RS

Rasoul Salehi

General Motors (United States)

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Mansour Salehi

Mashhad University of Medical Sciences

Gilda Eslami

Shahid Sadoughi University of Medical Sciences and Health Services

Bahram Pakzad

Isfahan University of Medical Sciences

Mohammad Kazemi

Isfahan University of Medical Sciences

Sharifeh Khosravi

Isfahan University of Medical Sciences

Masoud Etemadifar

Isfahan University of Medical Sciences

Fereshteh Alsahebfosoul

Isfahan University of Medical Sciences

Mansour Salesi

Isfahan University of Medical Sciences

Amirhossein Salehi

Shahid Beheshti University of Medical Sciences

Ali Doostmohammadi

Semnan University of Medical Sciences

Cooperative Institutions

Isfahan University of Medical Sciences

University of Isfahan

Tehran University of Medical Sciences

Shahid Beheshti University of Medical Sciences

University of Tehran

Shiraz University of Medical Sciences

Iran University of Medical Sciences

Shahrekord University of Medical Sciences

Alzahra Medical and Education Center

Tarbiat Modares University

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Construction and Characterization of Recombinant HEK Cell Over-Expressing TOSO/FAIM3 and Evaluation of its Expression

DOAJ (DOAJ: Directory of Open Access Journals) (2015)

Nahid Heidari-Hafshejani Shamsi Naderi Rasoul Salehi Parvaneh Nikpour Mehran Modares-Sadeghi

HEK 293 cells

Characterization

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Citations (0)

Detection of Mutation in Exons 3 and 8 of SLC3A1 and Exons 4 and 10 of SLC7A9 Genes in Patients with Cystinuria in Iran

DOAJ (DOAJ: Directory of Open Access Journals) (2014)

Leila Koulivand Mehrdad Mohammadi Sichani Rasoul Salehi Behrouz Ezatpour Majid Kheirollahi

Cystinuria

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Determining Educational Objectives and Evaluating Them in Department of Anatomy in Isfahan School of Medicine

Iranian Journal of Medical Education (2011)

Rasoul Salehi Najmeh Jafari Golshani Marjan Haghani Fariba

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Effects of Lactobacillus reuteri-derived biosurfactant on the gene expression profile of essential adhesion genes (gtfB, gtfC and ftf) of Streptococcus mutans

Advanced Biomedical Research (2014)

Rasoul Salehi Omid Savabi Mohammad Kazemi Sara Kamali AhmadReza Salehi

Streptococci are the main causative agents in plaque formation and mutans streptococci are the principle etiological agent of dental plaque and caries. The process of biofilm formation is a step-wise process, starting with adhesion of planktonic cells to the surfaces. It is now a well known fact that expression of glucosyltransferases (gtfs) and fructosyltransferase (ftf) genes play a critical role in the initial adhesion of Streptococcus mutans to the tooth surface, which results in the formation of dental plaques and consequently caries and other periodontal diseases. In the present study, we have determined the effect of biosurfactants purified from Lactobacillus reuteri (DSM20016) culture on gene expression profile of gftB/C and fft of S. mutans (ATCC35668) using quantitative real-time polymerase chain reaction. The application of biosurfactant caused considerable down-regulation of the expression of all three genes under study. The reduction in gene expression was statistically very significant (P > 0.0001 for all three genes). Inhibition of these genes by the extracted L. reuteri biosurfactant shows the emergence of a powerful alternative to the presently practicing alternatives. In view of the importance of these gene products for S. mutans attachment to the tooth surface, which is the initial important step in biofilm production and dental caries, we believe that the biosurfactant prepared in this study could be considered as a step ahead in dental caries prevention.

Lactobacillus reuteri

10.4103/2277-9175.139134

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Citations (43)

Single Nucleotide Polymorphism rs2075876 in AIRE Gene is a Strong Rheumatoid Arthritis Determinant

ACTA MEDICA IRANICA (2021)

Rasoul Salehi Mansour Salesi Elham Noormohamadi Bahram Pakzad Farzaneh Yousefisadr

