Hiroshi Hasegawa

Utsunomiya University

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Hiroyuki Takano

National Defense Academy of Japan

Issei Komuro

The University of Tokyo

Yasunori Kanaho

University of Tsukuba

Hiroyuki Tadokoro

Tokyo University of Pharmacy and Life Sciences

Jiuhou Lei

University of Science and Technology of China

M. J. Engebretson

Augsburg University

Xing Li

Jilin University

S Sridharan

GlobalFoundries (United States)

A. V. Artemyev

University of California, Los Angeles

M. M. Cowee

Los Alamos National Laboratory

Cooperative Institutions

The University of Tokyo

146

Osaka University

144

Charles River Laboratories (Netherlands)

Kyoto University

Chiba University

Nagoya University

Keio University

Kobe University

Kyushu University

Tohoku University

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High incidence of pre‐excitation syndrome in Japanese families with Leber's hereditary optic neuropathy

Clinical Genetics (1996)

Yukihiko Mashima K Kigasawa Hiroshi Hasegawa Masato Tani Yoshihisa Oguchi

Cardiac conduction abnormalities have been reported in families with Leber's hereditary optic neuropathy (LHON). The pre‐excitation syndrome, Wolff‐Parkinson‐White syndrome or Lown‐Ganong‐Levine syndrome, is reportedly common in Finns with LHON, being seen in 14 (9%) of the 163 individuals with mitochondrial DNA (mtDNA) mutations. While this syndrome is thought to be rare in other ethnic groups with LHON, the present study of 35 Japanese LHON families confirmed that it is also relatively common among Japanese families, being seen in 5 (8%) of the 63 individuals with mtDNA mutations. It remains to be determined whether the high incidence of the pre‐excitation syndrome in Finnish and Japanese LHON families is due to a particular genetic composition of ethnic groups such as in Finland and in Japan, or only to a reporting bias.

10.1111/j.1399-0004.1996.tb02732.x

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Citations (59)

A Granulocyte-colony Stimulating Factor Producing Undifferentiated Hepatocarcinoma

The Japanese Journal of Gastroenterological Surgery (2015)

Takashi Komatsubara Koji Fujimoto Kengo Asari Hiroshi Hasegawa Hideaki Nishigori

症例は76歳の男性で，倦怠感を主訴に受診した．血液検査にて白血球数が37,100/μlと上昇し，腹部造影CTにて肝右葉に辺縁に造影効果のある径8 cmの腫瘤を認めた．まず肝膿瘍を疑い，膿瘍ドレナージを含めた保存的加療を開始したが改善傾向を認めなかった．その後白血球数が71,000/μlと異常高値を示したためgranulocyte-colony stimulating factor（以下，G-CSFと略記）を測定したところ，800 pg/ml（基準値<39 pg/ml）と上昇を認め，G-CSF産生腫瘍を疑い手術を行った．病理組織学的検査は肝未分化癌で，G-CSF免疫染色陽性でありG-CSF産生肝未分化癌と診断した．手術後，白血球数は一旦正常化したが再び上昇し，次第に全身状態不良となり術後32日目に死亡した．非常にまれなG-CSF産生肝未分化癌を経験したので報告する．

10.5833/jjgs.2014.0167

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Citations (4)

Use of ELISA to Quantify Magnaporthe grisea in a Rice Leaf.

Japanese Journal of Phytopathology (1999)

Hiroshi Hasegawa J. Miyazawa Michiaki Iwata

We produced a polyclonal antibody which reacts with antigens of mycelia of the rice blast fungus. Using this antibody, we developed an ELISA method which can measure small amounts of mycelia of Magnaporthe grisea (Hebert) Barr in a rice leaf. More than 0.1μg mycelia of the rice blast fungus in 100mg of fresh leaf can be measured by this method. The antibody, acquired using lyophilized mycelia of the fungus, reacts as well with fresh mycelia, conidia, and even those of different races as it does with lyophilized ones. But it does not react with mycelia of several fungal pathogens of the rice plant. Thanatephorus cucumeris (Frank) Donk, Rhizoctonia sp., Cochliobolus miyabeanus (Ito et Kuribayashi) Drechsler ex Dastur, Sphaerulina oryzina Hara and Nigrospora oryzae (Berkeley et Broome) Petch do not react with the antibody at 20μg dry weight mycelia/ml of PBS (pH 7.5) containing 0.05% Tween 80. But at about 2mg/ml, the antibody slightly reacts with mycelia of Sphaerulina oryzina and Nigrospora oryzae. This method should be useful in understanding the interaction between blast fungus and rice plant.

