Mohamed Ehab Ali

University of Bonn

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Alf Lamprecht

University of Bonn

Salma N. Tammam

German University in Cairo

M. Bharathidasan

Tamil Nadu Veterinary and Animal Sciences University

Maryam A. Shetab Boushehri

Ain Shams University

Mohamed Shafiuzama

Tamil Nadu Veterinary and Animal Sciences University

John Youshia

Ain Shams University

Ravi Sundar George

Tamil Nadu Veterinary and Animal Sciences University

Silvio Sollazzo

University of Cologne

G Shriram

C. V. Ramani

Tata Memorial Hospital

Cooperative Institutions

University of Bonn

Tamil Nadu Veterinary and Animal Sciences University

Inserm

Jamia Hamdard

German University in Cairo

Université de franche-comté

Université de Bourgogne

Université Bourgogne Franche-Comté

Observatoire des Sciences de l’Univers Terre Homme Environnement Temps Astronomie

Innovation Plasturgie Composites

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Formulation of PEG-ylated L-asparaginase loaded poly (lactide-co-glycolide) nanoparticles: influence of Pegylation on enzyme loading, activity and in vitro release.

PubMed (2011)

Suruchi S. Vasudev Sayeed Ahmad Rabea Parveen Farhan Jalees Ahmad C K Anish

The present paper describes the advantage of PEG-ylation of L-asparaginase before encapsulation over its incorporation in the native form. During encapsulation a considerable amount of native protein undergoes denaturation and forms insoluble aggregates. In an effort to overcome this problem, L-asparaginase was PEG-ylated before subjecting it to the harsh conditions as encountered during double emulsion solvent evaporation technique. L-asparaginase was conjugated with succinimidyl succinate derivative of polyethylene glycol (SS-PEG, MW 5000) followed by characterization of the formed conjugate using size exclusion-HPLC and SDS PAGE. The PEG-ylated L-asparaginase consisted of different isomers from mono to multi PEG-ylated depending upon the number of Lysine residues (14 in case of L-asparaginase) with about 5% as native protein. The specific activity as retained after PEG-ylation was 62.84 +/- 8.2% and further about 82.7% of activity was recovered from the particles. Imitated studies with the native protein confirmed the enhanced stability of the conjugated protein when exposed to the organic solvent and sonication and showed comparatively less encapsulation efficiency due to increased hydrophilicity. Release profiles for native as well as conjugated proteins consisted of sustained release of about 66.66% and 44.45% in 28 days, respectively. The decrease in the release can be attributed to the increase in the molecular weight of the conjugated protein. The study finally proved that PEG-ylation protected the enzyme and prevented it from denaturation during encapsulation.

PEGylation

Asparaginase

Sonication

Conjugate

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Citations (21)

Surgical management of massive uterine leiomyosarcoma with open cervix pyometra in a spitz bitch

Journal of Entomology and Zoology Studies (2020)

M. Gokulakrishnan Mala Shammi Lakshmi Nagarajan C. V. Ramani Mohamed Ehab Ali

A seven-year-old entire female, mixed-breed dog, weighing 19 kg, was presented with a 2-day history of abdominal distension, reduced appetite and general dullness. Abdominal palpation revealed a large mass. Radiography showed a large mass occupying the left mid-abdominal area and convoluted loops of tubular fluid opacity occupying the right mid-abdominal area. Ultrasonography revealed a large heterogeneous mass with an anechoic area and some hyperechoic foci, indicative of calcification, in the mid-abdominal area. Furthermore, hypoechoic areas were found in the middle and caudal abdominal area and were presumed to be the fluid-filled uterine horns. At laparotomy, a 10·5×14·5-cm firm mass was found in the uterine body, while the uterine horns were filled with a thick red-brownish exudate; ovariohysterectomy was subsequently performed. A diagnosis of uterine leiomyosarcoma associated with pyometra was established by histopathology.

Pyometra

Uterine horns

Abdominal mass

Abdominal distension

Palpation

Abdominal ultrasonography

Histopathology

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Citations (0)

Pulmonary delivery of anti-inflammatory agents

Expert Opinion on Drug Delivery (2014)

Mohamed Ehab Ali Jason T. McConville Alf Lamprecht

Introduction: Respiratory infections and diseases are accompanied by or exhibit inflammation. Recent advances in nanoparticle engineering technology, together with the increased knowledge of inflammatory pathophysiology, have ignited interest in the pulmonary delivery of anti-inflammatory agents (AIAs) to achieve local treatment of pulmonary inflammatory disorders.Areas covered: This review summarizes and discusses the investigated formulation approaches for the pulmonary delivery of AIAs, including: inhalation of actives as suspensions or dry powder formulations, with polymeric micro- and nano-delivery carriers, or within liposomes and lipid nanoparticles. Some recent approaches for targeting AIAs to the pulmonary endothelium have also been reviewed. The discussion focuses on finding out whether the investigated approaches were really able to achieve lung targeting and reduce the side effects associated with the systemic administration of AIAs.Expert opinion: The use of the inhalation route for the pulmonary delivery of AIAs is facing several challenges. Some of the investigated formulation approaches appear to be promising in overcoming these challenges. However, in order to create products that reach patients, more therapeutically oriented studies are still needed to ensure formulation stability, in-vivo sustained release behavior, pulmonary retention, and bypassing lung clearance mechanisms.