Rheumatoid arthritis (RA) is a progressive and common autoimmune disease with multifactorial etiology. Several pieces of research show that genetic factors play a major role in the incidence of RA. Several genome-wide association studies (GWAS) have identified the autoimmune regulator (AIRE) gene as one of the candidate loci. This gene encodes a transcription factor, which is involved in the presentation of self-antigens and the negative selection of self-reactive T-cells in the thymus. Studies have indicated that single nucleotide polymorphisms (SNPs) in the AIRE gene can change the gene expression and/or function. In the present study, we assessed the possible association between SNP rs2075876 (intronic variant) in the AIRE gene with RA risk in the Iranian population. A case-control study using 56 RA patients and 58 control subjects was undertaken to evaluate rs2075876 genotypes using the real-time PCR high resolution melting method (HRM). Logistic regression analysis demonstrates that homozygous AA and heterozygous AG genotypes compared with GG genotype increase the risk of RA (AA vs. GG; OR=16.43; 95% CI [5.33-50.71] and AG vs. GG; OR=3.21; 95% CI [1.22-8.45]). Also, individuals with allele A were more frequently affected with RA than subjects with G allele (OR=5.81; 95% CI [3.28-10.30]). Furthermore, in the patient group, we found a significant correlation between erythrocyte sedimentation rate and C-reactive protein concentration with rs2075876 polymorphism (P<0.05). Our findings propose a substantial correlation between rs2075876 polymorphism and RA risk.

SNP

High Resolution Melt

Genome-wide Association Study

Erythrocyte sedimentation rate

10.18502/acta.v59i5.6660

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ISOLATION OF BRUCELLA ABORTUS USING PCR-RFLP ANALYSIS

Majallah-i bihdāsht-i Īrān (2006)

Mansour Salehi Rasoul Salehi E Pishva Mohammad Reza Rahmani

Brucella transmission and epidemiology depend on infecting species and biovar. Therefore, exact identification of the Brucella is important to design correct control and treatment strategies. In this study, we examined presence of other Brucellae in Isfahan. One hundred twenty Brucella isolates were collected and genomic DNA was extracted from them. omp2a fragment of all isolates were amplified using a pair of specific primers and the PCR products were electrophoresed and stained with EtBr. These PCR products were then restricted using PstI restriction endonuclease. The PCR products of all isolates had the same size of 1100bp. The banding pattern of PCR-RFLP for all of the isolates were similar to banding pattern of the Brucella melitensis biotype 1 except for 5 samples that demonstrated banding pattern similar to B. abortus. Based on our results, it is clear that biotype 1 of the B. melitensis is not the only Brucella present in Isfahan and now B. abortus is also present in our area. These results are very important in planning for the control of the disease as well epidemiology and even treatment of the patients.

Biovar

PstI

genomic DNA

Brucellaceae

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CORRELATION OF CAGA POSITIVE HELICOBACTER PYLORI INFECTION WITH CLINICAL OUTCOMES IN ALZAHRA HOSPITAL, ISFAHAN, IRAN

H Ghasemian Safaei Tavakoli Hamid Fereidoon Mojtahed Jaberi Rasoul Salehi Soleymani Bahram

BACKGROUND: Helicobacter pylori causes chronic active gastritis, peptic ulcer, non-cardia gastric cancer and mucosal- associated lymphoid tissue (MALT) lymphoma. Different genotypes of Helicobacter pylori are confirmed from disease geographical areas. Its association with clinical disease remained controversial. The aim of the present study was to investigate the relationship of the cagA genotype of Helicobacter pylori isolates with clinical manifestations and its re- lation to age and sex of patients. METHODS: A total of 100 patients (60 male and 40 female) biopsy specimens were obtained from 3 groups of patients (40 chronic active gastritis, 40 duodenal ulcers and 20 non-gastric gastric cancers). Biopsies were cultured on specific medium and after growth colonies were confirmed as Helicobacter pylori. DNA extraction and polymerase chain reac- tion (PCR) were used to detect the presence or absence of cagA gene. RESULTS: From a total of 100 positive samples of H. pylori, cagA genes were detected in 68% of patients and 32% of samples were negative. Mean age of normal gastritis, duodenal ulcer and gastric adenocarcinoma was 44.94, 44.97 and 67.5 years, respectively. CONCLUSIONS: The present study showed no significant relationship between cagA genotype of H. pylori and chronic active gastritis, duodenal ulcer and non-cardia gastric cancer as well as sex of patients. But, in gastric adenocarcinoma, there was significant discrepancy between ages of patients in comparison with the other two groups.

