in oocytes. Theca cells are present in the outer layer of follicles, providing communication with the pituitary axis through the established vascular system around the follicle. As LH target cells, it is unknown whether theca cells can produce AREG after LH stimulation.Primary cultured human theca cells were treated with LH (with or without the inhibitor of PKA, H89), or agonists of adenylate cyclase (forskolin or db-cAMP). The mRNA and protein levels of AREG were evaluated by RT-qPCR, immunochemistry, immunofluorescence, western blotting, and ELISA.Immunohistochemistry of normal ovarian tissue obtained in the early-mid follicle phase showed that AREG expression was absent in both the theca layer and the granulosa cell layer of antral follicles. Double immunofluorescent staining revealed colocalization of AREG and CYP17A1 in human theca cells and colocalization of FSHR and AREG in human granulosa cells isolated from follicular fluid collected during IVF/ICSI after hCG trigger. LH significantly increased the mRNA and protein levels of AREG in human theca cells and the concentration of AREG in the culture medium. Forskolin and db-cAMP, activators of the cAMP/PKA signalling pathway, also significantly increased the mRNA and protein levels of AREG in human theca cells and the concentration of AREG in the culture medium. H89 antagonized the stimulating effect of LH on AREG expression in human theca cells. In addition, the concentration of AREG was lower in polycystic ovarian syndrome (PCOS) follicular fluid than in normal follicular fluid. The mRNA levels of AREG were significantly lower in PCOS granulosa cells and theca cells than in normal granulosa cells and theca cells.LH can stimulate the expression of AREG in human theca cells, and the adenylate cyclase/cAMP/PKA cascade may mediate this process. Expression of AREG is decreased in PCOS theca cells compared to normal theca cells, with or without LH stimulation.

Follistatin

10.1186/s13048-022-01062-5

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Citations (2)

Transcriptome analysis of messenger RNA and long noncoding RNA related to different developmental stages of tail adipose tissues of sunite sheep

Research Square (Research Square) (2020)

Xige He Rihan Wu Yueying Yun Xia Qin Lu Chen

Abstract Background: Sunite sheep are a fat-tailed sheep species with a low percentage of intramuscular fat and good quality lean meat, and their tail fat can be used as a source of dietary fat by humans. To understand the potential regulatory mechanism of different growth stages of tail fat in Sunite sheep, we performed high-throughput RNA sequencing to characterize the long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression profiles of the sheep tail fat at the age of 6 months, 18 months, and 30 months. Results: A total of 223 differentially expressed genes (DEGs) and 148 differentially expressed lncRNAs were found in the tail fat of 6-, 18-, and 30-month-old sheep (false discovery rate < 0.05, |Fold Change| ≥ 2). Based on the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, we found that fat-related DEGs were mainly expressed at 6 months of age, and gradually decreased at 18 and 30 months of age. The target gene prediction analysis shows that most of the lncRNAs target more than 20 mRNAs as their trans-regulators (53 mRNAs at most). Further, we obtained several fat-related differentially-expressed target genes; these target genes interact with different differentially expressed lncRNAs at various ages and play an important role in the development of tail fat. Based on the DEGs and differentially expressed lncRNAs, we established three co-expression networks for each comparison group. Conclusions: Finally, we conclude that the development of the sheep tail fat is more active during the early stage of growth and gradually decreases with the increase in age. The mutual regulation of lncRNAs and mRNAs may play a key role in this complex biological process, and our findings will provide some basic theoretical data for future studies on tail fat development of fat-tailed sheep.

KEGG

RNA-Seq

10.21203/rs.3.rs-131480/v1

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Citations (5)