10.1517/17425247.2015.993968

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Citations (34)

Collagenase loaded chitosan nanoparticles for digestion of the collagenous scar in liver fibrosis: The effect of chitosan intrinsic collagen binding on the success of targeting

European Journal of Pharmaceutics and Biopharmaceutics (2020)

Sara El-Safy Salma N. Tammam Mohammad Abdel‐Halim Mohamed Ehab Ali John Youshia

Digestion

10.1016/j.ejpb.2020.01.003

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Citations (25)

Artificial neural network based particle size prediction of polymeric nanoparticles

European Journal of Pharmaceutics and Biopharmaceutics (2017)

John Youshia Mohamed Ehab Ali Alf Lamprecht

Dispersity

Particle (ecology)

10.1016/j.ejpb.2017.06.030

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Citations (89)

Spray freeze drying for dry powder inhalation of nanoparticles

European Journal of Pharmaceutics and Biopharmaceutics (2014)

Mohamed Ehab Ali Alf Lamprecht

Nanocarriers

Dry-powder inhaler

Aerosolization

Dispersity

Microparticle

Aerodynamic diameter

10.1016/j.ejpb.2014.03.009

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Citations (125)

Immune Modifying Effect of Drug Free Biodegradable Nanoparticles on Disease Course of Experimental Autoimmune Neuritis

Pharmaceutics (2022)

Ehsan Elahi Mohamed Ehab Ali Julian Zimmermann Daniel R. Getts Marcus Müller

Guillain-Barré Syndrome (GBS) is an autoimmune disease of demyelination and inflammation of peripheral nerves. Current treatments are limited to plasma exchange and intravenous immunoglobulins. Cargo-free nanoparticles (NPs) have been evaluated here for their therapeutic benefit on the disease course of experimental autoimmune neuritis (EAN), mimicking the human GBS. NPs prepared from poly-lactic co-glycolic acid (PLGA) with variable size and surface charge (i.e., 500 nm vs. 130 nm, polyvinyl alcohol (PVA) vs. sodium cholate), were intravenously administered in before- or early-onset treatment schedules in a rat EAN model. NP treatment mitigated distinctly the clinical severity of EAN as compared to the P2-peptide control group (P2) in all treatments and reduced the trafficking of inflammatory monocytes at inflammatory loci and diverted them towards the spleen. Therapeutic treatment with NPs reduced the expression of proinflammatory markers (CD68 (P2: 34.8 ± 6.6 vs. NP: 11.9 ± 2.3), IL-1β (P2: 18.3 ± 0.8 vs. NP: 5.8 ± 2.2), TNF-α (P2: 23.5 ± 3.7 vs. NP: 8.3 ± 1.7) and elevated the expression levels of anti-inflammatory markers CD163 (P2: 19.7 ± 3.0 vs. NP: 41.1 ± 6.5; all for NP-PVA of 130 nm; relative to healthy control). These results highlight the therapeutic potential of such cargo-free NPs in treating EAN, which would be easily translatable into clinical use due to their well-known low-toxicity profile.

Proinflammatory cytokine

Therapeutic effect

10.3390/pharmaceutics14112410

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Citations (5)

Adalimumab Decorated Nanoparticles Enhance Antibody Stability and Therapeutic Outcome in Epithelial Colitis Targeting

Pharmaceutics (2022)

Markus Ries Brice Moulari Maryam A. Shetab Boushehri Mohamed Ehab Ali Daniel Molnar

Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract with increasing incidence worldwide. Although a deeper understanding of the underlying mechanisms of IBD has led to new therapeutic approaches, treatment options are still limited. Severe adverse events in conventional drug therapy and poor drug targeting are the main cause of early therapy failure. Nanoparticle-based targeting approaches can selectively deliver drugs to the site of inflammation and reduce the risk of side effects by decreasing systemic availability. Here, we developed a nanoparticulate platform for the delivery of the anti-TNF-α antibody adalimumab (ADA) by covalent crosslinking to the particle surface. ADA binding to nanoparticles improved the stability of ADA against proteolytic degradation in vitro and led to a significantly better therapeutic outcome in a murine colitis model. Moreover, immobilization of ADA reduced systemic exposure, which can lead to enhanced therapeutic safety. Thus, nanoparticle protein decoration constitutes a platform through which epithelial delivery of any biological of interest to the inflamed gut and hence a local treatment can be achieved.

Targeted drug delivery

10.3390/pharmaceutics14020352

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Citations (10)

Nanoparticle formulations as recrystallization inhibitors in transdermal patches

International Journal of Pharmaceutics (2019)

Muhammad Azam Tahir Mohamed Ehab Ali Alf Lamprecht

Recrystallization (geology)

Ibuprofen

Nanocarriers

PLGA

Solid lipid nanoparticle