CagA

Chronic gastritis

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Citations (21)

Association analysis of −416 G>C polymorphism of T‐cell immunoglobulin and mucin domain‐1 gene with asthma in Iran

International Journal of Immunogenetics (2015)

Hedayatollah Shirzad Rezvan Meshkat Mazdak Ganjalikhani‐Hakemi Ali Mosayebian Ramin Ghasemi

Summary TIM (T‐cell immunoglobulin (Ig) and mucin domain)‐1, one of the members of TIM family, expresses on Th2 cells and promotes the production of Th2 signature cytokines. This can increase a series of responses in these cells which could be one of the causes of asthma or asthma‐related phenotypes. The aim of this study was to investigate whether a TIM ‐1 promoter single nucleotide polymorphism ( SNP ), −416 G>C, is associated with asthma in Iranian population. In this case–control study, existence of the −416 G>C polymorphism was assessed using polymerase chain reaction ( PCR ) and restriction fragment length polymorphism ( RFLP ) in 300 patients with asthma (97 atopic, 203 nonatopic) and 309 healthy volunteers. Additionally, the relationship between these polymorphism genotypes and total serum IgE levels in this Iranian population was evaluated. We discovered a significant association between the −416 G>C polymorphism and atopic asthma susceptibility in the population, but this SNP showed no connection with nonatopic asthma ( P < 0.05). However, our results showed significant relation between this polymorphism and serum IgE level ( P < 0.05). Our results suggest that −416 G>C polymorphism in TIM ‐1 gene could be a predisposing factor for atopic asthma in Iranian population, and CC genotype of this SNP can be associated with increased level of IgE in patients with asthma in the same population.

10.1111/iji.12209

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Genetic analysis of cardiac SCN5A Gene in Iranian patients with hereditary cardiac arrhythmias

The Anatolian Journal of Cardiology (2015)

Marzi Asadi Roger Foo Zahurul A. Bhuiyan Mohammad Reza Samienasab Ahmad Salehi

SCN5A encodes alpha subunit of the major sodium channel (Nav1.5) in human cardiac tissue. Malfunction of this cardiac sodium channel is associated with a variety of cardiac arrhythmias and myocardial inherited diseases.Fifty-three members from three families each diagnosed with long-QT syndrome type 3 (LQTS3), Brugada syndrome (BrS), or sick sinus syndrome (SSS) were included in this observational, cross-sectional study. In this study, we analyzed the sequences of coding region of the SCN5A gene.Eleven members of the LQTS family (39%) showed p.Gln1507-Lys1508-Pro1509del mutation, 8 of BrS family (50%) showed p.Arg222Ter nonsense mutation, and 5 of 9 SSS family members (55%) showed a novel p.Met1498Arg mutation in the SCN5A gene.p.Gln1507-Lys1508-Pro1509del mutation, p.Arg222Ter nonsense mutation, and p.Met1498Arg in LQTS, BrS, and SSS, respectively, are reported for the first time in the Iranian population. Information regarding underlying genetic defects would be necessary for verifying certain clinically diagnosed arrhythmia types, carrier screening in affected families, and more precise therapy of the patients are required.

Nonsense mutation

SSS*

Sick sinus syndrome

Nonsense

10.5152/akd.2015.6060

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Citations (3)

The correlation between Pax5 deletion and patients survival in Iranian children with precursor B-cell acute lymphocytic leukemia

Cellular and Molecular Biology (2017)

Alireza Moafi A. Zojaji Rasoul Salehi Somayeh Najafi Dorcheh Soheila Rahgozar

Despite advances in treatment, children with acute lymphoblastic leukemia (ALL) still experience drug resistance and relapse. Several gene mutations are involved in the onset of this disease and resistance to therapy. The present study examines the incidence of IKZF1, CDKN2A/B, PAX5, EBF1, ETV6, BTG1, RB1, JAK2, and Xp22.33 gene deletions/duplications associated with pediatric ALL in Iran and investigates the possible effect of these mutations on drug resistance. Three-year disease-free survival (3DFS) was evaluated for children diagnosed with Philadelphia negative precursor-B-cell ALL hospitalized at Sayed-al-Shohada Hospital, Isfahan-Iran, from January 2009 until December 2012. DNA was extracted from bone marrow slides, and ALL correlated gene deletions and duplications were measured using Multiplex Ligation-dependent Probe Amplification (MLPA) method. The correlation between gene mutations and 3DFS was then assessed. Among the nine aforementioned investigated genes, 63% of samples showed at least one gene mutation. At least two concomitant genomic mutations were observed in 42% of samples. Pax5 deletion was the most prevalent gene mutation observed in 45% of cases, and showed significant negative impact on response to treatment. CDKN2A/B (9p21.3) gene deletion, and ETV6 (12p13.2) gene duplication also demonstrated negative effect on patient survival and contributed to a worse prognosis if concomitant with Pax5 gene deletion. ALL patients with one of the gene deletions including Pax5 and CDKN2A/B (9p21.3) or ETV6 (12p13.2) gene duplication are classified as high-risk patients and need more intensified protocols of treatment to improve their chance of survival.

CDKN2B

Amplicon

ETV6

PAX5

10.14715/cmb/2017.63.8.4

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Citations (7)