Rhizoctonia

Pathogenic fungus

Appressorium

10.3186/jjphytopath.65.312

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Citations (2)

1D03-5 Functional role of hypothalamic dopamine on endurance exercise capacity in rats(The Proceedings of the 22nd Annual Meetings of Japan Society of Exercise and Sports Physiology July 19・20, (Okayama)) :

Advances in exercise and sports physiology (2014)

Hiroshi Hasegawa

Endurance Training

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B-PO03-141 A CONVERSION TO/FROM ATYPICAL ATRIOVENTRICULAR NODAL REENTRANT TACHYCARDIA USING A SUPERIOR SLOW PATHWAY OWING TO A SHIFT OF CIRCUIT AFTER ABLATION

Heart Rhythm (2021)

Shuntaro Tamura Tadashi Nakajima Hiroshi Hasegawa Takashi Kobari Kazuya Nakagawa

Reentrancy

Reentry

Atrioventricular node

10.1016/j.hrthm.2021.06.615

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Evaluation of screening tests for autoimmune gastritis in histopathologically confirmed Japanese patients, and re-evaluation of histopathological classification

Research Square (Research Square) (2022)

Yasuhiro Wada Shigemi Nakajima Naoko Mori Shizuki Takemura Rena Chatani

Abstract Background : The aims of the present study are to evaluate non-invasive screening tests for autoimmune gastritis (AIG) and re-evaluate histopathological classification. Methods : We screened candidates of AIG in JCHO Shiga Hospital between May 2012 and January 2020. The screening criteria were as follows: endoscopic O-p atrophy with Updated Kimura-Takemoto classification, 3+ pepsinogen (PG) test, low serum vitamin B 12 or elevated serum gastrin with positive anti-parietal cell (PC) or intrinsic factor antibodies. We evaluated the screening criteria in the patients who were histopathologically confirmed as AIG, and re-evaluated histopathological staging in clinical aspects. Results : Twenty-two of 28 (78.6%) patients who met the screening criteria were histopathologically confirmed as AIG. Common clinical findings in the AIG patients were 10x or greater anti-PC antibody, elevated serum gastrin greater than 172 pg/mL and endoscopic atrophy O-1 or greater. The areas under the curve of PG I, PG II and PG I/II ratio were 0.81, 0.29 and 0.98, respectively. Among histopathologically confirmed AIG patients, 4 and 18 patients were histopathologically classified into florid and end stages, respectively, while no patients into early stage. We could not find a significant difference between florid and end stages in the screening items studied. Conclusions : Florid and end stages in histopathological classification are both advanced-stage AIG in clinical aspects. Our screening criteria without biopsy are applicable to screen clinically-advanced AIG with 78.6% positive predictive value. PG I and PG I/II ratio may be useful to screen AIG. However, we may need other criteria to screen early stage of AIG.

Atrophic gastritis

Autoimmune Gastritis

10.21203/rs.3.rs-1210431/v1

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Citations (0)

Tokyo Centenarian Study. 4. Apolipoprotein E Phenotype in Japanese Centenarians Living in the Tokyo Metropolitan Area.

Nippon Ronen Igakkai Zasshi Japanese Journal of Geriatrics (1997)

Nobuyoshi Hirose Satoki Homma Yasumichi Arai Masahide Kawamura Hiroshi Hasegawa

アポリポ蛋白E (以下アポEと略す) の phenotype が寿命にどの様な影響を及ぼすかを検討する目的で百寿者のアポE phenotype を検討した. 対象は東京在住の百寿者54名でアポE phenotype および allele の頻度分布を調べた. 対照群は文献的に収集した883名に慶応健康相談センター受診者90名を加えた973名とした. 百寿者のアポE phenotype はE2/E2 2名 (3.7%), E2/E3 5名 (9.3%), E3/E3 38名 (70.4%), E3/E4 9名 (16.6%) であり他の phenotype は観察されなかった. 一方対照群ではE2/E2 0.2%, E2/E3 5.8%, E3/E3 73.2%, E3/E4 18.4%であり, 百寿者群ではE2/E2およびE2/E3が多かった. アポEの allele について百寿者と対照群で比較するとε2 8.3% vs 3.6%, ε3 83.4% vs 85.4%, ε4 8.3% vs 10.9%でありχ2検定を行うとχ2値6.841, p値0.0327と有意に発現頻度が異なった. これは百寿者群ではε2の頻度が高いためと考えられる. アポEの phenotype による血中脂質の違いを検討したところE2/E2+E2/E3群ではE3/E3群に比較し有意にコレステロール値, アポ蛋白B値, 低比重リポ蛋白コレステロール値が低下していた. またアポE濃度は有意に高値を示した. 日本の百寿者87名, フランス人百寿者325名およびフィンランド人百寿者179名においてアポE allele の発現頻度を検討した. どの人種でもε2の頻度は対照群の約2倍でありε4は約1/2となっていた. 各群での allele の違いを検討するとフランス百寿者でε2の頻度が高かったがそれ以外はほぼ同じ頻度を示した.上記よりアポE phenotype は寿命に関与する可能性が示唆された.

Centenarian

Apolipoprotein E

10.3143/geriatrics.34.267

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Citations (20)

Effects of Choline Administration on In Vivo Release and Biosynthesis of Acetylcholine in the Rat Striatum as Studied by In Vivo Brain Microdialysis

Journal of Neurochemistry (1990)

Kunio Koshimura Soichi Miwa Ken Lee Yasunori Hayashi Hiroshi Hasegawa

Abstract: The purpose of the present study is to clarify the effects of the administration of choline on the in vivo release and biosynthesis of acetylcholine (ACh) in the brain. For this purpose, the changes in the extracellular concentration of choline and ACh in the rat striatum following intracerebroventricular administration of choline were determined using brain microdialysis. We also determined changes in the tissue content of choline and ACh. When the striatum was dialyzed with Ringer solution containing 10 μM physostigmine, ACh levels in dialysates rapidly and dose dependently increased following administration of various doses of choline and reached a maximum within 20 min. In contrast, choline levels in dialysates increased after a lag period of 20 min following the administration. When the striatum was dialyzed with physostigmine‐free Ringer solution, ACh could not be detected in dialysates both before and even after choline administration. After addition of hemicholinium‐3 to the perfusion fluid, the choline‐induced increase in ACh levels in dialysates was abolished. Following administration of choline, the tissue content of choline and ACh increased within 20 min. These results suggest that administered choline is rapidly taken up into the intracellular compartment of the cholinergic neurons, where it enhances both the release and the biosynthesis of ACh.

Microdialysis

Choline

10.1111/j.1471-4159.1990.tb01904.x

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Citations (62)

Arf6 regulates tumour angiogenesis and growth through HGF-induced endothelial β1 integrin recycling

Nature Communications (2015)

Tsunaki Hongu Yuji Funakoshi Shigetomo Fukuhara Teruhiko Suzuki Susumu Sakimoto

Anti-angiogenic drugs targeting vascular endothelial cell growth factor receptor have provided modest clinical benefit, in part, owing to the actions of additional angiogenic factors that stimulate tumour neoangiogenesis in parallel. To overcome this redundancy, approaches targeting these other signalling pathways are required. Here we show, using endothelial cell-targeted mice, that the small GTPase Arf6 is required for hepatocyte growth factor (HGF)-induced tumour neoangiogenesis and growth. Arf6 deletion from endothelial cells abolishes HGF-stimulated β1 integrin recycling. Pharmacological inhibition of the Arf6 guanine nucleotide exchange factor (GEF) Grp1 efficiently suppresses tumour vascularization and growth. Grp1 as well as other Arf6 GEFs, such as GEP100, EFA6B and EFA6D, regulates HGF-stimulated β1 integrin recycling. These findings provide insight into the mechanism of HGF-induced tumour angiogenesis and offer the possibility that targeting the HGF-activated Arf6 signalling pathway may synergize with existing anti-angiogenic drugs to improve clinical outcomes. Targetting tumour angiogenesis is a useful strategy to reduce tumour burden; however, the clinical benefits of anti-angiogenetic drugs are modest. Here, the authors show that HGFR signalling, which contributes to tumour angiogenesis, requires Arf6 and that blocking Arf6 can lead to reduced tumour growth in mice.

10.1038/ncomms8925

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Citations (63)

Effects of amino acids and chirality for molecular folding of desoxazoline-ascidiacyclamide derivatives: X-ray crystal structures of four cyclic octapeptides including unusual amino acids,cyclo(-Ile-aThr-D-Val-Thz-)2,cyclo(-Ala-aThr-D-Val-Thz-Ile-aThr-D-Val-Thz-),cyclo(-Val-aThr-D-Val-Thz-Ile-aThr-D-Val-Thz-), andcyclo(-Ile-aThr-Val-Thz-Ile-aThr-D-Val-Thz-)

Biopolymers (2001)

Akiko Asano Mitsunobu Doi Kiyomi Kobayashi Masao Arimoto Toshimasa Ishida

Desoxazoline derivative of ascidiacyclamide (1), cyclo(-L-Ile-L-allo-threonine-D-Val-thiazole-)(2), was modified to disturb the C(2)-symmetry. An Ile(1) residue of 1 was replaced for Ala (2) or Val (3), and the D-Val(3) residue was replaced for Val (4). The crystal structures of 1-4 were analyzed by x-ray diffraction methods. The molecules of all compounds were folded and this type of structure was not observed in x-ray structures of ascidiacyclamide derivatives so far except for patellamide D. The folding patterns of 1-4 were similar to each other and resembled that of patellamide. The asymmetric modifications at position 1 caused the conformational changes at local area, and these were related with the peptide-peptide and peptide-solvent interactions. Despite the diverse backbone conformation by the epimeric modification at position 3, the entire molecule of 4 was folded. These results mean that (1) the desoxazoline-ascidiacyclamides favored the folded structures and (2) the modifications of the side chain size at position 1 and the chirality at position 3 brought the local conformational changes to derivatives, suggesting that (3) the lack of the oxazoline block leads to conformational flexibility of 1-4, which accepts the conformational change with no drastic change on the entire structure.

Chirality

Residue (chemistry)

Folding (DSP implementation)

Cyclic peptide

Peptide Conformation

10.1002/1097-0282(200103)58:3<295::aid-bip1006>3.0.co;2-x

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Citations (24)